FvH4_4g26440

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005622
Physical Location & Seq
Reverse (-)
27818751 .. 27828124
9374 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26440.t2

Sequence Viewer

Length: 3642 bp
ATGCTCTCTCCACAGTCTCCGATTCTTCTTCCTCCTCACCTCTCATCTCCTTCTCCACATTCACAATTCCCTTTTTGCCCCTCCGCCTCTGCCCTGAGACCCTCACCGTCACCGCCGTCGTCTCTCCCGCTCGCCATGAAGGACCGGCCTCCTCCTTCTCCCTACGGCGCCGTTTACGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCACCTCCCCTAATTACACCTCCGCCGCCTCAATTGCCTCCATGAAGAAGACTACTGCCCCCGCTTCCCTCAGCAAGGCTCGCTCCAATGGCACTCGGGCCTACTACCAGACGCTGCCGCAGGAACAGCTCCGCAAGCCGGAGTTGCAGCGCGATTCTGACGGCGCCGGCGGCGTCTCCGATGATGTCTCCGATCGCGATTATAACATGTCGTCGAATCCGGTTGCTTCAGTCTCTGATAACATTGATGAGTGGAAACGGAAGTTAACCATGCTGTTACGTGACGACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGATGACATAGCCGCTTTGGCAAGCAGAATGGGACTTTACAGCCATCTGTATGCAAAGGTTGCTGTATTCAGTAAGGTACCCCTGCCAAACTACAGATTTGATCTCGATGACCGGCGACCTCAAAGAGAGGTGAGCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCCTACCTTGGAGACTTCCTTTCTCAGAAGTCTAGAACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCTGAACCAGTGGTATCTAATAATGCTGTGATGGAAAAAGTTCTTTGGAGGAGAAGCTTGCAGCTGCGGGAAAAGGAACAAGCTTGGCAGGAATCTCGTGAAGGCAGAAAAGTTATGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAAGGATGCTTTGTTGACAGCAATTTCACGAAATCAGGTTGTTATCATATCAGGGGAAACAGGCTGCGGAAAGACCACCCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTTCCCGTGGAGCTGTTTGTAGTATCATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCTGAAAGAGTTGCTTCTGAGAGAGGGGAGAAGCTCGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGTATGAAAGGGAAAGACACACGGCTTCTCTTCTGTACCACTGGCATTTTGTTGAGAAGATTGCTAGTCGATGGAAGTTTGAAAGGTGTAACTCATGTCATTGTGGATGAGATTCATGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATGCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTTCTGGAAGATGTTCTGGAAAGTACGGGTTGCAGGCTAACTCCATACAATCAGATTGATGATTATGGCCAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAGGCAGCTAACTTTAAAGGTTACAGTCCCCAGACTCGGGAATCTCTAGCCTGTTGGAATCCTGATTGTATCGGTTTTAATCTCATAGAGTATCTCTTATGCAATATCTGTGAGAATGAAAGGCCTGGTGCCATTCTAGTCTTTATGACTGGGTGGGATGACATAAATTCTCTGAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCGAGTCTTGTTGCTAGCATGCCATGGTTCCATGGCCAGTTCTGAGCAGAGGTTAATATTTGATGAGCCTGAAGATGGAGTCAGAAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGATGTTGTTTTTGTTGTTGATTGTGGAAAAGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCGAAGGTTTCTGCTCAACAGAGAAGAGGAAGAGCTGGGCGCGTTCAACCTGGGGAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCGTTTGCAGAATATCAATTGCCGGAAATCTTGAGGACACCTCTGCAGTCCCTTTGTCTGCAAATCAAAAGTTTAAAATTGGGAAGTATTTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTTGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAAAACGAGAATCTGACTATTTTAGGACGCTATCTAACTATGCTTCCAGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTAGGTTGTATCTTCAACTGCCTGGATCCTGTTTTGACTGTTGTTTCCGGCCTTAGTGTGAGAGATCCTTTCTTAACACCGTTTGACAAGAAAGATCTTGCAGAGGCTGCAAAATCTCAGTTTTCCCGAGACCACAGTGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAAGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCAATGAAAGCTATTGATTCTCTCAGGAAAGAGTTCTTGTCTTTGCTAAGGGATACTGATCTTATCGATGCCAACACAGCTACCTATAATGTATGGAGCTATGATGTTCATCTTGTCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATTTGCTCTGTTATGCACAATGAGAAGTCATTTTCACTGAAAACTATGGAGGATGGTCAGGTGCTGCTATACTCGAACTCTGTCAATGCTCGGGAATCTAAAATTCCATATCCCTGGCTGGTTTTCAATGAGAAGATAAAGGTGAACTCCGTTTTTCTACGGGATTCAACAGCTGTGTCTGATTCAGTGCTCCTTCTATTTGGTGGAAGTTTTTCAAAAGGGCATATTGATGGACACTTGAAAATGTTGGGTGGATACTTAGAATTTTTCATGAAACCTGCTGTAGCAGAAATGTATCAATGTATAAGGACGGAACTTGATGAGTTAATTCAGACCAAACTACGGAACCCAAGGATGGCCATCCACAAATATCATGAACTCCTTTCTGCAGTGCGGTTGCTGCTCTCCGAGGATCAAGGTGAGGGTAGATTTGTATTTGGCCGCCAGGTCCATACATCCTTAAAAGCATCTGTGGGGGTAGCTCAGCCAGGCTTGGTTTCAAGAACTGAAAGTGGACCTGGGGGTGATAATTCTAAGAGTCAGCTCCAGACATTGCTCACAAGGGCAGGATATGCTCCGCCGACCTACAAAACTAAGCAACTAAAGAACTGTAAATTCCAATCTAGTGTGGAGTTCAATGGAATGCAGATTATGGGGCAGCCTTGTAATAACAAGAAGAGTGCAGAAAAAGATGCAGCAGCTGAGGCCATTCAGTGGCTGGTGAGTGGTACACAAATGGGCCATGAGCACATCAATCACATGTCAATGATGTTGAAGAAAAGCAGAAAAGGACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1214

Amino Acids

135.81

Weight (kDa)

8.8

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 331 - 482 2.8e-10 DEAD/DEAH box helicase
Helicase_C PF00271 590 - 718 3.3e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 782 - 810 6.1e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 811 - 870 1.6e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 937 - 1018 1.1e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1027 - 1085 8.2e-23 C-terminal domain in RNA helicases
dsrm PF00035 1127 - 1188 8.8e-11 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 411, 972
Acc36I ACCTGC 1 cut(s) 3130
Acc65I GGTACC 1 cut(s) 619
AccB1I GGYRCC 5 cut(s) 167, 371, 619, 1621, 1799
AccB7I CCANNNNNTGG 2 cut(s) 2441, 3558
AccBSI CCGCTC 2 cut(s) 130, 201
AccII CGCG 4 cut(s) 360, 405, 1350, 2151
AclWI GGATC 6 cut(s) 1395, 1874, 2477, 2490, 2516, 3264
AcoI YGGCCR 5 cut(s) 1403, 1588, 1920, 3201, 3283
AcsI RAATTY 7 cut(s) 1059, 1837, 2677, 2844, 2946, 3107, 3458
AcuI CTGAAG 3 cut(s) 420, 1865, 1977
AcyI GRCGYC 3 cut(s) 168, 372, 381
AfaI GTAC 6 cut(s) 621, 805, 1126, 1264, 1546, 3574
AflIII ACRYGT 2 cut(s) 414, 3603
AjiI CACGTC 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AloI GAACNNNNNNTCC 2 cut(s) 1200, 1232
Alw21I GWGCWC 2 cut(s) 3036, 3594
AlwI GGATC 6 cut(s) 1395, 1874, 2477, 2490, 2516, 3264
AlwNI CAGNNNCTG 6 cut(s) 322, 443, 2564, 2908, 3545, 3562
Ama87I CYCGRG 4 cut(s) 303, 1707, 2583, 2934
ApoI RAATTY 7 cut(s) 1059, 1837, 2677, 2844, 2946, 3107, 3458
AseI ATTAAT 1 cut(s) 2016
Asp700I GAANNNNTTC 7 cut(s) 756, 867, 1365, 1533, 2287, 2726, 3055
Asp718I GGTACC 1 cut(s) 619
AspA2I CCTAGG 1 cut(s) 1133
AspLEI GCGC 4 cut(s) 170, 360, 374, 2151
AspS9I GGNCC 7 cut(s) 142, 306, 2370, 3292, 3359, 3583, 3635
AsuII TTCGAA 1 cut(s) 1513
AsuNHI GCTAGC 1 cut(s) 1900
AvaI CYCGRG 4 cut(s) 303, 1707, 2583, 2934
AvaII GGWCC 4 cut(s) 142, 3292, 3359, 3635
AvrII CCTAGG 1 cut(s) 1133
BalI TGGCCA 3 cut(s) 1590, 1922, 3203
BamHI GGATCC 1 cut(s) 2482
BanI GGYRCC 5 cut(s) 167, 371, 619, 1621, 1799
BanII GRGCYC 3 cut(s) 819, 1477, 2311
BauI CACGAG 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 2 cut(s) 3036, 3594
BbvCI CCTCAGC 3 cut(s) 278, 695, 3546
BccI CCATC 8 cut(s) 594, 853, 1292, 1955, 2891, 3068, 3193, 3212
BceAI ACGGC 5 cut(s) 100, 155, 181, 385, 1265
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciVI GTATCC 3 cut(s) 2740, 2847, 3092
BclI TGATCA 1 cut(s) 2596
BfmI CTRYAG 5 cut(s) 634, 2243, 2312, 3126, 3231
BfoI RGCGCY 2 cut(s) 171, 375
BfuAI ACCTGC 1 cut(s) 3130
BfuI GTATCC 3 cut(s) 2740, 2847, 3092
BglI GCCNNNNNGGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 2551
BlnI CCTAGG 1 cut(s) 1133
BlpI GCTNAGC 2 cut(s) 1446, 3327
Bme18I GGWCC 4 cut(s) 142, 3292, 3359, 3635
BmeT110I CYCGRG 4 cut(s) 303, 1707, 2583, 2934
BmgBI CACGTC 1 cut(s) 536
BmgT120I GGNCC 7 cut(s) 142, 306, 2370, 3292, 3359, 3583, 3635
BmrI ACTGGG 1 cut(s) 1830
BmsI GCATC 9 cut(s) 973, 1432, 1654, 2059, 2188, 2439, 2752, 3320, 3526
BmtI GCTAGC 1 cut(s) 1904
BmuI ACTGGG 1 cut(s) 1830
BpiI GAAGAC 1 cut(s) 263
BplI GAGNNNNNCTC 6 cut(s) 667, 699, 1439, 1471, 2224, 2256
BpmI CTGGAG 1 cut(s) 3374
Bpu10I CCTNAGC 4 cut(s) 278, 695, 2741, 3546
Bpu1102I GCTNAGC 2 cut(s) 1446, 3327
Bpu14I TTCGAA 1 cut(s) 1513
BpuEI CTTGAG 3 cut(s) 1367, 1655, 2251
Bsa29I ATCGAT 1 cut(s) 2760
BsaAI YACGTR 1 cut(s) 488
BsaBI GATNNNNATC 3 cut(s) 2751, 2802, 3203
BsaHI GRCGYC 3 cut(s) 168, 372, 381
BsaI GGTCTC 2 cut(s) 91, 2580
BsaWI WCCGGW 1 cut(s) 427
Bse118I RCCGGY 4 cut(s) 144, 374, 654, 1401
Bse1I ACTGG 5 cut(s) 836, 1273, 1825, 1923, 2426
Bse3DI GCAATG 1 cut(s) 3395
Bse8I GATNNNNATC 3 cut(s) 2751, 2802, 3203
BseCI ATCGAT 1 cut(s) 2760
BseJI GATNNNNATC 3 cut(s) 2751, 2802, 3203
BseMI GCAATG 1 cut(s) 3395
BseNI ACTGG 5 cut(s) 836, 1273, 1825, 1923, 2426
BseRI GAGGAG 4 cut(s) 24, 141, 712, 892
BseYI CCCAGC 2 cut(s) 964, 2144
BsgI GTGCAG 2 cut(s) 2835, 3546
Bsh1236I CGCG 4 cut(s) 360, 405, 1350, 2151
Bsh1285I CGRYCG 1 cut(s) 403
BshNI GGYRCC 5 cut(s) 167, 371, 619, 1621, 1799
BshVI ATCGAT 1 cut(s) 2760
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 3036, 3594
BsiHKCI CYCGRG 4 cut(s) 303, 1707, 2583, 2934
BsiSI CCGG 8 cut(s) 145, 347, 375, 428, 655, 1402, 2222, 2505
BslFI GGGAC 4 cut(s) 164, 588, 1683, 2233
BsmBI CGTCTC 4 cut(s) 126, 388, 1994, 2180
BsmFI GGGAC 4 cut(s) 164, 588, 1683, 2233
BsmI GAATGC 1 cut(s) 3492
Bso31I GGTCTC 2 cut(s) 91, 2580
BsoBI CYCGRG 4 cut(s) 303, 1707, 2583, 2934
Bsp119I TTCGAA 1 cut(s) 1513
Bsp1286I GDGCHC 5 cut(s) 819, 1477, 2311, 3036, 3594
Bsp1407I TGTACA 1 cut(s) 1124
Bsp1720I GCTNAGC 2 cut(s) 1446, 3327
Bsp19I CCATGG 2 cut(s) 1909, 1917
Bsp68I TCGCGA 1 cut(s) 405
BspDI ATCGAT 1 cut(s) 2760
BspFNI CGCG 4 cut(s) 360, 405, 1350, 2151
BspHI TCATGA 3 cut(s) 1342, 3114, 3217
BspMAI CTGCAG 2 cut(s) 2247, 3235
BspMI ACCTGC 1 cut(s) 3130
BspOI GCTAGC 1 cut(s) 1904
BspPI GGATC 6 cut(s) 1395, 1874, 2477, 2490, 2516, 3264
BspQI GCTCTTC 1 cut(s) 2134
BspT104I TTCGAA 1 cut(s) 1513
BspT107I GGYRCC 5 cut(s) 167, 371, 619, 1621, 1799
BspTNI GGTCTC 2 cut(s) 91, 2580
BsrBI CCGCTC 2 cut(s) 130, 201
BsrDI GCAATG 1 cut(s) 3395
BsrFI RCCGGY 4 cut(s) 144, 374, 654, 1401
BsrGI TGTACA 1 cut(s) 1124
BsrI ACTGG 5 cut(s) 836, 1273, 1825, 1923, 2426
BssAI RCCGGY 4 cut(s) 144, 374, 654, 1401
BssNI GRCGYC 3 cut(s) 168, 372, 381
BssSI CACGAG 1 cut(s) 924
BssT1I CCWWGG 6 cut(s) 715, 1133, 1909, 1917, 2373, 3194
Bst2BI CACGAG 1 cut(s) 924
Bst4CI ACNGT 8 cut(s) 15, 108, 1697, 2316, 2497, 2538, 2594, 3455
Bst6I CTCTTC 4 cut(s) 1262, 2128, 2134, 3515
BstACI GRCGYC 3 cut(s) 168, 372, 381
BstAPI GCANNNNNTGC 3 cut(s) 602, 2564, 3416
BstAUI TGTACA 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 1513
BstDSI CCRYGG 3 cut(s) 1099, 1909, 1917
BstENI CCTNNNNNAGG 2 cut(s) 281, 1872
BstFNI CGCG 4 cut(s) 360, 405, 1350, 2151
BstH2I RGCGCY 2 cut(s) 171, 375
BstHHI GCGC 4 cut(s) 170, 360, 374, 2151
BstMCI CGRYCG 1 cut(s) 403
BstNSI RCATGY 4 cut(s) 418, 1862, 1908, 3607
BstSFI CTRYAG 5 cut(s) 634, 2243, 2312, 3126, 3231
BstUI CGCG 4 cut(s) 360, 405, 1350, 2151
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 4 cut(s) 1387, 2482, 2521, 2551
BstXI CCANNNNNNTGG 1 cut(s) 2958
BstYI RGATCY 4 cut(s) 1387, 2482, 2521, 2551
Bsu15I ATCGAT 1 cut(s) 2760
BsuI GTATCC 3 cut(s) 2740, 2847, 3092
BsuTUI ATCGAT 1 cut(s) 2760
BtgI CCRYGG 3 cut(s) 1099, 1909, 1917
BtrI CACGTC 1 cut(s) 536
BtsI GCAGTG 1 cut(s) 3240
BtsIMutI CAGTG 9 cut(s) 843, 1266, 2072, 2433, 2599, 2879, 3036, 3240, 3563
BtuMI TCGCGA 1 cut(s) 405
BveI ACCTGC 1 cut(s) 3130
CaiI CAGNNNCTG 6 cut(s) 322, 443, 2564, 2908, 3545, 3562
CciI TCATGA 3 cut(s) 1342, 3114, 3217
CfoI GCGC 4 cut(s) 170, 360, 374, 2151
Cfr10I RCCGGY 4 cut(s) 144, 374, 654, 1401
Cfr13I GGNCC 7 cut(s) 142, 306, 2370, 3292, 3359, 3583, 3635
ClaI ATCGAT 1 cut(s) 2760
CseI GACGC 3 cut(s) 328, 370, 2415
Csp6I GTAC 6 cut(s) 620, 804, 1125, 1263, 1545, 3573
CviQI GTAC 6 cut(s) 620, 804, 1125, 1263, 1545, 3573
DinI GGCGCC 2 cut(s) 169, 373
DraI TTTAAA 3 cut(s) 1687, 2274, 2358
EaeI YGGCCR 5 cut(s) 1403, 1588, 1920, 3201, 3283
Eam1104I CTCTTC 4 cut(s) 1262, 2128, 2134, 3515
EarI CTCTTC 4 cut(s) 1262, 2128, 2134, 3515
EciI GGCGGA 3 cut(s) 73, 220, 3411
Eco130I CCWWGG 6 cut(s) 715, 1133, 1909, 1917, 2373, 3194
Eco147I AGGCCT 2 cut(s) 710, 1795
Eco24I GRGCYC 3 cut(s) 819, 1477, 2311
Eco31I GGTCTC 2 cut(s) 91, 2580
Eco32I GATATC 1 cut(s) 2109
Eco47I GGWCC 4 cut(s) 142, 3292, 3359, 3635
Eco57I CTGAAG 3 cut(s) 420, 1865, 1977
Eco88I CYCGRG 4 cut(s) 303, 1707, 2583, 2934
EcoNI CCTNNNNNAGG 2 cut(s) 281, 1872
EcoO109I RGGNCCY 1 cut(s) 2370
EcoRV GATATC 1 cut(s) 2109
EcoT14I CCWWGG 6 cut(s) 715, 1133, 1909, 1917, 2373, 3194
EcoT38I GRGCYC 3 cut(s) 819, 1477, 2311
EgeI GGCGCC 2 cut(s) 169, 373
EheI GGCGCC 2 cut(s) 169, 373
ErhI CCWWGG 6 cut(s) 715, 1133, 1909, 1917, 2373, 3194
Esp3I CGTCTC 4 cut(s) 126, 388, 1994, 2180
FalI AAGNNNNNCTT 6 cut(s) 740, 772, 1156, 1188, 2438, 2470
FaqI GGGAC 4 cut(s) 164, 588, 1683, 2233
FauI CCCGC 3 cut(s) 135, 277, 888
FauNDI CATATG 2 cut(s) 1121, 2065
FbaI TGATCA 1 cut(s) 2596
FriOI GRGCYC 3 cut(s) 819, 1477, 2311
GlaI GCGC 4 cut(s) 169, 359, 373, 2150
GsaI CCCAGC 2 cut(s) 968, 2148
GsuI CTGGAG 1 cut(s) 3374
HaeII RGCGCY 2 cut(s) 171, 375
HapII CCGG 8 cut(s) 145, 347, 375, 428, 655, 1402, 2222, 2505
HgaI GACGC 3 cut(s) 328, 370, 2415
HhaI GCGC 4 cut(s) 170, 360, 374, 2151
Hin1I GRCGYC 3 cut(s) 168, 372, 381
Hin6I GCGC 4 cut(s) 168, 358, 372, 2149
HinP1I GCGC 4 cut(s) 168, 358, 372, 2149
HincII GTYRAC 2 cut(s) 474, 993
HindII GTYRAC 2 cut(s) 474, 993
HindIII AAGCTT 3 cut(s) 883, 909, 1092
HpaI GTTAAC 1 cut(s) 474
HpaII CCGG 8 cut(s) 145, 347, 375, 428, 655, 1402, 2222, 2505
Hpy166II GTNNAC 8 cut(s) 175, 474, 993, 1127, 1616, 2989, 3359, 3575
Hpy8I GTNNAC 8 cut(s) 175, 474, 993, 1127, 1616, 2989, 3359, 3575
Hpy99I CGWCG 2 cut(s) 121, 424
HpyCH4III ACNGT 8 cut(s) 15, 108, 1697, 2316, 2497, 2538, 2594, 3455
HpyCH4IV ACGT 3 cut(s) 177, 487, 535
HpySE526I ACGT 3 cut(s) 177, 487, 535
Hsp92I GRCGYC 3 cut(s) 168, 372, 381
HspAI GCGC 4 cut(s) 168, 358, 372, 2149
KasI GGCGCC 2 cut(s) 167, 371
KpnI GGTACC 1 cut(s) 623
KroI GCCGGC 2 cut(s) 374, 1401
KroNI GCCGGC 2 cut(s) 376, 1403
Ksp22I TGATCA 1 cut(s) 2596
KspAI GTTAAC 1 cut(s) 474
LguI GCTCTTC 1 cut(s) 2134
LmnI GCTCC 9 cut(s) 206, 296, 342, 1103, 2431, 2790, 3039, 3393, 3424
LweI GCATC 9 cut(s) 973, 1432, 1654, 2059, 2188, 2439, 2752, 3320, 3526
MaeII ACGT 3 cut(s) 177, 487, 535
MaeIII GTNAC 7 cut(s) 108, 483, 488, 1315, 1691, 2316, 2325
MbiI CCGCTC 2 cut(s) 130, 201
MfeI CAATTG 2 cut(s) 240, 2216
MflI RGATCY 4 cut(s) 1387, 2482, 2521, 2551
MhlI GDGCHC 5 cut(s) 819, 1477, 2311, 3036, 3594
MlsI TGGCCA 3 cut(s) 1590, 1922, 3203
MluNI TGGCCA 3 cut(s) 1590, 1922, 3203
Mly113I GGCGCC 2 cut(s) 168, 372
MlyI GAGTC 4 cut(s) 1699, 1899, 1974, 3391
MmeI TCCRAC 2 cut(s) 1186, 1706
Mox20I TGGCCA 3 cut(s) 1590, 1922, 3203
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 2 cut(s) 374, 1401
MroXI GAANNNNTTC 7 cut(s) 756, 867, 1365, 1533, 2287, 2726, 3055
MscI TGGCCA 3 cut(s) 1590, 1922, 3203
MslI CAYNNNNRTG 3 cut(s) 591, 3072, 3608
Msp20I TGGCCA 3 cut(s) 1590, 1922, 3203
MspA1I CMGCKG 3 cut(s) 892, 3017, 3545
MspI CCGG 8 cut(s) 145, 347, 375, 428, 655, 1402, 2222, 2505
MunI CAATTG 2 cut(s) 240, 2216
Mva1269I GAATGC 1 cut(s) 3492
MvnI CGCG 4 cut(s) 360, 405, 1350, 2151
NaeI GCCGGC 2 cut(s) 376, 1403
NarI GGCGCC 2 cut(s) 168, 372
NcoI CCATGG 2 cut(s) 1909, 1917
NdeI CATATG 2 cut(s) 1121, 2065
NgoMIV GCCGGC 2 cut(s) 374, 1401
NheI GCTAGC 1 cut(s) 1900
NmeAIII GCCGAG 1 cut(s) 1913
NmuCI GTSAC 3 cut(s) 108, 488, 2316
NruI TCGCGA 1 cut(s) 405
NspI RCATGY 4 cut(s) 418, 1862, 1908, 3607
NspV TTCGAA 1 cut(s) 1513
PaeI GCATGC 2 cut(s) 1862, 1908
PagI TCATGA 3 cut(s) 1342, 3114, 3217
PceI AGGCCT 2 cut(s) 710, 1795
PciI ACATGT 2 cut(s) 414, 3603
PciSI GCTCTTC 1 cut(s) 2134
PctI GAATGC 1 cut(s) 3492
PdiI GCCGGC 2 cut(s) 376, 1403
PdmI GAANNNNTTC 7 cut(s) 756, 867, 1365, 1533, 2287, 2726, 3055
PflMI CCANNNNNTGG 2 cut(s) 2441, 3558
PfoI TCCNGGA 2 cut(s) 2102, 2839
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 4 cut(s) 1699, 1898, 1973, 3390
PluTI GGCGCC 2 cut(s) 171, 375
PpsI GAGTC 4 cut(s) 1699, 1898, 1973, 3390
Ppu21I YACGTR 1 cut(s) 488
PscI ACATGT 2 cut(s) 414, 3603
PshBI ATTAAT 1 cut(s) 2016
PsiI TTATAA 2 cut(s) 411, 972
PspFI CCCAGC 2 cut(s) 964, 2144
PspPI GGNCC 7 cut(s) 142, 306, 2370, 3292, 3359, 3583, 3635
PstI CTGCAG 2 cut(s) 2247, 3235
PstNI CAGNNNCTG 6 cut(s) 322, 443, 2564, 2908, 3545, 3562
PsuI RGATCY 4 cut(s) 1387, 2482, 2521, 2551
PvuI CGATCG 1 cut(s) 403
PvuII CAGCTG 3 cut(s) 892, 3017, 3545
RruI TCGCGA 1 cut(s) 405
RsaI GTAC 6 cut(s) 621, 805, 1126, 1264, 1546, 3574
RsaNI GTAC 6 cut(s) 620, 804, 1125, 1263, 1545, 3573
RseI CAYNNNNRTG 3 cut(s) 591, 3072, 3608
SapI GCTCTTC 1 cut(s) 2134
Sau96I GGNCC 7 cut(s) 142, 306, 2370, 3292, 3359, 3583, 3635
SchI GAGTC 4 cut(s) 1699, 1899, 1974, 3391
SduI GDGCHC 5 cut(s) 819, 1477, 2311, 3036, 3594
SfaNI GCATC 9 cut(s) 973, 1432, 1654, 2059, 2188, 2439, 2752, 3320, 3526
SfcI CTRYAG 5 cut(s) 634, 2243, 2312, 3126, 3231
SfoI GGCGCC 2 cut(s) 169, 373
SfuI TTCGAA 1 cut(s) 1513
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 4 cut(s) 142, 3292, 3359, 3635
SmiMI CAYNNNNRTG 3 cut(s) 591, 3072, 3608
SmlI CTYRAG 3 cut(s) 1382, 1670, 2230
SmoI CTYRAG 3 cut(s) 1382, 1670, 2230
SphI GCATGC 2 cut(s) 1862, 1908
SseBI AGGCCT 2 cut(s) 710, 1795
SspDI GGCGCC 2 cut(s) 167, 371
SspI AATATT 2 cut(s) 1944, 1993
StuI AGGCCT 2 cut(s) 710, 1795
StyI CCWWGG 6 cut(s) 715, 1133, 1909, 1917, 2373, 3194
TaaI ACNGT 8 cut(s) 15, 108, 1697, 2316, 2497, 2538, 2594, 3455
TaiI ACGT 3 cut(s) 180, 490, 538
TaqI TCGA 7 cut(s) 422, 648, 1296, 1513, 2111, 2760, 2918
TatI WGTACW 1 cut(s) 1124
TauI GCSGC 5 cut(s) 236, 328, 381, 557, 3288
TscAI CASTG 9 cut(s) 843, 1273, 2079, 2433, 2599, 2886, 3036, 3240, 3563
TseFI GTSAC 3 cut(s) 108, 488, 2316
Tsp45I GTSAC 3 cut(s) 108, 488, 2316
TspGWI ACGGA 6 cut(s) 193, 481, 2801, 2983, 3170, 3202
TspRI CASTG 9 cut(s) 843, 1273, 2079, 2433, 2599, 2886, 3036, 3240, 3563
Van91I CCANNNNNTGG 2 cut(s) 2441, 3558
VpaK11BI GGWCC 4 cut(s) 142, 3292, 3359, 3635
VspI ATTAAT 1 cut(s) 2016
XagI CCTNNNNNAGG 2 cut(s) 281, 1872
XapI RAATTY 7 cut(s) 1059, 1837, 2677, 2844, 2946, 3107, 3458
XbaI TCTAGA 2 cut(s) 740, 776
XceI RCATGY 4 cut(s) 418, 1862, 1908, 3607
XcmI CCANNNNNNNNNTGG 1 cut(s) 3559
XmaJI CCTAGG 1 cut(s) 1133
XmnI GAANNNNTTC 7 cut(s) 756, 867, 1365, 1533, 2287, 2726, 3055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.