RLG00000010013

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005755
Physical Location & Seq
Forward (+)
61748131 .. 61748466
336 bp
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UTR
Exon/CDS
Intron
RLM00000010013

Sequence Viewer

Length: 336 bp
ATGAGTAAAATGCGGGATGGAGGGGTTAGTCTTGATGTGGCTGCATACGGAGTGGTGATCTCAGGCCTATGTAAGAATGGTCAGTTAGATAAAGCAATGCGGGTTGTTACAGATTTGGCCATGTGTGGTTTTTCTTCAGATAAGATGTTGTTGACGACCATTATGAATGCATGTTTCAAAGCTGGGAATTTAAAAGCAGCTTTGGGTTTCTACAGAGAATTGTTAGCCAAGGGTTTTGAACCAGATGCTGTGGTGCTTTCAGCTTTGATGGATGGCTTATGCAAGCATATGGATATTGGAGGAGGCAAGAGGCTACTTTTGCAAGAAAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

112

Amino Acids

11.79

Weight (kDa)

8.73

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 8 - 36 7.1e-08 PPR repeat
PPR_2 PF13041 52 - 95 6.8e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 13, 100
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 120
AgsI TTSAA 2 cut(s) 178, 239
AluBI AGCT 3 cut(s) 182, 200, 263
AluI AGCT 3 cut(s) 182, 200, 263
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 2 cut(s) 64, 117
ApeKI GCWGC 2 cut(s) 41, 197
ApoI RAATTY 1 cut(s) 187
AsuHPI GGTGA 1 cut(s) 67
BalI TGGCCA 1 cut(s) 119
BbvI GCAGC 2 cut(s) 28, 209
BccI CCATC 3 cut(s) 11, 262, 266
BfmI CTRYAG 1 cut(s) 211
BisI GCNGC 2 cut(s) 42, 198
BlsI GCNGC 2 cut(s) 43, 199
BmsI GCATC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 2 cut(s) 22, 277
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 315
BseXI GCAGC 2 cut(s) 28, 209
BseYI CCCAGC 1 cut(s) 182
BshFI GGCC 2 cut(s) 66, 119
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 2 cut(s) 66, 119
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 2 cut(s) 13, 100
BspANI GGCC 2 cut(s) 66, 119
BspCNI CTCAG 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 2 cut(s) 22, 277
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 28, 209
BsuRI GGCC 2 cut(s) 66, 119
BtsCI GGATG 2 cut(s) 22, 277
Cac8I GCNNGC 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 248
CviAII CATG 2 cut(s) 121, 171
DdeI CTNAG 1 cut(s) 61
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
DraI TTTAAA 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 2 cut(s) 124, 174
FaiI YATR 8 cut(s) 46, 70, 122, 164, 172, 280, 288, 290
FatI CATG 2 cut(s) 120, 170
FauI CCCGC 2 cut(s) 6, 93
FauNDI CATATG 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 42, 198
FokI GGATG 2 cut(s) 29, 284
Fsp4HI GCNGC 2 cut(s) 42, 198
GluI GCNGC 2 cut(s) 42, 198
GsaI CCCAGC 1 cut(s) 186
HaeIII GGCC 2 cut(s) 66, 119
Hin1II CATG 2 cut(s) 124, 174
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 153
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 153
HpyCH4V TGCA 4 cut(s) 44, 170, 282, 322
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 1 cut(s) 61
Hsp92II CATG 2 cut(s) 124, 174
Kzo9I GATC 1 cut(s) 57
LpnPI CCDG 3 cut(s) 48, 168, 255
Lsp1109I GCAGC 2 cut(s) 28, 209
LweI GCATC 1 cut(s) 235
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 119
MluCI AATT 2 cut(s) 187, 218
MluNI TGGCCA 1 cut(s) 119
MnlI CCTC 4 cut(s) 14, 293, 296, 303
Mox20I TGGCCA 1 cut(s) 119
Mph1103I ATGCAT 1 cut(s) 172
MscI TGGCCA 1 cut(s) 119
MseI TTAA 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 319
NdeI CATATG 1 cut(s) 288
NdeII GATC 1 cut(s) 57
NlaIII CATG 2 cut(s) 124, 174
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PceI AGGCCT 1 cut(s) 66
PctI GAATGC 1 cut(s) 172
PkrI GCNGC 2 cut(s) 43, 199
PspFI CCCAGC 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 248
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 2 cut(s) 42, 198
Sau3AI GATC 1 cut(s) 57
SetI ASST 3 cut(s) 184, 202, 265
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 211
Sse9I AATT 2 cut(s) 187, 218
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 2 cut(s) 13, 100
StuI AGGCCT 1 cut(s) 66
StyI CCWWGG 1 cut(s) 228
TasI AATT 2 cut(s) 187, 218
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TseI GCWGC 2 cut(s) 41, 197
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 63
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 174
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.