Rh4AG329000

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005622
Physical Location & Seq
Reverse (-)
63958515 .. 63966356
7842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG329000.1

Sequence Viewer

Length: 3636 bp
ATGCCCTCTTCACCATCTCCAATTCTTCTTCTTCTCCATCCCTCACCGCCTTCTCCACAATCACAATTCCCTTCGCGTCCCAAAGCCTCTGCCCTCAGACCCTCACCACTACTCGTCGCCATGAAGGACCGGCCTCCTTCTTCGTACGGCGCCGTTTACGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCACCAGCCCTAATTACACCTCCGCCGCCTCAATTGCCTCCAAGAAGACTACTGCTGCTGCTTCTCTCAGCAAAGCTCGCACCAATGGTGCTCGGACCTACTACCAGACTCAGCAGCACGAAGTTCAGGAACAGCTCCACAAGCCGAAGTTGCAGCACGATTCTGATTACGACGACGGCGGCGTCTCCGATGATGAAGGCTCCGATCGCGATTATAACATGTCGTCGAATCCGGGTGCTTCAATCTCTGATAACCTTGATGAGTGGAAACGGAAGTTAACCATGCTGTTACGTGACAACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGAGGACATAGCCGCTCTGGCAAGCAGAATGGGACTTTACAGCCATCTGTATGCAAAAGTTGCTGTATTCAGTAAGGTCCCACTGCCAAACTACAGATTTGATCTGGATGACCGGCGACCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCGTACCTCGGAGACTTCCTTTCTCAGAAGTCTAGGACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCTGAACCAGTGGCACCGAATAATGCTGCGATGGAAAATTTTTTTTGGAGGAGAAGCTTGCAGCTGCGGGATAAGCAACACGCTTGGCAGGAATCTCGTGAAGGCAGAAAAGTTATGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAAGGATGCTTTGTTGACAGCAATTTCACGAAATCAGGTTGTTATCATATCAGGGCAAACAGGCTGTGGAAAGACCACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGCCCGTGGAGCTGTATGTAGTATTATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCAGAAAGAGTTGCTTCAGAGAGAGGGGAGAAGTTGGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGCATGAAAGGGAGAGACACACGCCTTCTCTTCTGCACCACTGGCATTTTGTTGAGAAGATTGCTACTTGATGGAAATTTGAAAGGTGTAACTCATGTCATTGTTGATGAGATTCACGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTCCTGGAGGATGTTCTGGAAAGTACTGGTTGCAGGTTAACTCCATACAATCAGATTGACGATTATGGACAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAAGCAGCTGATTTTAAGGGTTACAGTCCCCAGACTCGGGAATCTCTAGCCTGTTGGAATCCTGATTGTATCGGTTTTAATCTCATAGAGTATCTGTTATGCAATATCTGTGAGAATGAAAGGCCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTAAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCAAGTCTTGTTGTTAGCATGCCATGGTTCCATGGCCAGTTCCGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGACGTTGTTTTTGTTATTGATTGTGGAAAGGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCTGGGCGTGTTCAACCTGGGGAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCATTTGCAGAATATCAATTGCCGGAAATCTTGAGGACACCTTTGCAGTCTCTTTGTCTGCAAATCAAAAGTTTAAAACTCGGAAGTATCTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTAGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAACGAGAATCTGACTATTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTCGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGAAAGATCTTGCGGAGGCTGCTAAATCTCAGTTTTCCCGAGACCACAGCGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCACCACAATCGATGAAAGCTATTGATTCCCTCAGGAAGGAGTTCTACTCTTTGCTAAGGGATACTGATCTTATCGATGCCAACACAGCTACCTATAATGTATGGAGCTATGATGAGCATCTCGTCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATTTGCTCTGTTATGCACAATGAGAAGTCATATTCACTGAAAACTATGGAGGATGGTCAGGTGCTGCTATACTCGAACTCTGTCAATGCTCGGGAATCTAAAATTCCATATCCATGGCTAGTTTTTAATGAGAAGATAAAGGTGAACTCTGTTTTTCTACGGGATTCAACAGCTGTATCTGATTCAGTGCTTCTCCTATTTGGTGGAAGTTTTTCAAAAGGGGATATTGATGGACACTTGAAAATGTTGGGTGGATACTTGGAATTCTTCATGAAACCTGCTGTAGCAGAAATGTATCAATGTTTAAGGGCAGAACTTGATGAGTTAATTCAAACCAAACTACGGAACCCAAGGATGGCCATCCACAAATATCATGAACTCCTTTCTGCAGTGCGGCTGCTGCTCTCGGAGGATCTATGTGAGGGTAGATTTGTATTTGGCCGCCAGGTTCGTACAACCTCAAAGCCATCTGTGGGGGCAGCTCAGCCAGGCTTGGTTTCAAGAACTGAAAGTGGACCTGGGGGTGATAATTCTAAGAGTCAGCTCCAGACATTGCTCACAAGGGCAGGATATGCTGCGCCCACCTACAAAACAATGCAACTAAAGAACCTCAAGTTCCAAGCTAGTGTGGAGTTCAATGGAATGCAAATTATGGGGCAGCCTTGTAATAACAAGAAAAGTGCAGAAAAAGATGCAGCAGCTGAGGCCATTCAGTGGCTTGTGAGTGGAACACAAATGGGCCATGACCACATCAATCACATGTCAATGATGTTGAAGAAAAGCAAAAAAGGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1211

Amino Acids

135.91

Weight (kDa)

8.66

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 329 - 481 1.2e-10 DEAD/DEAH box helicase
Helicase_C PF00271 588 - 716 9e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 780 - 808 6.1e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 809 - 868 5e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 935 - 1016 2.6e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1025 - 1083 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1125 - 1186 6.4e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 966
AasI GACNNNNNNGTC 1 cut(s) 371
Acc36I ACCTGC 2 cut(s) 1539, 3124
AccB1I GGYRCC 5 cut(s) 149, 835, 1615, 1793, 2456
AccB7I CCANNNNNTGG 2 cut(s) 2435, 3552
AccBSI CCGCTC 2 cut(s) 183, 551
AccII CGCG 3 cut(s) 76, 399, 1344
AclWI GGATC 5 cut(s) 1389, 1868, 2471, 2510, 3258
AcoI YGGCCR 4 cut(s) 1397, 1914, 3195, 3277
AcsI RAATTY 7 cut(s) 859, 1053, 1297, 2671, 2838, 2940, 3101
AcuI CTGAAG 1 cut(s) 1152
AcyI GRCGYC 2 cut(s) 150, 372
AfaI GTAC 6 cut(s) 146, 707, 799, 1120, 1540, 3292
AflIII ACRYGT 2 cut(s) 408, 3597
AjiI CACGTC 1 cut(s) 530
AjnI CCWGG 9 cut(s) 1486, 1518, 1789, 2096, 2152, 2833, 3282, 3325, 3355
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AloI GAACNNNNNNTCC 2 cut(s) 1194, 1226
Alw21I GWGCWC 1 cut(s) 283
AlwI GGATC 5 cut(s) 1389, 1868, 2471, 2510, 3258
AlwNI CAGNNNCTG 2 cut(s) 2902, 3539
Ama87I CYCGRG 3 cut(s) 1701, 2577, 2928
ApoI RAATTY 7 cut(s) 859, 1053, 1297, 2671, 2838, 2940, 3101
ArsI GACNNNNNNTTYG 2 cut(s) 2006, 2038
AseI ATTAAT 1 cut(s) 2010
Asp700I GAANNNNTTC 4 cut(s) 750, 1359, 2720, 3049
AspA2I CCTAGG 1 cut(s) 1127
AspLEI GCGC 2 cut(s) 152, 3418
AspS9I GGNCC 6 cut(s) 127, 285, 613, 2364, 3353, 3577
AsuC2I CCSGG 1 cut(s) 423
AsuII TTCGAA 1 cut(s) 1507
AvaI CYCGRG 3 cut(s) 1701, 2577, 2928
AvaII GGWCC 4 cut(s) 127, 285, 613, 3353
AvrII CCTAGG 1 cut(s) 1127
AxyI CCTNAGG 2 cut(s) 1950, 2711
BaeI ACNNNNGTAYC 2 cut(s) 2997, 3030
BalI TGGCCA 2 cut(s) 1916, 3197
BanI GGYRCC 5 cut(s) 149, 835, 1615, 1793, 2456
BanII GRGCYC 3 cut(s) 813, 1471, 2305
BarI GAAGNNNNNNTAC 2 cut(s) 2708, 2740
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 283
BbvCI CCTCAGC 2 cut(s) 689, 3540
BceAI ACGGC 3 cut(s) 137, 163, 382
BcgI CGANNNNNNTGC 4 cut(s) 899, 933, 2579, 2613
BciT130I CCWGG 9 cut(s) 1488, 1520, 1791, 2098, 2154, 2835, 3284, 3327, 3357
BciVI GTATCC 3 cut(s) 2734, 2841, 3086
BcnI CCSGG 1 cut(s) 423
BfmI CTRYAG 4 cut(s) 628, 2306, 3120, 3225
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 1539, 3124
BfuI GTATCC 3 cut(s) 2734, 2841, 3086
BglI GCCNNNNNGGC 1 cut(s) 554
BglII AGATCT 1 cut(s) 2545
BlnI CCTAGG 1 cut(s) 1127
BlpI GCTNAGC 1 cut(s) 3321
BmcAI AGTACT 1 cut(s) 1540
Bme18I GGWCC 4 cut(s) 127, 285, 613, 3353
BmeT110I CYCGRG 3 cut(s) 1701, 2577, 2928
BmgBI CACGTC 1 cut(s) 530
BmgT120I GGNCC 6 cut(s) 127, 285, 613, 2364, 3353, 3577
BmrI ACTGGG 1 cut(s) 1824
BmsI GCATC 8 cut(s) 967, 1648, 2053, 2182, 2433, 2746, 2806, 3520
BmuI ACTGGG 1 cut(s) 1824
BpiI GAAGAC 1 cut(s) 242
BplI GAGNNNNNCTC 4 cut(s) 1433, 1465, 2711, 2743
BpmI CTGGAG 2 cut(s) 1541, 3368
Bpu10I CCTNAGC 3 cut(s) 689, 2735, 3540
Bpu1102I GCTNAGC 1 cut(s) 3321
Bpu14I TTCGAA 1 cut(s) 1507
BpuEI CTTGAG 3 cut(s) 1361, 2245, 3434
BpuMI CCSGG 1 cut(s) 423
Bsa29I ATCGAT 2 cut(s) 2690, 2754
BsaAI YACGTR 1 cut(s) 482
BsaBI GATNNNNATC 3 cut(s) 2745, 2796, 3197
BsaHI GRCGYC 2 cut(s) 150, 372
BsaI GGTCTC 1 cut(s) 2574
Bse118I RCCGGY 3 cut(s) 129, 648, 1395
Bse1I ACTGG 5 cut(s) 830, 1267, 1546, 1819, 1917
Bse21I CCTNAGG 2 cut(s) 1950, 2711
Bse3DI GCAATG 1 cut(s) 3389
Bse8I GATNNNNATC 3 cut(s) 2745, 2796, 3197
BseBI CCWGG 9 cut(s) 1488, 1520, 1791, 2098, 2154, 2835, 3284, 3327, 3357
BseCI ATCGAT 2 cut(s) 2690, 2754
BseJI GATNNNNATC 3 cut(s) 2745, 2796, 3197
BseMI GCAATG 1 cut(s) 3389
BseNI ACTGG 5 cut(s) 830, 1267, 1546, 1819, 1917
BseRI GAGGAG 2 cut(s) 706, 886
BseYI CCCAGC 2 cut(s) 958, 2138
BsgI GTGCAG 3 cut(s) 1240, 2829, 3540
Bsh1236I CGCG 3 cut(s) 76, 399, 1344
Bsh1285I CGRYCG 1 cut(s) 397
BshNI GGYRCC 5 cut(s) 149, 835, 1615, 1793, 2456
BshVI ATCGAT 2 cut(s) 2690, 2754
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 3 cut(s) 1701, 2577, 2928
BsiSI CCGG 5 cut(s) 130, 422, 649, 1396, 2216
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 5 cut(s) 63, 146, 582, 599, 1677
BsmBI CGTCTC 3 cut(s) 379, 1988, 2174
BsmFI GGGAC 5 cut(s) 63, 146, 582, 599, 1677
BsmI GAATGC 1 cut(s) 3486
Bso31I GGTCTC 1 cut(s) 2574
BsoBI CYCGRG 3 cut(s) 1701, 2577, 2928
Bsp119I TTCGAA 1 cut(s) 1507
Bsp1286I GDGCHC 4 cut(s) 283, 813, 1471, 2305
Bsp1407I TGTACA 1 cut(s) 1118
Bsp1720I GCTNAGC 1 cut(s) 3321
Bsp19I CCATGG 3 cut(s) 1903, 1911, 2951
Bsp68I TCGCGA 1 cut(s) 399
BspDI ATCGAT 2 cut(s) 2690, 2754
BspFNI CGCG 3 cut(s) 76, 399, 1344
BspHI TCATGA 2 cut(s) 3108, 3211
BspMAI CTGCAG 1 cut(s) 3229
BspMI ACCTGC 2 cut(s) 1539, 3124
BspPI GGATC 5 cut(s) 1389, 1868, 2471, 2510, 3258
BspQI GCTCTTC 1 cut(s) 2128
BspT104I TTCGAA 1 cut(s) 1507
BspT107I GGYRCC 5 cut(s) 149, 835, 1615, 1793, 2456
BspTNI GGTCTC 1 cut(s) 2574
BsrBI CCGCTC 2 cut(s) 183, 551
BsrDI GCAATG 1 cut(s) 3389
BsrFI RCCGGY 3 cut(s) 129, 648, 1395
BsrGI TGTACA 1 cut(s) 1118
BsrI ACTGG 5 cut(s) 830, 1267, 1546, 1819, 1917
BssAI RCCGGY 3 cut(s) 129, 648, 1395
BssNI GRCGYC 2 cut(s) 150, 372
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 7 cut(s) 1127, 1903, 1911, 2106, 2367, 2951, 3188
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 9 cut(s) 1488, 1520, 1791, 2098, 2154, 2835, 3284, 3327, 3357
Bst4CI ACNGT 2 cut(s) 1691, 2310
Bst6I CTCTTC 4 cut(s) 13, 1256, 2122, 2128
BstACI GRCGYC 2 cut(s) 150, 372
BstAPI GCANNNNNTGC 2 cut(s) 596, 3410
BstAUI TGTACA 1 cut(s) 1118
BstBAI YACGTR 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 1507
BstC8I GCNNGC 8 cut(s) 268, 559, 881, 1397, 1854, 1900, 1977, 2610
BstDSI CCRYGG 4 cut(s) 1093, 1903, 1911, 2951
BstEII GGTNACC 1 cut(s) 667
BstENI CCTNNNNNAGG 2 cut(s) 1866, 2714
BstFNI CGCG 3 cut(s) 76, 399, 1344
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 2 cut(s) 152, 3418
BstMCI CGRYCG 1 cut(s) 397
BstNI CCWGG 9 cut(s) 1488, 1520, 1791, 2098, 2154, 2835, 3284, 3327, 3357
BstNSI RCATGY 4 cut(s) 412, 1856, 1902, 3601
BstPI GGTNACC 1 cut(s) 667
BstSFI CTRYAG 4 cut(s) 628, 2306, 3120, 3225
BstUI CGCG 3 cut(s) 76, 399, 1344
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 4 cut(s) 1381, 2515, 2545, 3250
BstXI CCANNNNNNTGG 1 cut(s) 2952
BstYI RGATCY 4 cut(s) 1381, 2515, 2545, 3250
Bsu15I ATCGAT 2 cut(s) 2690, 2754
Bsu36I CCTNAGG 2 cut(s) 1950, 2711
BsuI GTATCC 3 cut(s) 2734, 2841, 3086
BsuTUI ATCGAT 2 cut(s) 2690, 2754
BtgI CCRYGG 4 cut(s) 1093, 1903, 1911, 2951
BtgZI GCGATG 1 cut(s) 866
BtrI CACGTC 1 cut(s) 530
BtsI GCAGTG 2 cut(s) 617, 3234
BtsIMutI CAGTG 8 cut(s) 617, 837, 1260, 2066, 2873, 3030, 3234, 3557
BtuMI TCGCGA 1 cut(s) 399
BveI ACCTGC 2 cut(s) 1539, 3124
Cac8I GCNNGC 8 cut(s) 268, 559, 881, 1397, 1854, 1900, 1977, 2610
CaiI CAGNNNCTG 2 cut(s) 2902, 3539
CciI TCATGA 2 cut(s) 3108, 3211
CfoI GCGC 2 cut(s) 152, 3418
Cfr10I RCCGGY 3 cut(s) 129, 648, 1395
Cfr13I GGNCC 6 cut(s) 127, 285, 613, 2364, 3353, 3577
ClaI ATCGAT 2 cut(s) 2690, 2754
CseI GACGC 3 cut(s) 65, 361, 2409
Csp6I GTAC 6 cut(s) 145, 706, 798, 1119, 1539, 3291
CviQI GTAC 6 cut(s) 145, 706, 798, 1119, 1539, 3291
DinI GGCGCC 1 cut(s) 151
DraI TTTAAA 2 cut(s) 2268, 2352
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 4 cut(s) 1397, 1914, 3195, 3277
Eam1104I CTCTTC 4 cut(s) 13, 1256, 2122, 2128
EarI CTCTTC 4 cut(s) 13, 1256, 2122, 2128
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 7 cut(s) 1127, 1903, 1911, 2106, 2367, 2951, 3188
Eco147I AGGCCT 1 cut(s) 1789
Eco24I GRGCYC 3 cut(s) 813, 1471, 2305
Eco31I GGTCTC 1 cut(s) 2574
Eco32I GATATC 1 cut(s) 2103
Eco47I GGWCC 4 cut(s) 127, 285, 613, 3353
Eco57I CTGAAG 1 cut(s) 1152
Eco81I CCTNAGG 2 cut(s) 1950, 2711
Eco88I CYCGRG 3 cut(s) 1701, 2577, 2928
Eco91I GGTNACC 1 cut(s) 667
EcoNI CCTNNNNNAGG 2 cut(s) 1866, 2714
EcoO109I RGGNCCY 2 cut(s) 613, 2364
EcoO65I GGTNACC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 3101
EcoRII CCWGG 9 cut(s) 1486, 1518, 1789, 2096, 2152, 2833, 3282, 3325, 3355
EcoRV GATATC 1 cut(s) 2103
EcoT14I CCWWGG 7 cut(s) 1127, 1903, 1911, 2106, 2367, 2951, 3188
EcoT22I ATGCAT 1 cut(s) 2197
EcoT38I GRGCYC 3 cut(s) 813, 1471, 2305
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 7 cut(s) 1127, 1903, 1911, 2106, 2367, 2951, 3188
Esp3I CGTCTC 3 cut(s) 379, 1988, 2174
FalI AAGNNNNNCTT 6 cut(s) 734, 766, 1150, 1182, 2432, 2464
FaqI GGGAC 5 cut(s) 63, 146, 582, 599, 1677
FauI CCCGC 1 cut(s) 882
FauNDI CATATG 1 cut(s) 2059
FriOI GRGCYC 3 cut(s) 813, 1471, 2305
GlaI GCGC 2 cut(s) 151, 3417
GsaI CCCAGC 2 cut(s) 962, 2142
GsuI CTGGAG 2 cut(s) 1541, 3368
HaeII RGCGCY 1 cut(s) 153
HapII CCGG 5 cut(s) 130, 422, 649, 1396, 2216
HgaI GACGC 3 cut(s) 65, 361, 2409
HhaI GCGC 2 cut(s) 152, 3418
Hin1I GRCGYC 2 cut(s) 150, 372
Hin6I GCGC 2 cut(s) 150, 3416
HinP1I GCGC 2 cut(s) 150, 3416
HincII GTYRAC 3 cut(s) 468, 987, 1554
HindII GTYRAC 3 cut(s) 468, 987, 1554
HindIII AAGCTT 1 cut(s) 877
HpaI GTTAAC 2 cut(s) 468, 1554
HpaII CCGG 5 cut(s) 130, 422, 649, 1396, 2216
Hpy166II GTNNAC 8 cut(s) 157, 468, 987, 1121, 1554, 1610, 2983, 3353
Hpy8I GTNNAC 8 cut(s) 157, 468, 987, 1121, 1554, 1610, 2983, 3353
Hpy99I CGWCG 4 cut(s) 119, 365, 368, 418
HpyCH4III ACNGT 2 cut(s) 1691, 2310
HpyCH4IV ACGT 4 cut(s) 159, 481, 529, 2016
HpySE526I ACGT 4 cut(s) 159, 481, 529, 2016
Hsp92I GRCGYC 2 cut(s) 150, 372
HspAI GCGC 2 cut(s) 150, 3416
KasI GGCGCC 1 cut(s) 149
KroI GCCGGC 1 cut(s) 1395
KroNI GCCGGC 1 cut(s) 1397
KspAI GTTAAC 2 cut(s) 468, 1554
LguI GCTCTTC 1 cut(s) 2128
LmnI GCTCC 7 cut(s) 188, 330, 395, 1097, 2425, 2784, 3387
LweI GCATC 8 cut(s) 967, 1648, 2053, 2182, 2433, 2746, 2806, 3520
MaeII ACGT 4 cut(s) 159, 481, 529, 2016
MaeIII GTNAC 7 cut(s) 477, 482, 667, 1309, 1685, 2310, 2319
MbiI CCGCTC 2 cut(s) 183, 551
MfeI CAATTG 2 cut(s) 222, 2210
MflI RGATCY 4 cut(s) 1381, 2515, 2545, 3250
MhlI GDGCHC 4 cut(s) 283, 813, 1471, 2305
MlsI TGGCCA 2 cut(s) 1916, 3197
MluNI TGGCCA 2 cut(s) 1916, 3197
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 3 cut(s) 292, 1693, 3385
MmeI TCCRAC 2 cut(s) 1180, 1700
Mox20I TGGCCA 2 cut(s) 1916, 3197
Mph1103I ATGCAT 1 cut(s) 2197
MroNI GCCGGC 1 cut(s) 1395
MroXI GAANNNNTTC 4 cut(s) 750, 1359, 2720, 3049
MscI TGGCCA 2 cut(s) 1916, 3197
MslI CAYNNNNRTG 3 cut(s) 585, 2950, 3602
Msp20I TGGCCA 2 cut(s) 1916, 3197
MspA1I CMGCKG 4 cut(s) 886, 1673, 3011, 3539
MspI CCGG 5 cut(s) 130, 422, 649, 1396, 2216
MunI CAATTG 2 cut(s) 222, 2210
Mva1269I GAATGC 1 cut(s) 3486
MvaI CCWGG 9 cut(s) 1488, 1520, 1791, 2098, 2154, 2835, 3284, 3327, 3357
MvnI CGCG 3 cut(s) 76, 399, 1344
NaeI GCCGGC 1 cut(s) 1397
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 1 cut(s) 423
NcoI CCATGG 3 cut(s) 1903, 1911, 2951
NdeI CATATG 1 cut(s) 2059
NgoMIV GCCGGC 1 cut(s) 1395
NmuCI GTSAC 3 cut(s) 482, 667, 2310
NruI TCGCGA 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 2197
NspI RCATGY 4 cut(s) 412, 1856, 1902, 3601
NspV TTCGAA 1 cut(s) 1507
PaeI GCATGC 2 cut(s) 1856, 1902
PagI TCATGA 2 cut(s) 3108, 3211
PceI AGGCCT 1 cut(s) 1789
PciI ACATGT 2 cut(s) 408, 3597
PciSI GCTCTTC 1 cut(s) 2128
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 1 cut(s) 3486
PdiI GCCGGC 1 cut(s) 1397
PdmI GAANNNNTTC 4 cut(s) 750, 1359, 2720, 3049
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 2 cut(s) 2435, 3552
PfoI TCCNGGA 3 cut(s) 1518, 2096, 2833
Ple19I CGATCG 1 cut(s) 397
PleI GAGTC 3 cut(s) 292, 1693, 3384
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 3 cut(s) 292, 1693, 3384
Ppu21I YACGTR 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 613
PscI ACATGT 2 cut(s) 408, 3597
PshBI ATTAAT 1 cut(s) 2010
PsiI TTATAA 2 cut(s) 405, 966
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 9 cut(s) 1486, 1518, 1789, 2096, 2152, 2833, 3282, 3325, 3355
PspEI GGTNACC 1 cut(s) 667
PspFI CCCAGC 2 cut(s) 958, 2138
PspGI CCWGG 9 cut(s) 1486, 1518, 1789, 2096, 2152, 2833, 3282, 3325, 3355
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 6 cut(s) 127, 285, 613, 2364, 3353, 3577
PspPPI RGGWCCY 1 cut(s) 613
PstI CTGCAG 1 cut(s) 3229
PstNI CAGNNNCTG 2 cut(s) 2902, 3539
PsuI RGATCY 4 cut(s) 1381, 2515, 2545, 3250
PvuI CGATCG 1 cut(s) 397
PvuII CAGCTG 4 cut(s) 886, 1673, 3011, 3539
RruI TCGCGA 1 cut(s) 399
RsaI GTAC 6 cut(s) 146, 707, 799, 1120, 1540, 3292
RsaNI GTAC 6 cut(s) 145, 706, 798, 1119, 1539, 3291
RseI CAYNNNNRTG 3 cut(s) 585, 2950, 3602
SapI GCTCTTC 1 cut(s) 2128
Sau96I GGNCC 6 cut(s) 127, 285, 613, 2364, 3353, 3577
ScaI AGTACT 1 cut(s) 1540
SchI GAGTC 3 cut(s) 292, 1693, 3385
SduI GDGCHC 4 cut(s) 283, 813, 1471, 2305
SfaNI GCATC 8 cut(s) 967, 1648, 2053, 2182, 2433, 2746, 2806, 3520
SfcI CTRYAG 4 cut(s) 628, 2306, 3120, 3225
SfoI GGCGCC 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 1507
SinI GGWCC 4 cut(s) 127, 285, 613, 3353
SmiMI CAYNNNNRTG 3 cut(s) 585, 2950, 3602
SmlI CTYRAG 3 cut(s) 1376, 2224, 3449
SmoI CTYRAG 3 cut(s) 1376, 2224, 3449
SphI GCATGC 2 cut(s) 1856, 1902
SseBI AGGCCT 1 cut(s) 1789
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 2 cut(s) 1938, 1987
StuI AGGCCT 1 cut(s) 1789
StyI CCWWGG 7 cut(s) 1127, 1903, 1911, 2106, 2367, 2951, 3188
TaaI ACNGT 2 cut(s) 1691, 2310
TaiI ACGT 4 cut(s) 162, 484, 532, 2019
TaqI TCGA 6 cut(s) 416, 1507, 2475, 2690, 2754, 2912
TatI WGTACW 2 cut(s) 1118, 1538
TauI GCSGC 5 cut(s) 218, 372, 551, 3235, 3282
TscAI CASTG 8 cut(s) 624, 837, 1267, 2073, 2880, 3030, 3234, 3557
TseFI GTSAC 3 cut(s) 482, 667, 2310
Tsp45I GTSAC 3 cut(s) 482, 667, 2310
TspGWI ACGGA 4 cut(s) 175, 475, 2795, 3196
TspRI CASTG 8 cut(s) 624, 837, 1267, 2073, 2880, 3030, 3234, 3557
Van91I CCANNNNNTGG 2 cut(s) 2435, 3552
VpaK11BI GGWCC 4 cut(s) 127, 285, 613, 3353
VspI ATTAAT 1 cut(s) 2010
XagI CCTNNNNNAGG 2 cut(s) 1866, 2714
XapI RAATTY 7 cut(s) 859, 1053, 1297, 2671, 2838, 2940, 3101
XbaI TCTAGA 1 cut(s) 770
XceI RCATGY 4 cut(s) 412, 1856, 1902, 3601
XmaJI CCTAGG 1 cut(s) 1127
XmnI GAANNNNTTC 4 cut(s) 750, 1359, 2720, 3049
ZrmI AGTACT 1 cut(s) 1540
Zsp2I ATGCAT 1 cut(s) 2197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.