MD10G1076100.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003149
Physical Location & Seq
Reverse (-)
10855462 .. 10858128
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1076100.v1.1.491

Sequence Viewer

Length: 435 bp
ATGAATTTTGCAACATCAATATGCAGTAGGTTGGGTCTTAAGAACATTGTTACGAATACTCCCGTATACAGCAGTGCTTCCGATGTCACAGCGGAAGGATTGAGTTTGACCTTTCGGCGCTGGGCTACTAAAAAATCTGCTGGATCCACCAAAAATGGTCGAGATTCAAATCCCAAATTTCTTGGAGTGAAGAAATTTGGCGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAAAGAGGCACCCGTTTTCATCCTGGAAATTATGTTGGTTTAGGGAAGGATCACACCCTTTTTGCTCTGAAAGAAGGCTCTGTTAAGTTTGAACACCACAAGCTCAGCGGACGCAAGTGGGTGCATATCGTGCCCAAGGAAGGTCACATGCTTCACCCTGTCTATGCAAATGGTGGGACTGCACCATCGACAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

145

Amino Acids

15.69

Weight (kDa)

10.45

Isoelectric Point (pI)

25.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 121 6.2e-37 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 66
AciI CCGC 3 cut(s) 92, 201, 345
AclWI GGATC 3 cut(s) 138, 151, 294
AcsI RAATTY 3 cut(s) 4, 176, 194
AfiI CCNNNNNNNGG 1 cut(s) 377
AflII CTTAAG 1 cut(s) 38
AgsI TTSAA 2 cut(s) 168, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
AlwI GGATC 3 cut(s) 138, 151, 294
ApoI RAATTY 3 cut(s) 4, 176, 194
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 372
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 245
BccI CCATC 1 cut(s) 430
BciT130I CCWGG 2 cut(s) 219, 261
BfoI RGCGCY 1 cut(s) 121
BfrI CTTAAG 1 cut(s) 38
BglI GCCNNNNNGGC 1 cut(s) 243
BlpI GCTNAGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 2 cut(s) 145, 247
BmrFI CCNGG 2 cut(s) 219, 261
Bpu1102I GCTNAGC 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 2 cut(s) 219, 261
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 372
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 402
BshNI GGYRCC 1 cut(s) 245
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 2 cut(s) 143, 286
Bsp1720I GCTNAGC 1 cut(s) 341
BspACI CCGC 3 cut(s) 92, 201, 345
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 145, 247
BspPI GGATC 3 cut(s) 138, 151, 294
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 2 cut(s) 143, 286
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 372
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 219, 261
BstAFI CTTAAG 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 2 cut(s) 243, 367
BstNI CCWGG 2 cut(s) 219, 261
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 217, 259
BstSLI GKGCMC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BstZ17I GTATAC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
CfoI GCGC 1 cut(s) 120
CseI GACGC 1 cut(s) 357
CviAII CATG 1 cut(s) 385
CviJI RGCY 3 cut(s) 125, 315, 340
CviKI_1 RGCY 3 cut(s) 125, 315, 340
DdeI CTNAG 1 cut(s) 341
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 217, 259
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 1 cut(s) 388
FaiI YATR 6 cut(s) 22, 67, 270, 363, 386, 402
FaqI GGGAC 1 cut(s) 427
FatI CATG 1 cut(s) 384
FblI GTMKAC 1 cut(s) 66
FokI GGATG 1 cut(s) 243
GlaI GCGC 1 cut(s) 119
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HgaI GACGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 120
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 1 cut(s) 164
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 82, 306
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 89, 277, 305, 371
HpyCH4V TGCA 5 cut(s) 11, 24, 361, 404, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 367
HpyF3I CTNAG 1 cut(s) 341
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
LpnPI CCDG 7 cut(s) 106, 126, 204, 231, 246, 273, 408
MaeIII GTNAC 3 cut(s) 49, 85, 380
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 202
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 4 cut(s) 4, 176, 194, 265
MnlI CCTC 1 cut(s) 236
MseI TTAA 2 cut(s) 39, 321
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 2 cut(s) 92, 345
MspCI CTTAAG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 219, 261
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 2 cut(s) 243, 367
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 145, 247
NmuCI GTSAC 2 cut(s) 85, 380
NspI RCATGY 1 cut(s) 388
PfeI GAWTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 259
Psp6I CCWGG 2 cut(s) 217, 259
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 2 cut(s) 145, 247
PsuI RGATCY 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 2 cut(s) 39, 321
Sau3AI GATC 2 cut(s) 143, 286
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 1 cut(s) 372
SetI ASST 5 cut(s) 32, 113, 220, 342, 382
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 4 cut(s) 4, 176, 194, 265
SsiI CCGC 3 cut(s) 92, 201, 345
StyD4I CCNGG 2 cut(s) 217, 259
StyI CCWWGG 1 cut(s) 372
TaqI TCGA 2 cut(s) 160, 425
TasI AATT 4 cut(s) 4, 176, 194, 265
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 39, 321
Tru9I TTAA 2 cut(s) 39, 321
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 85, 380
Tsp45I GTSAC 2 cut(s) 85, 380
TspDTI ATGAA 2 cut(s) 17, 245
TspRI CASTG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 38
XapI RAATTY 3 cut(s) 4, 176, 194
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.