Rh4CG424900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005755
Physical Location & Seq
Reverse (-)
66786530 .. 66787318
789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG424900.1

Sequence Viewer

Length: 789 bp
ATGAAGTATATGAGTAAAATGCAGGATGGAAGGGTTAGTCTTGATGCCGCTGCATATGGAGTGGTGATCTCAGGCCTCTGTAATAATGGTCAGTTTGATAATGCAATGCGGGTTGTTACAGATTTTGCCACGAGTGGTTTTTCTCCAGATAAGATGTTGTTGACGACCATTATGGATGCATGTTTCAAAGCTGGCAATTTGAAAGCAGGTTTGGGTGTCTACAGATTGTTAGCAAAGGGTTTTGAACCAGATGCTGTGGTGCTTTCAGCTTTGATGGACGGCTTATGCAAGCATGGGCGTTTGGAGGAGGCAAGAGGCTACTTTTGCAAGGAAAAGGCTAATGAAATTTCATATACTGTGATCATTGATGGGATGTGTAAGGAAGCAAACTTTAGTGAAGTTGAGATGATCTTCAGTGAGATGTCCGAGATGGGGTTTACTCCAGACAAATATGTATACACTTCTTGGATTGCTGGGCTATGCAGACAGGGTAATCTGGTCGAGGCTTTTAGACTCAAGAATAAAATGGTTAAAGAGGGCATCAGACCTGATCTGTTTACTTATAGCTCACTTATTTTTGGTTTAGCAAGTAAGGGACTCATGGTTGAAGCACAACAGGTTTTCGATGATATGTTGAAAAGAGGAATTACTCCTGACCGTCACGTCTTTGGTATTCTGATGAGAGGGTATTGTGATGAGGGTAATGATGTTGCCATTTTAGGTTTGCACGATGAAATGAGAAAGAGAGGGCTTGTAGTCACAGGAGGTAAAGGAGATGGAGAGCATTGA

Protein Analysis

262

Amino Acids

28.92

Weight (kDa)

5.88

Isoelectric Point (pI)

21.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 79 - 104 7.3e-07 PPR repeat
PPR_2 PF13041 114 - 162 5.6e-12 PPR repeat family
PPR_long PF17177 131 - 236 4.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 137 - 192 1.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 144 - 177 2.6e-08 PPR repeat
PPR PF01535 152 - 181 3.2e-06 PPR repeat
PPR_2 PF13041 153 - 196 4.2e-13 PPR repeat family
PPR_3 PF13812 171 - 230 1.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 180 - 211 3.2e-09 PPR repeat
PPR PF01535 186 - 216 5.9e-07 PPR repeat
PPR_2 PF13041 191 - 232 1.4e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 197
AccI GTMKAC 2 cut(s) 219, 456
AciI CCGC 2 cut(s) 48, 109
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 432
AgsI TTSAA 5 cut(s) 187, 202, 245, 608, 637
AjiI CACGTC 1 cut(s) 664
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 3 cut(s) 191, 269, 567
AluI AGCT 3 cut(s) 191, 269, 567
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 76
BauI CACGAG 1 cut(s) 130
BbvI GCAGC 1 cut(s) 37
BccI CCATC 5 cut(s) 20, 268, 362, 424, 770
BceAI ACGGC 1 cut(s) 295
BclI TGATCA 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 220
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 2 cut(s) 48, 51
BlsI GCNGC 2 cut(s) 49, 52
BmgBI CACGTC 1 cut(s) 664
BmsI GCATC 4 cut(s) 34, 166, 241, 549
BpmI CTGGAG 2 cut(s) 129, 426
BpuEI CTTGAG 1 cut(s) 500
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 111
BseGI GGATG 3 cut(s) 31, 181, 378
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 37
BseYI CCCAGC 1 cut(s) 473
BshFI GGCC 1 cut(s) 75
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 609
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 4 cut(s) 66, 360, 408, 550
BspACI CCGC 2 cut(s) 48, 109
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 83
BspMI ACCTGC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 111
BssMI GATC 4 cut(s) 66, 360, 408, 550
BssNAI GTATAC 1 cut(s) 457
BssSI CACGAG 1 cut(s) 130
Bst1107I GTATAC 1 cut(s) 457
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 2 cut(s) 358, 659
BstC8I GCNNGC 2 cut(s) 193, 290
BstDEI CTNAG 1 cut(s) 70
BstF5I GGATG 3 cut(s) 31, 181, 378
BstKTI GATC 4 cut(s) 69, 363, 411, 553
BstMBI GATC 4 cut(s) 66, 360, 408, 550
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 37
BstZ17I GTATAC 1 cut(s) 457
BsuRI GGCC 1 cut(s) 75
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 3 cut(s) 31, 181, 378
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 2 cut(s) 193, 290
CaiI CAGNNNCTG 1 cut(s) 254
CviAII CATG 3 cut(s) 180, 293, 601
DdeI CTNAG 1 cut(s) 70
DpnI GATC 4 cut(s) 68, 362, 410, 552
DpnII GATC 4 cut(s) 66, 360, 408, 550
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
Eco147I AGGCCT 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 397
EcoT22I ATGCAT 1 cut(s) 181
FaeI CATG 3 cut(s) 183, 296, 604
FaqI GGGAC 1 cut(s) 609
FatI CATG 3 cut(s) 179, 292, 600
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 55
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 2 cut(s) 219, 456
Fnu4HI GCNGC 2 cut(s) 48, 51
FokI GGATG 3 cut(s) 38, 188, 385
Fsp4HI GCNGC 2 cut(s) 48, 51
GluI GCNGC 2 cut(s) 48, 51
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 2 cut(s) 129, 426
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 3 cut(s) 183, 296, 604
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 2 cut(s) 513, 597
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 5 cut(s) 162, 220, 438, 457, 558
Hpy188I TCNGA 3 cut(s) 427, 545, 678
Hpy188III TCNNGA 5 cut(s) 41, 146, 443, 517, 653
Hpy8I GTNNAC 5 cut(s) 162, 220, 438, 457, 558
HpyAV CCTTC 1 cut(s) 24
HpyCH4III ACNGT 2 cut(s) 358, 659
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 8 cut(s) 22, 53, 104, 179, 288, 327, 483, 727
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 1 cut(s) 70
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 3 cut(s) 183, 296, 604
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 4 cut(s) 66, 360, 408, 550
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 4 cut(s) 34, 166, 241, 549
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 3 cut(s) 115, 659, 757
MalI GATC 4 cut(s) 68, 362, 410, 552
MboI GATC 4 cut(s) 66, 360, 408, 550
MboII GAAGA 1 cut(s) 403
MluCI AATT 3 cut(s) 196, 345, 645
MlyI GAGTC 2 cut(s) 507, 591
Mph1103I ATGCAT 1 cut(s) 181
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeI CATATG 1 cut(s) 55
NdeII GATC 4 cut(s) 66, 360, 408, 550
NlaIII CATG 3 cut(s) 183, 296, 604
NmuCI GTSAC 2 cut(s) 659, 757
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 1 cut(s) 183
PceI AGGCCT 1 cut(s) 75
PkrI GCNGC 2 cut(s) 49, 52
PleI GAGTC 2 cut(s) 507, 591
PpsI GAGTC 2 cut(s) 507, 591
PspFI CCCAGC 1 cut(s) 473
PstNI CAGNNNCTG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 2 cut(s) 48, 51
Sau3AI GATC 4 cut(s) 66, 360, 408, 550
SchI GAGTC 2 cut(s) 507, 591
SetI ASST 9 cut(s) 193, 211, 271, 550, 569, 621, 666, 724, 769
SfaNI GCATC 4 cut(s) 34, 166, 241, 549
SfcI CTRYAG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 3 cut(s) 196, 345, 645
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 2 cut(s) 48, 109
StuI AGGCCT 1 cut(s) 75
TaaI ACNGT 2 cut(s) 358, 659
TaiI ACGT 1 cut(s) 666
TaqI TCGA 2 cut(s) 501, 624
TasI AATT 3 cut(s) 196, 345, 645
TauI GCSGC 1 cut(s) 50
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 659, 757
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 2 cut(s) 659, 757
TspDTI ATGAA 4 cut(s) 17, 339, 357, 747
TspRI CASTG 1 cut(s) 421
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 183
XmiI GTMKAC 2 cut(s) 219, 456
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.