FvH4_4g12570

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003149
Physical Location & Seq
Reverse (-)
16243855 .. 16248460
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12570.t1

Sequence Viewer

Length: 447 bp
ATGAATTTTGGAACATCACTATGCCAAAGATTGAATATAAAGCATCTGGTTACCAGTACTCCTGTTTACAGCAGTGCTTCTGATGTCACAGGAGAAGGATTGAGTTTGATGTTCAGGCGCTGGGCTTCTAAAAGAGCAGGTGGATCCACAAAAAATGGGCGAGACTCTCAACCTAAGAATCTTGGAGTTAAGAAATACGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAAGATCACACTCTTTTTGCTCTAAAAGAAGGCACAGTAAAGTTTGAAAAGCACAAGCTCAGTGGACGTAAGTGGGTGCATATAGAGCCCAAAGAAGGTCATGTGCTTCACCCTATATATGCAAATACTACAGCAGCTGAGATGGTGAAGACAGTTGAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

149

Amino Acids

16.39

Weight (kDa)

10.19

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 121 5.8e-37 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 245
AclWI GGATC 2 cut(s) 138, 151
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 34, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 2 cut(s) 340, 419
AluI AGCT 2 cut(s) 340, 419
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 2 cut(s) 138, 151
AlwNI CAGNNNCTG 2 cut(s) 120, 419
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 383, 439
BaeGI GKGCMC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 264, 418
BciT130I CCWGG 2 cut(s) 219, 261
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 445
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 418
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 2 cut(s) 145, 247
BmrFI CCNGG 2 cut(s) 219, 261
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 437
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 355, 411
BseNI ACTGG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 1 cut(s) 428
BseYI CCCAGC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 248, 372
Bsp143I GATC 2 cut(s) 143, 286
BspCNI CTCAG 2 cut(s) 354, 412
BspLI GGNNCC 2 cut(s) 145, 247
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 2 cut(s) 138, 151
BspT107I GGYRCC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 54
BssMI GATC 2 cut(s) 143, 286
Bst2UI CCWGG 2 cut(s) 219, 261
Bst4CI ACNGT 3 cut(s) 200, 319, 436
BstDEI CTNAG 3 cut(s) 174, 341, 420
BstEII GGTNACC 1 cut(s) 49
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 2 cut(s) 243, 367
BstNI CCWGG 2 cut(s) 219, 261
BstPI GGTNACC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 217, 259
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 428
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 349
BveI ACCTGC 1 cut(s) 128
CaiI CAGNNNCTG 2 cut(s) 120, 419
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 1 cut(s) 383
CviJI RGCY 4 cut(s) 125, 340, 370, 419
CviKI_1 RGCY 4 cut(s) 125, 340, 370, 419
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 174, 341, 420
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 217, 259
EcoT38I GRGCYC 1 cut(s) 372
FaeI CATG 1 cut(s) 386
FaiI YATR 9 cut(s) 22, 38, 270, 363, 365, 384, 398, 400, 402
FatI CATG 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 1 cut(s) 445
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 417
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HhaI GCGC 1 cut(s) 120
Hin1II CATG 1 cut(s) 386
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 4 cut(s) 164, 178, 252, 440
HphI GGTGA 2 cut(s) 383, 439
Hpy166II GTNNAC 2 cut(s) 67, 347
Hpy188I TCNGA 1 cut(s) 82
Hpy8I GTNNAC 2 cut(s) 67, 347
HpyAV CCTTC 3 cut(s) 89, 305, 371
HpyCH4III ACNGT 3 cut(s) 200, 319, 436
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 361, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 367
HpyF3I CTNAG 3 cut(s) 174, 341, 420
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 1 cut(s) 386
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 445
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 49, 85
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 442
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 248, 372
MluCI AATT 2 cut(s) 4, 265
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 1 cut(s) 234
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 219, 261
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 2 cut(s) 243, 367
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 1 cut(s) 386
NlaIV GGNNCC 2 cut(s) 145, 247
NmuCI GTSAC 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 178, 252
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 217, 259
PspEI GGTNACC 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 2 cut(s) 145, 247
PstNI CAGNNNCTG 2 cut(s) 120, 419
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 2 cut(s) 143, 286
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 2 cut(s) 248, 372
SetI ASST 7 cut(s) 142, 175, 220, 342, 352, 382, 421
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 2 cut(s) 4, 265
SspMI CTAG 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 217, 259
TaaI ACNGT 3 cut(s) 200, 319, 436
TaiI ACGT 1 cut(s) 352
TasI AATT 2 cut(s) 4, 265
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 2 cut(s) 178, 252
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 2 cut(s) 79, 349
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 79, 349
XapI RAATTY 1 cut(s) 4
XspI CTAG 1 cut(s) 445
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.