RchiOBHm_Chr2g0123271

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005755
Physical Location & Seq
Forward (+)
36399625 .. 36400901
1277 bp
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UTR
Exon/CDS
Intron
PRQ49558

Sequence Viewer

Length: 1092 bp
ATGAATGATCAGACAAACCCTCAAATTCTTCGCTCGCTTGCAAAGAACCTCAAAGCCCCAATGTCTTTTGATCTCAATAGGTCCAACTGCGGTGACGTCTCTCTCAAAATCCTCTCTGATTTGGTCTCCAAAGGGTACAAACCTCACCCCTATTCATTCAATTCAGTTATCTCCTTTTTGTGTAAACTGGGTCATGTCAACTCGGCCCAGAAGCTCACAATTTCAATGCCGTTTTTCGGGTGCCGGCCCGATATTGTAACATACAATTGTTTGATTAATGGCTACTGCAAACTCTGTCATATTGATGAGGAGGATAAGGGTAGGAGGGTGGTGTATGGTTATATGGTGAAGGATAAGGTGTCGCCGGATTTGTTTGCTTTTACTTGCTTGATTGATGGGTATTGTAAGGCTGGTAATTTGGAGGTTGCATTTGAATTGCTAGAGGGAATGAAGAAATCGTTATTTTTACCGAATGTGGTGACTTATAGTGCTTTGATTGATGGGCTGTGCAAGAATGGGAAGTTGGAAAGAGCTAAGCGTTTGTTTTGTGAAATGGTGGAACATGGGGTTCGGCCTAGTTCAGCGGTTTACACTTCAATGATAGATGGGCATTTCAAGAAAGGAAATGTAGATGAGGCCATGAAGTATATGAGTAAAATATGGGATGGAAGGGTTAGTCTTGATGCCGCTGCATATGGAGTGGTGGTCTCAGGCCTCTGTAATAATGGTCAGTTCGATAATGCGATGCGGGTTGTTACAGATGATTTCGCCATGAGTGGTTTTTCTCCAGATAAGATGTTGTTGACGACCATTATGGATGTATGTTTCAAAGCTGGCAATTTGAAAGCAGGTTTGGGTGTCTACAGATTGTTAGCAAAGGGTTTTGAACCAGATGCTTTGGTGCTTTCAGCTTTGATGGACGGCTTATGCAAGCATGGGCATTTCGAGGAGGCAAGAGGCTACTTTTGCAAGGAAAAGGCTAATGAGATTTCATATACTGTGCTCATTGATGGGATGTGTAAGGAAGAAAACTTTAGTGAAGTTGAGACGATCTTCAGTGATATGTTGGGCTATGCAGACAATCTTCAGTGA

Protein Analysis

363

Amino Acids

40.32

Weight (kDa)

6.4

Isoelectric Point (pI)

24.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 51 - 97 8.5e-13 PPR repeat family
PPR_1 PF12854 80 - 97 1.1e-07 PPR repeat
PPR_3 PF13812 115 - 167 5.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 120 - 151 2.6e-11 PPR repeat
PPR_2 PF13041 122 - 171 1.7e-17 PPR repeat family
PPR PF01535 126 - 152 4.7e-07 PPR repeat
PPR_1 PF12854 156 - 186 8.7e-14 PPR repeat
PPR_2 PF13041 157 - 203 1.4e-16 PPR repeat family
PPR PF01535 160 - 190 9.8e-11 PPR repeat
TPR_24 PF23276 161 - 251 2.3e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 176 - 292 2.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 189 - 215 7e-06 PPR repeat
PPR_2 PF13041 192 - 241 7.5e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 839
AccB1I GGYRCC 1 cut(s) 240
AccI GTMKAC 1 cut(s) 861
AciI CCGC 4 cut(s) 90, 584, 687, 748
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 1039, 1070
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 8 cut(s) 160, 225, 434, 597, 616, 829, 844, 887
AjuI GAANNNNNNNTTGG 4 cut(s) 506, 538, 836, 868
AluBI AGCT 4 cut(s) 214, 533, 833, 911
AluI AGCT 4 cut(s) 214, 533, 833, 911
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 4 cut(s) 103, 130, 712, 1040
AoxI GGCC 5 cut(s) 204, 245, 572, 636, 712
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 1 cut(s) 24
AseI ATTAAT 1 cut(s) 276
AspS9I GGNCC 3 cut(s) 81, 205, 246
AsuHPI GGTGA 4 cut(s) 104, 137, 358, 490
AvaII GGWCC 1 cut(s) 81
BanI GGYRCC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 1005
BbvI GCAGC 1 cut(s) 676
BccI CCATC 6 cut(s) 389, 494, 599, 659, 910, 1004
BceAI ACGGC 2 cut(s) 214, 937
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 4 cut(s) 103, 130, 712, 1040
BfaI CTAG 2 cut(s) 440, 576
BfmI CTRYAG 1 cut(s) 862
BfuAI ACCTGC 1 cut(s) 839
BisI GCNGC 2 cut(s) 687, 690
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 2 cut(s) 688, 691
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 81, 205, 246
BmiI GGNNCC 1 cut(s) 242
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 3 cut(s) 673, 735, 883
BmuI ACTGGG 1 cut(s) 197
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 130, 712
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 670, 823, 1020
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 2 cut(s) 323, 962
BseXI GCAGC 1 cut(s) 676
BshFI GGCC 5 cut(s) 206, 247, 574, 638, 714
BshNI GGYRCC 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 1005
BsiSI CCGG 2 cut(s) 244, 365
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 4 cut(s) 103, 130, 712, 1040
BsmBI CGTCTC 2 cut(s) 103, 1040
BsnI GGCC 5 cut(s) 206, 247, 574, 638, 714
Bso31I GGTCTC 2 cut(s) 130, 712
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp143I GATC 3 cut(s) 7, 70, 1050
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 4 cut(s) 90, 584, 687, 748
BspANI GGCC 5 cut(s) 206, 247, 574, 638, 714
BspCNI CTCAG 1 cut(s) 722
BspLI GGNNCC 1 cut(s) 242
BspMI ACCTGC 1 cut(s) 839
BspT107I GGYRCC 1 cut(s) 240
BspTNI GGTCTC 2 cut(s) 130, 712
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 243
BssMI GATC 3 cut(s) 7, 70, 1050
BssNI GRCGYC 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 1000
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 5 cut(s) 35, 39, 245, 835, 932
BstDEI CTNAG 2 cut(s) 534, 709
BstF5I GGATG 3 cut(s) 670, 823, 1020
BstKTI GATC 3 cut(s) 10, 73, 1053
BstMAI GTCTC 4 cut(s) 103, 130, 712, 1040
BstMBI GATC 3 cut(s) 7, 70, 1050
BstMWI GCNNNNNNNGC 1 cut(s) 966
BstSFI CTRYAG 1 cut(s) 862
BstV1I GCAGC 1 cut(s) 676
BsuRI GGCC 5 cut(s) 206, 247, 574, 638, 714
BtgZI GCGATG 1 cut(s) 758
BtsCI GGATG 3 cut(s) 670, 823, 1020
BtsIMutI CAGTG 1 cut(s) 1063
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 5 cut(s) 35, 39, 245, 835, 932
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 3 cut(s) 81, 205, 246
Csp6I GTAC 1 cut(s) 136
CviAII CATG 5 cut(s) 194, 563, 640, 772, 935
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 2 cut(s) 534, 709
DpnI GATC 3 cut(s) 9, 72, 1052
DpnII GATC 3 cut(s) 7, 70, 1050
Eco147I AGGCCT 1 cut(s) 714
Eco31I GGTCTC 2 cut(s) 130, 712
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 1039, 1070
Esp3I CGTCTC 2 cut(s) 103, 1040
FaeI CATG 5 cut(s) 197, 566, 643, 775, 938
FatI CATG 5 cut(s) 193, 562, 639, 771, 934
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 694
FbaI TGATCA 1 cut(s) 7
FblI GTMKAC 1 cut(s) 861
Fnu4HI GCNGC 2 cut(s) 687, 690
FokI GGATG 3 cut(s) 677, 830, 1027
Fsp4HI GCNGC 2 cut(s) 687, 690
FspBI CTAG 2 cut(s) 440, 576
GluI GCNGC 2 cut(s) 687, 690
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 5 cut(s) 206, 247, 574, 638, 714
HapII CCGG 2 cut(s) 244, 365
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 5 cut(s) 197, 566, 643, 775, 938
HincII GTYRAC 2 cut(s) 199, 804
HindII GTYRAC 2 cut(s) 199, 804
HpaII CCGG 2 cut(s) 244, 365
HphI GGTGA 4 cut(s) 104, 137, 358, 490
Hpy166II GTNNAC 5 cut(s) 185, 199, 589, 804, 862
Hpy188I TCNGA 2 cut(s) 12, 118
Hpy188III TCNNGA 3 cut(s) 616, 680, 788
Hpy8I GTNNAC 5 cut(s) 185, 199, 589, 804, 862
HpyAV CCTTC 2 cut(s) 343, 663
HpyCH4III ACNGT 1 cut(s) 1000
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 8 cut(s) 41, 288, 428, 510, 692, 930, 969, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 966
HpyF3I CTNAG 2 cut(s) 534, 709
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 5 cut(s) 197, 566, 643, 775, 938
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 3 cut(s) 7, 70, 1050
Lsp1109I GCAGC 1 cut(s) 676
LweI GCATC 3 cut(s) 673, 735, 883
MaeI CTAG 2 cut(s) 440, 576
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 4 cut(s) 92, 256, 478, 754
MalI GATC 3 cut(s) 9, 72, 1052
MboI GATC 3 cut(s) 7, 70, 1050
MboII GAAGA 5 cut(s) 20, 463, 1037, 1045, 1076
MfeI CAATTG 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 1005
MluCI AATT 7 cut(s) 24, 160, 219, 265, 415, 434, 838
MmeI TCCRAC 2 cut(s) 108, 504
MroNI GCCGGC 1 cut(s) 243
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 303, 596
MspA1I CMGCKG 2 cut(s) 584, 689
MspI CCGG 2 cut(s) 244, 365
MunI CAATTG 1 cut(s) 265
MwoI GCNNNNNNNGC 1 cut(s) 966
NaeI GCCGGC 1 cut(s) 245
NdeI CATATG 1 cut(s) 694
NdeII GATC 3 cut(s) 7, 70, 1050
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 5 cut(s) 197, 566, 643, 775, 938
NlaIV GGNNCC 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 2 cut(s) 92, 478
PceI AGGCCT 1 cut(s) 714
PdiI GCCGGC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 688, 691
PshBI ATTAAT 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 3 cut(s) 81, 205, 246
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 2 cut(s) 303, 596
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 687, 690
Sau3AI GATC 3 cut(s) 7, 70, 1050
Sau96I GGNCC 3 cut(s) 81, 205, 246
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 3 cut(s) 673, 735, 883
SfcI CTRYAG 1 cut(s) 862
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 303, 596
Sse9I AATT 7 cut(s) 24, 160, 219, 265, 415, 434, 838
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 4 cut(s) 90, 584, 687, 748
SspMI CTAG 2 cut(s) 440, 576
StuI AGGCCT 1 cut(s) 714
TaaI ACNGT 1 cut(s) 1000
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 735, 945
TasI AATT 7 cut(s) 24, 160, 219, 265, 415, 434, 838
TauI GCSGC 1 cut(s) 689
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 1063
TseFI GTSAC 2 cut(s) 92, 478
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 2 cut(s) 92, 478
TspDTI ATGAA 5 cut(s) 17, 144, 464, 656, 981
TspRI CASTG 1 cut(s) 1063
VpaK11BI GGWCC 1 cut(s) 81
VspI ATTAAT 1 cut(s) 276
XapI RAATTY 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 861
XspI CTAG 2 cut(s) 440, 576
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.