MD07G1108700.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003149
Physical Location & Seq
Forward (+)
12474617 .. 12476179
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1108700.v1.1.491

Sequence Viewer

Length: 435 bp
ATGAATTTTGCAACATCAATATGCAGTAGGTTGGGTCTGAAGAACATTGTTACGAATACTCCCGTATACAGCAGTGCTTCTGATGTCACGGCAGAAGGATTGAGTTTGACCTTTAGGCGCTGGGCTACTAAAAAATCTGCTGGATCCACCAAAAATGGTCGAGATTCAAATCCCAAATTTCTTGGAGTGAAGAAATTTGGCGGAGAGAGAGTGATACCTGGAAACATCATAGTTCGCCAAAGAGGCACCCGTTTTCATCCTGGAAATTATGTTGGTTTAGGGAAGGATCACACCCTTTTTGCTCTGAAAGAAGGCTCTGTTAAGTTTGAACACCACAAGCTCAGCGGACGCAAGTGGGTGCATATTGTGCCAAAGGAAGGTCACGTGCTTCACCCCGTCTATGCAAATGGTGCGACTGCGCCATCGACAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

145

Amino Acids

15.67

Weight (kDa)

10.45

Isoelectric Point (pI)

25.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 121 6.2e-37 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 66
AciI CCGC 2 cut(s) 201, 345
AclWI GGATC 3 cut(s) 138, 151, 294
AcsI RAATTY 3 cut(s) 4, 176, 194
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 168, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
AlwI GGATC 3 cut(s) 138, 151, 294
ApoI RAATTY 3 cut(s) 4, 176, 194
AspLEI GCGC 2 cut(s) 120, 421
AsuHPI GGTGA 1 cut(s) 383
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 245
BbrPI CACGTG 1 cut(s) 385
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 2 cut(s) 219, 261
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 243
BlpI GCTNAGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 2 cut(s) 145, 247
BmrFI CCNGG 2 cut(s) 219, 261
Bpu1102I GCTNAGC 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 168
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 355
BseYI CCCAGC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 377
Bsp143I GATC 2 cut(s) 143, 286
Bsp1720I GCTNAGC 1 cut(s) 341
BspACI CCGC 2 cut(s) 201, 345
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 145, 247
BspPI GGATC 3 cut(s) 138, 151, 294
BspT107I GGYRCC 1 cut(s) 245
BssMI GATC 2 cut(s) 143, 286
BssNAI GTATAC 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 219, 261
BstAPI GCANNNNNTGC 2 cut(s) 367, 410
BstBAI YACGTR 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 421
BstKTI GATC 2 cut(s) 146, 289
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 3 cut(s) 243, 367, 410
BstNI CCWGG 2 cut(s) 219, 261
BstSCI CCNGG 2 cut(s) 217, 259
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BstZ17I GTATAC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
CfoI GCGC 2 cut(s) 120, 421
CseI GACGC 1 cut(s) 357
CviJI RGCY 3 cut(s) 125, 315, 340
CviKI_1 RGCY 3 cut(s) 125, 315, 340
DdeI CTNAG 1 cut(s) 341
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
EciI GGCGGA 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 385
EcoRII CCWGG 2 cut(s) 217, 259
FaiI YATR 6 cut(s) 22, 67, 230, 270, 363, 402
FblI GTMKAC 1 cut(s) 66
FokI GGATG 1 cut(s) 243
GlaI GCGC 2 cut(s) 119, 420
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HgaI GACGC 1 cut(s) 357
HhaI GCGC 2 cut(s) 120, 421
Hin6I GCGC 2 cut(s) 118, 419
HinP1I GCGC 2 cut(s) 118, 419
HinfI GANTC 1 cut(s) 164
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 39, 82, 306
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 89, 277, 305, 371
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 4 cut(s) 11, 24, 361, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 367, 410
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 384
HspAI GCGC 2 cut(s) 118, 419
Kzo9I GATC 2 cut(s) 143, 286
LpnPI CCDG 6 cut(s) 106, 126, 204, 231, 246, 273
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 3 cut(s) 49, 85, 380
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 2 cut(s) 52, 202
MflI RGATCY 1 cut(s) 143
MluCI AATT 4 cut(s) 4, 176, 194, 265
MnlI CCTC 1 cut(s) 236
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 219, 261
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 3 cut(s) 243, 367, 410
NdeII GATC 2 cut(s) 143, 286
NlaIV GGNNCC 2 cut(s) 145, 247
NmuCI GTSAC 2 cut(s) 85, 380
PfeI GAWTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 259
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 217, 259
PspCI CACGTG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 2 cut(s) 145, 247
PsuI RGATCY 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 321
Sau3AI GATC 2 cut(s) 143, 286
ScrFI CCNGG 2 cut(s) 219, 261
SetI ASST 6 cut(s) 32, 113, 220, 342, 382, 387
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 4 cut(s) 4, 176, 194, 265
SsiI CCGC 2 cut(s) 201, 345
StyD4I CCNGG 2 cut(s) 217, 259
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 160, 425
TasI AATT 4 cut(s) 4, 176, 194, 265
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 85, 380
Tsp45I GTSAC 2 cut(s) 85, 380
TspDTI ATGAA 2 cut(s) 17, 245
TspRI CASTG 1 cut(s) 79
XapI RAATTY 3 cut(s) 4, 176, 194
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.