Rroxscaffold_176G00431670

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0003149
Physical Location & Seq
Forward (+)
1532522 .. 1533144
623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431670.1

Sequence Viewer

Length: 441 bp
ATGAATTTTGGAACATCACTATGCCAAAGATTGAATATAAAGCATCTGGTTACCAGTACTCCTGTTTACAGCAGTGCTTCTGATGTCACAGGAGAAGGATTGAGTTTGATATTCAGGCGCTGGGCTACTAAAAAAAATCTTGGATCCTCAAAGAATGGGCGAGACTCTTTACCTAAGAATCTTGGAGTGAAGAAATTCGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAAGATCACACTCTTTTTGCTCTGAAAGAAGGCACCGTCAAGTTTGAAAAGCACAAGCTCACTGGACGTAAGTGGGTGCATGTAGAGCCCAAAGACGGTCATGTGCTTCACCCTATATACGCGAATGCTGCAGCAGCTGAGACGGTGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

146

Amino Acids

16.09

Weight (kDa)

10.29

Isoelectric Point (pI)

20.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 122 4.4e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 314
AccII CGCG 1 cut(s) 404
AclWI GGATC 2 cut(s) 138, 151
AcsI RAATTY 2 cut(s) 4, 194
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 34, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 2 cut(s) 340, 419
AluI AGCT 2 cut(s) 340, 419
Alw26I GTCTC 2 cut(s) 156, 416
AlwI GGATC 2 cut(s) 138, 151
AlwNI CAGNNNCTG 2 cut(s) 120, 419
ApeKI GCWGC 3 cut(s) 410, 413, 416
ApoI RAATTY 2 cut(s) 4, 194
Asp700I GAANNNNTTC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 383, 439
BaeGI GKGCMC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 2 cut(s) 245, 314
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 397, 425, 428
BccI CCATC 1 cut(s) 264
BciT130I CCWGG 2 cut(s) 219, 261
BcoDI GTCTC 2 cut(s) 156, 416
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 3 cut(s) 411, 414, 417
BlsI GCNGC 3 cut(s) 412, 415, 418
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 3 cut(s) 145, 247, 316
BmrFI CCNGG 2 cut(s) 219, 261
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 437
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 54, 349
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 2 cut(s) 54, 349
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 3 cut(s) 397, 425, 428
BseYI CCCAGC 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 404
BshNI GGYRCC 2 cut(s) 245, 314
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 2 cut(s) 156, 416
BsmBI CGTCTC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 248, 372
Bsp143I GATC 2 cut(s) 143, 286
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 3 cut(s) 145, 247, 316
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 2 cut(s) 138, 151
BspT107I GGYRCC 2 cut(s) 245, 314
BsrI ACTGG 2 cut(s) 54, 349
BssMI GATC 2 cut(s) 143, 286
Bst2UI CCWGG 2 cut(s) 219, 261
Bst4CI ACNGT 4 cut(s) 319, 380, 427, 436
BstDEI CTNAG 2 cut(s) 174, 420
BstEII GGTNACC 1 cut(s) 49
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 2 cut(s) 156, 416
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
BstNI CCWGG 2 cut(s) 219, 261
BstNSI RCATGY 1 cut(s) 365
BstPI GGTNACC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 217, 259
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 248
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 3 cut(s) 397, 425, 428
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 342
CaiI CAGNNNCTG 2 cut(s) 120, 419
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 57
CviAII CATG 2 cut(s) 362, 383
CviJI RGCY 4 cut(s) 125, 340, 370, 419
CviKI_1 RGCY 4 cut(s) 125, 340, 370, 419
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 2 cut(s) 174, 420
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
Eco24I GRGCYC 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 217, 259
EcoT38I GRGCYC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 2 cut(s) 365, 386
FaiI YATR 7 cut(s) 22, 38, 270, 363, 384, 398, 400
FatI CATG 2 cut(s) 361, 382
Fnu4HI GCNGC 3 cut(s) 411, 414, 417
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 411, 414, 417
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 411, 414, 417
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HhaI GCGC 1 cut(s) 120
Hin1II CATG 2 cut(s) 365, 386
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 164, 178, 252
HphI GGTGA 2 cut(s) 383, 439
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 82, 306, 440
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 89, 305
HpyCH4III ACNGT 4 cut(s) 319, 380, 427, 436
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 361, 413
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
HpyF3I CTNAG 2 cut(s) 174, 420
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 365, 386
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
Lsp1109I GCAGC 3 cut(s) 397, 425, 428
LweI GCATC 1 cut(s) 52
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 49, 85
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 202
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 248, 372
MluCI AATT 3 cut(s) 4, 194, 265
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 2 cut(s) 157, 234
MroXI GAANNNNTTC 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 219, 261
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 2 cut(s) 219, 261
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 2 cut(s) 365, 386
NlaIV GGNNCC 3 cut(s) 145, 247, 316
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 1 cut(s) 365
PctI GAATGC 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 178, 252
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 3 cut(s) 412, 415, 418
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 217, 259
PspEI GGTNACC 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 3 cut(s) 145, 247, 316
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 2 cut(s) 120, 419
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 19
SatI GCNGC 3 cut(s) 411, 414, 417
Sau3AI GATC 2 cut(s) 143, 286
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 2 cut(s) 248, 372
SetI ASST 5 cut(s) 175, 220, 342, 352, 421
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 3 cut(s) 4, 194, 265
StyD4I CCNGG 2 cut(s) 217, 259
TaaI ACNGT 4 cut(s) 319, 380, 427, 436
TaiI ACGT 1 cut(s) 352
TasI AATT 3 cut(s) 4, 194, 265
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 2 cut(s) 178, 252
TscAI CASTG 2 cut(s) 79, 349
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 3 cut(s) 410, 413, 416
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 79, 349
XapI RAATTY 2 cut(s) 4, 194
XceI RCATGY 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.