FvH4_2g08800

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003149
Physical Location & Seq
Forward (+)
7685014 .. 7687387
2374 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08800.t3

Sequence Viewer

Length: 441 bp
ATGAATTTCGGAACTTCACTATGCCAAAGATTGAATATAAAGCATCTAGTTACCAGTACTGCTGTTTACAACAGTGCTTCTGATGTCACAGGAGAGGGATTAAGTTTGATGTTTAGGCGCTGGGCTAGTAAAAGAGCAGCTGGATCCACAAAGAATGGGCGAGACTCTCAACCTAAGAATCTTGGAGTGAAAAAATTTGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGACAGATCACACTCTTTTTGCTCTGAAAGAAGGCACTGTAAAGTTTGAAAAGCACAAGCTCAGTGGACGAAAGTGGGTGCATATAGAGCCCAAAGAAGGTCATGTGCTTCACCCTATATGTGCAAATACTACAGCAGCTGAGACAATGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

147

Amino Acids

16.08

Weight (kDa)

10.29

Isoelectric Point (pI)

24.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 6 - 85 9.8e-37 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AclWI GGATC 2 cut(s) 138, 151
AcsI RAATTY 2 cut(s) 4, 194
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 34, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 3 cut(s) 140, 340, 419
AluI AGCT 3 cut(s) 140, 340, 419
Alw26I GTCTC 2 cut(s) 156, 416
AlwI GGATC 2 cut(s) 138, 151
AlwNI CAGNNNCTG 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 137, 416
ApoI RAATTY 2 cut(s) 4, 194
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 149, 428
BccI CCATC 1 cut(s) 264
BciT130I CCWGG 2 cut(s) 219, 261
BcoDI GTCTC 2 cut(s) 156, 416
BfaI CTAG 2 cut(s) 47, 126
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 2 cut(s) 138, 417
BlsI GCNGC 2 cut(s) 139, 418
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 2 cut(s) 145, 247
BmrFI CCNGG 2 cut(s) 219, 261
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 355, 411
BseNI ACTGG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 2 cut(s) 149, 428
BseYI CCCAGC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 245
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 2 cut(s) 156, 416
BsmFI GGGAC 1 cut(s) 295
Bsp1286I GDGCHC 2 cut(s) 248, 372
Bsp143I GATC 2 cut(s) 143, 286
BspCNI CTCAG 2 cut(s) 354, 412
BspLI GGNNCC 2 cut(s) 145, 247
BspPI GGATC 2 cut(s) 138, 151
BspT107I GGYRCC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 54
BssMI GATC 2 cut(s) 143, 286
Bst2UI CCWGG 2 cut(s) 219, 261
Bst4CI ACNGT 3 cut(s) 74, 319, 436
BstDEI CTNAG 3 cut(s) 174, 341, 420
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 2 cut(s) 156, 416
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 2 cut(s) 243, 367
BstNI CCWGG 2 cut(s) 219, 261
BstSCI CCNGG 2 cut(s) 217, 259
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 149, 428
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 79, 315, 349
CaiI CAGNNNCTG 1 cut(s) 419
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 1 cut(s) 383
CviJI RGCY 5 cut(s) 125, 140, 340, 370, 419
CviKI_1 RGCY 5 cut(s) 125, 140, 340, 370, 419
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 174, 341, 420
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
Eco24I GRGCYC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 217, 259
EcoT38I GRGCYC 1 cut(s) 372
FaeI CATG 1 cut(s) 386
FaiI YATR 8 cut(s) 22, 38, 270, 363, 365, 384, 398, 400
FaqI GGGAC 1 cut(s) 295
FatI CATG 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 138, 417
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 138, 417
FspBI CTAG 2 cut(s) 47, 126
GlaI GCGC 1 cut(s) 119
GluI GCNGC 2 cut(s) 138, 417
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HhaI GCGC 1 cut(s) 120
Hin1II CATG 1 cut(s) 386
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 164, 178, 252
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 2 cut(s) 67, 347
Hpy188I TCNGA 4 cut(s) 11, 82, 306, 440
Hpy8I GTNNAC 2 cut(s) 67, 347
HpyAV CCTTC 2 cut(s) 305, 371
HpyCH4III ACNGT 3 cut(s) 74, 319, 436
HpyCH4V TGCA 2 cut(s) 361, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 367
HpyF3I CTNAG 3 cut(s) 174, 341, 420
Hsp92II CATG 1 cut(s) 386
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
LpnPI CCDG 9 cut(s) 67, 75, 106, 126, 204, 231, 246, 251, 273
Lsp1109I GCAGC 2 cut(s) 149, 428
LweI GCATC 1 cut(s) 52
MaeI CTAG 2 cut(s) 47, 126
MaeIII GTNAC 2 cut(s) 49, 85
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 248, 372
MluCI AATT 3 cut(s) 4, 194, 265
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 2 cut(s) 88, 234
MseI TTAA 1 cut(s) 101
MspA1I CMGCKG 2 cut(s) 140, 419
MspR9I CCNGG 2 cut(s) 219, 261
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 2 cut(s) 243, 367
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 1 cut(s) 386
NlaIV GGNNCC 2 cut(s) 145, 247
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 178, 252
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 2 cut(s) 139, 418
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 217, 259
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 2 cut(s) 145, 247
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 2 cut(s) 140, 419
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 138, 417
Sau3AI GATC 2 cut(s) 143, 286
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 2 cut(s) 248, 372
SetI ASST 6 cut(s) 142, 175, 220, 342, 382, 421
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 411
Sse9I AATT 3 cut(s) 4, 194, 265
SspMI CTAG 2 cut(s) 47, 126
StyD4I CCNGG 2 cut(s) 217, 259
TaaI ACNGT 3 cut(s) 74, 319, 436
TasI AATT 3 cut(s) 4, 194, 265
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 2 cut(s) 178, 252
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 3 cut(s) 79, 322, 349
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 137, 416
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 79, 322, 349
XapI RAATTY 2 cut(s) 4, 194
XspI CTAG 2 cut(s) 47, 126
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.