MD16G1035100.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005622
Physical Location & Seq
Reverse (-)
2506156 .. 2514715
8560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1035100.v1.1.491

Sequence Viewer

Length: 3663 bp
ATGCCCTTTGTTTTCCTCCTCCTCCTCCCCCCATCTCCTCCGTCTCTGCAATTCCCTTGCTGGCCGTCGCCTTCGCATCCAAAACCCATTGCCTCCTCCTCCAAACCCTCACTCCTGGCGATGAAGGACCGGCCTCCTTCCTCGTACGGCTCCGTCTATGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCAGCACCCCCAATTATACCTCCCCTGCTTCAATCGGCTCCAAGCTCCGCGAAAACCAGACTGCTGCTGCTGCTCTCAACCGGAGGAGCAGCACCAATGGTGCTCTGGCGTACTACCAGACTCAGCAGCAGCAGCAGCTGCAGCAACAGGAGCAGTTTCAGAAGCCCAAGCTTCAGCACACTTCTGCTTACGACGACAGCGTTTCCGAGGAAGGCTCCGATCGCGAAGTTGAGTTCCCGTCGCATCCGACACAGAGTCCTTACTCATCTGATATTGATGAGTGGAAACGGAAATTAACCATGCTGTTACGTGACAAGGCGAAGCAAGAGTTGGTTACAAGGGAGAAAAAAGATAGACGCGACTTTGATAAAATAGCTGCTTTGGCAAGCAAAATGGGGCTTTATAGCCATCTATATTCGAAAGTTGCAGTGTTTAGTAAGGTTCCACTGCCAAACTACAGATTTGACCTGGATGATAGGCGCCCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGAGCTATCTTGGAGAGTTCCTTTCCCAGAAGTCTAGGACAAAGGAAAGCTTTCAAGATCTTTCCTTTTCTAGATCAAACAGTAGTGGCAGCATTGCTACTGACGAAGGGCTTTTTGAGCAACCAGAATCATTTGCGTCGAGTAAGGGTGTGATGGAAAAACTTCTTTGGCGGAGAAGTATGCAGCTGCGTGATAAGCAACAAGCTTGGCAGGAATCTCCTGAAGGTAGAAAAATGCTTGAACTTCGCAGAAGTCTTCCTGCTTATAAAGAGAAGGATGCATTATTGGCAGCAATTTCACGGGATCAGGTTGTTATCATATCAGGTGAAACTGGCTGTGGCAAGACTACTCAAATTCCACAGTTCATTTTAGAATCTGAGATAGAAGCTGTTCGTGGAGCTGTTTGTAGTATTATATGTACACAGCCTAGACGAATATCTGCTATGTCTGTTTCTGAAAGAGTTGCTTCAGAGAGAGGAGAGAAATTGGGTGACTCGGTTGGGTATAAGGTTCGGTTAGAAGGTATGAAGGGAAGGGACACTCGCCTTCTCTTTTGCACCACTGGCATTTTGTTGAGAAGATTACTAGTTGATAGAAATTTGAAAGGTGTAACTCATGTGATTGTGGATGAAATTCATGAACGTGGAATGAATGAAGATTTTCTGCTTATTGTACTCAAGGATCTCCTTCCTCGGCGACCAGAACTGAGGTTGATTTTGATGAGTGCAACCCTAGATTCTGAACTTTTCTCATCCTACTTTGGTAGGGCTCAGATAATTCATGTTCCAGGTTTTACATATCCAGTTCGGACTCATTTTCTGGAGGATGTTCTGGAAATTACGGGATGCAAATTAACTCCATACAATCAGATTGATGATTATGGCCAAGAAAAGATGTGGAAAATGAGCAAACAGGCACCAAGAAAGAGGAAAAGCCAAATTGTTTCTGTTGTTGAGGATGCACTTAAAGCAGCCGATTTTAAGGGGTATAGTCCACAGACTCGGGAGTCTCTAGCATGTTGGAATCCTGATAGTATCGGTTTTTATCTGATTGAGTATCTCTTGTGCAATATCTGTGAGAGTGACAAGCCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTGAAGGAAAAGTTACATGCCAATCCTCTATTAGGTGATCCAAGCCGAGTCTTGTTACTAGCATGCCATGGTTCTATGGCAAGTACTGAGCAGAGGCTAATATTTGATGAGCCTGGGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATCAATGATGTTGTTTTTGTACTTGATTGTGGAAAGGCAAAAGAGACTTCATATGACGCACTGAACAATACTCCTTGTTTGCTTCCTTCCTGGATTTCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCAGGACGTGTCCAGCCAGGGGAATGTTATCATCTCTATCCTAGATGTGTCTATGATGCATTTGCAGAGTATCAATTGCCAGAGATTTTGAGGACACCTCTGCAGTCTCTCTGTCTGCAAATAAAAAGTTTAAACCTCGGAAGCATTTCTGAGTTCTTATCTAGGGCTCTGCAGTCACCAGAATTACTCGCGGTGCAAAACGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAATGAGAATCTGACTGTTTTAGGACACTATCTTACTATGCTTCCTGTTGAGCCAAAACTTGGAAAGATGCTCTTGGTAGGTGCCATCTTCAACTGCCTGGATCCAGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCCTTACTCCATTTGACAAGAAAGATCTTGCTGAGGCTGCAAAATCTCAGTTCTCCCGGGACAATAGTGATCACCTTGCACTTGTGCGGGCCTATGAGGGCTGGAAGGTTGCTGAAAGAGACTTTGCTGGATATGATTATTGCTGGAAGAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCCCTTAGGAAGGAGTTCCTCTCTTTGCTTAGGGATACTGATCTTGTTGATACCAACACCACCACTCATAATGCATGGAGTTATGATGAGCATCTCATCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATCTGCTCTGTTGTGCACAATGAGAAGTCATTTTCGCTGAAAACCATGGAGGATGGCCAGGTGCTGCTATACTCGAACTCTGTCAATGCTAGGGATTCTAAAATTCCATATCCATGGCTGGTTTTCAATGAGAAGATAAAGGTGAACTCTGTTTTCCTACGGGATTCAACAGCTGTGTCTGACTCAGTGCTCCTCCTATTTGGTGGAAGTTTTTCGAAAGGGGATATTGATGGACACTTGAAAATGCTGGGAGGATATTTGGAATTCTTCATGAAACCTGCTATAGCAGAAATGTATCAATGCTTAAAAGAGGAACTTGATGAGTTAATTCAAATCAAACTACTGAACCCCAGGATGGACACACACAGTTATCATGAACTCCTTTCTGCAGTGCGGTTGCTGCTCTCCGAGGATCAAGGTGAAGGTAGATTTGTATTTGGCCGCCAGGTCCTTGCACCCTCAAAGTCAAAGCCATCTGTGGTGGCAGCACAGCCAGCCTTGGTTTCAAGAACTGAAAGTGGACCCGGGGGTGACAATTCCAAGAGTCAGCTCCAGACGTTGCTCACACGAGCAGGATATAATGCACCCACGTACAAAACCACGCAGCTGAAGAACAGCCAGTTCCGAGCTACAGTGGAGTTTAACGGAATGCAAATTATGGGCCACCCGTGTAACAACAAGAAGAGTGCAGAAAAAGATGCGGCAGCAGAGGCCATTCAGTGGCTGGTGAGTGGAACACAAATGGGCCATGAGTACATCAATCATATGTCAATCATGTTGAAGAAAAGCAAAAAGGAGCACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1221

Amino Acids

137.29

Weight (kDa)

8.46

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 335 - 487 2.7e-10 DEAD/DEAH box helicase
Helicase_C PF00271 594 - 722 1.2e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 786 - 814 2.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 815 - 874 4.5e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 941 - 1022 1.3e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1031 - 1089 1.7e-22 C-terminal domain in RNA helicases
dsrm PF00035 1133 - 1194 1.6e-10 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
AasI GACNNNNNNGTC 1 cut(s) 1723
Acc36I ACCTGC 1 cut(s) 3142
AccB1I GGYRCC 4 cut(s) 669, 1633, 1811, 2474
AccB7I CCANNNNNTGG 2 cut(s) 2453, 3576
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 4 cut(s) 240, 414, 549, 2345
AciI CCGC 9 cut(s) 175, 181, 238, 889, 2345, 2626, 3250, 3298, 3557
AclWI GGATC 7 cut(s) 1029, 1407, 1886, 2489, 2502, 2528, 3276
AcoI YGGCCR 4 cut(s) 62, 1600, 2911, 3295
AcsI RAATTY 6 cut(s) 1071, 1315, 1350, 2689, 2958, 3119
AcuI CTGAAG 5 cut(s) 347, 960, 1170, 1877, 3485
AcyI GRCGYC 1 cut(s) 670
AfaI GTAC 8 cut(s) 146, 302, 1138, 1392, 1939, 2046, 3449, 3611
AfiI CCNNNNNNNGG 7 cut(s) 1886, 1973, 2173, 2453, 2734, 3211, 3576
AflIII ACRYGT 1 cut(s) 2161
AhdI GACNNNNNGTC 1 cut(s) 444
AhlI ACTAGT 1 cut(s) 1303
AjiI CACGTC 1 cut(s) 2162
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
Alw21I GWGCWC 4 cut(s) 295, 2874, 3048, 3657
Alw26I GTCTC 6 cut(s) 48, 1731, 2006, 2063, 2265, 2652
Alw44I GTGCAC 1 cut(s) 2870
AlwI GGATC 7 cut(s) 1029, 1407, 1886, 2489, 2502, 2528, 3276
AlwNI CAGNNNCTG 3 cut(s) 328, 2920, 3580
Ama87I CYCGRG 3 cut(s) 1719, 2595, 3380
ApaLI GTGCAC 1 cut(s) 2870
ApoI RAATTY 6 cut(s) 1071, 1315, 1350, 2689, 2958, 3119
Asp700I GAANNNNTTC 7 cut(s) 768, 879, 1107, 1377, 2299, 2738, 3067
AspLEI GCGC 1 cut(s) 672
AspS9I GGNCC 7 cut(s) 127, 2382, 2628, 3304, 3377, 3517, 3601
AsuC2I CCSGG 4 cut(s) 2596, 2597, 3381, 3382
AsuII TTCGAA 2 cut(s) 608, 3071
AvaI CYCGRG 3 cut(s) 1719, 2595, 3380
AvaII GGWCC 3 cut(s) 127, 3304, 3377
AxyI CCTNAGG 1 cut(s) 2729
BaeGI GKGCMC 1 cut(s) 2874
BalI TGGCCA 2 cut(s) 1602, 2913
BamHI GGATCC 1 cut(s) 2494
BanI GGYRCC 4 cut(s) 669, 1633, 1811, 2474
BanII GRGCYC 2 cut(s) 1489, 2323
BauI CACGAG 1 cut(s) 3423
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 4 cut(s) 295, 2874, 3048, 3657
BbvCI CCTCAGC 2 cut(s) 707, 2571
BccI CCATC 9 cut(s) 40, 606, 865, 1967, 2486, 2903, 3080, 3205, 3337
BceAI ACGGC 2 cut(s) 49, 163
BciVI GTATCC 2 cut(s) 2752, 2859
BclI TGATCA 1 cut(s) 2608
BcnI CCSGG 4 cut(s) 2596, 2597, 3381, 3382
BcoDI GTCTC 6 cut(s) 48, 1731, 2006, 2063, 2265, 2652
BcuI ACTAGT 1 cut(s) 1303
BfmI CTRYAG 7 cut(s) 329, 646, 2255, 2324, 3138, 3243, 3486
BfoI RGCGCY 1 cut(s) 673
BfuAI ACCTGC 1 cut(s) 3142
BfuI GTATCC 2 cut(s) 2752, 2859
BglII AGATCT 2 cut(s) 775, 2563
BmcAI AGTACT 1 cut(s) 1939
Bme18I GGWCC 3 cut(s) 127, 3304, 3377
BmeRI GACNNNNNGTC 1 cut(s) 444
BmeT110I CYCGRG 3 cut(s) 1719, 2595, 3380
BmgBI CACGTC 1 cut(s) 2162
BmgT120I GGNCC 7 cut(s) 127, 2382, 2628, 3304, 3377, 3517, 3601
BmrI ACTGGG 1 cut(s) 1842
BmsI GCATC 9 cut(s) 85, 442, 985, 1553, 1666, 2200, 2451, 2824, 3544
BmuI ACTGGG 1 cut(s) 1842
BpiI GAAGAC 1 cut(s) 965
BplI GAGNNNNNCTC 6 cut(s) 389, 421, 2236, 2268, 2729, 2761
BpmI CTGGAG 2 cut(s) 1559, 3392
Bpu10I CCTNAGC 3 cut(s) 707, 2571, 2753
Bpu14I TTCGAA 2 cut(s) 608, 3071
BpuEI CTTGAG 1 cut(s) 1379
BpuMI CCSGG 4 cut(s) 2596, 2597, 3381, 3382
Bsa29I ATCGAT 1 cut(s) 2708
BsaAI YACGTR 2 cut(s) 500, 3447
BsaBI GATNNNNATC 2 cut(s) 2763, 2814
BsaHI GRCGYC 1 cut(s) 670
BsaWI WCCGGW 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 7 cut(s) 1886, 1973, 2173, 2453, 2734, 3211, 3576
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 6 cut(s) 1054, 1285, 1520, 1837, 2498, 3475
Bse21I CCTNAGG 1 cut(s) 2729
Bse3DI GCAATG 2 cut(s) 87, 810
Bse8I GATNNNNATC 2 cut(s) 2763, 2814
BseCI ATCGAT 1 cut(s) 2708
BseJI GATNNNNATC 2 cut(s) 2763, 2814
BseLI CCNNNNNNNGG 7 cut(s) 1886, 1973, 2173, 2453, 2734, 3211, 3576
BseMI GCAATG 2 cut(s) 87, 810
BseNI ACTGG 6 cut(s) 1054, 1285, 1520, 1837, 2498, 3475
BseSI GKGCMC 1 cut(s) 2874
BseYI CCCAGC 1 cut(s) 3103
BsgI GTGCAG 2 cut(s) 2847, 3564
Bsh1236I CGCG 4 cut(s) 240, 414, 549, 2345
Bsh1285I CGRYCG 1 cut(s) 412
BshNI GGYRCC 4 cut(s) 669, 1633, 1811, 2474
BshVI ATCGAT 1 cut(s) 2708
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 4 cut(s) 295, 2874, 3048, 3657
BsiHKCI CYCGRG 3 cut(s) 1719, 2595, 3380
BsiSI CCGG 4 cut(s) 130, 271, 2596, 3381
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 3 cut(s) 146, 1268, 2612
BslI CCNNNNNNNGG 7 cut(s) 1886, 1973, 2173, 2453, 2734, 3211, 3576
BsmAI GTCTC 6 cut(s) 48, 1731, 2006, 2063, 2265, 2652
BsmBI CGTCTC 2 cut(s) 48, 2006
BsmFI GGGAC 3 cut(s) 146, 1268, 2612
BsmI GAATGC 1 cut(s) 3510
BsoBI CYCGRG 3 cut(s) 1719, 2595, 3380
Bsp119I TTCGAA 2 cut(s) 608, 3071
Bsp1286I GDGCHC 6 cut(s) 295, 1489, 2323, 2874, 3048, 3657
Bsp1407I TGTACA 1 cut(s) 1136
Bsp19I CCATGG 3 cut(s) 1921, 2901, 2969
Bsp68I TCGCGA 1 cut(s) 414
BspACI CCGC 9 cut(s) 175, 181, 238, 889, 2345, 2626, 3250, 3298, 3557
BspDI ATCGAT 1 cut(s) 2708
BspFNI CGCG 4 cut(s) 240, 414, 549, 2345
BspHI TCATGA 3 cut(s) 1354, 3126, 3229
BspMAI CTGCAG 4 cut(s) 333, 2259, 2328, 3247
BspMI ACCTGC 1 cut(s) 3142
BspPI GGATC 7 cut(s) 1029, 1407, 1886, 2489, 2502, 2528, 3276
BspQI GCTCTTC 1 cut(s) 2146
BspT104I TTCGAA 2 cut(s) 608, 3071
BspT107I GGYRCC 4 cut(s) 669, 1633, 1811, 2474
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 2 cut(s) 87, 810
BsrFI RCCGGY 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 1136
BsrI ACTGG 6 cut(s) 1054, 1285, 1520, 1837, 2498, 3475
BssAI RCCGGY 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 670
BssSI CACGAG 1 cut(s) 3423
BssT1I CCWWGG 6 cut(s) 1921, 2124, 2385, 2901, 2969, 3354
Bst2BI CACGAG 1 cut(s) 3423
Bst4CI ACNGT 5 cut(s) 800, 1080, 2410, 3224, 3490
Bst6I CTCTTC 3 cut(s) 2140, 2146, 3533
BstACI GRCGYC 1 cut(s) 670
BstAPI GCANNNNNTGC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 1136
BstBAI YACGTR 2 cut(s) 500, 3447
BstBI TTCGAA 2 cut(s) 608, 3071
BstC8I GCNNGC 6 cut(s) 62, 577, 1918, 1995, 2628, 3351
BstDSI CCRYGG 3 cut(s) 1921, 2901, 2969
BstEII GGTNACC 1 cut(s) 685
BstENI CCTNNNNNAGG 2 cut(s) 1884, 2732
BstFNI CGCG 4 cut(s) 240, 414, 549, 2345
BstH2I RGCGCY 1 cut(s) 673
BstHHI GCGC 1 cut(s) 672
BstMAI GTCTC 6 cut(s) 48, 1731, 2006, 2063, 2265, 2652
BstMCI CGRYCG 1 cut(s) 412
BstNSI RCATGY 3 cut(s) 1737, 1874, 1920
BstPI GGTNACC 1 cut(s) 685
BstSFI CTRYAG 7 cut(s) 329, 646, 2255, 2324, 3138, 3243, 3486
BstSLI GKGCMC 1 cut(s) 2874
BstUI CGCG 4 cut(s) 240, 414, 549, 2345
BstV2I GAAGAC 1 cut(s) 965
BstX2I RGATCY 5 cut(s) 775, 1399, 2494, 2533, 2563
BstXI CCANNNNNNTGG 1 cut(s) 2970
BstYI RGATCY 5 cut(s) 775, 1399, 2494, 2533, 2563
Bsu15I ATCGAT 1 cut(s) 2708
Bsu36I CCTNAGG 1 cut(s) 2729
BsuI GTATCC 2 cut(s) 2752, 2859
BsuTUI ATCGAT 1 cut(s) 2708
BtgI CCRYGG 3 cut(s) 1921, 2901, 2969
BtgZI GCGATG 1 cut(s) 134
BtrI CACGTC 1 cut(s) 2162
BtsI GCAGTG 3 cut(s) 624, 635, 3252
BtsIMutI CAGTG 8 cut(s) 624, 635, 1278, 2084, 3048, 3252, 3495, 3581
BtuMI TCGCGA 1 cut(s) 414
BveI ACCTGC 1 cut(s) 3142
Cac8I GCNNGC 6 cut(s) 62, 577, 1918, 1995, 2628, 3351
CaiI CAGNNNCTG 3 cut(s) 328, 2920, 3580
CciI TCATGA 3 cut(s) 1354, 3126, 3229
CfoI GCGC 1 cut(s) 672
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 7 cut(s) 127, 2382, 2628, 3304, 3377, 3517, 3601
Cfr9I CCCGGG 2 cut(s) 2595, 3380
ClaI ATCGAT 1 cut(s) 2708
CseI GACGC 3 cut(s) 555, 843, 2090
Csp6I GTAC 8 cut(s) 145, 301, 1137, 1391, 1938, 2045, 3448, 3610
CviQI GTAC 8 cut(s) 145, 301, 1137, 1391, 1938, 2045, 3448, 3610
DinI GGCGCC 1 cut(s) 671
DraI TTTAAA 2 cut(s) 2286, 2370
DrdI GACNNNNNNGTC 1 cut(s) 1723
DriI GACNNNNNGTC 1 cut(s) 444
DseDI GACNNNNNNGTC 1 cut(s) 1723
EaeI YGGCCR 4 cut(s) 62, 1600, 2911, 3295
Eam1104I CTCTTC 3 cut(s) 2140, 2146, 3533
Eam1105I GACNNNNNGTC 1 cut(s) 444
EarI CTCTTC 3 cut(s) 2140, 2146, 3533
EciI GGCGGA 1 cut(s) 904
Eco130I CCWWGG 6 cut(s) 1921, 2124, 2385, 2901, 2969, 3354
Eco24I GRGCYC 2 cut(s) 1489, 2323
Eco47I GGWCC 3 cut(s) 127, 3304, 3377
Eco57I CTGAAG 5 cut(s) 347, 960, 1170, 1877, 3485
Eco81I CCTNAGG 1 cut(s) 2729
Eco88I CYCGRG 3 cut(s) 1719, 2595, 3380
Eco91I GGTNACC 1 cut(s) 685
EcoNI CCTNNNNNAGG 2 cut(s) 1884, 2732
EcoO109I RGGNCCY 2 cut(s) 2382, 3304
EcoO65I GGTNACC 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 3119
EcoT14I CCWWGG 6 cut(s) 1921, 2124, 2385, 2901, 2969, 3354
EcoT22I ATGCAT 3 cut(s) 1000, 2215, 2800
EcoT38I GRGCYC 2 cut(s) 1489, 2323
EgeI GGCGCC 1 cut(s) 671
EheI GGCGCC 1 cut(s) 671
ErhI CCWWGG 6 cut(s) 1921, 2124, 2385, 2901, 2969, 3354
Esp3I CGTCTC 2 cut(s) 48, 2006
FalI AAGNNNNNCTT 8 cut(s) 752, 784, 1168, 1200, 2450, 2482, 3156, 3188
FaqI GGGAC 3 cut(s) 146, 1268, 2612
FauI CCCGC 1 cut(s) 2619
FauNDI CATATG 2 cut(s) 2077, 3621
FbaI TGATCA 1 cut(s) 2608
FriOI GRGCYC 2 cut(s) 1489, 2323
GlaI GCGC 1 cut(s) 671
GsaI CCCAGC 1 cut(s) 3107
GsuI CTGGAG 2 cut(s) 1559, 3392
HaeII RGCGCY 1 cut(s) 673
HapII CCGG 4 cut(s) 130, 271, 2596, 3381
HgaI GACGC 3 cut(s) 555, 843, 2090
HhaI GCGC 1 cut(s) 672
Hin1I GRCGYC 1 cut(s) 670
Hin6I GCGC 1 cut(s) 670
HinP1I GCGC 1 cut(s) 670
HindIII AAGCTT 3 cut(s) 359, 766, 921
HpaII CCGG 4 cut(s) 130, 271, 2596, 3381
Hpy166II GTNNAC 5 cut(s) 1139, 1712, 2872, 3001, 3377
Hpy8I GTNNAC 5 cut(s) 1139, 1712, 2872, 3001, 3377
Hpy99I CGWCG 4 cut(s) 70, 386, 433, 859
HpyCH4III ACNGT 5 cut(s) 800, 1080, 2410, 3224, 3490
HpyCH4IV ACGT 5 cut(s) 499, 1360, 2161, 3413, 3446
HpySE526I ACGT 5 cut(s) 499, 1360, 2161, 3413, 3446
Hsp92I GRCGYC 1 cut(s) 670
HspAI GCGC 1 cut(s) 670
KasI GGCGCC 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 2608
LguI GCTCTTC 1 cut(s) 2146
LweI GCATC 9 cut(s) 85, 442, 985, 1553, 1666, 2200, 2451, 2824, 3544
MaeII ACGT 5 cut(s) 499, 1360, 2161, 3413, 3446
MbiI CCGCTC 1 cut(s) 183
MfeI CAATTG 1 cut(s) 2228
MflI RGATCY 5 cut(s) 775, 1399, 2494, 2533, 2563
MhlI GDGCHC 6 cut(s) 295, 1489, 2323, 2874, 3048, 3657
MlsI TGGCCA 2 cut(s) 1602, 2913
MluNI TGGCCA 2 cut(s) 1602, 2913
Mly113I GGCGCC 1 cut(s) 670
MlyI GAGTC 9 cut(s) 304, 454, 1205, 1522, 1711, 1733, 1911, 3032, 3409
MmeI TCCRAC 2 cut(s) 461, 1718
Mox20I TGGCCA 2 cut(s) 1602, 2913
Mph1103I ATGCAT 3 cut(s) 1000, 2215, 2800
MroXI GAANNNNTTC 7 cut(s) 768, 879, 1107, 1377, 2299, 2738, 3067
MscI TGGCCA 2 cut(s) 1602, 2913
MseI TTAA 9 cut(s) 485, 1571, 1683, 1698, 2285, 2369, 3161, 3182, 3498
MslI CAYNNNNRTG 2 cut(s) 1359, 2968
Msp20I TGGCCA 2 cut(s) 1602, 2913
MspA1I CMGCKG 4 cut(s) 328, 904, 3029, 3463
MspI CCGG 4 cut(s) 130, 271, 2596, 3381
MssI GTTTAAAC 1 cut(s) 2286
MunI CAATTG 1 cut(s) 2228
Mva1269I GAATGC 1 cut(s) 3510
MvnI CGCG 4 cut(s) 240, 414, 549, 2345
NarI GGCGCC 1 cut(s) 670
NciI CCSGG 4 cut(s) 2596, 2597, 3381, 3382
NcoI CCATGG 3 cut(s) 1921, 2901, 2969
NdeI CATATG 2 cut(s) 2077, 3621
NmeAIII GCCGAG 2 cut(s) 1390, 1925
NmuCI GTSAC 6 cut(s) 500, 685, 1208, 1799, 2328, 3386
NruI TCGCGA 1 cut(s) 414
NsiI ATGCAT 3 cut(s) 1000, 2215, 2800
NspI RCATGY 3 cut(s) 1737, 1874, 1920
NspV TTCGAA 2 cut(s) 608, 3071
PaeI GCATGC 1 cut(s) 1920
PagI TCATGA 3 cut(s) 1354, 3126, 3229
PciSI GCTCTTC 1 cut(s) 2146
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 3510
PdmI GAANNNNTTC 7 cut(s) 768, 879, 1107, 1377, 2299, 2738, 3067
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 2 cut(s) 2453, 3576
PfoI TCCNGGA 2 cut(s) 2114, 2851
Ple19I CGATCG 1 cut(s) 412
PleI GAGTC 9 cut(s) 304, 453, 1205, 1522, 1711, 1732, 1910, 3032, 3408
PluTI GGCGCC 1 cut(s) 673
PmeI GTTTAAAC 1 cut(s) 2286
PpsI GAGTC 9 cut(s) 304, 453, 1205, 1522, 1711, 1732, 1910, 3032, 3408
Ppu21I YACGTR 2 cut(s) 500, 3447
PpuMI RGGWCCY 1 cut(s) 3304
PsiI TTATAA 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 3304
PspEI GGTNACC 1 cut(s) 685
PspFI CCCAGC 1 cut(s) 3103
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 7 cut(s) 127, 2382, 2628, 3304, 3377, 3517, 3601
PspPPI RGGWCCY 1 cut(s) 3304
PstI CTGCAG 4 cut(s) 333, 2259, 2328, 3247
PstNI CAGNNNCTG 3 cut(s) 328, 2920, 3580
PsuI RGATCY 5 cut(s) 775, 1399, 2494, 2533, 2563
PvuI CGATCG 1 cut(s) 412
PvuII CAGCTG 4 cut(s) 328, 904, 3029, 3463
RruI TCGCGA 1 cut(s) 414
RsaI GTAC 8 cut(s) 146, 302, 1138, 1392, 1939, 2046, 3449, 3611
RsaNI GTAC 8 cut(s) 145, 301, 1137, 1391, 1938, 2045, 3448, 3610
RseI CAYNNNNRTG 2 cut(s) 1359, 2968
SapI GCTCTTC 1 cut(s) 2146
SaqAI TTAA 9 cut(s) 485, 1571, 1683, 1698, 2285, 2369, 3161, 3182, 3498
Sau96I GGNCC 7 cut(s) 127, 2382, 2628, 3304, 3377, 3517, 3601
ScaI AGTACT 1 cut(s) 1939
SchI GAGTC 9 cut(s) 304, 454, 1205, 1522, 1711, 1733, 1911, 3032, 3409
SduI GDGCHC 6 cut(s) 295, 1489, 2323, 2874, 3048, 3657
SfaNI GCATC 9 cut(s) 85, 442, 985, 1553, 1666, 2200, 2451, 2824, 3544
SfcI CTRYAG 7 cut(s) 329, 646, 2255, 2324, 3138, 3243, 3486
SfoI GGCGCC 1 cut(s) 671
SfuI TTCGAA 2 cut(s) 608, 3071
SinI GGWCC 3 cut(s) 127, 3304, 3377
SmaI CCCGGG 2 cut(s) 2597, 3382
SmiMI CAYNNNNRTG 2 cut(s) 1359, 2968
SmlI CTYRAG 1 cut(s) 1394
SmoI CTYRAG 1 cut(s) 1394
SpeI ACTAGT 1 cut(s) 1303
SphI GCATGC 1 cut(s) 1920
SsiI CCGC 9 cut(s) 175, 181, 238, 889, 2345, 2626, 3250, 3298, 3557
SspDI GGCGCC 1 cut(s) 669
SspI AATATT 2 cut(s) 1956, 2005
StyI CCWWGG 6 cut(s) 1921, 2124, 2385, 2901, 2969, 3354
TaaI ACNGT 5 cut(s) 800, 1080, 2410, 3224, 3490
TaiI ACGT 5 cut(s) 502, 1363, 2164, 3416, 3449
TaqI TCGA 5 cut(s) 608, 857, 2708, 2930, 3071
TatI WGTACW 5 cut(s) 1136, 1390, 1937, 2044, 3609
TauI GCSGC 2 cut(s) 3300, 3560
Tru1I TTAA 9 cut(s) 485, 1571, 1683, 1698, 2285, 2369, 3161, 3182, 3498
Tru9I TTAA 9 cut(s) 485, 1571, 1683, 1698, 2285, 2369, 3161, 3182, 3498
TscAI CASTG 8 cut(s) 624, 642, 1285, 2091, 3048, 3252, 3495, 3581
TseFI GTSAC 6 cut(s) 500, 685, 1208, 1799, 2328, 3386
Tsp45I GTSAC 6 cut(s) 500, 685, 1208, 1799, 2328, 3386
TspGWI ACGGA 6 cut(s) 30, 142, 175, 493, 2813, 3516
TspMI CCCGGG 2 cut(s) 2595, 3380
TspRI CASTG 8 cut(s) 624, 642, 1285, 2091, 3048, 3252, 3495, 3581
Van91I CCANNNNNTGG 2 cut(s) 2453, 3576
VneI GTGCAC 1 cut(s) 2870
VpaK11BI GGWCC 3 cut(s) 127, 3304, 3377
XagI CCTNNNNNAGG 2 cut(s) 1884, 2732
XapI RAATTY 6 cut(s) 1071, 1315, 1350, 2689, 2958, 3119
XbaI TCTAGA 1 cut(s) 788
XceI RCATGY 3 cut(s) 1737, 1874, 1920
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 3577
XmaI CCCGGG 2 cut(s) 2595, 3380
XmnI GAANNNNTTC 7 cut(s) 768, 879, 1107, 1377, 2299, 2738, 3067
ZrmI AGTACT 1 cut(s) 1939
Zsp2I ATGCAT 3 cut(s) 1000, 2215, 2800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.