AT2G01860

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013270
Physical Location & Seq
Forward (+)
388055 .. 389918
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01860.1

Sequence Viewer

Length: 1461 bp
ATGATACTACACTGTCCTGTCTCTTTGTCACTTAGCTTTCACTTGAATCTTCGAACCAGTAGAATCGGAAACATAGGAGTAACAAGAGTCAACGCTAGTCAAAGGAATCACTCTAAGAAACTCACTAAGAATCTCCGTAATCCACGCCGTACCAAGCTTCCTCCTGATTTTGGTGTTAATTTGTTTCTTAGGAAACCCAAAATTGAACCACTAGTGATTGATGATGATGATGAGCAAGTTCAAGAATCAGTGAATGATGATGATGATGCTGTTGTATGGGAACCAGAAGAAATCGAGGCTATTTCATCGCTGTTTCAGAAAAGGATACCTCAAAAACCCGATAAACCGAGTCGAGTTAGGCCTTTACCACTTCCTCAACCTCACAAGTTACGACCTTTAGGTCTTCCAACTCCAAAGAAGAACATTATCAGATCACCTGCATTGTCTTCTGTATCGAAGCAAGTGTATAAAGATCCAAGCTTTCTCATTGGTTTAGCTAGAGAGATCAAGAGCTTGCCTTCTTCTGATGCAGATGTCTCTCTTGTTCTCAATAAATGGGTTAGTTTCTTGCGTAAAGGGTCACTCTCTACGACTATTCGAGAATTGGGTCATATGGGTTTGCCTGAGAGAGCTTTACAGACGTATCATTGGGCTGAAAAGCATTCTCATTTAGTCCCTGATAACCGGATTCTTGCATCGACTATCCAGGTTTTGGCGAAGCACCATGAGCTGAAACTGCTTAAATTCGACAATAGTTTGGCTAGCAAGAATGTAATTGAAGCTATGATCAAGGGATGCATTGAAGGTGGATGGTTGAATCTAGCTCGGAAGCTTATACTGATCTCGAAGAGTAACAATCGAATACTTGACTCGAGTGTTTACGTGAAGATGATTCTGGAAATAGCTAAAAACCCTGACAAGTACCATCTTGTTGTTGCTCTTCTTGAGGAACTGAAAAAAAGAGAAGATTTGAAATTGAGCCAGCAGGATTGCACAAGTATTATGAAGATCTGTGTGAAACTGGGAGAATTTGAGCTTGTTGAATCTCTCTTTGACTGGTTTAAAGCATCAAACAGAGAACCAAGCGTTGTAATGTACACTACGATGATACATAGCCGGTATTCGGAACAAAAATACCGGGAGGCAATGAGTGTGGTATGGGAGATGGAAGAATCAAATTGTCTTCTTGATCTTCCAGCTTATAGAGTAGTCATTAAACTATTTGTAGCATTGGATGATTTGGGAAGAGCAATGAGGTATTACTCTAAACTCAAGGAAGCTGGATTCTCGCCAACGTATGATATTTATCGCGATATGATTAGCGTTTATACCGCTTCAGGGCGATTGACAAAGTGTAAAGAGATATGTAAGGAGGTTGAAGATGCTGGATTGAGGTTGGATAAAGATACTTCATTTAGGTTACTGCAGCTCGAGAAGCAAACAATGTCTCTTTTGCACTGA

Protein Analysis

486

Amino Acids

55.65

Weight (kDa)

9.25

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 312 - 459 3.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 361 - 407 6.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013270)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01860
fragaria_vesca FvH4_5g25440
malus_domestica MD00G1136800.v1.1 MD16G1284100.v1.1
prunus_persica Prupe.5G010100_v2.0.a1 Prupe.5G010100_v2.0.a1 Prupe.5G010100_v2.0.a1
pyrus_communis pycom16g26000
rosa_chinensis RchiOBHm_Chr5g0066911
rosa_laevigata RLG00000002634 RLG00000035892
rosa_roxburghii Rroxscaffold_1G00014210
rosa_rugosa Rorug05G0379600.1
rosa_samantha Rh5BG456300 Rh5CG478400 Rh5DG471100
rosa_wichuraiana Rw5G041080 Rw7G024910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
AasI GACNNNNNNGTC 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 445
AccB7I CCANNNNNTGG 1 cut(s) 712
AccII CGCG 1 cut(s) 1311
AciI CCGC 1 cut(s) 1332
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 2 cut(s) 743, 1028
AcuI CTGAAG 1 cut(s) 1320
AfaI GTAC 3 cut(s) 151, 923, 1097
AfiI CCNNNNNNNGG 4 cut(s) 170, 712, 1123, 1338
AgsI TTSAA 9 cut(s) 46, 206, 242, 779, 803, 817, 973, 1043, 1379
AhlI ACTAGT 1 cut(s) 211
AjnI CCWGG 1 cut(s) 705
Alw26I GTCTC 3 cut(s) 25, 541, 1452
AlwI GGATC 1 cut(s) 467
Ama87I CYCGRG 2 cut(s) 871, 1430
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 1 cut(s) 1426
ApoI RAATTY 2 cut(s) 743, 1028
AsuC2I CCSGG 1 cut(s) 1139
AsuHPI GGTGA 1 cut(s) 426
AsuII TTCGAA 1 cut(s) 52
AsuNHI GCTAGC 1 cut(s) 761
AvaI CYCGRG 2 cut(s) 871, 1430
BaeI ACNNNNGTAYC 2 cut(s) 626, 659
BbsI GAAGAC 3 cut(s) 395, 438, 1175
BbvI GCAGC 1 cut(s) 1438
BccI CCATC 3 cut(s) 804, 933, 1159
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 707
BciVI GTATCC 1 cut(s) 318
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 1 cut(s) 1139
BcoDI GTCTC 3 cut(s) 25, 541, 1452
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 5 cut(s) 96, 212, 498, 762, 821
BfmI CTRYAG 1 cut(s) 1424
BfuAI ACCTGC 1 cut(s) 445
BfuI GTATCC 1 cut(s) 318
BglII AGATCT 1 cut(s) 1008
BisI GCNGC 1 cut(s) 1427
BlsI GCNGC 1 cut(s) 1428
Bme1390I CCNGG 2 cut(s) 707, 1139
BmeT110I CYCGRG 2 cut(s) 871, 1430
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 2 cut(s) 707, 1139
BmrI ACTGGG 1 cut(s) 1031
BmsI GCATC 6 cut(s) 256, 517, 704, 785, 1076, 1372
BmtI GCTAGC 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 1031
BpiI GAAGAC 3 cut(s) 395, 438, 1175
Bpu14I TTCGAA 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 965, 1256
BpuMI CCSGG 1 cut(s) 1139
BsaAI YACGTR 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 1305
BsaWI WCCGGW 1 cut(s) 684
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 712, 1123, 1338
Bse118I RCCGGY 1 cut(s) 1116
Bse1I ACTGG 3 cut(s) 57, 1026, 1061
Bse3DI GCAATG 2 cut(s) 1152, 1257
Bse8I GATNNNNATC 1 cut(s) 1305
BseBI CCWGG 1 cut(s) 707
BseGI GGATG 3 cut(s) 800, 815, 1240
BseJI GATNNNNATC 1 cut(s) 1305
BseLI CCNNNNNNNGG 4 cut(s) 170, 712, 1123, 1338
BseMI GCAATG 2 cut(s) 1152, 1257
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 3 cut(s) 57, 1026, 1061
BseXI GCAGC 1 cut(s) 1438
Bsh1236I CGCG 1 cut(s) 1311
BshFI GGCC 1 cut(s) 361
BsiHKCI CYCGRG 2 cut(s) 871, 1430
BsiSI CCGG 3 cut(s) 685, 1117, 1138
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 4 cut(s) 170, 712, 1123, 1338
BsmAI GTCTC 3 cut(s) 25, 541, 1452
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 1 cut(s) 361
BsoBI CYCGRG 2 cut(s) 871, 1430
Bsp119I TTCGAA 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 1095
Bsp143I GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
Bsp68I TCGCGA 1 cut(s) 1311
BspACI CCGC 1 cut(s) 1332
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 1311
BspLI GGNNCC 1 cut(s) 282
BspMAI CTGCAG 1 cut(s) 1428
BspMI ACCTGC 1 cut(s) 445
BspOI GCTAGC 1 cut(s) 765
BspPI GGATC 1 cut(s) 467
BspQI GCTCTTC 2 cut(s) 945, 1240
BspT104I TTCGAA 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 1152, 1257
BsrFI RCCGGY 1 cut(s) 1116
BsrGI TGTACA 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 57, 1026, 1061
BssAI RCCGGY 1 cut(s) 1116
BssMI GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 3 cut(s) 842, 945, 1240
BstAUI TGTACA 1 cut(s) 1095
BstBAI YACGTR 1 cut(s) 883
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 515, 763, 983
BstDEI CTNAG 5 cut(s) 32, 114, 126, 188, 624
BstF5I GGATG 3 cut(s) 800, 815, 1240
BstFNI CGCG 1 cut(s) 1311
BstKTI GATC 7 cut(s) 434, 475, 507, 789, 843, 1011, 1192
BstMAI GTCTC 3 cut(s) 25, 541, 1452
BstMBI GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
BstMWI GCNNNNNNNGC 3 cut(s) 727, 736, 1435
BstNI CCWGG 1 cut(s) 707
BstSCI CCNGG 2 cut(s) 705, 1137
BstSFI CTRYAG 1 cut(s) 1424
BstUI CGCG 1 cut(s) 1311
BstV1I GCAGC 1 cut(s) 1438
BstV2I GAAGAC 3 cut(s) 395, 438, 1175
BstX2I RGATCY 2 cut(s) 472, 1008
BstYI RGATCY 2 cut(s) 472, 1008
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 361
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 800, 815, 1240
BtsIMutI CAGTG 3 cut(s) 10, 255, 1456
BtuMI TCGCGA 1 cut(s) 1311
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 515, 763, 983
Cfr10I RCCGGY 1 cut(s) 1116
Csp6I GTAC 3 cut(s) 150, 922, 1096
CspCI CAANNNNNGTGG 2 cut(s) 1134, 1169
CviAII CATG 1 cut(s) 725
CviQI GTAC 3 cut(s) 150, 922, 1096
DdeI CTNAG 5 cut(s) 32, 114, 126, 188, 624
DpnI GATC 7 cut(s) 433, 474, 506, 788, 842, 1010, 1191
DpnII GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
DraI TTTAAA 1 cut(s) 1063
DrdI GACNNNNNNGTC 1 cut(s) 399
DseDI GACNNNNNNGTC 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 842, 945, 1240
EarI CTCTTC 3 cut(s) 842, 945, 1240
Eco147I AGGCCT 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 1320
Eco88I CYCGRG 2 cut(s) 871, 1430
EcoRII CCWGG 1 cut(s) 705
EcoT22I ATGCAT 1 cut(s) 800
FaeI CATG 1 cut(s) 728
FaqI GGGAC 1 cut(s) 659
FatI CATG 1 cut(s) 724
FauNDI CATATG 1 cut(s) 612
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 1 cut(s) 1427
FokI GGATG 3 cut(s) 807, 822, 1247
Fsp4HI GCNGC 1 cut(s) 1427
FspBI CTAG 5 cut(s) 96, 212, 498, 762, 821
GluI GCNGC 1 cut(s) 1427
HaeIII GGCC 1 cut(s) 361
HapII CCGG 3 cut(s) 685, 1117, 1138
Hin1II CATG 1 cut(s) 728
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 3 cut(s) 155, 478, 830
HpaII CCGG 3 cut(s) 685, 1117, 1138
HphI GGTGA 1 cut(s) 426
Hpy166II GTNNAC 3 cut(s) 91, 880, 1098
Hpy188I TCNGA 6 cut(s) 68, 318, 431, 526, 828, 1126
Hpy8I GTNNAC 3 cut(s) 91, 880, 1098
HpyAV CCTTC 2 cut(s) 528, 797
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 3 cut(s) 641, 882, 1295
HpyCH4V TGCA 7 cut(s) 440, 530, 695, 798, 993, 1426, 1456
HpyF10VI GCNNNNNNNGC 3 cut(s) 727, 736, 1435
HpyF3I CTNAG 5 cut(s) 32, 114, 126, 188, 624
HpySE526I ACGT 3 cut(s) 641, 882, 1295
Hsp92II CATG 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
LguI GCTCTTC 2 cut(s) 945, 1240
Lsp1109I GCAGC 1 cut(s) 1438
LweI GCATC 6 cut(s) 256, 517, 704, 785, 1076, 1372
MaeI CTAG 5 cut(s) 96, 212, 498, 762, 821
MaeII ACGT 3 cut(s) 641, 882, 1295
MaeIII GTNAC 6 cut(s) 27, 79, 387, 579, 851, 1419
MalI GATC 7 cut(s) 433, 474, 506, 788, 842, 1010, 1191
MboI GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
MflI RGATCY 2 cut(s) 472, 1008
MluCI AATT 8 cut(s) 178, 201, 602, 743, 774, 974, 1028, 1177
MlyI GAGTC 3 cut(s) 96, 358, 863
MmeI TCCRAC 2 cut(s) 431, 1377
Mph1103I ATGCAT 1 cut(s) 800
MseI TTAA 4 cut(s) 177, 741, 1062, 1215
MslI CAYNNNNRTG 1 cut(s) 1103
MspI CCGG 3 cut(s) 685, 1117, 1138
MspR9I CCNGG 2 cut(s) 707, 1139
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 1 cut(s) 1311
MwoI GCNNNNNNNGC 3 cut(s) 727, 736, 1435
NciI CCSGG 1 cut(s) 1139
NdeI CATATG 1 cut(s) 612
NdeII GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
NheI GCTAGC 1 cut(s) 761
NlaIII CATG 1 cut(s) 728
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 2 cut(s) 27, 579
NruI TCGCGA 1 cut(s) 1311
NsiI ATGCAT 1 cut(s) 800
NspV TTCGAA 1 cut(s) 52
PaeR7I CTCGAG 2 cut(s) 871, 1430
PaqCI CACCTGC 1 cut(s) 445
PceI AGGCCT 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 945, 1240
PctI GAATGC 1 cut(s) 661
PflMI CCANNNNNTGG 1 cut(s) 712
PkrI GCNGC 1 cut(s) 1428
PleI GAGTC 3 cut(s) 95, 357, 863
PpsI GAGTC 3 cut(s) 95, 357, 863
Ppu21I YACGTR 1 cut(s) 883
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 282
PspXI VCTCGAGB 1 cut(s) 871
PstI CTGCAG 1 cut(s) 1428
PsuI RGATCY 2 cut(s) 472, 1008
RruI TCGCGA 1 cut(s) 1311
RsaI GTAC 3 cut(s) 151, 923, 1097
RsaNI GTAC 3 cut(s) 150, 922, 1096
RseI CAYNNNNRTG 1 cut(s) 1103
SapI GCTCTTC 2 cut(s) 945, 1240
SaqAI TTAA 4 cut(s) 177, 741, 1062, 1215
SatI GCNGC 1 cut(s) 1427
Sau3AI GATC 7 cut(s) 431, 472, 504, 786, 840, 1008, 1189
SchI GAGTC 3 cut(s) 96, 358, 863
ScrFI CCNGG 2 cut(s) 707, 1139
SfaNI GCATC 6 cut(s) 256, 517, 704, 785, 1076, 1372
SfcI CTRYAG 1 cut(s) 1424
Sfr274I CTCGAG 2 cut(s) 871, 1430
SfuI TTCGAA 1 cut(s) 52
SlaI CTCGAG 2 cut(s) 871, 1430
SmiMI CAYNNNNRTG 1 cut(s) 1103
SmlI CTYRAG 4 cut(s) 871, 944, 1271, 1430
SmoI CTYRAG 4 cut(s) 871, 944, 1271, 1430
SpeI ACTAGT 1 cut(s) 211
Sse9I AATT 8 cut(s) 178, 201, 602, 743, 774, 974, 1028, 1177
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 1 cut(s) 1332
SspMI CTAG 5 cut(s) 96, 212, 498, 762, 821
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 705, 1137
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 3 cut(s) 644, 885, 1298
TasI AATT 8 cut(s) 178, 201, 602, 743, 774, 974, 1028, 1177
TatI WGTACW 1 cut(s) 1095
Tru1I TTAA 4 cut(s) 177, 741, 1062, 1215
Tru9I TTAA 4 cut(s) 177, 741, 1062, 1215
TscAI CASTG 2 cut(s) 17, 255
TseFI GTSAC 2 cut(s) 27, 579
TseI GCWGC 1 cut(s) 1426
Tsp45I GTSAC 2 cut(s) 27, 579
TspDTI ATGAA 3 cut(s) 294, 1019, 1401
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 17, 255
Van91I CCANNNNNTGG 1 cut(s) 712
XapI RAATTY 2 cut(s) 743, 1028
XhoI CTCGAG 2 cut(s) 871, 1430
XspI CTAG 5 cut(s) 96, 212, 498, 762, 821
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.