AT2G19560

PCI domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002448
Physical Location & Seq
Reverse (-)
8465690 .. 8469145
3456 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19560.1

Sequence Viewer

Length: 1242 bp
ATGGCGTACGTTAGTATGGGTGAAGCTCACCGGAGAATCACTGAATACCTAAACCGATTCTGCGACGCCGTTTCGTACCAAGACTCTTCTACACTTTGCCGTCTCCTCTCTTTCTCTTCCAATTCTCCACCTCTTCTCTCACTCGCCGACGCACTCAACGTCTTCCAGGATTCTAGCAGTTTGATTAGACAGTCTGATAGATTCTCTGAATATGGCGAGATTCTGGCTCATGTGTTTCGTTCTCTACAAAGTTACCGAGTTGGGAATCTAGTCGAAGCATACCTTGCTTTCGATAAGTTTGCCAATGCATTTGTTCAGGAGTTTCGTAATTGGGAATCTGCTTGGGCATTGGAAGCTCTGTATGTAGTTTGTTATGAAATTCGAGTTCTTGCGGAAAAGGCTGATAAAGATTTAACTTCTAATGGAAAGTCTCCAGAGAAATTAAAAGCAGCTGGGTCTCTTCTCATGAAAGTTTTTGGAGTTCTTGCTGGGAAAGGTCCAAAACGAGTCGGAGCATTATATGTGACTTGCCAATTGTTCAAGACCTACTTTAAGCTTGGAACCGTCAATCTTTGTCGAAGTGTAATAAGAAGTATCGAAACTGCTCGGATATTTGACTTTGAGGAGTTTCCAAGAAGAGATAAGGTTACATACATGTATTATACCGGCCGATTAGAAGTCTTCAACGAAAATTTTCCTGCTGCTGATACAAAGCTATCATATGCCTTGCAAAATTGCAACCCCAAAAGAGAACGAAATATAAGGATGATATTAAAGTATTTGGTACCGGTGAAGCTTTCTTTAGGAATTATACCAAAAGATGAGCTCCTGCGAAATTATAATCTGCATGAGTACACAAAGATTGTGCAAGCTCTGAGAAAGGGTGATCTAAGGCTTCTCCGGCATGCTCTTCAAGAACATGAAGATCGGTTCTTGAGGTCGGGTGTGTATCTTGTCTTGGAAAAGCTAGAGCTCCAAGTCTACCAGAGACTCATGAAGAAAATTTATATAAACCAGAAGCTGAGTGATCCAGCGAGAGCTCACCAGCTGAAGCTTGAAGGGATTGCCAAAGCACTAAGATGGCTAGATATGGACATGGATCTGGACGAGGTGGAATGTATAATGACGATATTGATATACAAAAACCTTGTGAAAGGTTATTTAGCTCACAAGAGCAAAGTGGTAGTTCTAAGCAAGCAAGATCCTTTCCCTAAACTAAACGGGAAGCCTGTTAGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000956 GO:0000972 GO:0000973 GO:0002376 GO:0002520 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006366 GO:0006368 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007088 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0008298 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010466 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010941 GO:0010951 GO:0010965 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016973 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022622 GO:0023052 GO:0030071 GO:0030162 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031503 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032774 GO:0032870 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035556 GO:0042221 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0045787 GO:0045861 GO:0045893 GO:0045931 GO:0045935 GO:0046483 GO:0046700 GO:0046907 GO:0048364 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048536 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051028 GO:0051128 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051276 GO:0051336 GO:0051346 GO:0051641 GO:0051649 GO:0051716 GO:0051726 GO:0051783 GO:0051983 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0060968 GO:0061013 GO:0065007 GO:0065008 GO:0065009 GO:0070390 GO:0070727 GO:0070887 GO:0071025 GO:0071027 GO:0071028 GO:0071033 GO:0071166 GO:0071310 GO:0071369 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0080090 GO:0090068 GO:0090231 GO:0090232 GO:0090266 GO:0090267 GO:0090304 GO:0097159 GO:0097659 GO:0099402 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1901976 GO:1901978 GO:1901987 GO:1901990 GO:1902099 GO:1902680 GO:1903311 GO:1903504 GO:1903506 GO:1903508 GO:1905818 GO:2000112 GO:2000116 GO:2000117 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCI

Protein Analysis

413

Amino Acids

47.61

Weight (kDa)

9.31

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCI PF01399 304 - 399 2.1e-07 PCI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
Acc65I GGTACC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 981
AciI CCGC 1 cut(s) 392
AclWI GGATC 3 cut(s) 1022, 1107, 1196
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 3 cut(s) 378, 691, 1002
AcuI CTGAAG 1 cut(s) 1070
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 4 cut(s) 8, 77, 786, 854
AflIII ACRYGT 1 cut(s) 654
AgeI ACCGGT 1 cut(s) 787
AgsI TTSAA 4 cut(s) 541, 685, 914, 1058
AjnI CCWGG 1 cut(s) 165
Alw21I GWGCWC 3 cut(s) 828, 975, 1042
Alw26I GTCTC 4 cut(s) 107, 435, 462, 982
AlwI GGATC 3 cut(s) 1022, 1107, 1196
AlwNI CAGNNNCTG 1 cut(s) 1021
AoxI GGCC 1 cut(s) 667
ApeKI GCWGC 2 cut(s) 449, 701
ApoI RAATTY 3 cut(s) 378, 691, 1002
AsiGI ACCGGT 1 cut(s) 787
Asp700I GAANNNNTTC 1 cut(s) 693
Asp718I GGTACC 1 cut(s) 784
AspS9I GGNCC 1 cut(s) 497
AsuHPI GGTGA 5 cut(s) 20, 32, 802, 896, 1034
AvaII GGWCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 3 cut(s) 828, 975, 1042
BbsI GAAGAC 2 cut(s) 154, 673
Bbv12I GWGCWC 3 cut(s) 828, 975, 1042
BbvI GCAGC 2 cut(s) 461, 688
BccI CCATC 1 cut(s) 1074
BceAI ACGGC 2 cut(s) 53, 84
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 4 cut(s) 107, 435, 462, 982
BfaI CTAG 4 cut(s) 174, 269, 968, 1085
BisI GCNGC 2 cut(s) 450, 702
BlsI GCNGC 2 cut(s) 451, 703
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 2 cut(s) 562, 786
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 2 cut(s) 154, 673
BpmI CTGGAG 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 955
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 462
BsaWI WCCGGW 2 cut(s) 30, 787
Bse118I RCCGGY 2 cut(s) 665, 787
BseBI CCWGG 1 cut(s) 167
BseGI GGATG 1 cut(s) 771
BseMII CTCAG 2 cut(s) 866, 1013
BseRI GAGGAG 2 cut(s) 95, 638
BseX3I CGGCCG 1 cut(s) 667
BseXI GCAGC 2 cut(s) 461, 688
BseYI CCCAGC 2 cut(s) 452, 488
Bsh1285I CGRYCG 1 cut(s) 670
BshFI GGCC 1 cut(s) 669
BshNI GGYRCC 1 cut(s) 784
BshTI ACCGGT 1 cut(s) 787
BsiEI CGRYCG 1 cut(s) 670
BsiHKAI GWGCWC 3 cut(s) 828, 975, 1042
BsiSI CCGG 4 cut(s) 31, 666, 788, 901
BsiWI CGTACG 1 cut(s) 6
BsmAI GTCTC 4 cut(s) 107, 435, 462, 982
BsmBI CGTCTC 1 cut(s) 107
BsnI GGCC 1 cut(s) 669
Bso31I GGTCTC 1 cut(s) 462
Bsp1286I GDGCHC 3 cut(s) 828, 975, 1042
Bsp143I GATC 5 cut(s) 886, 925, 1027, 1099, 1201
BspACI CCGC 1 cut(s) 392
BspANI GGCC 1 cut(s) 669
BspCNI CTCAG 2 cut(s) 867, 1014
BspHI TCATGA 2 cut(s) 465, 993
BspLI GGNNCC 2 cut(s) 562, 786
BspPI GGATC 3 cut(s) 1022, 1107, 1196
BspQI GCTCTTC 1 cut(s) 915
BspT107I GGYRCC 1 cut(s) 784
BspTNI GGTCTC 1 cut(s) 462
BsrFI RCCGGY 2 cut(s) 665, 787
BssAI RCCGGY 2 cut(s) 665, 787
BssMI GATC 5 cut(s) 886, 925, 1027, 1099, 1201
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 192, 565
Bst6I CTCTTC 6 cut(s) 91, 121, 138, 465, 631, 915
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 870, 906, 1196
BstDEI CTNAG 5 cut(s) 875, 890, 1022, 1076, 1190
BstF5I GGATG 1 cut(s) 771
BstKTI GATC 5 cut(s) 889, 928, 1030, 1102, 1204
BstMAI GTCTC 4 cut(s) 107, 435, 462, 982
BstMBI GATC 5 cut(s) 886, 925, 1027, 1099, 1201
BstMCI CGRYCG 1 cut(s) 670
BstMWI GCNNNNNNNGC 4 cut(s) 284, 353, 398, 901
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 2 cut(s) 658, 908
BstSCI CCNGG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 461, 688
BstV2I GAAGAC 2 cut(s) 154, 673
BstX2I RGATCY 2 cut(s) 1099, 1201
BstYI RGATCY 2 cut(s) 1099, 1201
BstZI CGGCCG 1 cut(s) 667
BsuRI GGCC 1 cut(s) 669
BtsCI GGATG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 870, 906, 1196
CaiI CAGNNNCTG 1 cut(s) 1021
CciI TCATGA 2 cut(s) 465, 993
Cfr10I RCCGGY 2 cut(s) 665, 787
Cfr13I GGNCC 1 cut(s) 497
CseI GACGC 2 cut(s) 74, 158
Csp6I GTAC 4 cut(s) 7, 76, 785, 853
CspAI ACCGGT 1 cut(s) 787
CviAII CATG 8 cut(s) 230, 466, 655, 848, 905, 920, 994, 1096
CviQI GTAC 4 cut(s) 7, 76, 785, 853
DdeI CTNAG 5 cut(s) 875, 890, 1022, 1076, 1190
DpnI GATC 5 cut(s) 888, 927, 1029, 1101, 1203
DpnII GATC 5 cut(s) 886, 925, 1027, 1099, 1201
EaeI YGGCCR 1 cut(s) 667
EagI CGGCCG 1 cut(s) 667
Eam1104I CTCTTC 6 cut(s) 91, 121, 138, 465, 631, 915
EarI CTCTTC 6 cut(s) 91, 121, 138, 465, 631, 915
Ecl136II GAGCTC 3 cut(s) 826, 973, 1040
EclXI CGGCCG 1 cut(s) 667
Eco24I GRGCYC 3 cut(s) 828, 975, 1042
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 497
Eco52I CGGCCG 1 cut(s) 667
Eco53kI GAGCTC 3 cut(s) 826, 973, 1040
Eco57I CTGAAG 1 cut(s) 1070
EcoICRI GAGCTC 3 cut(s) 826, 973, 1040
EcoRII CCWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 3 cut(s) 828, 975, 1042
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 8 cut(s) 233, 469, 658, 851, 908, 923, 997, 1099
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 533, 565
FatI CATG 8 cut(s) 229, 465, 654, 847, 904, 919, 993, 1095
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 2 cut(s) 450, 702
FokI GGATG 1 cut(s) 778
FriOI GRGCYC 3 cut(s) 828, 975, 1042
Fsp4HI GCNGC 2 cut(s) 450, 702
FspBI CTAG 4 cut(s) 174, 269, 968, 1085
GluI GCNGC 2 cut(s) 450, 702
GsaI CCCAGC 2 cut(s) 456, 492
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 669
HapII CCGG 4 cut(s) 31, 666, 788, 901
HgaI GACGC 2 cut(s) 74, 158
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 8 cut(s) 233, 469, 658, 851, 908, 923, 997, 1099
HindIII AAGCTT 3 cut(s) 554, 794, 1052
HpaII CCGG 4 cut(s) 31, 666, 788, 901
HphI GGTGA 5 cut(s) 20, 32, 802, 896, 1034
Hpy166II GTNNAC 2 cut(s) 855, 982
Hpy188I TCNGA 5 cut(s) 196, 208, 512, 609, 876
Hpy188III TCNNGA 8 cut(s) 317, 434, 466, 541, 914, 934, 994, 1103
Hpy8I GTNNAC 2 cut(s) 855, 982
Hpy99I CGWCG 2 cut(s) 68, 152
HpyAV CCTTC 1 cut(s) 1052
HpyCH4III ACNGT 2 cut(s) 192, 565
HpyCH4IV ACGT 2 cut(s) 9, 159
HpyCH4V TGCA 5 cut(s) 308, 730, 738, 847, 868
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 353, 398, 901
HpyF3I CTNAG 5 cut(s) 875, 890, 1022, 1076, 1190
HpySE526I ACGT 2 cut(s) 9, 159
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 8 cut(s) 233, 469, 658, 851, 908, 923, 997, 1099
KpnI GGTACC 1 cut(s) 788
Kzo9I GATC 5 cut(s) 886, 925, 1027, 1099, 1201
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 3 cut(s) 512, 831, 978
Lsp1109I GCAGC 2 cut(s) 461, 688
MaeI CTAG 4 cut(s) 174, 269, 968, 1085
MaeII ACGT 2 cut(s) 9, 159
MaeIII GTNAC 3 cut(s) 251, 523, 646
MalI GATC 5 cut(s) 888, 927, 1029, 1101, 1203
MboI GATC 5 cut(s) 886, 925, 1027, 1099, 1201
MfeI CAATTG 1 cut(s) 533
MflI RGATCY 2 cut(s) 1099, 1201
MhlI GDGCHC 3 cut(s) 828, 975, 1042
MlyI GAGTC 3 cut(s) 77, 516, 984
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 5 cut(s) 116, 141, 616, 930, 1102
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 4 cut(s) 413, 443, 552, 773
MslI CAYNNNNRTG 1 cut(s) 1078
MspA1I CMGCKG 2 cut(s) 452, 1048
MspI CCGG 4 cut(s) 31, 666, 788, 901
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 4 cut(s) 284, 353, 398, 901
NdeI CATATG 1 cut(s) 721
NdeII GATC 5 cut(s) 886, 925, 1027, 1099, 1201
NlaIII CATG 8 cut(s) 233, 469, 658, 851, 908, 923, 997, 1099
NlaIV GGNNCC 2 cut(s) 562, 786
NmuCI GTSAC 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 2 cut(s) 658, 908
PaeI GCATGC 1 cut(s) 908
PagI TCATGA 2 cut(s) 465, 993
PciI ACATGT 1 cut(s) 654
PciSI GCTCTTC 1 cut(s) 915
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 7 cut(s) 36, 57, 170, 201, 220, 265, 335
Pfl23II CGTACG 1 cut(s) 6
PfoI TCCNGGA 1 cut(s) 165
PinAI ACCGGT 1 cut(s) 787
PkrI GCNGC 2 cut(s) 451, 703
PleI GAGTC 3 cut(s) 77, 515, 984
PpsI GAGTC 3 cut(s) 77, 515, 984
PscI ACATGT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 3 cut(s) 828, 975, 1042
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 2 cut(s) 452, 488
PspGI CCWGG 1 cut(s) 165
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 562, 786
PspPI GGNCC 1 cut(s) 497
PstNI CAGNNNCTG 1 cut(s) 1021
PsuI RGATCY 2 cut(s) 1099, 1201
PvuII CAGCTG 2 cut(s) 452, 1048
RsaI GTAC 4 cut(s) 8, 77, 786, 854
RsaNI GTAC 4 cut(s) 7, 76, 785, 853
RseI CAYNNNNRTG 1 cut(s) 1078
SacI GAGCTC 3 cut(s) 828, 975, 1042
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 4 cut(s) 413, 443, 552, 773
SatI GCNGC 2 cut(s) 450, 702
Sau3AI GATC 5 cut(s) 886, 925, 1027, 1099, 1201
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 3 cut(s) 77, 516, 984
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 3 cut(s) 828, 975, 1042
SinI GGWCC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 1078
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
SphI GCATGC 1 cut(s) 908
SsiI CCGC 1 cut(s) 392
SspMI CTAG 4 cut(s) 174, 269, 968, 1085
SstI GAGCTC 3 cut(s) 828, 975, 1042
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 192, 565
TaiI ACGT 2 cut(s) 12, 162
TaqI TCGA 5 cut(s) 273, 291, 382, 577, 597
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 7 cut(s) 36, 57, 170, 201, 220, 265, 335
Tru1I TTAA 4 cut(s) 413, 443, 552, 773
Tru9I TTAA 4 cut(s) 413, 443, 552, 773
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 449, 701
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 4 cut(s) 390, 482, 936, 1010
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 497
XapI RAATTY 3 cut(s) 378, 691, 1002
XceI RCATGY 2 cut(s) 658, 908
XmiI GTMKAC 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 4 cut(s) 174, 269, 968, 1085
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.