RLG00000005963

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005755
Physical Location & Seq
Forward (+)
3099668 .. 3101175
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005963

Sequence Viewer

Length: 1407 bp
ATGATCAGACAAACCCTCAAATTCTTCGCTCGCTTGCGAAGGACCTCAAAGCCCCAGTGTCCTTTGACCTACAACAAGCTCCTCCATAACCTCAATAGGTCCAACTGCGGTGACCTCTCTCTCAAAATCCTCTCTGATTTGGTCTCCAAAGGGTACAATCCTCACCCCTCTTCATTCAATTCAGTTATCTCCTTTTTGTGTAAACTGGGTCATGTTAACTCGGCCCAGAAGCTGACAATTTCAATGCCGATCTTCGGGCGCCGGCCCGATATTGTAACATACAATTGTTTGATTAATGGTGGGTTTTGTAAGGTTAAGATGAGGAAAGATGTGTTTGTGTATATGGGTTTGATGTGGAAGTGTTTTGAACTGAATGTGATTAGTTATAGTACATTGATAGATATGTTTTGCAAGATGGGGGATTTGGATTTGGGATATAGGGTTTATGGTTATATGGTGAAGGATAAGGTGTCGCTGAATTTGTTTGCTTTTACTTGTTTGATTGATGGGTATTGTAAGGCTGGTAATTTGGAGGTTGCATTTGAATTGCTTGAGGGAATGAAGAAATCGTTAGTTTTACCGAATGTGGTGACTTATGGTGCTTTGATTGATGGGCTGTGCAAGAATGGGAAGTTGGAAAGAGCTGAGCGTTTGTTTTGTGAAATGGTGGAAGATGGGGTTCGGCCTAGTTCAGCAGTTTACACTTCAATGATAGATGGGCATTTCAAGAAAGGAAATGTAGGTGAGGCCATGAAGTATATGAGTAAAATGCAGGATGGAAGGGTTAGTGTTGATGCCGCTGCATATGGAGTGGTGATCTCAGGCCTCTGTAATAATGGTCAGTTCGATAATGCGATGCGAGTTGTTACAGATTTTGCCATGAGTGGTTTTTCTCCAGATAAGATGTTGTTGACGACCATTATGGATGCATGTTTCAAAGCCGGCAATTTGAAAGCAGGTTTGGATGTCTACAGATTGTTAGCAAAGGGTTTTGAACCAGATGCTTTGGTGCTTTCAGCTTTGATGGATGGCTTATGCAAGCATGGACATTTGGAGGAGGCAAGAGGCTGCTTCTGCAAGGAAAAGGCTAATGAAATTTCATATACTGTGCTTATTGATGGGATGTGTAAGGAAGAAAACTTTAGTGAAGTTGAGACGATCTTCAGTGAGATGTCCGAAATGGGGTTTATTCCAGACAAATATGTATACATTTCTTGGATTGCTGGGCTATGCAGACAGGGTAATCTGGTTGAGGCTTTTAGACTCAAGAATAAAATGGTTAAAGAGGGCATCAGCCCTGATCTGTTTACTTATAGCTCACTTATTTTTGGTTTAGCAAGTAAGGGACTCATGGTTGAAGCACAACAGGTTTTCGATGATATGTTGAAAAAGAGGAATTACTCCTGA

Protein Analysis

469

Amino Acids

52.39

Weight (kDa)

8.58

Isoelectric Point (pI)

26.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 58 - 100 4.2e-09 PPR repeat family
PPR_1 PF12854 121 - 152 5.8e-07 PPR repeat
PPR_long PF17177 124 - 215 7.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 125 - 173 9.1e-12 PPR repeat family
PPR_3 PF13812 152 - 204 9.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 157 - 188 8e-10 PPR repeat
PPR_2 PF13041 160 - 208 4.7e-16 PPR repeat family
PPR PF01535 163 - 189 6.6e-07 PPR repeat
PPR_1 PF12854 193 - 223 1.4e-13 PPR repeat
PPR_2 PF13041 194 - 240 6.3e-16 PPR repeat family
PPR PF01535 197 - 227 2e-10 PPR repeat
TPR_24 PF23276 198 - 288 5.3e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 213 - 328 4.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 229 - 278 1.3e-10 PPR repeat family
PPR_2 PF13041 364 - 412 5.5e-10 PPR repeat family
PPR PF01535 366 - 396 3.2e-06 PPR repeat
PPR_3 PF13812 387 - 442 4.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 405 - 446 4.2e-12 PPR repeat family
PPR_1 PF12854 430 - 461 3.4e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 947
AccB1I GGYRCC 1 cut(s) 258
AccI GTMKAC 2 cut(s) 969, 1206
AciI CCGC 2 cut(s) 108, 798
AcsI RAATTY 3 cut(s) 20, 478, 1095
AcuI CTGAAG 1 cut(s) 1147
AcyI GRCGYC 1 cut(s) 259
AfaI GTAC 2 cut(s) 155, 391
AfiI CCNNNNNNNGG 2 cut(s) 254, 1182
AjuI GAANNNNNNNTTGG 4 cut(s) 617, 649, 944, 976
AluBI AGCT 5 cut(s) 79, 232, 644, 1019, 1317
AluI AGCT 5 cut(s) 79, 232, 644, 1019, 1317
Alw26I GTCTC 2 cut(s) 148, 1148
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 5 cut(s) 222, 263, 683, 747, 823
ApeKI GCWGC 2 cut(s) 800, 1068
ApoI RAATTY 3 cut(s) 20, 478, 1095
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 4 cut(s) 42, 99, 223, 264
AsuHPI GGTGA 6 cut(s) 122, 155, 469, 601, 755, 826
AvaII GGWCC 2 cut(s) 42, 99
BanI GGYRCC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 787, 1055
BccI CCATC 9 cut(s) 409, 500, 605, 668, 710, 770, 1018, 1022, 1112
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 148, 1148
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 970
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 947
BisI GCNGC 3 cut(s) 798, 801, 1069
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 3 cut(s) 799, 802, 1070
Bme18I GGWCC 2 cut(s) 42, 99
BmgT120I GGNCC 4 cut(s) 42, 99, 223, 264
BmiI GGNNCC 1 cut(s) 260
BmrI ACTGGG 2 cut(s) 49, 215
BmsI GCATC 5 cut(s) 784, 846, 916, 991, 1299
BmuI ACTGGG 2 cut(s) 49, 215
BpmI CTGGAG 1 cut(s) 879
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 572, 1250
BsaHI GRCGYC 1 cut(s) 259
BsaI GGTCTC 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 1182
Bse118I RCCGGY 2 cut(s) 261, 941
Bse1I ACTGG 2 cut(s) 55, 210
BseGI GGATG 5 cut(s) 781, 931, 970, 1033, 1128
BseLI CCNNNNNNNGG 2 cut(s) 254, 1182
BseMII CTCAG 2 cut(s) 636, 834
BseNI ACTGG 2 cut(s) 55, 210
BseRI GAGGAG 2 cut(s) 71, 1070
BseXI GCAGC 2 cut(s) 787, 1055
BseYI CCCAGC 1 cut(s) 1223
BshFI GGCC 5 cut(s) 224, 265, 685, 749, 825
BshNI GGYRCC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 262, 942
BslFI GGGAC 1 cut(s) 1359
BslI CCNNNNNNNGG 2 cut(s) 254, 1182
BsmAI GTCTC 2 cut(s) 148, 1148
BsmBI CGTCTC 1 cut(s) 1148
BsmFI GGGAC 1 cut(s) 1359
BsnI GGCC 5 cut(s) 224, 265, 685, 749, 825
Bso31I GGTCTC 1 cut(s) 148
Bsp143I GATC 5 cut(s) 3, 249, 816, 1158, 1300
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 2 cut(s) 108, 798
BspANI GGCC 5 cut(s) 224, 265, 685, 749, 825
BspCNI CTCAG 2 cut(s) 637, 833
BspLI GGNNCC 1 cut(s) 260
BspMI ACCTGC 1 cut(s) 947
BspT107I GGYRCC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 148
BsrFI RCCGGY 2 cut(s) 261, 941
BsrI ACTGG 2 cut(s) 55, 210
BssAI RCCGGY 2 cut(s) 261, 941
BssMI GATC 5 cut(s) 3, 249, 816, 1158, 1300
BssNAI GTATAC 1 cut(s) 1207
BssNI GRCGYC 1 cut(s) 259
Bst1107I GTATAC 1 cut(s) 1207
Bst4CI ACNGT 1 cut(s) 1108
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 1 cut(s) 259
BstC8I GCNNGC 5 cut(s) 31, 35, 263, 943, 1040
BstDEI CTNAG 2 cut(s) 645, 820
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 5 cut(s) 781, 931, 970, 1033, 1128
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 5 cut(s) 6, 252, 819, 1161, 1303
BstMAI GTCTC 2 cut(s) 148, 1148
BstMBI GATC 5 cut(s) 3, 249, 816, 1158, 1300
BstMWI GCNNNNNNNGC 1 cut(s) 1074
BstNSI RCATGY 1 cut(s) 933
BstPI GGTNACC 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 970
BstV1I GCAGC 2 cut(s) 787, 1055
BstZ17I GTATAC 1 cut(s) 1207
BsuRI GGCC 5 cut(s) 224, 265, 685, 749, 825
BtgZI GCGATG 1 cut(s) 869
BtsCI GGATG 5 cut(s) 781, 931, 970, 1033, 1128
BtsIMutI CAGTG 2 cut(s) 62, 1171
BveI ACCTGC 1 cut(s) 947
Cac8I GCNNGC 5 cut(s) 31, 35, 263, 943, 1040
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 1 cut(s) 261
Cfr10I RCCGGY 2 cut(s) 261, 941
Cfr13I GGNCC 4 cut(s) 42, 99, 223, 264
Csp6I GTAC 2 cut(s) 154, 390
CviAII CATG 6 cut(s) 212, 751, 880, 930, 1043, 1351
CviQI GTAC 2 cut(s) 154, 390
DdeI CTNAG 2 cut(s) 645, 820
DinI GGCGCC 1 cut(s) 260
DpnI GATC 5 cut(s) 5, 251, 818, 1160, 1302
DpnII GATC 5 cut(s) 3, 249, 816, 1158, 1300
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 825
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 42, 99
Eco57I CTGAAG 1 cut(s) 1147
Eco91I GGTNACC 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 42
EcoO65I GGTNACC 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 931
EgeI GGCGCC 1 cut(s) 260
EheI GGCGCC 1 cut(s) 260
Esp3I CGTCTC 1 cut(s) 1148
FaeI CATG 6 cut(s) 215, 754, 883, 933, 1046, 1354
FaqI GGGAC 1 cut(s) 1359
FatI CATG 6 cut(s) 211, 750, 879, 929, 1042, 1350
FauNDI CATATG 1 cut(s) 805
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 969, 1206
Fnu4HI GCNGC 3 cut(s) 798, 801, 1069
FokI GGATG 5 cut(s) 788, 938, 977, 1040, 1135
Fsp4HI GCNGC 3 cut(s) 798, 801, 1069
FspBI CTAG 1 cut(s) 687
GlaI GCGC 1 cut(s) 260
GluI GCNGC 3 cut(s) 798, 801, 1069
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 5 cut(s) 224, 265, 685, 749, 825
HapII CCGG 2 cut(s) 262, 942
HhaI GCGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 259
Hin1II CATG 6 cut(s) 215, 754, 883, 933, 1046, 1354
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 2 cut(s) 217, 912
HindII GTYRAC 2 cut(s) 217, 912
HinfI GANTC 2 cut(s) 1263, 1347
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 2 cut(s) 262, 942
HphI GGTGA 6 cut(s) 122, 155, 469, 601, 755, 826
Hpy166II GTNNAC 7 cut(s) 203, 217, 700, 912, 970, 1207, 1308
Hpy188I TCNGA 3 cut(s) 8, 136, 1177
Hpy188III TCNNGA 5 cut(s) 727, 896, 1193, 1267, 1404
Hpy8I GTNNAC 7 cut(s) 203, 217, 700, 912, 970, 1207, 1308
HpyAV CCTTC 3 cut(s) 33, 454, 774
HpyCH4III ACNGT 1 cut(s) 1108
HpyCH4V TGCA 9 cut(s) 411, 539, 621, 772, 803, 929, 1038, 1077, 1233
HpyF10VI GCNNNNNNNGC 1 cut(s) 1074
HpyF3I CTNAG 2 cut(s) 645, 820
Hsp92I GRCGYC 1 cut(s) 259
Hsp92II CATG 6 cut(s) 215, 754, 883, 933, 1046, 1354
HspAI GCGC 1 cut(s) 259
KasI GGCGCC 1 cut(s) 258
KroI GCCGGC 2 cut(s) 261, 941
KroNI GCCGGC 2 cut(s) 263, 943
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 5 cut(s) 3, 249, 816, 1158, 1300
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 787, 1055
LweI GCATC 5 cut(s) 784, 846, 916, 991, 1299
MaeI CTAG 1 cut(s) 687
MaeIII GTNAC 4 cut(s) 110, 274, 589, 865
MalI GATC 5 cut(s) 5, 251, 818, 1160, 1302
MboI GATC 5 cut(s) 3, 249, 816, 1158, 1300
MboII GAAGA 7 cut(s) 16, 162, 244, 574, 683, 1145, 1153
MfeI CAATTG 1 cut(s) 283
Mly113I GGCGCC 1 cut(s) 259
MlyI GAGTC 2 cut(s) 1257, 1341
MmeI TCCRAC 2 cut(s) 126, 615
Mph1103I ATGCAT 1 cut(s) 931
MroNI GCCGGC 2 cut(s) 261, 941
MseI TTAA 4 cut(s) 216, 294, 315, 1281
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 2 cut(s) 262, 942
MunI CAATTG 1 cut(s) 283
MwoI GCNNNNNNNGC 1 cut(s) 1074
NaeI GCCGGC 2 cut(s) 263, 943
NarI GGCGCC 1 cut(s) 259
NdeI CATATG 1 cut(s) 805
NdeII GATC 5 cut(s) 3, 249, 816, 1158, 1300
NgoMIV GCCGGC 2 cut(s) 261, 941
NlaIII CATG 6 cut(s) 215, 754, 883, 933, 1046, 1354
NlaIV GGNNCC 1 cut(s) 260
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 110, 589
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 1 cut(s) 933
PceI AGGCCT 1 cut(s) 825
PdiI GCCGGC 2 cut(s) 263, 943
PkrI GCNGC 3 cut(s) 799, 802, 1070
PleI GAGTC 2 cut(s) 1257, 1341
PluTI GGCGCC 1 cut(s) 262
PpsI GAGTC 2 cut(s) 1257, 1341
PpuMI RGGWCCY 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 1223
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 4 cut(s) 42, 99, 223, 264
PspPPI RGGWCCY 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 232
RsaI GTAC 2 cut(s) 155, 391
RsaNI GTAC 2 cut(s) 154, 390
RseI CAYNNNNRTG 1 cut(s) 707
SaqAI TTAA 4 cut(s) 216, 294, 315, 1281
SatI GCNGC 3 cut(s) 798, 801, 1069
Sau3AI GATC 5 cut(s) 3, 249, 816, 1158, 1300
Sau96I GGNCC 4 cut(s) 42, 99, 223, 264
SchI GAGTC 2 cut(s) 1257, 1341
SfaNI GCATC 5 cut(s) 784, 846, 916, 991, 1299
SfcI CTRYAG 1 cut(s) 970
SfoI GGCGCC 1 cut(s) 260
SinI GGWCC 2 cut(s) 42, 99
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 2 cut(s) 551, 1265
SmoI CTYRAG 2 cut(s) 551, 1265
SseBI AGGCCT 1 cut(s) 825
SsiI CCGC 2 cut(s) 108, 798
SspDI GGCGCC 1 cut(s) 258
SspMI CTAG 1 cut(s) 687
StuI AGGCCT 1 cut(s) 825
TaaI ACNGT 1 cut(s) 1108
TaqI TCGA 2 cut(s) 846, 1374
TatI WGTACW 1 cut(s) 389
TauI GCSGC 1 cut(s) 800
Tru1I TTAA 4 cut(s) 216, 294, 315, 1281
Tru9I TTAA 4 cut(s) 216, 294, 315, 1281
TscAI CASTG 2 cut(s) 62, 1171
TseFI GTSAC 2 cut(s) 110, 589
TseI GCWGC 2 cut(s) 800, 1068
Tsp45I GTSAC 2 cut(s) 110, 589
TspDTI ATGAA 5 cut(s) 162, 575, 767, 1089, 1107
TspRI CASTG 2 cut(s) 62, 1171
VpaK11BI GGWCC 2 cut(s) 42, 99
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 3 cut(s) 20, 478, 1095
XceI RCATGY 1 cut(s) 933
XmiI GTMKAC 2 cut(s) 969, 1206
XspI CTAG 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.