RLG00000006703

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005622
Physical Location & Seq
Forward (+)
9818723 .. 9827260
8538 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006703

Sequence Viewer

Length: 3636 bp
ATGCCCTCTTCACCATCTCCAATTCTTCTACTTCTCCATCCCTCACCTCCTTCTCCACAATCACAATTCCCTTCGCGTCCCAAAGCCTCTGCCCTCAGACCCTCACCACTACTCCTCGCCATGAAGGACCGGCCTCCTTCTTCGTACGGCGCCGTTTACGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCACCAGCCCTAATTACACCTCCCCCGCCTCAATTGCCTCCAAGAAGACTACTGCTGCTTCTCTCAGCAAAGCTCGCACCAATGGTGCTCGGACCTACTACCAGACTCAGCAGCACGAAGTTCAGGAACAGCTCCACAAGCCGAAGTTGCAGCACGATTCTGTTTACGACGACGGCGGCGTCTCCGATGATGAAGGCTCCGATCGCGATTATAACATGTCGTCGAATCCGGGTGCTTCAACCTCTGATAACCTTGATGACTGGAAACGGAAGTTAACCATGCTGTTACGTGACAACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGAGGACATAGCCGCTTTGGCAATCAGAATGGGACTTTACAGCCATCTGTATGCAAAAGTTGCTGTATTCAGTAAGGTCCCGCTGCCAAACTACAGATTTGATCTGGATGACCGGCGACCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCGTACCTCGGAGACTTCCTTTCTCAGAAGTCCAGGACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCTGAACCAGTGGCACCGAATAATGCTGCGATGGAAAAAGTTCTTTGGAGGAGAAGCTTGCAGCTGCGGGATAAGCAACACGCTTGGCAGGAATCTCGTGAAGGCAGAAAAGTTATGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAAGGATGCTTTGTTGACAGCAATTTCACGAAATCAGGTTGTTATCATATCAGGGCAAACAGGCTGTGGAAAGACCACTCAAATTCCTCAATTTATTTTAGAATCTGAGATAGAAGCTGCCCGTGGAGCTTTATGTAGTATTATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCAGAAAGAGTTGCTTCAGAGAGAGGGGAGAAGTTGGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGCATGAAAGGGAGAGACACACGCCTTCTCTTCTGCACCACTGGCATTTTGTTGAGAAGATTGCTACTTGATGGAAATTTGAAAGGTGTAACTCATGTCATTGTGGATGAGATTCACGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTTCTGGAGGATGTTCTGGAAAGTACCAGTTGCAGGTTAACTCCATACAATCAGATTGATGATTATGGACAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAAGCAGCTGATTTTAAGTGTTACAGTCCCCAGACTCGAGAATCTCTAGCCTGTTGGAATCCTGATTGTATCGGTTTTAATCTCATAGAGTATCTGTTATGCAATATCTGTGAGAATGAAAGGCCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCCCTAAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCGAGTCTTGTTGTTAGCATGCCATGGTTCAATGGCCAGTTCCGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGATGTTGTTTTTGTTATTGATTGTGGAAAGGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCTGGGCGCGTTCAACCTGGGAAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCATTTGCAGAATATCAATTGCCGGAAATCTTGAGGACACCTTTGCAGTCTCTTTGTCTGCAAATCAAAAGTTTAAAACTCGGAAGTATCTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTTGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAACGAGAATCTGACTATTTTAGGACGCTATCTAACAATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTCGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGAAAGATCTTGCGGAGGCTGCTAAATCTCAGTTTTCCCGAGACCACAGCGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCTCTCAGGAAGGAGTTCTACTCTTTGCTAAGGGATACTGATCTTATTGATGCCAACACAGCGACCTATAATGTATGGAGCTATGATGAGCATCTCGTCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATTTGCTCTGTTATGCACAATGAGAAGTCATATTCACTGAAAACTATGGAGGATGGTCAGGTGCTGCTGTACTCGAACTCTGTCAATGCTCGGGAATCTAAAATTCCATATCCATGGCTAGTTTTTAATGAGAAGATAAAGGTGAACTCTGTTTTTCTACGGGATTCAACAGCTGTATCTGATTCAGTGCTCCTCCTATTTGGTGGAAGTTTTTCAAAAGGGGATATTGATGGACATTTGAAAATGTTGGGAGGATACTTGGAATTCTTCATGAAACCTGCTGTAGCAGAAATGTATCAATGTTTAAGGGCAGAACTTGATGAGTTAATTCAAACCAAACTACGGAACCCAAGGATGGCCATCCATAAATATCATGAACTCCTTTCTGCAGTGCGGCTGCTGCTCTCAGAGGATCTATGTGAGGGTAGATTTGTATTTGGCCGCCAGGTTCGTACAACCTCAAAGCCATCTGTGGGGGCAGCTCAGCCAGGCTTGGTTTCAAGAACTGAAAGTGGACCTGGGGGTGATAATTCTAAGAGTCAGCTCCAGACATTGCTCACAAGGGCAGGATATGCTGCACCCACCTACAAAACAATGCAACTAAAGAACCTCAAGTTCCAAGCTAGTGTGGAGTTCAACGGAATGCAAATTATGGGGCAGCCTTGTAATAACAAGAAGAGTGCAGAAAAAGATGCAGCAGCTGAGGCCATTCAGTGGCTTCTGCAAAGCGCCGCCAATACACTCCGACCGGTGTGGCCCGTGACATCCGAGCAAGCTCCCTTCTCCGGTTCAGCCGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1212

Amino Acids

135.78

Weight (kDa)

8.73

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 328 - 480 2e-10 DEAD/DEAH box helicase
Helicase_C PF00271 587 - 715 1.4e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 779 - 807 6.1e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 808 - 867 5e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 934 - 1015 2.6e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1024 - 1082 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1124 - 1185 6.4e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 402, 963
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 2 cut(s) 1536, 3121
AccB1I GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
AccB7I CCANNNNNTGG 2 cut(s) 2432, 3549
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 4 cut(s) 76, 396, 1341, 2142
AclWI GGATC 5 cut(s) 1386, 1865, 2468, 2507, 3255
AcoI YGGCCR 4 cut(s) 1394, 1911, 3192, 3274
AcsI RAATTY 6 cut(s) 1050, 1294, 2668, 2835, 2937, 3098
AcuI CTGAAG 1 cut(s) 1149
AcyI GRCGYC 2 cut(s) 150, 369
AfaI GTAC 7 cut(s) 146, 704, 796, 1117, 1537, 2906, 3289
AflIII ACRYGT 1 cut(s) 405
AgeI ACCGGT 1 cut(s) 3583
AjiI CACGTC 1 cut(s) 527
AjnI CCWGG 9 cut(s) 731, 1483, 1786, 2093, 2149, 2830, 3279, 3322, 3352
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AloI GAACNNNNNNTCC 2 cut(s) 1191, 1223
Alw21I GWGCWC 2 cut(s) 280, 3027
AlwI GGATC 5 cut(s) 1386, 1865, 2468, 2507, 3255
AlwNI CAGNNNCTG 2 cut(s) 2899, 3536
Ama87I CYCGRG 3 cut(s) 1698, 2574, 2925
ApoI RAATTY 6 cut(s) 1050, 1294, 2668, 2835, 2937, 3098
AseI ATTAAT 1 cut(s) 2007
AsiGI ACCGGT 1 cut(s) 3583
Asp700I GAANNNNTTC 5 cut(s) 747, 858, 1356, 2717, 3046
AspA2I CCTAGG 1 cut(s) 1124
AspLEI GCGC 3 cut(s) 152, 2142, 3566
AspS9I GGNCC 6 cut(s) 127, 282, 610, 2361, 3350, 3591
AsuC2I CCSGG 2 cut(s) 420, 3631
AsuII TTCGAA 1 cut(s) 1504
AvaI CYCGRG 3 cut(s) 1698, 2574, 2925
AvaII GGWCC 4 cut(s) 127, 282, 610, 3350
AvrII CCTAGG 1 cut(s) 1124
AxyI CCTNAGG 1 cut(s) 1947
BaeI ACNNNNGTAYC 2 cut(s) 2994, 3027
BalI TGGCCA 2 cut(s) 1913, 3194
BanI GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BanII GRGCYC 3 cut(s) 810, 1468, 2302
BarI GAAGNNNNNNTAC 2 cut(s) 2705, 2737
BauI CACGAG 1 cut(s) 915
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 2 cut(s) 280, 3027
BbvCI CCTCAGC 2 cut(s) 686, 3537
BceAI ACGGC 3 cut(s) 137, 163, 379
BcgI CGANNNNNNTGC 4 cut(s) 896, 930, 2576, 2610
BciT130I CCWGG 9 cut(s) 733, 1485, 1788, 2095, 2151, 2832, 3281, 3324, 3354
BciVI GTATCC 3 cut(s) 2731, 2838, 3083
BcnI CCSGG 2 cut(s) 420, 3631
BfaI CTAG 9 cut(s) 768, 1125, 1430, 1709, 1799, 2175, 2295, 2954, 3459
BfmI CTRYAG 4 cut(s) 625, 2303, 3117, 3222
BfoI RGCGCY 2 cut(s) 153, 3567
BfuAI ACCTGC 2 cut(s) 1536, 3121
BfuI GTATCC 3 cut(s) 2731, 2838, 3083
BglI GCCNNNNNGGC 1 cut(s) 551
BglII AGATCT 1 cut(s) 2542
BlnI CCTAGG 1 cut(s) 1124
BlpI GCTNAGC 1 cut(s) 3318
Bme18I GGWCC 4 cut(s) 127, 282, 610, 3350
BmeT110I CYCGRG 3 cut(s) 1698, 2574, 2925
BmgBI CACGTC 1 cut(s) 527
BmgT120I GGNCC 6 cut(s) 127, 282, 610, 2361, 3350, 3591
BmrI ACTGGG 1 cut(s) 1821
BmsI GCATC 8 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
BmuI ACTGGG 1 cut(s) 1821
BpiI GAAGAC 1 cut(s) 242
BplI GAGNNNNNCTC 4 cut(s) 1430, 1462, 2708, 2740
BpmI CTGGAG 2 cut(s) 1538, 3365
Bpu10I CCTNAGC 3 cut(s) 686, 2732, 3537
Bpu1102I GCTNAGC 1 cut(s) 3318
Bpu14I TTCGAA 1 cut(s) 1504
BpuEI CTTGAG 3 cut(s) 1358, 2242, 3431
BpuMI CCSGG 2 cut(s) 420, 3631
Bsa29I ATCGAT 1 cut(s) 2687
BsaAI YACGTR 1 cut(s) 479
BsaBI GATNNNNATC 3 cut(s) 2742, 2793, 3194
BsaHI GRCGYC 2 cut(s) 150, 369
BsaI GGTCTC 1 cut(s) 2571
BsaWI WCCGGW 2 cut(s) 3583, 3620
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bse118I RCCGGY 4 cut(s) 129, 645, 1392, 3583
Bse1I ACTGG 6 cut(s) 455, 827, 1264, 1539, 1816, 1914
Bse21I CCTNAGG 1 cut(s) 1947
Bse3DI GCAATG 1 cut(s) 3386
Bse8I GATNNNNATC 3 cut(s) 2742, 2793, 3194
BseBI CCWGG 9 cut(s) 733, 1485, 1788, 2095, 2151, 2832, 3281, 3324, 3354
BseCI ATCGAT 1 cut(s) 2687
BseJI GATNNNNATC 3 cut(s) 2742, 2793, 3194
BseMI GCAATG 1 cut(s) 3386
BseNI ACTGG 6 cut(s) 455, 827, 1264, 1539, 1816, 1914
BseRI GAGGAG 4 cut(s) 104, 703, 883, 3017
BseYI CCCAGC 2 cut(s) 955, 2135
BsgI GTGCAG 4 cut(s) 1237, 2826, 3396, 3537
Bsh1236I CGCG 4 cut(s) 76, 396, 1341, 2142
Bsh1285I CGRYCG 2 cut(s) 394, 3584
BshNI GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BshTI ACCGGT 1 cut(s) 3583
BshVI ATCGAT 1 cut(s) 2687
BsiEI CGRYCG 2 cut(s) 394, 3584
BsiHKAI GWGCWC 2 cut(s) 280, 3027
BsiHKCI CYCGRG 3 cut(s) 1698, 2574, 2925
BsiSI CCGG 8 cut(s) 130, 419, 646, 1393, 2213, 3584, 3621, 3630
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
BsmBI CGTCTC 3 cut(s) 376, 1985, 2171
BsmFI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
BsmI GAATGC 1 cut(s) 3483
Bso31I GGTCTC 1 cut(s) 2571
BsoBI CYCGRG 3 cut(s) 1698, 2574, 2925
Bsp119I TTCGAA 1 cut(s) 1504
Bsp1286I GDGCHC 5 cut(s) 280, 810, 1468, 2302, 3027
Bsp1407I TGTACA 1 cut(s) 1115
Bsp1720I GCTNAGC 1 cut(s) 3318
Bsp19I CCATGG 2 cut(s) 1900, 2948
Bsp68I TCGCGA 1 cut(s) 396
BspDI ATCGAT 1 cut(s) 2687
BspFNI CGCG 4 cut(s) 76, 396, 1341, 2142
BspHI TCATGA 2 cut(s) 3105, 3208
BspMAI CTGCAG 1 cut(s) 3226
BspMI ACCTGC 2 cut(s) 1536, 3121
BspPI GGATC 5 cut(s) 1386, 1865, 2468, 2507, 3255
BspQI GCTCTTC 1 cut(s) 2125
BspT104I TTCGAA 1 cut(s) 1504
BspT107I GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BspTNI GGTCTC 1 cut(s) 2571
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 3386
BsrFI RCCGGY 4 cut(s) 129, 645, 1392, 3583
BsrGI TGTACA 1 cut(s) 1115
BsrI ACTGG 6 cut(s) 455, 827, 1264, 1539, 1816, 1914
BssAI RCCGGY 4 cut(s) 129, 645, 1392, 3583
BssNI GRCGYC 2 cut(s) 150, 369
BssSI CACGAG 1 cut(s) 915
BssT1I CCWWGG 6 cut(s) 1124, 1900, 2103, 2364, 2948, 3185
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 9 cut(s) 733, 1485, 1788, 2095, 2151, 2832, 3281, 3324, 3354
Bst4CI ACNGT 2 cut(s) 1688, 2307
Bst6I CTCTTC 5 cut(s) 13, 1253, 2119, 2125, 3506
BstACI GRCGYC 2 cut(s) 150, 369
BstAPI GCANNNNNTGC 2 cut(s) 593, 3407
BstAUI TGTACA 1 cut(s) 1115
BstBAI YACGTR 1 cut(s) 479
BstBI TTCGAA 1 cut(s) 1504
BstC8I GCNNGC 8 cut(s) 265, 878, 1394, 1851, 1897, 1974, 2607, 3609
BstDSI CCRYGG 3 cut(s) 1090, 1900, 2948
BstEII GGTNACC 1 cut(s) 664
BstENI CCTNNNNNAGG 1 cut(s) 1863
BstFNI CGCG 4 cut(s) 76, 396, 1341, 2142
BstH2I RGCGCY 2 cut(s) 153, 3567
BstHHI GCGC 3 cut(s) 152, 2142, 3566
BstMCI CGRYCG 2 cut(s) 394, 3584
BstNI CCWGG 9 cut(s) 733, 1485, 1788, 2095, 2151, 2832, 3281, 3324, 3354
BstNSI RCATGY 3 cut(s) 409, 1853, 1899
BstPI GGTNACC 1 cut(s) 664
BstSFI CTRYAG 4 cut(s) 625, 2303, 3117, 3222
BstUI CGCG 4 cut(s) 76, 396, 1341, 2142
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 4 cut(s) 1378, 2512, 2542, 3247
BstXI CCANNNNNNTGG 1 cut(s) 2949
BstYI RGATCY 4 cut(s) 1378, 2512, 2542, 3247
Bsu15I ATCGAT 1 cut(s) 2687
Bsu36I CCTNAGG 1 cut(s) 1947
BsuI GTATCC 3 cut(s) 2731, 2838, 3083
BsuTUI ATCGAT 1 cut(s) 2687
BtgI CCRYGG 3 cut(s) 1090, 1900, 2948
BtgZI GCGATG 1 cut(s) 863
BtrI CACGTC 1 cut(s) 527
BtsI GCAGTG 1 cut(s) 3231
BtsIMutI CAGTG 7 cut(s) 834, 1257, 2063, 2870, 3027, 3231, 3554
BtuMI TCGCGA 1 cut(s) 396
BveI ACCTGC 2 cut(s) 1536, 3121
Cac8I GCNNGC 8 cut(s) 265, 878, 1394, 1851, 1897, 1974, 2607, 3609
CaiI CAGNNNCTG 2 cut(s) 2899, 3536
CciI TCATGA 2 cut(s) 3105, 3208
CfoI GCGC 3 cut(s) 152, 2142, 3566
Cfr10I RCCGGY 4 cut(s) 129, 645, 1392, 3583
Cfr13I GGNCC 6 cut(s) 127, 282, 610, 2361, 3350, 3591
ClaI ATCGAT 1 cut(s) 2687
CseI GACGC 3 cut(s) 65, 358, 2406
Csp6I GTAC 7 cut(s) 145, 703, 795, 1116, 1536, 2905, 3288
CspAI ACCGGT 1 cut(s) 3583
CviQI GTAC 7 cut(s) 145, 703, 795, 1116, 1536, 2905, 3288
DinI GGCGCC 1 cut(s) 151
DraI TTTAAA 2 cut(s) 2265, 2349
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 4 cut(s) 1394, 1911, 3192, 3274
Eam1104I CTCTTC 5 cut(s) 13, 1253, 2119, 2125, 3506
EarI CTCTTC 5 cut(s) 13, 1253, 2119, 2125, 3506
Eco130I CCWWGG 6 cut(s) 1124, 1900, 2103, 2364, 2948, 3185
Eco147I AGGCCT 1 cut(s) 1786
Eco24I GRGCYC 3 cut(s) 810, 1468, 2302
Eco31I GGTCTC 1 cut(s) 2571
Eco32I GATATC 1 cut(s) 2100
Eco47I GGWCC 4 cut(s) 127, 282, 610, 3350
Eco57I CTGAAG 1 cut(s) 1149
Eco81I CCTNAGG 1 cut(s) 1947
Eco88I CYCGRG 3 cut(s) 1698, 2574, 2925
Eco91I GGTNACC 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 1863
EcoO109I RGGNCCY 2 cut(s) 610, 2361
EcoO65I GGTNACC 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 3098
EcoRII CCWGG 9 cut(s) 731, 1483, 1786, 2093, 2149, 2830, 3279, 3322, 3352
EcoRV GATATC 1 cut(s) 2100
EcoT14I CCWWGG 6 cut(s) 1124, 1900, 2103, 2364, 2948, 3185
EcoT22I ATGCAT 1 cut(s) 2194
EcoT38I GRGCYC 3 cut(s) 810, 1468, 2302
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 6 cut(s) 1124, 1900, 2103, 2364, 2948, 3185
Esp3I CGTCTC 3 cut(s) 376, 1985, 2171
FalI AAGNNNNNCTT 6 cut(s) 731, 763, 1147, 1179, 2429, 2461
FaqI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
FauI CCCGC 3 cut(s) 223, 621, 879
FauNDI CATATG 1 cut(s) 2056
FriOI GRGCYC 3 cut(s) 810, 1468, 2302
FspBI CTAG 9 cut(s) 768, 1125, 1430, 1709, 1799, 2175, 2295, 2954, 3459
GlaI GCGC 3 cut(s) 151, 2141, 3565
GsaI CCCAGC 2 cut(s) 959, 2139
GsuI CTGGAG 2 cut(s) 1538, 3365
HaeII RGCGCY 2 cut(s) 153, 3567
HapII CCGG 8 cut(s) 130, 419, 646, 1393, 2213, 3584, 3621, 3630
HgaI GACGC 3 cut(s) 65, 358, 2406
HhaI GCGC 3 cut(s) 152, 2142, 3566
Hin1I GRCGYC 2 cut(s) 150, 369
Hin6I GCGC 3 cut(s) 150, 2140, 3564
HinP1I GCGC 3 cut(s) 150, 2140, 3564
HincII GTYRAC 3 cut(s) 465, 984, 1551
HindII GTYRAC 3 cut(s) 465, 984, 1551
HindIII AAGCTT 1 cut(s) 874
HpaI GTTAAC 2 cut(s) 465, 1551
HpaII CCGG 8 cut(s) 130, 419, 646, 1393, 2213, 3584, 3621, 3630
Hpy166II GTNNAC 9 cut(s) 157, 355, 465, 984, 1118, 1551, 1607, 2980, 3350
Hpy8I GTNNAC 9 cut(s) 157, 355, 465, 984, 1118, 1551, 1607, 2980, 3350
Hpy99I CGWCG 3 cut(s) 362, 365, 415
HpyCH4III ACNGT 2 cut(s) 1688, 2307
HpyCH4IV ACGT 3 cut(s) 159, 478, 526
HpySE526I ACGT 3 cut(s) 159, 478, 526
Hsp92I GRCGYC 2 cut(s) 150, 369
HspAI GCGC 3 cut(s) 150, 2140, 3564
KasI GGCGCC 1 cut(s) 149
KroI GCCGGC 1 cut(s) 1392
KroNI GCCGGC 1 cut(s) 1394
KspAI GTTAAC 2 cut(s) 465, 1551
LguI GCTCTTC 1 cut(s) 2125
LmnI GCTCC 9 cut(s) 188, 327, 392, 1094, 2422, 2781, 3030, 3384, 3616
LweI GCATC 8 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
MaeI CTAG 9 cut(s) 768, 1125, 1430, 1709, 1799, 2175, 2295, 2954, 3459
MaeII ACGT 3 cut(s) 159, 478, 526
MaeIII GTNAC 8 cut(s) 474, 479, 664, 1306, 1682, 2307, 2316, 3595
MbiI CCGCTC 1 cut(s) 183
MfeI CAATTG 2 cut(s) 222, 2207
MflI RGATCY 4 cut(s) 1378, 2512, 2542, 3247
MhlI GDGCHC 5 cut(s) 280, 810, 1468, 2302, 3027
MlsI TGGCCA 2 cut(s) 1913, 3194
MluNI TGGCCA 2 cut(s) 1913, 3194
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 4 cut(s) 289, 1690, 1890, 3382
MmeI TCCRAC 3 cut(s) 1177, 1697, 3604
Mox20I TGGCCA 2 cut(s) 1913, 3194
Mph1103I ATGCAT 1 cut(s) 2194
MroNI GCCGGC 1 cut(s) 1392
MroXI GAANNNNTTC 5 cut(s) 747, 858, 1356, 2717, 3046
MscI TGGCCA 2 cut(s) 1913, 3194
MslI CAYNNNNRTG 2 cut(s) 582, 2947
Msp20I TGGCCA 2 cut(s) 1913, 3194
MspA1I CMGCKG 5 cut(s) 616, 883, 1670, 3008, 3536
MspI CCGG 8 cut(s) 130, 419, 646, 1393, 2213, 3584, 3621, 3630
MunI CAATTG 2 cut(s) 222, 2207
Mva1269I GAATGC 1 cut(s) 3483
MvaI CCWGG 9 cut(s) 733, 1485, 1788, 2095, 2151, 2832, 3281, 3324, 3354
MvnI CGCG 4 cut(s) 76, 396, 1341, 2142
NaeI GCCGGC 1 cut(s) 1394
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 2 cut(s) 420, 3631
NcoI CCATGG 2 cut(s) 1900, 2948
NdeI CATATG 1 cut(s) 2056
NgoMIV GCCGGC 1 cut(s) 1392
NmeAIII GCCGAG 1 cut(s) 1904
NmuCI GTSAC 4 cut(s) 479, 664, 2307, 3595
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 2194
NspI RCATGY 3 cut(s) 409, 1853, 1899
NspV TTCGAA 1 cut(s) 1504
PaeI GCATGC 2 cut(s) 1853, 1899
PaeR7I CTCGAG 1 cut(s) 1698
PagI TCATGA 2 cut(s) 3105, 3208
PceI AGGCCT 1 cut(s) 1786
PciI ACATGT 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 2125
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 1 cut(s) 3483
PdiI GCCGGC 1 cut(s) 1394
PdmI GAANNNNTTC 5 cut(s) 747, 858, 1356, 2717, 3046
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 2 cut(s) 2432, 3549
PfoI TCCNGGA 3 cut(s) 731, 2093, 2830
PinAI ACCGGT 1 cut(s) 3583
Ple19I CGATCG 1 cut(s) 394
PleI GAGTC 4 cut(s) 289, 1690, 1889, 3381
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 4 cut(s) 289, 1690, 1889, 3381
Ppu21I YACGTR 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 610
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 2007
PsiI TTATAA 2 cut(s) 402, 963
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 9 cut(s) 731, 1483, 1786, 2093, 2149, 2830, 3279, 3322, 3352
PspEI GGTNACC 1 cut(s) 664
PspFI CCCAGC 2 cut(s) 955, 2135
PspGI CCWGG 9 cut(s) 731, 1483, 1786, 2093, 2149, 2830, 3279, 3322, 3352
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 6 cut(s) 127, 282, 610, 2361, 3350, 3591
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 1 cut(s) 3226
PstNI CAGNNNCTG 2 cut(s) 2899, 3536
PsuI RGATCY 4 cut(s) 1378, 2512, 2542, 3247
PvuI CGATCG 1 cut(s) 394
PvuII CAGCTG 4 cut(s) 883, 1670, 3008, 3536
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 7 cut(s) 146, 704, 796, 1117, 1537, 2906, 3289
RsaNI GTAC 7 cut(s) 145, 703, 795, 1116, 1536, 2905, 3288
RseI CAYNNNNRTG 2 cut(s) 582, 2947
SapI GCTCTTC 1 cut(s) 2125
Sau96I GGNCC 6 cut(s) 127, 282, 610, 2361, 3350, 3591
SchI GAGTC 4 cut(s) 289, 1690, 1890, 3382
SduI GDGCHC 5 cut(s) 280, 810, 1468, 2302, 3027
SfaNI GCATC 8 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
SfcI CTRYAG 4 cut(s) 625, 2303, 3117, 3222
SfoI GGCGCC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 1698
SfuI TTCGAA 1 cut(s) 1504
SinI GGWCC 4 cut(s) 127, 282, 610, 3350
SlaI CTCGAG 1 cut(s) 1698
SmiMI CAYNNNNRTG 2 cut(s) 582, 2947
SmlI CTYRAG 4 cut(s) 1373, 1698, 2221, 3446
SmoI CTYRAG 4 cut(s) 1373, 1698, 2221, 3446
SphI GCATGC 2 cut(s) 1853, 1899
SseBI AGGCCT 1 cut(s) 1786
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 2 cut(s) 1935, 1984
SspMI CTAG 9 cut(s) 768, 1125, 1430, 1709, 1799, 2175, 2295, 2954, 3459
StuI AGGCCT 1 cut(s) 1786
StyI CCWWGG 6 cut(s) 1124, 1900, 2103, 2364, 2948, 3185
TaaI ACNGT 2 cut(s) 1688, 2307
TaiI ACGT 3 cut(s) 162, 481, 529
TaqI TCGA 6 cut(s) 413, 1504, 1699, 2472, 2687, 2909
TatI WGTACW 2 cut(s) 1115, 2904
TauI GCSGC 5 cut(s) 369, 548, 3232, 3279, 3569
TscAI CASTG 7 cut(s) 834, 1264, 2070, 2877, 3027, 3231, 3554
TseFI GTSAC 4 cut(s) 479, 664, 2307, 3595
Tsp45I GTSAC 4 cut(s) 479, 664, 2307, 3595
TspGWI ACGGA 5 cut(s) 175, 472, 2792, 3193, 3489
TspRI CASTG 7 cut(s) 834, 1264, 2070, 2877, 3027, 3231, 3554
Van91I CCANNNNNTGG 2 cut(s) 2432, 3549
VpaK11BI GGWCC 4 cut(s) 127, 282, 610, 3350
VspI ATTAAT 1 cut(s) 2007
XagI CCTNNNNNAGG 1 cut(s) 1863
XapI RAATTY 6 cut(s) 1050, 1294, 2668, 2835, 2937, 3098
XbaI TCTAGA 1 cut(s) 767
XceI RCATGY 3 cut(s) 409, 1853, 1899
XhoI CTCGAG 1 cut(s) 1698
XmaJI CCTAGG 1 cut(s) 1124
XmnI GAANNNNTTC 5 cut(s) 747, 858, 1356, 2717, 3046
XspI CTAG 9 cut(s) 768, 1125, 1430, 1709, 1799, 2175, 2295, 2954, 3459
Zsp2I ATGCAT 1 cut(s) 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.