Rmu_sc0001971.1_g000011

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001971.1
OG0003149
Physical Location & Seq
Reverse (-)
52333 .. 52949
617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001971.1_g000011.1.cds

Sequence Viewer

Length: 423 bp
ctgtgccaaagattgaatataaagcatctcgttaccagtactcctgtttacagcagtgcctctgatgtcacgggggaaggattgagtttgatgttcaggcgctgggctactaaaaaacaagctggatcctcaaagaatgggcgagactctttacctaagaatcttggagtgaagaaatttggtggagagagagtgatacctggaaacatcattgttcgccagaggggcacccgattccatcctggaaattatgttgggttagggaaggatcacaccctttttgctctgaaagaaggcaccgtaaagtttgaaaagcacaagctcagtggacgtaagtgggtgcatgtagagcccaaagaaggccatgtgcttcaccctatatatgcgaatgttgcagcagctgagacggtgaagacagtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

140

Amino Acids

15.47

Weight (kDa)

10.29

Isoelectric Point (pI)

24.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 227, 296
AclWI GGATC 3 cut(s) 120, 133, 276
AcsI RAATTY 1 cut(s) 176
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 2 cut(s) 16, 311
AjnI CCWGG 2 cut(s) 199, 241
AluBI AGCT 3 cut(s) 122, 322, 401
AluI AGCT 3 cut(s) 122, 322, 401
Alw26I GTCTC 2 cut(s) 138, 398
AlwI GGATC 3 cut(s) 120, 133, 276
AlwNI CAGNNNCTG 2 cut(s) 102, 401
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 2 cut(s) 395, 398
ApoI RAATTY 1 cut(s) 176
AspLEI GCGC 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 365, 421
BaeGI GKGCMC 1 cut(s) 230
BamHI GGATCC 1 cut(s) 125
BanI GGYRCC 2 cut(s) 227, 296
BanII GRGCYC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 407, 410
BccI CCATC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 201, 243
BcoDI GTCTC 2 cut(s) 138, 398
BfoI RGCGCY 1 cut(s) 103
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 2 cut(s) 396, 399
BlsI GCNGC 2 cut(s) 397, 400
BmcAI AGTACT 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 201, 243
BmiI GGNNCC 3 cut(s) 127, 229, 298
BmrFI CCNGG 2 cut(s) 201, 243
BmsI GCATC 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 419
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 36
BseBI CCWGG 2 cut(s) 201, 243
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 2 cut(s) 337, 393
BseNI ACTGG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 2 cut(s) 407, 410
BseYI CCCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 363
BshNI GGYRCC 2 cut(s) 227, 296
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 2 cut(s) 138, 398
BsmBI CGTCTC 1 cut(s) 398
BsnI GGCC 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 230, 354
Bsp143I GATC 2 cut(s) 125, 268
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 2 cut(s) 336, 394
BspLI GGNNCC 3 cut(s) 127, 229, 298
BspPI GGATC 3 cut(s) 120, 133, 276
BspT107I GGYRCC 2 cut(s) 227, 296
BsrI ACTGG 1 cut(s) 36
BssMI GATC 2 cut(s) 125, 268
Bst2UI CCWGG 2 cut(s) 201, 243
Bst4CI ACNGT 3 cut(s) 301, 409, 418
BstDEI CTNAG 3 cut(s) 156, 323, 402
BstF5I GGATG 1 cut(s) 238
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 2 cut(s) 128, 271
BstMAI GTCTC 2 cut(s) 138, 398
BstMBI GATC 2 cut(s) 125, 268
BstMWI GCNNNNNNNGC 3 cut(s) 225, 349, 392
BstNI CCWGG 2 cut(s) 201, 243
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 199, 241
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 407, 410
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BsuRI GGCC 1 cut(s) 363
BtsCI GGATG 1 cut(s) 238
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 331
CaiI CAGNNNCTG 2 cut(s) 102, 401
CfoI GCGC 1 cut(s) 102
Csp6I GTAC 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 2 cut(s) 344, 365
CviJI RGCY 6 cut(s) 107, 122, 322, 352, 363, 401
CviKI_1 RGCY 6 cut(s) 107, 122, 322, 352, 363, 401
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 3 cut(s) 156, 323, 402
DpnI GATC 2 cut(s) 127, 270
DpnII GATC 2 cut(s) 125, 268
Eco24I GRGCYC 1 cut(s) 354
EcoRII CCWGG 2 cut(s) 199, 241
EcoT38I GRGCYC 1 cut(s) 354
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 2 cut(s) 347, 368
FaiI YATR 7 cut(s) 20, 252, 345, 366, 380, 382, 384
FatI CATG 2 cut(s) 343, 364
Fnu4HI GCNGC 2 cut(s) 396, 399
FokI GGATG 1 cut(s) 225
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 2 cut(s) 396, 399
GlaI GCGC 1 cut(s) 101
GluI GCNGC 2 cut(s) 396, 399
GsaI CCCAGC 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 1 cut(s) 363
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 347, 368
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 3 cut(s) 146, 160, 234
HphI GGTGA 2 cut(s) 365, 421
Hpy166II GTNNAC 2 cut(s) 49, 329
Hpy188I TCNGA 3 cut(s) 64, 288, 422
Hpy8I GTNNAC 2 cut(s) 49, 329
HpyAV CCTTC 4 cut(s) 71, 259, 287, 353
HpyCH4III ACNGT 3 cut(s) 301, 409, 418
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 2 cut(s) 343, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 225, 349, 392
HpyF3I CTNAG 3 cut(s) 156, 323, 402
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 2 cut(s) 347, 368
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 125, 268
Lsp1109I GCAGC 2 cut(s) 407, 410
LweI GCATC 1 cut(s) 34
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 2 cut(s) 31, 67
MalI GATC 2 cut(s) 127, 270
MboI GATC 2 cut(s) 125, 268
MboII GAAGA 1 cut(s) 184
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 2 cut(s) 230, 354
MluCI AATT 2 cut(s) 176, 247
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 3 cut(s) 70, 139, 216
MspA1I CMGCKG 1 cut(s) 401
MspR9I CCNGG 2 cut(s) 201, 243
MvaI CCWGG 2 cut(s) 201, 243
MwoI GCNNNNNNNGC 3 cut(s) 225, 349, 392
NdeII GATC 2 cut(s) 125, 268
NlaIII CATG 2 cut(s) 347, 368
NlaIV GGNNCC 3 cut(s) 127, 229, 298
NmuCI GTSAC 1 cut(s) 67
NspI RCATGY 1 cut(s) 347
PfeI GAWTC 2 cut(s) 160, 234
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 2 cut(s) 397, 400
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 199, 241
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 2 cut(s) 199, 241
PspN4I GGNNCC 3 cut(s) 127, 229, 298
PstNI CAGNNNCTG 2 cut(s) 102, 401
PsuI RGATCY 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SatI GCNGC 2 cut(s) 396, 399
Sau3AI GATC 2 cut(s) 125, 268
ScaI AGTACT 1 cut(s) 40
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 201, 243
SduI GDGCHC 2 cut(s) 230, 354
SetI ASST 6 cut(s) 124, 157, 202, 324, 334, 403
SfaNI GCATC 1 cut(s) 34
Sse9I AATT 2 cut(s) 176, 247
StyD4I CCNGG 2 cut(s) 199, 241
TaaI ACNGT 3 cut(s) 301, 409, 418
TaiI ACGT 1 cut(s) 334
TasI AATT 2 cut(s) 176, 247
TatI WGTACW 1 cut(s) 38
TfiI GAWTC 2 cut(s) 160, 234
TscAI CASTG 2 cut(s) 61, 331
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 395, 398
Tsp45I GTSAC 1 cut(s) 67
TspRI CASTG 2 cut(s) 61, 331
XapI RAATTY 1 cut(s) 176
XceI RCATGY 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.