Rroxscaffold_5G00375480

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005622
Physical Location & Seq
Reverse (-)
56255556 .. 56264419
8864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375480.1

Sequence Viewer

Length: 3228 bp
ATGTCGTCGAATCCGGGTGCTTCAATCTCTGATAACCTTGATGAGTGGAAACGGAAGTTAACCATGCTGTTACGTGACAACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGAGGACATAGCCGCTTTGGCAAGCAGAATGAGACTTTACAGCCATCTGTATGCAAAAGTTGCTGTATTCAGTAAGGTCCCGCTGCCAAACTACAGATTTGATCTGGATGACCGGCGACCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCGTACCTCGGAGACTTCCTTTCTCAGAAGTCCAGGACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCGGAACCAGTGGCACCGAATAATGCTGCGATGGAAAAAATTCTTTGGAGGAGAAGCTTGCAGCTGCGGGATAAGCAACACGCTTGGCAGGAATCTTGTGAAGGCAGAAAAGTTGTGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAGGGATGCTTTGTTGACAGCAATTTCACAAAATCAGGTTGTTATCATATCAGGGCAAACAGGCTGTGGAAAGACCACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGCCCGTGGAGCTGTATGTAGTATTATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCAGAAAGAGTTGCTTCAGAGAGAGGGGAGAAGTTGGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGCATGAAAGGGAGAGACACACGCCTTCTCTTCTGCACCACTGGCATTTTGTTGAGAAGATTGCTACTTGATGGAAATTTGAAAGGTGTAACTCATGTCATTGTGGATGAGATTCACGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTTCTGGAGGATGTTCTGGAAAGTACCGGTTGCAGGTTAACTCCATACAATCAGATTGATGATTATGGACAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAAGCAGCTGATTTTAAGGGTTACAGTCCCCAGACTCGGGAATCTCTAGCCTGTTGGAATCCTGATTGTATCGGTTTTAATCTCATAGAGTATCTGTTATGCAATATCTGTGAGAATGAAAGGTCTGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTAAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCAAGTCTTGTTGTTAGCATGCCATGGTTCCATGGCCAGTTCCGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGACGTTGTTTTTGTTATTGATTGTGGAAAGGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGGTGGGCGCGTTCAACCTGGGGAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCATTTGCAGAATATCAATTGCCGGAAATCTTGAGGACACCTTTGCAGTCTCTTTGTCTGCAAATCAAAAGTTTAAAACTCGGAAGTATCTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTCGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAACGAGAATCTGACTATTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTCGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGAAAGATCTTGCGGAGGCTGCTAAATCTCAGTTTTCCCGAGACCACAGCGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGATTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCTCTCAGGAAGGAGTTCTACTCTTTGCTAAGGGATACTGATCTTATCGATGCCAACACAGCTACCTATAATGTATGGAGCTATGATGAGCATCTCGTCCGTGCGGTTATTTGCTATGGCCTGTATCCTGGAATTTGCTCTGTTATGCACAATGAGAAGTCATATTCACTGAAAACTATGGAGGATGGTCAGGTGCTGCTATACTCGAACTCTGTCAATGCTCGGGAATCCAAAATTCCATATCCATGGCTAGTTTTTAATGAGAAGATAAAGGTGAACTCTGTTTTTCTACGGGATTCAACTGCTGTATCTGACTCAGTGCTTCTCCTATTTGGTGGAAGTTTTTCAAAAGGGGATATTGATGGACACTTGAAAATGTTGGGAGGATACTTGGAATTCTTCATGAAACCTGCTGTAGCAGAAATGTATCAATGTTTAAGGGCAGAACTTGATGAGTTAATTCAAACCAAACTACGGAACCCAAGGATGGCCATCCACAAATATCATGAACTCCTTTCTGCAGTGCGGCTGCTGCTCTCGGAGGATCTATGTGAGGGTAGATTTGTATTTGGCCGCCAGGTTCGTACAACCTCAAAGCCATCCGCGGGGGCAGCTCAGCCAGGCTTGGTTTCAAGAACTGAAAGTGGACCTGGGGGTGATAATTCTAAGAGTCAGCTCCAGACATTGCTCACAAGGGCAGGATATGCTGCGCCCACCTACAAAACAATGCAACTAAAGAACCTCAAGTTCCAAGCTAGTGTGGAGTTCAATGGAATGCAAATTATGGGGCAGCCTTGTAATAACAAGAAGAGTGCAGAAAAAGATGCAGCAGCTGAGGCCATTCAGTGGCTTGTGAGTGGAACAAAAATGGGCCATGACCACATCAATCACATGTCAATGATGTTGAAGAAAAGCAAAAAAGGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1075

Amino Acids

120.97

Weight (kDa)

8.58

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 193 - 345 5.7e-11 DEAD/DEAH box helicase
Helicase_C PF00271 452 - 580 6.4e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 644 - 672 5.3e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 673 - 732 4.4e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 799 - 880 2.3e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 889 - 947 2.3e-23 C-terminal domain in RNA helicases
dsrm PF00035 989 - 1050 5.5e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
Acc36I ACCTGC 2 cut(s) 1131, 2716
AccB1I GGYRCC 4 cut(s) 427, 1207, 1385, 2048
AccB7I CCANNNNNTGG 2 cut(s) 2027, 3144
AccII CGCG 4 cut(s) 936, 1737, 1919, 2903
AclWI GGATC 5 cut(s) 981, 1460, 2063, 2102, 2850
AcoI YGGCCR 4 cut(s) 989, 1506, 2787, 2869
AcsI RAATTY 7 cut(s) 453, 645, 889, 2263, 2430, 2532, 2693
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 5 cut(s) 299, 391, 712, 1132, 2884
AflIII ACRYGT 1 cut(s) 3189
AgeI ACCGGT 1 cut(s) 1133
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 8 cut(s) 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AloI GAACNNNNNNTCC 2 cut(s) 786, 818
Alw26I GTCTC 9 cut(s) 154, 300, 551, 822, 1580, 1637, 1766, 1839, 2166
AlwI GGATC 5 cut(s) 981, 1460, 2063, 2102, 2850
AlwNI CAGNNNCTG 2 cut(s) 2494, 3131
Ama87I CYCGRG 3 cut(s) 1293, 2169, 2520
AoxI GGCC 9 cut(s) 989, 1506, 1956, 2092, 2416, 2787, 2869, 3135, 3169
ApoI RAATTY 7 cut(s) 453, 645, 889, 2263, 2430, 2532, 2693
ArsI GACNNNNNNTTYG 2 cut(s) 1598, 1630
AseI ATTAAT 1 cut(s) 1602
AsiGI ACCGGT 1 cut(s) 1133
Asp700I GAANNNNTTC 5 cut(s) 342, 453, 951, 2312, 2641
AspA2I CCTAGG 1 cut(s) 719
AspLEI GCGC 2 cut(s) 1737, 3010
AspS9I GGNCC 4 cut(s) 205, 1956, 2945, 3169
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 8 cut(s) 271, 794, 1475, 1896, 1933, 2177, 2584, 2966
AsuII TTCGAA 1 cut(s) 1099
AvaI CYCGRG 3 cut(s) 1293, 2169, 2520
AvaII GGWCC 2 cut(s) 205, 2945
AvrII CCTAGG 1 cut(s) 719
AxyI CCTNAGG 1 cut(s) 1542
BaeI ACNNNNGTAYC 2 cut(s) 2589, 2622
BalI TGGCCA 2 cut(s) 1508, 2789
BanI GGYRCC 4 cut(s) 427, 1207, 1385, 2048
BanII GRGCYC 3 cut(s) 405, 1063, 1897
BarI GAAGNNNNNNTAC 2 cut(s) 2300, 2332
BbvCI CCTCAGC 2 cut(s) 281, 3132
BcgI CGANNNNNNTGC 2 cut(s) 2171, 2205
BciT130I CCWGG 8 cut(s) 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
BciVI GTATCC 3 cut(s) 2326, 2433, 2678
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 9 cut(s) 154, 300, 551, 822, 1580, 1637, 1766, 1839, 2166
BfaI CTAG 9 cut(s) 363, 720, 1025, 1304, 1394, 1770, 1890, 2549, 3054
BfmI CTRYAG 4 cut(s) 220, 1898, 2712, 2817
BfuAI ACCTGC 2 cut(s) 1131, 2716
BfuI GTATCC 3 cut(s) 2326, 2433, 2678
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 1 cut(s) 2137
BlnI CCTAGG 1 cut(s) 719
BlpI GCTNAGC 1 cut(s) 2913
Bme1390I CCNGG 9 cut(s) 15, 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
Bme18I GGWCC 2 cut(s) 205, 2945
BmeT110I CYCGRG 3 cut(s) 1293, 2169, 2520
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 4 cut(s) 205, 1956, 2945, 3169
BmrFI CCNGG 9 cut(s) 15, 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
BmrI ACTGGG 1 cut(s) 1416
BmsI GCATC 8 cut(s) 559, 1240, 1645, 1774, 2025, 2338, 2398, 3112
BmuI ACTGGG 1 cut(s) 1416
BplI GAGNNNNNCTC 4 cut(s) 1025, 1057, 2303, 2335
BpmI CTGGAG 2 cut(s) 1133, 2960
Bpu10I CCTNAGC 3 cut(s) 281, 2327, 3132
Bpu1102I GCTNAGC 1 cut(s) 2913
Bpu14I TTCGAA 1 cut(s) 1099
BpuEI CTTGAG 3 cut(s) 953, 1837, 3026
BpuMI CCSGG 1 cut(s) 15
Bsa29I ATCGAT 2 cut(s) 2282, 2346
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 3 cut(s) 2337, 2388, 2789
BsaI GGTCTC 1 cut(s) 2166
BsaWI WCCGGW 2 cut(s) 415, 1133
Bse118I RCCGGY 3 cut(s) 240, 987, 1133
Bse1I ACTGG 4 cut(s) 422, 859, 1411, 1509
Bse21I CCTNAGG 1 cut(s) 1542
Bse3DI GCAATG 1 cut(s) 2981
Bse8I GATNNNNATC 3 cut(s) 2337, 2388, 2789
BseBI CCWGG 8 cut(s) 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
BseCI ATCGAT 2 cut(s) 2282, 2346
BseJI GATNNNNATC 3 cut(s) 2337, 2388, 2789
BseMI GCAATG 1 cut(s) 2981
BseNI ACTGG 4 cut(s) 422, 859, 1411, 1509
BseRI GAGGAG 2 cut(s) 298, 478
BseYI CCCAGC 1 cut(s) 550
BsgI GTGCAG 2 cut(s) 832, 3132
Bsh1236I CGCG 4 cut(s) 936, 1737, 1919, 2903
BshFI GGCC 9 cut(s) 991, 1508, 1958, 2094, 2418, 2789, 2871, 3137, 3171
BshNI GGYRCC 4 cut(s) 427, 1207, 1385, 2048
BshTI ACCGGT 1 cut(s) 1133
BshVI ATCGAT 2 cut(s) 2282, 2346
BsiHKCI CYCGRG 3 cut(s) 1293, 2169, 2520
BsiSI CCGG 6 cut(s) 14, 241, 416, 988, 1134, 1808
BslFI GGGAC 2 cut(s) 191, 1269
BsmAI GTCTC 9 cut(s) 154, 300, 551, 822, 1580, 1637, 1766, 1839, 2166
BsmBI CGTCTC 2 cut(s) 1580, 1766
BsmFI GGGAC 2 cut(s) 191, 1269
BsmI GAATGC 1 cut(s) 3078
BsnI GGCC 9 cut(s) 991, 1508, 1958, 2094, 2418, 2789, 2871, 3137, 3171
Bso31I GGTCTC 1 cut(s) 2166
BsoBI CYCGRG 3 cut(s) 1293, 2169, 2520
Bsp119I TTCGAA 1 cut(s) 1099
Bsp1286I GDGCHC 3 cut(s) 405, 1063, 1897
Bsp1407I TGTACA 1 cut(s) 710
Bsp1720I GCTNAGC 1 cut(s) 2913
Bsp19I CCATGG 3 cut(s) 1495, 1503, 2543
BspANI GGCC 9 cut(s) 991, 1508, 1958, 2094, 2418, 2789, 2871, 3137, 3171
BspDI ATCGAT 2 cut(s) 2282, 2346
BspFNI CGCG 4 cut(s) 936, 1737, 1919, 2903
BspHI TCATGA 2 cut(s) 2700, 2803
BspMAI CTGCAG 1 cut(s) 2821
BspMI ACCTGC 2 cut(s) 1131, 2716
BspPI GGATC 5 cut(s) 981, 1460, 2063, 2102, 2850
BspT104I TTCGAA 1 cut(s) 1099
BspT107I GGYRCC 4 cut(s) 427, 1207, 1385, 2048
BspTNI GGTCTC 1 cut(s) 2166
BsrDI GCAATG 1 cut(s) 2981
BsrFI RCCGGY 3 cut(s) 240, 987, 1133
BsrGI TGTACA 1 cut(s) 710
BsrI ACTGG 4 cut(s) 422, 859, 1411, 1509
BssAI RCCGGY 3 cut(s) 240, 987, 1133
BssT1I CCWWGG 7 cut(s) 719, 1495, 1503, 1698, 1959, 2543, 2780
Bst2UI CCWGG 8 cut(s) 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
Bst4CI ACNGT 2 cut(s) 1283, 1902
Bst6I CTCTTC 4 cut(s) 848, 1714, 1720, 3101
BstAPI GCANNNNNTGC 2 cut(s) 188, 3002
BstAUI TGTACA 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 1099
BstC8I GCNNGC 7 cut(s) 151, 473, 989, 1446, 1492, 1569, 2202
BstDSI CCRYGG 5 cut(s) 685, 1495, 1503, 2543, 2901
BstEII GGTNACC 1 cut(s) 259
BstENI CCTNNNNNAGG 1 cut(s) 1458
BstFNI CGCG 4 cut(s) 936, 1737, 1919, 2903
BstHHI GCGC 2 cut(s) 1737, 3010
BstMAI GTCTC 9 cut(s) 154, 300, 551, 822, 1580, 1637, 1766, 1839, 2166
BstNI CCWGG 8 cut(s) 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
BstNSI RCATGY 3 cut(s) 1448, 1494, 3193
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 9 cut(s) 13, 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
BstSFI CTRYAG 4 cut(s) 220, 1898, 2712, 2817
BstUI CGCG 4 cut(s) 936, 1737, 1919, 2903
BstX2I RGATCY 4 cut(s) 973, 2107, 2137, 2842
BstXI CCANNNNNNTGG 1 cut(s) 2544
BstYI RGATCY 4 cut(s) 973, 2107, 2137, 2842
Bsu15I ATCGAT 2 cut(s) 2282, 2346
Bsu36I CCTNAGG 1 cut(s) 1542
BsuI GTATCC 3 cut(s) 2326, 2433, 2678
BsuRI GGCC 9 cut(s) 991, 1508, 1958, 2094, 2418, 2789, 2871, 3137, 3171
BsuTUI ATCGAT 2 cut(s) 2282, 2346
BtgI CCRYGG 5 cut(s) 685, 1495, 1503, 2543, 2901
BtgZI GCGATG 1 cut(s) 458
BtrI CACGTC 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 2826
BtsIMutI CAGTG 7 cut(s) 429, 852, 1658, 2465, 2622, 2826, 3149
BveI ACCTGC 2 cut(s) 1131, 2716
Cac8I GCNNGC 7 cut(s) 151, 473, 989, 1446, 1492, 1569, 2202
CaiI CAGNNNCTG 2 cut(s) 2494, 3131
CciI TCATGA 2 cut(s) 2700, 2803
CfoI GCGC 2 cut(s) 1737, 3010
Cfr10I RCCGGY 3 cut(s) 240, 987, 1133
Cfr13I GGNCC 4 cut(s) 205, 1956, 2945, 3169
Cfr42I CCGCGG 1 cut(s) 2904
ClaI ATCGAT 2 cut(s) 2282, 2346
CseI GACGC 1 cut(s) 2001
Csp6I GTAC 5 cut(s) 298, 390, 711, 1131, 2883
CspAI ACCGGT 1 cut(s) 1133
CviQI GTAC 5 cut(s) 298, 390, 711, 1131, 2883
DraI TTTAAA 2 cut(s) 1860, 1944
EaeI YGGCCR 4 cut(s) 989, 1506, 2787, 2869
Eam1104I CTCTTC 4 cut(s) 848, 1714, 1720, 3101
EarI CTCTTC 4 cut(s) 848, 1714, 1720, 3101
Eco130I CCWWGG 7 cut(s) 719, 1495, 1503, 1698, 1959, 2543, 2780
Eco24I GRGCYC 3 cut(s) 405, 1063, 1897
Eco31I GGTCTC 1 cut(s) 2166
Eco32I GATATC 1 cut(s) 1695
Eco47I GGWCC 2 cut(s) 205, 2945
Eco57I CTGAAG 1 cut(s) 744
Eco81I CCTNAGG 1 cut(s) 1542
Eco88I CYCGRG 3 cut(s) 1293, 2169, 2520
Eco91I GGTNACC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 1458
EcoO109I RGGNCCY 2 cut(s) 205, 1956
EcoO65I GGTNACC 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 2693
EcoRII CCWGG 8 cut(s) 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
EcoRV GATATC 1 cut(s) 1695
EcoT14I CCWWGG 7 cut(s) 719, 1495, 1503, 1698, 1959, 2543, 2780
EcoT22I ATGCAT 1 cut(s) 1789
EcoT38I GRGCYC 3 cut(s) 405, 1063, 1897
ErhI CCWWGG 7 cut(s) 719, 1495, 1503, 1698, 1959, 2543, 2780
Esp3I CGTCTC 2 cut(s) 1580, 1766
FalI AAGNNNNNCTT 6 cut(s) 326, 358, 742, 774, 2024, 2056
FaqI GGGAC 2 cut(s) 191, 1269
FauI CCCGC 3 cut(s) 216, 474, 2896
FauNDI CATATG 1 cut(s) 1651
FriOI GRGCYC 3 cut(s) 405, 1063, 1897
FspBI CTAG 9 cut(s) 363, 720, 1025, 1304, 1394, 1770, 1890, 2549, 3054
GlaI GCGC 2 cut(s) 1736, 3009
GsaI CCCAGC 1 cut(s) 554
GsuI CTGGAG 2 cut(s) 1133, 2960
HaeIII GGCC 9 cut(s) 991, 1508, 1958, 2094, 2418, 2789, 2871, 3137, 3171
HapII CCGG 6 cut(s) 14, 241, 416, 988, 1134, 1808
HgaI GACGC 1 cut(s) 2001
HhaI GCGC 2 cut(s) 1737, 3010
Hin6I GCGC 2 cut(s) 1735, 3008
HinP1I GCGC 2 cut(s) 1735, 3008
HincII GTYRAC 3 cut(s) 60, 579, 1146
HindII GTYRAC 3 cut(s) 60, 579, 1146
HindIII AAGCTT 1 cut(s) 469
HpaI GTTAAC 2 cut(s) 60, 1146
HpaII CCGG 6 cut(s) 14, 241, 416, 988, 1134, 1808
HphI GGTGA 8 cut(s) 271, 794, 1475, 1896, 1933, 2177, 2584, 2966
Hpy166II GTNNAC 7 cut(s) 60, 579, 713, 1146, 1202, 2575, 2945
Hpy8I GTNNAC 7 cut(s) 60, 579, 713, 1146, 1202, 2575, 2945
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 392, 509, 848, 989, 1695, 2212, 2302
HpyCH4III ACNGT 2 cut(s) 1283, 1902
HpyCH4IV ACGT 3 cut(s) 73, 121, 1608
HpySE526I ACGT 3 cut(s) 73, 121, 1608
HspAI GCGC 2 cut(s) 1735, 3008
KroI GCCGGC 1 cut(s) 987
KroNI GCCGGC 1 cut(s) 989
KspAI GTTAAC 2 cut(s) 60, 1146
KspI CCGCGG 1 cut(s) 2904
LmnI GCTCC 4 cut(s) 689, 2017, 2376, 2979
LweI GCATC 8 cut(s) 559, 1240, 1645, 1774, 2025, 2338, 2398, 3112
MaeI CTAG 9 cut(s) 363, 720, 1025, 1304, 1394, 1770, 1890, 2549, 3054
MaeII ACGT 3 cut(s) 73, 121, 1608
MaeIII GTNAC 7 cut(s) 69, 74, 259, 901, 1277, 1902, 1911
MfeI CAATTG 1 cut(s) 1802
MflI RGATCY 4 cut(s) 973, 2107, 2137, 2842
MhlI GDGCHC 3 cut(s) 405, 1063, 1897
MlsI TGGCCA 2 cut(s) 1508, 2789
MluNI TGGCCA 2 cut(s) 1508, 2789
MlyI GAGTC 3 cut(s) 1285, 2606, 2977
MmeI TCCRAC 2 cut(s) 772, 1292
Mox20I TGGCCA 2 cut(s) 1508, 2789
Mph1103I ATGCAT 1 cut(s) 1789
MroNI GCCGGC 1 cut(s) 987
MroXI GAANNNNTTC 5 cut(s) 342, 453, 951, 2312, 2641
MscI TGGCCA 2 cut(s) 1508, 2789
MslI CAYNNNNRTG 3 cut(s) 177, 2542, 3194
Msp20I TGGCCA 2 cut(s) 1508, 2789
MspA1I CMGCKG 5 cut(s) 211, 478, 1265, 2903, 3131
MspI CCGG 6 cut(s) 14, 241, 416, 988, 1134, 1808
MspR9I CCNGG 9 cut(s) 15, 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
MunI CAATTG 1 cut(s) 1802
Mva1269I GAATGC 1 cut(s) 3078
MvaI CCWGG 8 cut(s) 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
MvnI CGCG 4 cut(s) 936, 1737, 1919, 2903
NaeI GCCGGC 1 cut(s) 989
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 3 cut(s) 1495, 1503, 2543
NdeI CATATG 1 cut(s) 1651
NgoMIV GCCGGC 1 cut(s) 987
NmuCI GTSAC 3 cut(s) 74, 259, 1902
NsiI ATGCAT 1 cut(s) 1789
NspI RCATGY 3 cut(s) 1448, 1494, 3193
NspV TTCGAA 1 cut(s) 1099
PaeI GCATGC 2 cut(s) 1448, 1494
PagI TCATGA 2 cut(s) 2700, 2803
PciI ACATGT 1 cut(s) 3189
PctI GAATGC 1 cut(s) 3078
PdiI GCCGGC 1 cut(s) 989
PdmI GAANNNNTTC 5 cut(s) 342, 453, 951, 2312, 2641
PflMI CCANNNNNTGG 2 cut(s) 2027, 3144
PfoI TCCNGGA 3 cut(s) 326, 1688, 2425
PinAI ACCGGT 1 cut(s) 1133
PleI GAGTC 3 cut(s) 1285, 2606, 2976
PpsI GAGTC 3 cut(s) 1285, 2606, 2976
Ppu21I YACGTR 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 3189
PshBI ATTAAT 1 cut(s) 1602
PsiI TTATAA 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 8 cut(s) 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
PspEI GGTNACC 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 8 cut(s) 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
PspPI GGNCC 4 cut(s) 205, 1956, 2945, 3169
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 1 cut(s) 2821
PstNI CAGNNNCTG 2 cut(s) 2494, 3131
PsuI RGATCY 4 cut(s) 973, 2107, 2137, 2842
PvuII CAGCTG 3 cut(s) 478, 1265, 3131
RsaI GTAC 5 cut(s) 299, 391, 712, 1132, 2884
RsaNI GTAC 5 cut(s) 298, 390, 711, 1131, 2883
RseI CAYNNNNRTG 3 cut(s) 177, 2542, 3194
SacII CCGCGG 1 cut(s) 2904
Sau96I GGNCC 4 cut(s) 205, 1956, 2945, 3169
SchI GAGTC 3 cut(s) 1285, 2606, 2977
ScrFI CCNGG 9 cut(s) 15, 328, 1080, 1690, 1746, 2427, 2876, 2919, 2949
SduI GDGCHC 3 cut(s) 405, 1063, 1897
SfaNI GCATC 8 cut(s) 559, 1240, 1645, 1774, 2025, 2338, 2398, 3112
SfcI CTRYAG 4 cut(s) 220, 1898, 2712, 2817
Sfr303I CCGCGG 1 cut(s) 2904
SfuI TTCGAA 1 cut(s) 1099
SgrBI CCGCGG 1 cut(s) 2904
SinI GGWCC 2 cut(s) 205, 2945
SmiMI CAYNNNNRTG 3 cut(s) 177, 2542, 3194
SmlI CTYRAG 3 cut(s) 968, 1816, 3041
SmoI CTYRAG 3 cut(s) 968, 1816, 3041
SphI GCATGC 2 cut(s) 1448, 1494
SspI AATATT 2 cut(s) 1530, 1579
SspMI CTAG 9 cut(s) 363, 720, 1025, 1304, 1394, 1770, 1890, 2549, 3054
StyD4I CCNGG 9 cut(s) 13, 326, 1078, 1688, 1744, 2425, 2874, 2917, 2947
StyI CCWWGG 7 cut(s) 719, 1495, 1503, 1698, 1959, 2543, 2780
TaaI ACNGT 2 cut(s) 1283, 1902
TaiI ACGT 3 cut(s) 76, 124, 1611
TaqI TCGA 6 cut(s) 8, 1099, 2067, 2282, 2346, 2504
TatI WGTACW 1 cut(s) 710
TauI GCSGC 3 cut(s) 143, 2827, 2874
TscAI CASTG 7 cut(s) 429, 859, 1665, 2472, 2622, 2826, 3149
TseFI GTSAC 3 cut(s) 74, 259, 1902
Tsp45I GTSAC 3 cut(s) 74, 259, 1902
TspGWI ACGGA 3 cut(s) 67, 2387, 2788
TspRI CASTG 7 cut(s) 429, 859, 1665, 2472, 2622, 2826, 3149
Van91I CCANNNNNTGG 2 cut(s) 2027, 3144
VpaK11BI GGWCC 2 cut(s) 205, 2945
VspI ATTAAT 1 cut(s) 1602
XagI CCTNNNNNAGG 1 cut(s) 1458
XapI RAATTY 7 cut(s) 453, 645, 889, 2263, 2430, 2532, 2693
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 3 cut(s) 1448, 1494, 3193
XmaJI CCTAGG 1 cut(s) 719
XmnI GAANNNNTTC 5 cut(s) 342, 453, 951, 2312, 2641
XspI CTAG 9 cut(s) 363, 720, 1025, 1304, 1394, 1770, 1890, 2549, 3054
Zsp2I ATGCAT 1 cut(s) 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.