AT2G01740

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005755
Physical Location & Seq
Reverse (-)
326136 .. 327875
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01740.1

Sequence Viewer

Length: 1680 bp
ATGGTCAGAGAAGCTCTTCAATTTCTTTCTCGCCTGAGAAAATCCTCGAATCTCCCAGACCCATTCACCTGCAACAAGCATATTCATCAGCTAATCAATTCCAATTGCGGCATTCTATCTCTCAAATTCTTAGCTTACTTAGTCTCCAGAGGTTACACTCCTCATCGTTCTTCATTCAATTCGGTCGTATCGTTTGTTTGTAAACTAGGGCAAGTTAAATTCGCTGAAGATATCGTTCACTCGATGCCTAGATTCGGGTGTGAACCAGATGTTATATCTTATAACTCTCTCATTGATGGGCATTGTAGAAATGGAGATATCAGAAGTGCTAGTTTGGTTTTAGAGAGTTTAAGAGCCTCTCATGGGTTTATCTGCAAGCCTGACATAGTTAGTTTTAATTCTCTGTTTAATGGGTTTAGCAAGATGAAGATGTTAGATGAGGTTTTTGTGTATATGGGTGTTATGTTGAAGTGTTGTTCGCCGAATGTTGTTACTTATAGTACTTGGATCGACACGTTCTGCAAATCCGGGGAGTTGCAGTTGGCGTTGAAGAGTTTTCACTCTATGAAGAGAGATGCTTTATCTCCAAATGTGGTTACTTTCACTTGTTTGATTGATGGGTACTGTAAAGCTGGTGACTTGGAGGTTGCGGTTTCGTTATACAAAGAAATGAGACGCGTTCGGATGTCTTTGAATGTTGTTACTTATACTGCTTTGATAGATGGTTTCTGCAAGAAGGGGGAAATGCAGAGAGCTGAGGAAATGTATTCAAGGATGGTCGAGGATAGAGTTGAGCCAAACTCTCTTGTATACACTACGATTATCGATGGTTTTTTTCAGAGAGGAGATTCGGATAATGCCATGAAGTTTCTGGCCAAAATGCTCAATCAAGGGATGAGACTTGATATAACAGCTTACGGCGTAATCATATCAGGCCTTTGTGGTAATGGTAAACTAAAGGAGGCAACTGAGATTGTAGAAGATATGGAGAAAAGTGATTTAGTTCCTGATATGGTGATTTTTACTACAATGATGAATGCATATTTTAAATCCGGACGCATGAAGGCTGCGGTTAACATGTACCACAAATTAATTGAGAGAGGCTTTGAGCCCGATGTTGTAGCTCTTTCGACTATGATCGATGGGATTGCGAAGAATGGACAACTACATGAAGCTATAGTTTATTTCTGTATAGAGAAAGCTAATGATGTTATGTACACTGTGCTTATTGATGCTTTGTGCAAAGAAGGAGACTTTATAGAAGTTGAGAGATTGTTTAGTAAAATCTCAGAGGCTGGACTTGTTCCAGATAAGTTCATGTACACTTCTTGGATAGCTGGTTTGTGTAAGCAAGGGAATTTGGTGGATGCGTTTAAGTTAAAAACCAGAATGGTTCAGGAAGGTCTTTTGCTTGATTTGTTAGCCTATACTACATTGATTTACGGGCTAGCTAGCAAGGGTTTGATGGTTGAGGCAAGACAAGTTTTCGATGAAATGTTGAATAGCGGAATAAGTCCTGATTCGGCTGTTTTTGATCTCTTGATTAGAGCTTATGAAAAGGAGGGAAACATGGCCGCTGCTTCGGATTTGCTTCTTGATATGCAGAGAAGGGGGCTTGTAACAGCAGTAAGTGATGCGGATTGCAGCAAACAGTGTGGCAATGAAGTGAACTGTTCGTGA

Protein Analysis

559

Amino Acids

62.49

Weight (kDa)

6.07

Isoelectric Point (pI)

27.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 57 - 103 5.6e-10 PPR repeat family
PPR_3 PF13812 78 - 139 1.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 86 - 115 6.8e-11 PPR repeat
PPR_2 PF13041 127 - 175 1.7e-08 PPR repeat family
PPR_1 PF12854 157 - 190 2.5e-08 PPR repeat
PPR_2 PF13041 161 - 210 3.6e-16 PPR repeat family
PPR_3 PF13812 161 - 208 2.3e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 186 - 230 9.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 194 - 225 1e-12 PPR repeat
PPR_2 PF13041 196 - 245 5.6e-19 PPR repeat family
PPR PF01535 199 - 226 5.9e-09 PPR repeat
TPR_24 PF23276 210 - 324 3.4e-09 Fungal tetratrico peptide repeats
PPR_long PF17177 217 - 367 4.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 220 - 276 1.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 230 - 260 1.6e-10 PPR repeat
PPR_2 PF13041 232 - 278 7.4e-15 PPR repeat family
PPR PF01535 234 - 263 1.6e-08 PPR repeat
TPR_24 PF23276 263 - 363 6.2e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 264 - 294 1.2e-06 PPR repeat
PPR_2 PF13041 266 - 314 3.6e-10 PPR repeat family
PPR_3 PF13812 291 - 348 2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 298 - 330 3.9e-06 PPR repeat
PPR_2 PF13041 302 - 338 5.1e-06 PPR repeat family
PPR_2 PF13041 336 - 385 5.5e-13 PPR repeat family
PPR_2 PF13041 402 - 450 7.3e-09 PPR repeat family
PPR PF01535 404 - 433 3.5e-06 PPR repeat
PPR_1 PF12854 433 - 465 2.6e-07 PPR repeat
PPR_2 PF13041 436 - 484 1.4e-08 PPR repeat family
PPR_3 PF13812 460 - 518 7.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 468 - 499 6.7e-06 PPR repeat
PPR_2 PF13041 473 - 514 1.4e-07 PPR repeat family
PPR PF01535 475 - 504 2.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 678
AccIII TCCGGA 1 cut(s) 1052
AciI CCGC 6 cut(s) 108, 650, 1070, 1506, 1575, 1637
AclWI GGATC 1 cut(s) 515
AcoI YGGCCR 2 cut(s) 873, 1572
AcsI RAATTY 3 cut(s) 125, 218, 1357
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 5 cut(s) 502, 623, 1082, 1217, 1322
AfiI CCNNNNNNNGG 2 cut(s) 254, 363
AflIII ACRYGT 3 cut(s) 513, 676, 1077
AgsI TTSAA 7 cut(s) 20, 178, 469, 550, 694, 771, 1501
Alw26I GTCTC 4 cut(s) 148, 667, 892, 1245
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 1295
Aor13HI TCCGGA 1 cut(s) 1052
AoxI GGCC 3 cut(s) 873, 934, 1572
ApeKI GCWGC 3 cut(s) 1067, 1577, 1644
ApoI RAATTY 3 cut(s) 125, 218, 1357
AseI ATTAAT 1 cut(s) 1091
Asp700I GAANNNNTTC 1 cut(s) 15
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 3 cut(s) 58, 647, 1027
AsuNHI GCTAGC 2 cut(s) 1447, 1451
BalI TGGCCA 1 cut(s) 875
BanII GRGCYC 1 cut(s) 1113
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 3 cut(s) 1054, 1564, 1656
BccI CCATC 7 cut(s) 290, 611, 716, 769, 821, 1136, 1459
BceAI ACGGC 1 cut(s) 934
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 4 cut(s) 148, 667, 892, 1245
BfaI CTAG 5 cut(s) 206, 249, 330, 1448, 1452
BfmI CTRYAG 1 cut(s) 1176
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 5 cut(s) 109, 1068, 1575, 1578, 1645
BlsI GCNGC 5 cut(s) 110, 1069, 1576, 1579, 1646
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 529
BmrFI CCNGG 1 cut(s) 529
BmsI GCATC 5 cut(s) 234, 565, 1222, 1357, 1624
BmtI GCTAGC 2 cut(s) 1451, 1455
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 529
Bsa29I ATCGAT 2 cut(s) 825, 1140
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 1052
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 363
Bse3DI GCAATG 1 cut(s) 1666
BseAI TCCGGA 1 cut(s) 1052
BseCI ATCGAT 2 cut(s) 825, 1140
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 4 cut(s) 690, 780, 900, 1372
BseLI CCNNNNNNNGG 2 cut(s) 254, 363
BseMI GCAATG 1 cut(s) 1666
BseMII CTCAG 4 cut(s) 26, 747, 960, 1302
BseRI GAGGAG 2 cut(s) 150, 858
BseXI GCAGC 3 cut(s) 1054, 1564, 1656
Bsh1236I CGCG 1 cut(s) 678
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 875, 936, 1574
BshVI ATCGAT 2 cut(s) 825, 1140
BsiEI CGRYCG 1 cut(s) 186
BsiSI CCGG 2 cut(s) 528, 1053
BslI CCNNNNNNNGG 2 cut(s) 254, 363
BsmAI GTCTC 4 cut(s) 148, 667, 892, 1245
BsmBI CGTCTC 1 cut(s) 667
BsmI GAATGC 2 cut(s) 111, 1042
BsnI GGCC 3 cut(s) 875, 936, 1574
Bsp1286I GDGCHC 1 cut(s) 1113
Bsp13I TCCGGA 1 cut(s) 1052
Bsp1407I TGTACA 2 cut(s) 1215, 1320
Bsp143I GATC 3 cut(s) 507, 1137, 1534
BspACI CCGC 6 cut(s) 108, 650, 1070, 1506, 1575, 1637
BspANI GGCC 3 cut(s) 875, 936, 1574
BspCNI CTCAG 4 cut(s) 27, 748, 961, 1301
BspDI ATCGAT 2 cut(s) 825, 1140
BspEI TCCGGA 1 cut(s) 1052
BspFNI CGCG 1 cut(s) 678
BspMI ACCTGC 1 cut(s) 77
BspOI GCTAGC 2 cut(s) 1451, 1455
BspPI GGATC 1 cut(s) 515
BspQI GCTCTTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 1666
BsrGI TGTACA 2 cut(s) 1215, 1320
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 3 cut(s) 507, 1137, 1534
BssNAI GTATAC 1 cut(s) 811
Bst1107I GTATAC 1 cut(s) 811
Bst4CI ACNGT 4 cut(s) 626, 1222, 1653, 1673
Bst6I CTCTTC 3 cut(s) 21, 545, 563
BstAUI TGTACA 2 cut(s) 1215, 1320
BstC8I GCNNGC 3 cut(s) 377, 1449, 1453
BstDEI CTNAG 6 cut(s) 35, 130, 139, 756, 969, 1288
BstF5I GGATG 4 cut(s) 690, 780, 900, 1372
BstFNI CGCG 1 cut(s) 678
BstKTI GATC 3 cut(s) 510, 1140, 1537
BstMAI GTCTC 4 cut(s) 148, 667, 892, 1245
BstMBI GATC 3 cut(s) 507, 1137, 1534
BstMCI CGRYCG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 1 cut(s) 527
BstSFI CTRYAG 1 cut(s) 1176
BstUI CGCG 1 cut(s) 678
BstV1I GCAGC 3 cut(s) 1054, 1564, 1656
BstZ17I GTATAC 1 cut(s) 811
Bsu15I ATCGAT 2 cut(s) 825, 1140
BsuRI GGCC 3 cut(s) 875, 936, 1574
BsuTUI ATCGAT 2 cut(s) 825, 1140
BtsCI GGATG 4 cut(s) 690, 780, 900, 1372
BtsIMutI CAGTG 2 cut(s) 1218, 1658
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 3 cut(s) 377, 1449, 1453
CaiI CAGNNNCTG 1 cut(s) 1295
ClaI ATCGAT 2 cut(s) 825, 1140
CseI GACGC 2 cut(s) 684, 1065
Csp6I GTAC 5 cut(s) 501, 622, 1081, 1216, 1321
CspCI CAANNNNNGTGG 2 cut(s) 1636, 1671
CviAII CATG 7 cut(s) 362, 862, 1060, 1078, 1169, 1318, 1570
CviQI GTAC 5 cut(s) 501, 622, 1081, 1216, 1321
DdeI CTNAG 6 cut(s) 35, 130, 139, 756, 969, 1288
DpnI GATC 3 cut(s) 509, 1139, 1536
DpnII GATC 3 cut(s) 507, 1137, 1534
DraI TTTAAA 1 cut(s) 1048
EaeI YGGCCR 2 cut(s) 873, 1572
Eam1104I CTCTTC 3 cut(s) 21, 545, 563
EarI CTCTTC 3 cut(s) 21, 545, 563
Eco147I AGGCCT 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 1113
Eco32I GATATC 2 cut(s) 232, 319
Eco57I CTGAAG 1 cut(s) 246
EcoRV GATATC 2 cut(s) 232, 319
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 1 cut(s) 1113
Esp3I CGTCTC 1 cut(s) 667
FaeI CATG 7 cut(s) 365, 865, 1063, 1081, 1172, 1321, 1573
FatI CATG 7 cut(s) 361, 861, 1059, 1077, 1168, 1317, 1569
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 5 cut(s) 109, 1068, 1575, 1578, 1645
FokI GGATG 4 cut(s) 697, 787, 907, 1379
FriOI GRGCYC 1 cut(s) 1113
Fsp4HI GCNGC 5 cut(s) 109, 1068, 1575, 1578, 1645
FspBI CTAG 5 cut(s) 206, 249, 330, 1448, 1452
GluI GCNGC 5 cut(s) 109, 1068, 1575, 1578, 1645
GsuI CTGGAG 1 cut(s) 130
HaeIII GGCC 3 cut(s) 875, 936, 1574
HapII CCGG 2 cut(s) 528, 1053
HgaI GACGC 2 cut(s) 684, 1065
Hin1II CATG 7 cut(s) 365, 865, 1063, 1081, 1172, 1321, 1573
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HinfI GANTC 4 cut(s) 49, 252, 848, 1520
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 528, 1053
HphI GGTGA 3 cut(s) 58, 647, 1027
Hpy166II GTNNAC 9 cut(s) 203, 238, 263, 811, 953, 1075, 1218, 1323, 1669
Hpy188I TCNGA 7 cut(s) 8, 323, 684, 840, 853, 1291, 1585
Hpy188III TCNNGA 9 cut(s) 147, 1007, 1053, 1307, 1397, 1517, 1540, 1595, 1677
Hpy8I GTNNAC 9 cut(s) 203, 238, 263, 811, 953, 1075, 1218, 1323, 1669
HpyAV CCTTC 5 cut(s) 730, 1057, 1241, 1394, 1602
HpyCH4III ACNGT 4 cut(s) 626, 1222, 1653, 1673
HpyCH4IV ACGT 1 cut(s) 515
HpyF3I CTNAG 6 cut(s) 35, 130, 139, 756, 969, 1288
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 7 cut(s) 365, 865, 1063, 1081, 1172, 1321, 1573
Kpn2I TCCGGA 1 cut(s) 1052
KspAI GTTAAC 1 cut(s) 1075
Kzo9I GATC 3 cut(s) 507, 1137, 1534
LguI GCTCTTC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 1054, 1564, 1656
LweI GCATC 5 cut(s) 234, 565, 1222, 1357, 1624
MaeI CTAG 5 cut(s) 206, 249, 330, 1448, 1452
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 6 cut(s) 152, 490, 595, 635, 700, 1618
MalI GATC 3 cut(s) 509, 1139, 1536
MboI GATC 3 cut(s) 507, 1137, 1534
MboII GAAGA 8 cut(s) 8, 162, 239, 439, 562, 580, 992, 1165
MfeI CAATTG 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 1113
MlsI TGGCCA 1 cut(s) 875
MluI ACGCGT 1 cut(s) 676
MluNI TGGCCA 1 cut(s) 875
Mox20I TGGCCA 1 cut(s) 875
Mph1103I ATGCAT 1 cut(s) 1042
MroI TCCGGA 1 cut(s) 1052
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 1 cut(s) 875
MseI TTAA 9 cut(s) 216, 350, 396, 408, 1047, 1074, 1091, 1374, 1379
Msp20I TGGCCA 1 cut(s) 875
MspA1I CMGCKG 1 cut(s) 1577
MspI CCGG 2 cut(s) 528, 1053
MspR9I CCNGG 1 cut(s) 529
MunI CAATTG 1 cut(s) 103
Mva1269I GAATGC 2 cut(s) 111, 1042
MvnI CGCG 1 cut(s) 678
NciI CCSGG 1 cut(s) 529
NdeII GATC 3 cut(s) 507, 1137, 1534
NheI GCTAGC 2 cut(s) 1447, 1451
NlaIII CATG 7 cut(s) 365, 865, 1063, 1081, 1172, 1321, 1573
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 1 cut(s) 1081
PaqCI CACCTGC 1 cut(s) 77
PceI AGGCCT 1 cut(s) 936
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 2 cut(s) 111, 1042
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 4 cut(s) 49, 252, 848, 1520
PkrI GCNGC 5 cut(s) 110, 1069, 1576, 1579, 1646
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 1 cut(s) 1091
PsiI TTATAA 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 1295
RsaI GTAC 5 cut(s) 502, 623, 1082, 1217, 1322
RsaNI GTAC 5 cut(s) 501, 622, 1081, 1216, 1321
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 9 cut(s) 216, 350, 396, 408, 1047, 1074, 1091, 1374, 1379
SatI GCNGC 5 cut(s) 109, 1068, 1575, 1578, 1645
Sau3AI GATC 3 cut(s) 507, 1137, 1534
ScaI AGTACT 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 1 cut(s) 1113
SfaNI GCATC 5 cut(s) 234, 565, 1222, 1357, 1624
SfcI CTRYAG 1 cut(s) 1176
SseBI AGGCCT 1 cut(s) 936
SsiI CCGC 6 cut(s) 108, 650, 1070, 1506, 1575, 1637
SspMI CTAG 5 cut(s) 206, 249, 330, 1448, 1452
StuI AGGCCT 1 cut(s) 936
StyD4I CCNGG 1 cut(s) 527
TaaI ACNGT 4 cut(s) 626, 1222, 1653, 1673
TaiI ACGT 1 cut(s) 518
TaqI TCGA 8 cut(s) 47, 242, 510, 780, 825, 1130, 1140, 1488
TaqII GACCGA 1 cut(s) 172
TatI WGTACW 3 cut(s) 500, 1215, 1320
TauI GCSGC 2 cut(s) 111, 1577
TfiI GAWTC 4 cut(s) 49, 252, 848, 1520
Tru1I TTAA 9 cut(s) 216, 350, 396, 408, 1047, 1074, 1091, 1374, 1379
Tru9I TTAA 9 cut(s) 216, 350, 396, 408, 1047, 1074, 1091, 1374, 1379
TscAI CASTG 2 cut(s) 1225, 1658
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 3 cut(s) 1067, 1577, 1644
Tsp45I GTSAC 1 cut(s) 635
TspRI CASTG 2 cut(s) 1225, 1658
VspI ATTAAT 1 cut(s) 1091
XapI RAATTY 3 cut(s) 125, 218, 1357
XceI RCATGY 1 cut(s) 1081
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 5 cut(s) 206, 249, 330, 1448, 1452
ZrmI AGTACT 1 cut(s) 502
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.