AT2G16930

Ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003149
Physical Location & Seq
Forward (+)
7341078 .. 7342317
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16930.4

Sequence Viewer

Length: 465 bp
ATGAATTTCTTAAACTCAGCTGCATCCATTTGCAGAAGAGTTAGTCTGAGGGAACTCATCACTGAGGTTCCTGCTTATACTGGCAGCAGCATTTCCGATGGTTCTTCAAGTGGGTTGAGTTTGGTCTTGAAGCGTTGGGCTACTAAGAAAACCGCTGGTTCTACAAAGAACGGTCGTGACTCTAATCCCAAGTTTCTTGGTGTTAAGAAATTCGGAGGAGAGAGTGTGATACCTGGAAACATCATAGTTCGTCAACGTGGAACTCGGTTTCATCCTGGAGACTATGTCGGGATTGGTAAGGACCATACTCTGTTTGCATTGAAGGAAGGACGAGTCAGGTTCGAGAAAAGCAAGATTACAGGACGCAAATGGATTCATGTTGATCCAATAGGTGGTCATGTTCTTCACCCTATCTACACAAAAGCCGCAGCTGCAAAATCGACTAAGTTGAACACAGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

154

Amino Acids

16.68

Weight (kDa)

10.5

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 47 - 127 1.2e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 392
AciI CCGC 2 cut(s) 153, 426
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 2 cut(s) 4, 209
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 4 cut(s) 108, 130, 322, 451
AjnI CCWGG 2 cut(s) 232, 274
AluBI AGCT 3 cut(s) 20, 431, 458
AluI AGCT 3 cut(s) 20, 431, 458
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 1 cut(s) 377
ApeKI GCWGC 5 cut(s) 20, 84, 87, 428, 431
ApoI RAATTY 2 cut(s) 4, 209
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 398
AvaII GGWCC 1 cut(s) 301
BbvI GCAGC 5 cut(s) 7, 96, 99, 418, 440
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 2 cut(s) 234, 276
BcoDI GTCTC 1 cut(s) 273
BisI GCNGC 6 cut(s) 21, 85, 88, 426, 429, 432
BlsI GCNGC 6 cut(s) 22, 86, 89, 427, 430, 433
Bme1390I CCNGG 2 cut(s) 234, 276
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 2 cut(s) 234, 276
BmsI GCATC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 297
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 2 cut(s) 234, 276
BseGI GGATG 2 cut(s) 23, 271
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMII CTCAG 3 cut(s) 30, 38, 54
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 5 cut(s) 7, 96, 99, 418, 440
Bsh1285I CGRYCG 1 cut(s) 175
BsiEI CGRYCG 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 2 cut(s) 153, 426
BspCNI CTCAG 3 cut(s) 29, 39, 55
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 377
BsrI ACTGG 1 cut(s) 85
BssMI GATC 1 cut(s) 382
Bst2UI CCWGG 2 cut(s) 234, 276
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 31
BstDEI CTNAG 5 cut(s) 16, 47, 63, 144, 444
BstF5I GGATG 2 cut(s) 23, 271
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 1 cut(s) 382
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 2 cut(s) 234, 276
BstSCI CCNGG 2 cut(s) 232, 274
BstV1I GCAGC 5 cut(s) 7, 96, 99, 418, 440
BtsCI GGATG 2 cut(s) 23, 271
BtsIMutI CAGTG 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 1 cut(s) 372
CviAII CATG 2 cut(s) 377, 398
CviJI RGCY 5 cut(s) 20, 140, 425, 431, 458
CviKI_1 RGCY 5 cut(s) 20, 140, 425, 431, 458
DdeI CTNAG 5 cut(s) 16, 47, 63, 144, 444
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 232, 274
FaeI CATG 2 cut(s) 380, 401
FaiI YATR 7 cut(s) 78, 245, 285, 306, 378, 399, 463
FatI CATG 2 cut(s) 376, 397
Fnu4HI GCNGC 6 cut(s) 21, 85, 88, 426, 429, 432
FokI GGATG 2 cut(s) 10, 258
Fsp4HI GCNGC 6 cut(s) 21, 85, 88, 426, 429, 432
GluI GCNGC 6 cut(s) 21, 85, 88, 426, 429, 432
GsuI CTGGAG 1 cut(s) 297
HgaI GACGC 1 cut(s) 372
Hin1II CATG 2 cut(s) 380, 401
HincII GTYRAC 1 cut(s) 254
HindII GTYRAC 1 cut(s) 254
HinfI GANTC 3 cut(s) 179, 333, 373
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 3 cut(s) 48, 97, 215
Hpy188III TCNNGA 4 cut(s) 127, 176, 289, 343
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 2 cut(s) 316, 320
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 4 cut(s) 23, 33, 317, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 5 cut(s) 16, 47, 63, 144, 444
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 2 cut(s) 380, 401
Kzo9I GATC 1 cut(s) 382
LpnPI CCDG 9 cut(s) 66, 84, 141, 219, 246, 261, 288, 322, 345
Lsp1109I GCAGC 5 cut(s) 7, 96, 99, 418, 440
LweI GCATC 1 cut(s) 32
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 3 cut(s) 48, 96, 395
MluCI AATT 2 cut(s) 4, 209
MlyI GAGTC 2 cut(s) 173, 342
MnlI CCTC 3 cut(s) 42, 58, 209
MseI TTAA 2 cut(s) 11, 204
MspA1I CMGCKG 3 cut(s) 20, 155, 431
MspR9I CCNGG 2 cut(s) 234, 276
MvaI CCWGG 2 cut(s) 234, 276
MwoI GCNNNNNNNGC 1 cut(s) 431
NdeII GATC 1 cut(s) 382
NlaIII CATG 2 cut(s) 380, 401
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 373
PflFI GACNNNGTC 1 cut(s) 284
PflMI CCANNNNNTGG 1 cut(s) 392
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 6 cut(s) 22, 86, 89, 427, 430, 433
PleI GAGTC 2 cut(s) 173, 341
PpsI GAGTC 2 cut(s) 173, 341
Psp6I CCWGG 2 cut(s) 232, 274
PspGI CCWGG 2 cut(s) 232, 274
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 2 cut(s) 20, 431
SaqAI TTAA 2 cut(s) 11, 204
SatI GCNGC 6 cut(s) 21, 85, 88, 426, 429, 432
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 173, 342
ScrFI CCNGG 2 cut(s) 234, 276
SetI ASST 8 cut(s) 22, 69, 235, 259, 341, 394, 433, 460
SfaNI GCATC 1 cut(s) 32
SinI GGWCC 1 cut(s) 301
Sse9I AATT 2 cut(s) 4, 209
SsiI CCGC 2 cut(s) 153, 426
StyD4I CCNGG 2 cut(s) 232, 274
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 1 cut(s) 259
TaqI TCGA 2 cut(s) 342, 440
TasI AATT 2 cut(s) 4, 209
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 2 cut(s) 11, 204
Tru9I TTAA 2 cut(s) 11, 204
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 5 cut(s) 20, 84, 87, 428, 431
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 4 cut(s) 17, 260, 365, 450
TspRI CASTG 1 cut(s) 67
Tth111I GACNNNGTC 1 cut(s) 284
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 2 cut(s) 4, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.