MD13G1033300.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005622
Physical Location & Seq
Reverse (-)
2329516 .. 2337569
8054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1033300.v1.1.491

Sequence Viewer

Length: 3516 bp
ATGAAGGACCGGCCTCCTTCCTCGTACGGCTCCGTCTACGTCCCTCCCCACCACCGCCCCCGCTCCGCCATCACCACCCCCAATTACACCTCCCCTACTTCAGTCGGCTCCAAGCTCCGCGACAACCAGACTGCTGCTGCTCTTAACCAGAGGAGCAGCACCATTGGTGCTCTGGCGTACTACCAGAATCAGCAGCAGCAGCAGCAGCATTTTCAGAAGCCCAAGCTTCAGCACTCTTCTGCTTACGACGACGGCGTTTCCGAGGAAGGCTCCGATCGCGAAGTTCAGTTGCCGTCGTATCCGACACAGAGTCCTTACTCATCTGATAATGAGTGGGAACGGAAATTTACCACGCTGTTACGTGACAAGGAGAAGCAAGAATTGGTTACGAGGGAGAAAAAAGATAGATGCGACTTTGAGAAAATAGCTGCTTTGGCAAGCAGAATGGGGCTTTATAGCCATCTATATTCGAAAGTTGCTGTTTTTAGTAAGGTCCCACTGCCAAACTACAGATTTGATCTGGATGATAGGCGCCCTCAGAGAGAGGTGACCTTGCCTCATGGGTTGCTGAGGAGAGTTGAGGGATATCTTGGAGAGTTCCTTTCCCAGAAGTCTAGGACAAAGGAAGATCTTTCCTTTTCGATATCAAACAGTAGTGGCAGCATTGCTACTGATGAAGGGCTTTTTGAGCAACCAGAATCATTCGCGTCAAGTAAGGGTGTTATGGAAAAGCTTCTTTGGCGGACAAGTACGCAGATGCGTGATAAGCAACAAGCTTGGCAGGAATCTCCTCAAGGTAGAAAAATGCTTGAACTTCGCAGAAGTCTTCCTGCTTATAAAGAGAAGGATGCATTATTGGCAGCAATTTCACGAGATCAGGTTGTTATCATATCAGGTGAAACTGGCTGTGGCAAGACTACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGTTCGTGGAGCTGTTTGTAGTATTATATGTACACAGCCTAGACGAATATCTGCTATGTCTGTTTCTGAAAGAGTTGCTTCAGAGAGAGGGGAGAAATTGGGTGACTCGGTTGGGTATAAGGTTCGCTTAGAAGGTATGAAGGGGAGGGACACTCGCCTTCTCTTTTGCACCACTGGCATTTTGTTGAGAAGATTGCTAGTTGATAGAAATTTGAAAGGTGTAACTCATGTGATTGTTGATGAAATTCATGAACGTGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGGCGACCAGAACTGAGGCTGATTTTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGTAGAGCTCAGATAATTCATGTTCCAGGTTTTACATATCCAGTTCAAACTCATTTTCTGGAGGATGTTCTGGAAATTACGGGCTGCAAATTAACTCCATACAATCAGATTGATGATTATGGTCAAGATAAGATGTGGAAAATGAGCAAACAGGCACCAAGAAAGAGGAAAAGCCAAATTGCTTTTGTTGTTGAGGATGCACTTAAAGCAGCCAATTTTAAGGGGTATAGTCTACAGACTCAGGAGTCTCTAGCATGTTGGAATCCTGATGGTATTGGTTTTTATCTCATCGAGTATCTCTTGTGCAATATCTGTGAGAGTGAAAAGCCAGGTGCCATTCTAGTTTTTATGACTGGGTGGGATGACATCAATTCTCTGAAGGAAAAGTTACATGCCAATCCTCTATTAGGTGATCCAAGCCGAGTCTTGTTACTAGCATGCCATGGTTCTATGGCGAGTACTGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACAAGCATCACGATTAATGATGTTGTTTTTGTACTTGATTGTGGAAAGGCAAAAGAGACTTCATATGACGCACTGAATAATACTCCTTGTTTACTTCCTTCCTGGATTTCCAAGGTTTCTGCCCAACAAAGAAGAGGAAGAGCAGGACGTGTCCAGCCCGGGGAATGTTATCATCTCTATCCTAGATGTGTGTATGACGCATTTGCAGAGTATCAGTTACCAGAGATTTTGAGGACACCTCTGCAGTCTCTGTGTCTGCAAATAAAAAGTTTAAACCTCGGAAGCATTTCTGAGTTCTTATCTAGGGCTCTGCAGTCACCAGAGTTACTTGCGGTGCAAAACGCCATAGAGTATTTAAAGATCACTGGGGCCTTGGATGAGAAAGAGAATCTGACTGTTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGCTAGGCGCCATTTTCAACTGCCTGGATCCAGTTTTGACTATTGTTTCTGGGCTTAGTGTCAGAGATCCTTTCCTAACACCATTTGACAAGAAAGACCTTGCTGAGGCTGCAAAATCTCAGTTTTCCCGAGATAATAGTGATCACCTTGCACTTGTGCGGGCCTATGAGGGCTGGAAGGTTGCTGAAAGAGACTTTGCTGGATATGACTATTGCTGGAAGAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCCCTTAGGAAGGAGTTCTTCTCTTTGCTTAGGGATACTGATCTTGTTGATGCCAACACCACCACTCATAACGCATGGAGTTATGATGAGCATCTCGTCCGTGCAGTTATTTGCTGTGGCCTGTATCCTGGAATCTGCTCTGTTGTGCACAATGAGAAGTCATTTTCACTGAAAACAATGGAGGATGGGCAGGTGCTGCTATACTCGAACTCTGTCAATGCTAGGGATTCCAAAATTCCATATCCGTGGCTGGTTTTCAATGAGAAGATAAAGGTGAACTCTGTTTTCCTACGGGATTCAACAGCTGTGTCTGACTCAGTGCTCCTCCTATTTGGTGGAAGTTTTTCGAAAGGGGATACCGATGGACACTTGAAAATGCTGGGAGGATATTTGGAATTCTTCATGAAGCCTGCTGTAGCAGAAATGTATCAATGCTTAAAAGGGGAACTTGACCAGTTAATTCAAATCAAACTACTGAACCCCAGGATGGACACACACAGTTACCATGAACTTCTTTCTGCAATGCGGTTCCTGCTTTCGGAGGATCAAGGCGAAGGTAGATTTGTATTTGGCCGCCAGGTCCTTGCACTCTCAAAGTCAAAGCCATCTGTGGTGGCAGCACAGCCAGCCTTGGTTTCAAGAACTGAAAGTGGACCCGGGGGTGACAATTCTAAGAGTCAGCTCCAGACATTGCTCACACGAGCAGGATACAACGCTCCTACCTACAAAACTATGCAACTGAAGAACAGCCAGTTCCGAGCTACAGTGGAGTTTAACGGAATGCAAATTATGGGCCACCCATGTAACAACAAGAAGAGTGCAGAAAAAGATGCGGCAGCGGAGGCCATTCAGTGGCTGGTGAGTGGAACACAAATGGGCCATGAGGACATCAATCACATGTCAATGATGTTAAAGAGAAGCAAAAAGGACCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1172

Amino Acids

131.78

Weight (kDa)

8.03

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 286 - 438 2.6e-10 DEAD/DEAH box helicase
Helicase_C PF00271 545 - 673 2.2e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 737 - 765 5.7e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 766 - 825 4.2e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 892 - 973 3.5e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 982 - 1040 3.3e-21 C-terminal domain in RNA helicases
dsrm PF00035 1084 - 1145 6.7e-10 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 837
AarI CACCTGC 1 cut(s) 2758
AasI GACNNNNNNGTC 1 cut(s) 1576
Acc36I ACCTGC 1 cut(s) 2758
AccB1I GGYRCC 4 cut(s) 531, 1486, 1664, 2327
AccB7I CCANNNNNTGG 2 cut(s) 2306, 3429
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 36, 1564
AccII CGCG 3 cut(s) 120, 279, 707
AclWI GGATC 6 cut(s) 1260, 1739, 2342, 2355, 2381, 3129
AcoI YGGCCR 1 cut(s) 3148
AcsI RAATTY 7 cut(s) 344, 924, 1168, 1203, 2542, 2811, 2972
AcuI CTGAAG 5 cut(s) 84, 212, 1023, 1730, 3338
AcyI GRCGYC 2 cut(s) 532, 2328
AfaI GTAC 6 cut(s) 26, 179, 751, 991, 1792, 1899
AfiI CCNNNNNNNGG 9 cut(s) 1739, 1826, 2026, 2306, 2425, 2587, 3064, 3115, 3429
AflIII ACRYGT 2 cut(s) 2014, 3474
AgsI TTSAA 9 cut(s) 812, 1174, 1379, 2338, 2836, 2877, 2950, 3041, 3216
AhdI GACNNNNNGTC 1 cut(s) 309
AjiI CACGTC 1 cut(s) 2015
AjnI CCWGG 7 cut(s) 1357, 1660, 1967, 2343, 2704, 3059, 3153
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 4 cut(s) 172, 1342, 2727, 2901
Alw26I GTCTC 5 cut(s) 1584, 1859, 1916, 2118, 2505
Alw44I GTGCAC 1 cut(s) 2723
AlwI GGATC 6 cut(s) 1260, 1739, 2342, 2355, 2381, 3129
AlwNI CAGNNNCTG 3 cut(s) 2116, 2773, 3433
Ama87I CYCGRG 3 cut(s) 2024, 2448, 3233
AoxI GGCC 8 cut(s) 11, 2235, 2481, 2695, 3148, 3370, 3420, 3454
ApaLI GTGCAC 1 cut(s) 2723
ApoI RAATTY 7 cut(s) 344, 924, 1168, 1203, 2542, 2811, 2972
AseI ATTAAT 1 cut(s) 1881
Asp700I GAANNNNTTC 6 cut(s) 732, 960, 1230, 2152, 2591, 2920
AspLEI GCGC 2 cut(s) 534, 2330
AspS9I GGNCC 9 cut(s) 7, 493, 2235, 2481, 3157, 3230, 3370, 3454, 3505
AsuC2I CCSGG 4 cut(s) 2025, 2026, 3234, 3235
AsuII TTCGAA 2 cut(s) 470, 2924
AvaI CYCGRG 3 cut(s) 2024, 2448, 3233
AvaII GGWCC 5 cut(s) 7, 493, 3157, 3230, 3505
AxyI CCTNAGG 2 cut(s) 1821, 2582
BaeGI GKGCMC 1 cut(s) 2727
BamHI GGATCC 1 cut(s) 2347
BanI GGYRCC 4 cut(s) 531, 1486, 1664, 2327
BanII GRGCYC 2 cut(s) 1342, 2176
BauI CACGAG 2 cut(s) 870, 3276
BbsI GAAGAC 1 cut(s) 818
Bbv12I GWGCWC 4 cut(s) 172, 1342, 2727, 2901
BbvCI CCTCAGC 2 cut(s) 569, 2424
BccI CCATC 8 cut(s) 77, 468, 1595, 1820, 2756, 2933, 3058, 3190
BceAI ACGGC 3 cut(s) 43, 268, 277
BciT130I CCWGG 7 cut(s) 1359, 1662, 1969, 2345, 2706, 3061, 3155
BciVI GTATCC 5 cut(s) 309, 2605, 2712, 2926, 3278
BclI TGATCA 1 cut(s) 2461
BcnI CCSGG 4 cut(s) 2025, 2026, 3234, 3235
BcoDI GTCTC 5 cut(s) 1584, 1859, 1916, 2118, 2505
BfmI CTRYAG 6 cut(s) 508, 1565, 2108, 2177, 2991, 3339
BfoI RGCGCY 2 cut(s) 535, 2331
BfuAI ACCTGC 1 cut(s) 2758
BfuI GTATCC 5 cut(s) 309, 2605, 2712, 2926, 3278
BglII AGATCT 1 cut(s) 628
BmcAI AGTACT 1 cut(s) 1792
Bme18I GGWCC 5 cut(s) 7, 493, 3157, 3230, 3505
BmeRI GACNNNNNGTC 1 cut(s) 309
BmeT110I CYCGRG 3 cut(s) 2024, 2448, 3233
BmgBI CACGTC 1 cut(s) 2015
BmgT120I GGNCC 9 cut(s) 7, 493, 2235, 2481, 3157, 3230, 3370, 3454, 3505
BmrI ACTGGG 2 cut(s) 1695, 2241
BmsI GCATC 9 cut(s) 398, 747, 838, 1519, 1881, 2304, 2617, 2677, 3397
BmuI ACTGGG 2 cut(s) 1695, 2241
BpiI GAAGAC 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 1412, 3245
Bpu10I CCTNAGC 3 cut(s) 569, 2424, 2606
Bpu14I TTCGAA 2 cut(s) 470, 2924
BpuEI CTTGAG 2 cut(s) 777, 1232
BpuMI CCSGG 4 cut(s) 2025, 2026, 3234, 3235
Bsa29I ATCGAT 1 cut(s) 2561
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 2 cut(s) 2616, 2667
BsaHI GRCGYC 2 cut(s) 532, 2328
Bsc4I CCNNNNNNNGG 9 cut(s) 1739, 1826, 2026, 2306, 2425, 2587, 3064, 3115, 3429
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 8 cut(s) 907, 1138, 1373, 1690, 2236, 2351, 3031, 3328
Bse21I CCTNAGG 2 cut(s) 1821, 2582
Bse3DI GCAATG 3 cut(s) 663, 3105, 3266
Bse8I GATNNNNATC 2 cut(s) 2616, 2667
BseBI CCWGG 7 cut(s) 1359, 1662, 1969, 2345, 2706, 3061, 3155
BseCI ATCGAT 1 cut(s) 2561
BseJI GATNNNNATC 2 cut(s) 2616, 2667
BseLI CCNNNNNNNGG 9 cut(s) 1739, 1826, 2026, 2306, 2425, 2587, 3064, 3115, 3429
BseMI GCAATG 3 cut(s) 663, 3105, 3266
BseNI ACTGG 8 cut(s) 907, 1138, 1373, 1690, 2236, 2351, 3031, 3328
BseRI GAGGAG 4 cut(s) 166, 586, 780, 2891
BseSI GKGCMC 1 cut(s) 2727
BseYI CCCAGC 1 cut(s) 2956
BsgI GTGCAG 2 cut(s) 2700, 3417
Bsh1236I CGCG 3 cut(s) 120, 279, 707
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 8 cut(s) 13, 2237, 2483, 2697, 3150, 3372, 3422, 3456
BshNI GGYRCC 4 cut(s) 531, 1486, 1664, 2327
BshVI ATCGAT 1 cut(s) 2561
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 4 cut(s) 172, 1342, 2727, 2901
BsiHKCI CYCGRG 3 cut(s) 2024, 2448, 3233
BsiSI CCGG 3 cut(s) 10, 2025, 3234
BsiWI CGTACG 1 cut(s) 24
BslFI GGGAC 3 cut(s) 26, 479, 1121
BslI CCNNNNNNNGG 9 cut(s) 1739, 1826, 2026, 2306, 2425, 2587, 3064, 3115, 3429
BsmAI GTCTC 5 cut(s) 1584, 1859, 1916, 2118, 2505
BsmFI GGGAC 3 cut(s) 26, 479, 1121
BsmI GAATGC 1 cut(s) 3363
BsnI GGCC 8 cut(s) 13, 2237, 2483, 2697, 3150, 3372, 3422, 3456
BsoBI CYCGRG 3 cut(s) 2024, 2448, 3233
Bsp119I TTCGAA 2 cut(s) 470, 2924
Bsp1286I GDGCHC 5 cut(s) 172, 1342, 2176, 2727, 2901
Bsp1407I TGTACA 1 cut(s) 989
Bsp19I CCATGG 1 cut(s) 1774
Bsp68I TCGCGA 1 cut(s) 279
BspANI GGCC 8 cut(s) 13, 2237, 2483, 2697, 3150, 3372, 3422, 3456
BspDI ATCGAT 1 cut(s) 2561
BspFNI CGCG 3 cut(s) 120, 279, 707
BspHI TCATGA 2 cut(s) 1207, 2979
BspMAI CTGCAG 2 cut(s) 2112, 2181
BspMI ACCTGC 1 cut(s) 2758
BspPI GGATC 6 cut(s) 1260, 1739, 2342, 2355, 2381, 3129
BspQI GCTCTTC 1 cut(s) 1999
BspT104I TTCGAA 2 cut(s) 470, 2924
BspT107I GGYRCC 4 cut(s) 531, 1486, 1664, 2327
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 3 cut(s) 663, 3105, 3266
BsrFI RCCGGY 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 989
BsrI ACTGG 8 cut(s) 907, 1138, 1373, 1690, 2236, 2351, 3031, 3328
BssAI RCCGGY 1 cut(s) 9
BssNI GRCGYC 2 cut(s) 532, 2328
BssSI CACGAG 2 cut(s) 870, 3276
BssT1I CCWWGG 4 cut(s) 1774, 1977, 2238, 3207
Bst2BI CACGAG 2 cut(s) 870, 3276
Bst2UI CCWGG 7 cut(s) 1359, 1662, 1969, 2345, 2706, 3061, 3155
Bst4CI ACNGT 4 cut(s) 653, 2263, 3077, 3343
Bst6I CTCTTC 4 cut(s) 241, 1993, 1999, 3386
BstACI GRCGYC 2 cut(s) 532, 2328
BstAPI GCANNNNNTGC 1 cut(s) 2773
BstAUI TGTACA 1 cut(s) 989
BstBAI YACGTR 1 cut(s) 362
BstBI TTCGAA 2 cut(s) 470, 2924
BstC8I GCNNGC 6 cut(s) 439, 1771, 1848, 2481, 2988, 3204
BstDSI CCRYGG 2 cut(s) 1774, 2822
BstEII GGTNACC 1 cut(s) 547
BstENI CCTNNNNNAGG 3 cut(s) 1737, 2423, 2585
BstFNI CGCG 3 cut(s) 120, 279, 707
BstH2I RGCGCY 2 cut(s) 535, 2331
BstHHI GCGC 2 cut(s) 534, 2330
BstMAI GTCTC 5 cut(s) 1584, 1859, 1916, 2118, 2505
BstMCI CGRYCG 1 cut(s) 277
BstNI CCWGG 7 cut(s) 1359, 1662, 1969, 2345, 2706, 3061, 3155
BstNSI RCATGY 4 cut(s) 1590, 1727, 1773, 3478
BstPI GGTNACC 1 cut(s) 547
BstSFI CTRYAG 6 cut(s) 508, 1565, 2108, 2177, 2991, 3339
BstSLI GKGCMC 1 cut(s) 2727
BstUI CGCG 3 cut(s) 120, 279, 707
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 4 cut(s) 628, 1252, 2347, 2386
BstXI CCANNNNNNTGG 1 cut(s) 2823
BstYI RGATCY 4 cut(s) 628, 1252, 2347, 2386
Bsu15I ATCGAT 1 cut(s) 2561
Bsu36I CCTNAGG 2 cut(s) 1821, 2582
BsuI GTATCC 5 cut(s) 309, 2605, 2712, 2926, 3278
BsuRI GGCC 8 cut(s) 13, 2237, 2483, 2697, 3150, 3372, 3422, 3456
BsuTUI ATCGAT 1 cut(s) 2561
BtgI CCRYGG 2 cut(s) 1774, 2822
BtrI CACGTC 1 cut(s) 2015
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 8 cut(s) 497, 1131, 1937, 2229, 2744, 2901, 3348, 3434
BtuMI TCGCGA 1 cut(s) 279
BveI ACCTGC 1 cut(s) 2758
Cac8I GCNNGC 6 cut(s) 439, 1771, 1848, 2481, 2988, 3204
CaiI CAGNNNCTG 3 cut(s) 2116, 2773, 3433
CciI TCATGA 2 cut(s) 1207, 2979
CfoI GCGC 2 cut(s) 534, 2330
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 9 cut(s) 7, 493, 2235, 2481, 3157, 3230, 3370, 3454, 3505
Cfr9I CCCGGG 2 cut(s) 2024, 3233
ClaI ATCGAT 1 cut(s) 2561
CseI GACGC 4 cut(s) 696, 1943, 2072, 2280
Csp6I GTAC 6 cut(s) 25, 178, 750, 990, 1791, 1898
CviQI GTAC 6 cut(s) 25, 178, 750, 990, 1791, 1898
DinI GGCGCC 2 cut(s) 533, 2329
DraI TTTAAA 2 cut(s) 2139, 2223
DrdI GACNNNNNNGTC 1 cut(s) 1576
DriI GACNNNNNGTC 1 cut(s) 309
DseDI GACNNNNNNGTC 1 cut(s) 1576
EaeI YGGCCR 1 cut(s) 3148
Eam1104I CTCTTC 4 cut(s) 241, 1993, 1999, 3386
Eam1105I GACNNNNNGTC 1 cut(s) 309
EarI CTCTTC 4 cut(s) 241, 1993, 1999, 3386
EciI GGCGGA 2 cut(s) 55, 757
Ecl136II GAGCTC 1 cut(s) 1340
Eco130I CCWWGG 4 cut(s) 1774, 1977, 2238, 3207
Eco24I GRGCYC 2 cut(s) 1342, 2176
Eco32I GATATC 2 cut(s) 587, 645
Eco47I GGWCC 5 cut(s) 7, 493, 3157, 3230, 3505
Eco53kI GAGCTC 1 cut(s) 1340
Eco57I CTGAAG 5 cut(s) 84, 212, 1023, 1730, 3338
Eco81I CCTNAGG 2 cut(s) 1821, 2582
Eco88I CYCGRG 3 cut(s) 2024, 2448, 3233
Eco91I GGTNACC 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 1340
EcoNI CCTNNNNNAGG 3 cut(s) 1737, 2423, 2585
EcoO109I RGGNCCY 3 cut(s) 493, 2235, 3157
EcoO65I GGTNACC 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 2972
EcoRII CCWGG 7 cut(s) 1357, 1660, 1967, 2343, 2704, 3059, 3153
EcoRV GATATC 2 cut(s) 587, 645
EcoT14I CCWWGG 4 cut(s) 1774, 1977, 2238, 3207
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 2 cut(s) 1342, 2176
EgeI GGCGCC 2 cut(s) 533, 2329
EheI GGCGCC 2 cut(s) 533, 2329
ErhI CCWWGG 4 cut(s) 1774, 1977, 2238, 3207
FaqI GGGAC 3 cut(s) 26, 479, 1121
FauI CCCGC 2 cut(s) 68, 2472
FauNDI CATATG 1 cut(s) 1930
FbaI TGATCA 1 cut(s) 2461
FblI GTMKAC 2 cut(s) 36, 1564
FriOI GRGCYC 2 cut(s) 1342, 2176
GlaI GCGC 2 cut(s) 533, 2329
GsaI CCCAGC 1 cut(s) 2960
GsuI CTGGAG 2 cut(s) 1412, 3245
HaeII RGCGCY 2 cut(s) 535, 2331
HaeIII GGCC 8 cut(s) 13, 2237, 2483, 2697, 3150, 3372, 3422, 3456
HapII CCGG 3 cut(s) 10, 2025, 3234
HgaI GACGC 4 cut(s) 696, 1943, 2072, 2280
HhaI GCGC 2 cut(s) 534, 2330
Hin1I GRCGYC 2 cut(s) 532, 2328
Hin6I GCGC 2 cut(s) 532, 2328
HinP1I GCGC 2 cut(s) 532, 2328
HindIII AAGCTT 3 cut(s) 224, 731, 774
HpaII CCGG 3 cut(s) 10, 2025, 3234
Hpy166II GTNNAC 7 cut(s) 37, 992, 1565, 1958, 2725, 2854, 3230
Hpy8I GTNNAC 7 cut(s) 37, 992, 1565, 1958, 2725, 2854, 3230
Hpy99I CGWCG 3 cut(s) 251, 254, 298
HpyCH4III ACNGT 4 cut(s) 653, 2263, 3077, 3343
HpyCH4IV ACGT 4 cut(s) 39, 361, 1213, 2014
HpySE526I ACGT 4 cut(s) 39, 361, 1213, 2014
Hsp92I GRCGYC 2 cut(s) 532, 2328
HspAI GCGC 2 cut(s) 532, 2328
KasI GGCGCC 2 cut(s) 531, 2327
Ksp22I TGATCA 1 cut(s) 2461
LguI GCTCTTC 1 cut(s) 1999
LweI GCATC 9 cut(s) 398, 747, 838, 1519, 1881, 2304, 2617, 2677, 3397
MaeII ACGT 4 cut(s) 39, 361, 1213, 2014
MbiI CCGCTC 1 cut(s) 63
MflI RGATCY 4 cut(s) 628, 1252, 2347, 2386
MhlI GDGCHC 5 cut(s) 172, 1342, 2176, 2727, 2901
Mly113I GGCGCC 2 cut(s) 532, 2328
MlyI GAGTC 7 cut(s) 319, 1058, 1564, 1586, 1764, 2885, 3262
MmeI TCCRAC 2 cut(s) 326, 1571
Mph1103I ATGCAT 1 cut(s) 853
MroXI GAANNNNTTC 6 cut(s) 732, 960, 1230, 2152, 2591, 2920
MslI CAYNNNNRTG 3 cut(s) 1212, 2821, 3479
MspA1I CMGCKG 2 cut(s) 2882, 3416
MspI CCGG 3 cut(s) 10, 2025, 3234
MssI GTTTAAAC 1 cut(s) 2139
Mva1269I GAATGC 1 cut(s) 3363
MvaI CCWGG 7 cut(s) 1359, 1662, 1969, 2345, 2706, 3061, 3155
MvnI CGCG 3 cut(s) 120, 279, 707
NarI GGCGCC 2 cut(s) 532, 2328
NciI CCSGG 4 cut(s) 2025, 2026, 3234, 3235
NcoI CCATGG 1 cut(s) 1774
NdeI CATATG 1 cut(s) 1930
NmeAIII GCCGAG 2 cut(s) 1243, 1778
NmuCI GTSAC 5 cut(s) 362, 547, 1061, 2181, 3239
NruI TCGCGA 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 853
NspI RCATGY 4 cut(s) 1590, 1727, 1773, 3478
NspV TTCGAA 2 cut(s) 470, 2924
PaeI GCATGC 1 cut(s) 1773
PagI TCATGA 2 cut(s) 1207, 2979
PaqCI CACCTGC 1 cut(s) 2758
PciI ACATGT 1 cut(s) 3474
PciSI GCTCTTC 1 cut(s) 1999
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 258
PctI GAATGC 1 cut(s) 3363
PdmI GAANNNNTTC 6 cut(s) 732, 960, 1230, 2152, 2591, 2920
Pfl23II CGTACG 1 cut(s) 24
PflMI CCANNNNNTGG 2 cut(s) 2306, 3429
PfoI TCCNGGA 2 cut(s) 1967, 2704
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 7 cut(s) 318, 1058, 1564, 1585, 1763, 2885, 3261
PluTI GGCGCC 2 cut(s) 535, 2331
PmeI GTTTAAAC 1 cut(s) 2139
PpsI GAGTC 7 cut(s) 318, 1058, 1564, 1585, 1763, 2885, 3261
Ppu21I YACGTR 1 cut(s) 362
PpuMI RGGWCCY 2 cut(s) 493, 3157
PscI ACATGT 1 cut(s) 3474
PshBI ATTAAT 1 cut(s) 1881
PsiI TTATAA 1 cut(s) 837
Psp124BI GAGCTC 1 cut(s) 1342
Psp5II RGGWCCY 2 cut(s) 493, 3157
Psp6I CCWGG 7 cut(s) 1357, 1660, 1967, 2343, 2704, 3059, 3153
PspEI GGTNACC 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 2956
PspGI CCWGG 7 cut(s) 1357, 1660, 1967, 2343, 2704, 3059, 3153
PspLI CGTACG 1 cut(s) 24
PspPI GGNCC 9 cut(s) 7, 493, 2235, 2481, 3157, 3230, 3370, 3454, 3505
PspPPI RGGWCCY 2 cut(s) 493, 3157
PstI CTGCAG 2 cut(s) 2112, 2181
PstNI CAGNNNCTG 3 cut(s) 2116, 2773, 3433
PsuI RGATCY 4 cut(s) 628, 1252, 2347, 2386
PvuI CGATCG 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 2882
RruI TCGCGA 1 cut(s) 279
RsaI GTAC 6 cut(s) 26, 179, 751, 991, 1792, 1899
RsaNI GTAC 6 cut(s) 25, 178, 750, 990, 1791, 1898
RseI CAYNNNNRTG 3 cut(s) 1212, 2821, 3479
SacI GAGCTC 1 cut(s) 1342
SapI GCTCTTC 1 cut(s) 1999
Sau96I GGNCC 9 cut(s) 7, 493, 2235, 2481, 3157, 3230, 3370, 3454, 3505
ScaI AGTACT 1 cut(s) 1792
SchI GAGTC 7 cut(s) 319, 1058, 1564, 1586, 1764, 2885, 3262
SduI GDGCHC 5 cut(s) 172, 1342, 2176, 2727, 2901
SfaNI GCATC 9 cut(s) 398, 747, 838, 1519, 1881, 2304, 2617, 2677, 3397
SfcI CTRYAG 6 cut(s) 508, 1565, 2108, 2177, 2991, 3339
SfoI GGCGCC 2 cut(s) 533, 2329
SfuI TTCGAA 2 cut(s) 470, 2924
SinI GGWCC 5 cut(s) 7, 493, 3157, 3230, 3505
SmaI CCCGGG 2 cut(s) 2026, 3235
SmiMI CAYNNNNRTG 3 cut(s) 1212, 2821, 3479
SmlI CTYRAG 2 cut(s) 792, 1247
SmoI CTYRAG 2 cut(s) 792, 1247
SphI GCATGC 1 cut(s) 1773
SspDI GGCGCC 2 cut(s) 531, 2327
SspI AATATT 2 cut(s) 1809, 1858
SstI GAGCTC 1 cut(s) 1342
StyI CCWWGG 4 cut(s) 1774, 1977, 2238, 3207
TaaI ACNGT 4 cut(s) 653, 2263, 3077, 3343
TaiI ACGT 4 cut(s) 42, 364, 1216, 2017
TaqI TCGA 6 cut(s) 470, 641, 1623, 2561, 2783, 2924
TatI WGTACW 3 cut(s) 989, 1790, 1897
TauI GCSGC 2 cut(s) 3153, 3413
TscAI CASTG 8 cut(s) 504, 1138, 1944, 2236, 2751, 2901, 3348, 3434
TseFI GTSAC 5 cut(s) 362, 547, 1061, 2181, 3239
Tsp45I GTSAC 5 cut(s) 362, 547, 1061, 2181, 3239
TspGWI ACGGA 5 cut(s) 22, 355, 2666, 2811, 3369
TspMI CCCGGG 2 cut(s) 2024, 3233
TspRI CASTG 8 cut(s) 504, 1138, 1944, 2236, 2751, 2901, 3348, 3434
Van91I CCANNNNNTGG 2 cut(s) 2306, 3429
VneI GTGCAC 1 cut(s) 2723
VpaK11BI GGWCC 5 cut(s) 7, 493, 3157, 3230, 3505
VspI ATTAAT 1 cut(s) 1881
XagI CCTNNNNNAGG 3 cut(s) 1737, 2423, 2585
XapI RAATTY 7 cut(s) 344, 924, 1168, 1203, 2542, 2811, 2972
XceI RCATGY 4 cut(s) 1590, 1727, 1773, 3478
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 3430
XmaI CCCGGG 2 cut(s) 2024, 3233
XmiI GTMKAC 2 cut(s) 36, 1564
XmnI GAANNNNTTC 6 cut(s) 732, 960, 1230, 2152, 2591, 2920
ZrmI AGTACT 1 cut(s) 1792
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.