Rh7BG336300

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003149
Physical Location & Seq
Forward (+)
35043001 .. 35044860
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG336300.1

Sequence Viewer

Length: 426 bp
ATGTTTCGTGAACTCATTATATTGCTGCATCTAACCTCTACTGTTTTACTTGGTGCACTAATTGCAGATGTCACGGGGGAAGGATTGAGTTTGATGTTCAGGCGCTGGGCTACTAAAAAACAAGCTGGATCCTCAAAGAATGGGCGAGACTCTTTACCTAAGAATCTTGGAGTGAAGAAATTCGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAAGATCACACTCTTTTTGCTCTGAAAGAAGGCACCGTAAAGTTTGAAAAGCACAAGCTCAGTGGACGTAAGTGGGTGCATGTAGAGCCCAAAGAAGGTCATGTGCTTCACCCTATATATGCGAATGCTGCAGCAGCTGAGATGGTGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

141

Amino Acids

15.59

Weight (kDa)

10.32

Isoelectric Point (pI)

21.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 37 - 117 1.6e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 230, 299
AclWI GGATC 2 cut(s) 123, 136
AcsI RAATTY 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 2 cut(s) 202, 244
AluBI AGCT 3 cut(s) 125, 325, 404
AluI AGCT 3 cut(s) 125, 325, 404
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 141
Alw44I GTGCAC 1 cut(s) 54
AlwI GGATC 2 cut(s) 123, 136
AlwNI CAGNNNCTG 2 cut(s) 105, 404
ApaLI GTGCAC 1 cut(s) 54
ApeKI GCWGC 4 cut(s) 25, 395, 398, 401
ApoI RAATTY 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 368, 424
BaeGI GKGCMC 2 cut(s) 58, 233
BamHI GGATCC 1 cut(s) 128
BanI GGYRCC 2 cut(s) 230, 299
BanII GRGCYC 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 4 cut(s) 12, 382, 410, 413
BccI CCATC 2 cut(s) 249, 403
BciT130I CCWGG 2 cut(s) 204, 246
BcoDI GTCTC 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 106
BglI GCCNNNNNGGC 1 cut(s) 228
BisI GCNGC 4 cut(s) 26, 396, 399, 402
BlsI GCNGC 4 cut(s) 27, 397, 400, 403
Bme1390I CCNGG 2 cut(s) 204, 246
BmiI GGNNCC 3 cut(s) 130, 232, 301
BmrFI CCNGG 2 cut(s) 204, 246
BmsI GCATC 1 cut(s) 37
BpiI GAAGAC 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 362
BseBI CCWGG 2 cut(s) 204, 246
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 2 cut(s) 340, 396
BseSI GKGCMC 2 cut(s) 58, 233
BseXI GCAGC 4 cut(s) 12, 382, 410, 413
BseYI CCCAGC 1 cut(s) 105
BshNI GGYRCC 2 cut(s) 230, 299
BsiHKAI GWGCWC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 397
Bsp1286I GDGCHC 3 cut(s) 58, 233, 357
Bsp143I GATC 2 cut(s) 128, 271
BspCNI CTCAG 2 cut(s) 339, 397
BspLI GGNNCC 3 cut(s) 130, 232, 301
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 2 cut(s) 123, 136
BspT107I GGYRCC 2 cut(s) 230, 299
BssMI GATC 2 cut(s) 128, 271
Bst2UI CCWGG 2 cut(s) 204, 246
Bst4CI ACNGT 3 cut(s) 43, 304, 421
BstAPI GCANNNNNTGC 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 159, 326, 405
BstF5I GGATG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 131, 274
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 2 cut(s) 128, 271
BstMWI GCNNNNNNNGC 5 cut(s) 62, 228, 352, 395, 401
BstNI CCWGG 2 cut(s) 204, 246
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 202, 244
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 2 cut(s) 58, 233
BstV1I GCAGC 4 cut(s) 12, 382, 410, 413
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 334
CaiI CAGNNNCTG 2 cut(s) 105, 404
CfoI GCGC 1 cut(s) 105
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviAII CATG 2 cut(s) 347, 368
CviJI RGCY 5 cut(s) 110, 125, 325, 355, 404
CviKI_1 RGCY 5 cut(s) 110, 125, 325, 355, 404
DdeI CTNAG 3 cut(s) 159, 326, 405
DpnI GATC 2 cut(s) 130, 273
DpnII GATC 2 cut(s) 128, 271
Eco24I GRGCYC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 202, 244
EcoT38I GRGCYC 1 cut(s) 357
FaeI CATG 2 cut(s) 350, 371
FaiI YATR 7 cut(s) 20, 255, 348, 369, 383, 385, 387
FatI CATG 2 cut(s) 346, 367
Fnu4HI GCNGC 4 cut(s) 26, 396, 399, 402
FokI GGATG 1 cut(s) 228
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 4 cut(s) 26, 396, 399, 402
GlaI GCGC 1 cut(s) 104
GluI GCNGC 4 cut(s) 26, 396, 399, 402
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 106
HhaI GCGC 1 cut(s) 105
Hin1II CATG 2 cut(s) 350, 371
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 3 cut(s) 149, 163, 237
HphI GGTGA 2 cut(s) 368, 424
Hpy166II GTNNAC 3 cut(s) 11, 56, 332
Hpy188I TCNGA 2 cut(s) 291, 425
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 3 cut(s) 11, 56, 332
HpyAV CCTTC 3 cut(s) 74, 290, 356
HpyCH4III ACNGT 3 cut(s) 43, 304, 421
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 28, 56, 65, 346, 398
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 228, 352, 395, 401
HpyF3I CTNAG 3 cut(s) 159, 326, 405
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 2 cut(s) 350, 371
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 128, 271
LpnPI CCDG 8 cut(s) 85, 91, 111, 189, 216, 231, 236, 258
Lsp1109I GCAGC 4 cut(s) 12, 382, 410, 413
LweI GCATC 1 cut(s) 37
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 130, 273
MboI GATC 2 cut(s) 128, 271
MboII GAAGA 1 cut(s) 187
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 58, 233, 357
MluCI AATT 3 cut(s) 60, 179, 250
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 3 cut(s) 46, 142, 219
MroXI GAANNNNTTC 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 404
MspR9I CCNGG 2 cut(s) 204, 246
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 2 cut(s) 204, 246
MwoI GCNNNNNNNGC 5 cut(s) 62, 228, 352, 395, 401
NdeII GATC 2 cut(s) 128, 271
NlaIII CATG 2 cut(s) 350, 371
NlaIV GGNNCC 3 cut(s) 130, 232, 301
NmuCI GTSAC 1 cut(s) 70
NspI RCATGY 1 cut(s) 350
PctI GAATGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 163, 237
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 4 cut(s) 27, 397, 400, 403
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 202, 244
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 2 cut(s) 202, 244
PspN4I GGNNCC 3 cut(s) 130, 232, 301
PstI CTGCAG 1 cut(s) 400
PstNI CAGNNNCTG 2 cut(s) 105, 404
PsuI RGATCY 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 404
SatI GCNGC 4 cut(s) 26, 396, 399, 402
Sau3AI GATC 2 cut(s) 128, 271
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 204, 246
SduI GDGCHC 3 cut(s) 58, 233, 357
SetI ASST 8 cut(s) 38, 127, 160, 205, 327, 337, 367, 406
SfaNI GCATC 1 cut(s) 37
SfcI CTRYAG 1 cut(s) 396
Sse9I AATT 3 cut(s) 60, 179, 250
StyD4I CCNGG 2 cut(s) 202, 244
TaaI ACNGT 3 cut(s) 43, 304, 421
TaiI ACGT 1 cut(s) 337
TasI AATT 3 cut(s) 60, 179, 250
TfiI GAWTC 2 cut(s) 163, 237
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 4 cut(s) 25, 395, 398, 401
Tsp45I GTSAC 1 cut(s) 70
TspRI CASTG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 54
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.