Rroxscaffold_3G00237480

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003149
Physical Location & Seq
Reverse (-)
25128477 .. 25130420
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237480.1

Sequence Viewer

Length: 441 bp
ATGAATTTCGGAACATCACTATGCCAAAGATTGAATATAAAGCATCTCGTTACCAGTACTCCTGTTTACAGCAGTGCCTCTGATGTCACAGGGGAAGGATTGAGTTTGATGTTCAGGCGCTGGGCTACTAAAAAACAAGCTGGATCCTCAAAGAATGGGCGAGACTCGTTACCTAAGAATCTTGGAGTGAAGAAATTTGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAGGATCACACTCTTTTTGCTCTGAAAGAAGGCACCGTAAAGTTTGAAAAGCACAAGCTCAGTGGACGTAAGTGGGTGCATGTAGAGCCCAAAGAAGGTCATGTGCTTCACCCTATATATGCGAATGTTGCAGCCGCTGAGATGGTGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

146

Amino Acids

16.14

Weight (kDa)

10.29

Isoelectric Point (pI)

22.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 122 1.8e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 314
AciI CCGC 1 cut(s) 417
AclWI GGATC 3 cut(s) 138, 151, 294
AcsI RAATTY 2 cut(s) 4, 194
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 34, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 2 cut(s) 140, 340
AluI AGCT 2 cut(s) 140, 340
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 3 cut(s) 138, 151, 294
AlwNI CAGNNNCTG 2 cut(s) 120, 419
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 2 cut(s) 4, 194
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 383, 439
BaeGI GKGCMC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 2 cut(s) 245, 314
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 1 cut(s) 425
BccI CCATC 2 cut(s) 264, 418
BciT130I CCWGG 2 cut(s) 219, 261
BcoDI GTCTC 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 2 cut(s) 414, 417
BlsI GCNGC 2 cut(s) 415, 418
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 3 cut(s) 145, 247, 316
BmrFI CCNGG 2 cut(s) 219, 261
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 437
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 355, 411
BseNI ACTGG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 1 cut(s) 425
BseYI CCCAGC 1 cut(s) 120
BshNI GGYRCC 2 cut(s) 245, 314
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 248, 372
Bsp143I GATC 2 cut(s) 143, 286
BspACI CCGC 1 cut(s) 417
BspCNI CTCAG 2 cut(s) 354, 412
BspLI GGNNCC 3 cut(s) 145, 247, 316
BspPI GGATC 3 cut(s) 138, 151, 294
BspT107I GGYRCC 2 cut(s) 245, 314
BsrI ACTGG 1 cut(s) 54
BssMI GATC 2 cut(s) 143, 286
Bst2UI CCWGG 2 cut(s) 219, 261
Bst4CI ACNGT 2 cut(s) 319, 436
BstDEI CTNAG 3 cut(s) 174, 341, 420
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 3 cut(s) 243, 367, 410
BstNI CCWGG 2 cut(s) 219, 261
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 217, 259
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 425
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 349
CaiI CAGNNNCTG 2 cut(s) 120, 419
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 2 cut(s) 362, 383
CviJI RGCY 5 cut(s) 125, 140, 340, 370, 416
CviKI_1 RGCY 5 cut(s) 125, 140, 340, 370, 416
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 174, 341, 420
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
Eco24I GRGCYC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 217, 259
EcoT38I GRGCYC 1 cut(s) 372
FaeI CATG 2 cut(s) 365, 386
FaiI YATR 8 cut(s) 22, 38, 270, 363, 384, 398, 400, 402
FatI CATG 2 cut(s) 361, 382
Fnu4HI GCNGC 2 cut(s) 414, 417
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 414, 417
GlaI GCGC 1 cut(s) 119
GluI GCNGC 2 cut(s) 414, 417
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HhaI GCGC 1 cut(s) 120
Hin1II CATG 2 cut(s) 365, 386
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 164, 178, 252
HphI GGTGA 2 cut(s) 383, 439
Hpy166II GTNNAC 2 cut(s) 67, 347
Hpy188I TCNGA 4 cut(s) 11, 82, 306, 440
Hpy8I GTNNAC 2 cut(s) 67, 347
HpyAV CCTTC 4 cut(s) 89, 277, 305, 371
HpyCH4III ACNGT 2 cut(s) 319, 436
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 361, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 367, 410
HpyF3I CTNAG 3 cut(s) 174, 341, 420
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 365, 386
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 1 cut(s) 52
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 3 cut(s) 49, 85, 168
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 202
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 248, 372
MluCI AATT 3 cut(s) 4, 194, 265
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 3 cut(s) 88, 157, 234
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 219, 261
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 3 cut(s) 243, 367, 410
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 2 cut(s) 365, 386
NlaIV GGNNCC 3 cut(s) 145, 247, 316
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 1 cut(s) 365
PfeI GAWTC 2 cut(s) 178, 252
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 2 cut(s) 415, 418
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 217, 259
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 3 cut(s) 145, 247, 316
PstNI CAGNNNCTG 2 cut(s) 120, 419
PsuI RGATCY 1 cut(s) 143
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 19
SatI GCNGC 2 cut(s) 414, 417
Sau3AI GATC 2 cut(s) 143, 286
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 2 cut(s) 248, 372
SetI ASST 6 cut(s) 142, 175, 220, 342, 352, 382
SfaNI GCATC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 3 cut(s) 4, 194, 265
SsiI CCGC 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 217, 259
TaaI ACNGT 2 cut(s) 319, 436
TaiI ACGT 1 cut(s) 352
TasI AATT 3 cut(s) 4, 194, 265
TatI WGTACW 1 cut(s) 56
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 178, 252
TscAI CASTG 2 cut(s) 79, 349
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 79, 349
XapI RAATTY 2 cut(s) 4, 194
XceI RCATGY 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.