AT2G20495

kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001830
Physical Location & Seq
Forward (+)
8832957 .. 8835263
2307 bp
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UTR
Exon/CDS
Intron
AT2G20495.4

Sequence Viewer

Length: 849 bp
ATGAAGAAAAGAACAAATCCGGAATCGTCCACCTCTGAAGCAAAGAAACGCCGGCGAGAGGAAGATGACGATAAAGTTGGTTGTAGTCACTCTGCAATTGAAGATGGAGCAGTATCTGAGGATCAATGTCTTCCTCTTGAGGATGATCTCACCTTTAGCGATACTTCTGTTGCCCTTCGAATGATGCGAGCTCAGTTTCCTCGTATTGACCAGGCTTCGATCCCACCCTTCATATTACAGTCGCAATTGTACAGTAGTGTCAATGATAGAACCCAAGTCGATAGAGAATTAGAGTGTTTACGAAGGGAGAAAGTTGTTCGTGTTTTCAAACTCAACACTGGACAAGATGATCATGCCATCATCTTTTTGGATGACTATCTTAACCAGGTAGATCGAATAGTGAAGAGGATGGAAGAGAAGAAACAGAGTGATCTTGAAATTTTCAAGTTGTTCAAAATGCATGTCCTTGATTCAAAACTTGAACCTAGTATCGGGCATCATGAGCTACTTTCGCTGTTGTCATTGGGAGGAAAGGTGAAAGATGCACACATCACTCTCTTAATAAACGCAGGCCTTCTCACACGCCAACTTATCGATCCAGACATGTACTGGTTTGCAATTCCGAGTACCGGTAAATTGTGGAAGGGTCTATCGCAGGGAAGGAACGAACTCCTCTCATTGATAAAGCGCAAGCGACATAAAGAGATGTTCTTGGCTGAGCTTGAAAAGAAACGGCTCCGGTTCTCTCCTCTGGATGTGAGATTTCACGTACGTGATCTCATTGGCTCCGGTCACCTGAAAACTGTTCAGACAACTTCTGGACTGATCGTTCGGATTTTAAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

282

Amino Acids

32.49

Weight (kDa)

8.85

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 93 - 301 1.3e-35 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 19
AclWI GGATC 3 cut(s) 129, 214, 590
AcsI RAATTY 1 cut(s) 438
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 4 cut(s) 251, 608, 628, 771
AfiI CCNNNNNNNGG 3 cut(s) 58, 491, 629
AflIII ACRYGT 1 cut(s) 603
AgeI ACCGGT 1 cut(s) 629
AgsI TTSAA 8 cut(s) 101, 328, 437, 445, 454, 474, 482, 725
AjnI CCWGG 2 cut(s) 210, 384
AleI CACNNNNGTG 1 cut(s) 771
AloI GAACNNNNNNTCC 2 cut(s) 813, 845
AluBI AGCT 3 cut(s) 191, 505, 721
AluI AGCT 3 cut(s) 191, 505, 721
Alw21I GWGCWC 1 cut(s) 193
AlwI GGATC 3 cut(s) 129, 214, 590
AlwNI CAGNNNCTG 1 cut(s) 116
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 1 cut(s) 571
ApoI RAATTY 1 cut(s) 438
ArsI GACNNNNNNTTYG 4 cut(s) 447, 479, 813, 845
AsiGI ACCGGT 1 cut(s) 629
AspLEI GCGC 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 142, 547, 785
AsuII TTCGAA 1 cut(s) 178
BaeI ACNNNNGTAYC 2 cut(s) 153, 186
BanII GRGCYC 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 193
BccI CCATC 3 cut(s) 98, 365, 403
BceAI ACGGC 1 cut(s) 749
BciT130I CCWGG 2 cut(s) 212, 386
BclI TGATCA 1 cut(s) 349
BfaI CTAG 1 cut(s) 486
BlpI GCTNAGC 1 cut(s) 717
Bme1390I CCNGG 2 cut(s) 212, 386
BmiI GGNNCC 2 cut(s) 737, 787
BmrFI CCNGG 2 cut(s) 212, 386
BmsI GCATC 3 cut(s) 174, 505, 532
BpiI GAAGAC 1 cut(s) 122
Bpu1102I GCTNAGC 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 594
BsaAI YACGTR 2 cut(s) 769, 773
BsaBI GATNNNNATC 1 cut(s) 375
BsaWI WCCGGW 4 cut(s) 19, 629, 738, 788
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 491, 629
Bse118I RCCGGY 2 cut(s) 51, 629
Bse1I ACTGG 2 cut(s) 343, 614
Bse8I GATNNNNATC 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 2 cut(s) 212, 386
BseCI ATCGAT 1 cut(s) 594
BseGI GGATG 4 cut(s) 148, 376, 414, 760
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 58, 491, 629
BseMII CTCAG 3 cut(s) 108, 206, 708
BseNI ACTGG 2 cut(s) 343, 614
BseRI GAGGAG 2 cut(s) 662, 738
BshFI GGCC 1 cut(s) 573
BshTI ACCGGT 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 594
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 5 cut(s) 20, 52, 630, 739, 789
BsiWI CGTACG 1 cut(s) 769
BslI CCNNNNNNNGG 3 cut(s) 58, 491, 629
BsnI GGCC 1 cut(s) 573
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 19
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
Bsp1720I GCTNAGC 1 cut(s) 717
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 3 cut(s) 109, 205, 709
BspDI ATCGAT 1 cut(s) 594
BspEI TCCGGA 1 cut(s) 19
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 2 cut(s) 737, 787
BspPI GGATC 3 cut(s) 129, 214, 590
BspT104I TTCGAA 1 cut(s) 178
BsrFI RCCGGY 2 cut(s) 51, 629
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 2 cut(s) 343, 614
BssAI RCCGGY 2 cut(s) 51, 629
BssMI GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
Bst2UI CCWGG 2 cut(s) 212, 386
Bst4CI ACNGT 3 cut(s) 240, 254, 805
Bst6I CTCTTC 2 cut(s) 398, 408
BstAUI TGTACA 1 cut(s) 249
BstBAI YACGTR 2 cut(s) 769, 773
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 4 cut(s) 53, 189, 571, 692
BstDEI CTNAG 3 cut(s) 117, 192, 717
BstEII GGTNACC 1 cut(s) 791
BstF5I GGATG 4 cut(s) 148, 376, 414, 760
BstHHI GCGC 1 cut(s) 690
BstKTI GATC 9 cut(s) 124, 148, 222, 352, 394, 433, 598, 778, 828
BstMBI GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
BstMWI GCNNNNNNNGC 2 cut(s) 502, 511
BstNI CCWGG 2 cut(s) 212, 386
BstNSI RCATGY 2 cut(s) 464, 607
BstPI GGTNACC 1 cut(s) 791
BstSCI CCNGG 2 cut(s) 210, 384
BstV2I GAAGAC 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 594
BsuRI GGCC 1 cut(s) 573
BsuTUI ATCGAT 1 cut(s) 594
BtsCI GGATG 4 cut(s) 148, 376, 414, 760
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 4 cut(s) 53, 189, 571, 692
CaiI CAGNNNCTG 1 cut(s) 116
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 690
Cfr10I RCCGGY 2 cut(s) 51, 629
ClaI ATCGAT 1 cut(s) 594
CsiI ACCWGGT 1 cut(s) 384
Csp6I GTAC 4 cut(s) 250, 607, 627, 770
CspAI ACCGGT 1 cut(s) 629
CviAII CATG 4 cut(s) 353, 461, 500, 604
CviJI RGCY 8 cut(s) 191, 215, 505, 573, 716, 721, 736, 786
CviKI_1 RGCY 8 cut(s) 191, 215, 505, 573, 716, 721, 736, 786
CviQI GTAC 4 cut(s) 250, 607, 627, 770
DdeI CTNAG 3 cut(s) 117, 192, 717
DpnI GATC 9 cut(s) 123, 147, 221, 351, 393, 432, 597, 777, 827
DpnII GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
DraI TTTAAA 1 cut(s) 840
Eam1104I CTCTTC 2 cut(s) 398, 408
EarI CTCTTC 2 cut(s) 398, 408
Ecl136II GAGCTC 1 cut(s) 191
Eco147I AGGCCT 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 791
EcoICRI GAGCTC 1 cut(s) 191
EcoO65I GGTNACC 1 cut(s) 791
EcoRII CCWGG 2 cut(s) 210, 384
EcoT22I ATGCAT 1 cut(s) 462
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 4 cut(s) 356, 464, 503, 607
FaiI YATR 6 cut(s) 233, 354, 462, 501, 605, 699
FatI CATG 4 cut(s) 352, 460, 499, 603
FbaI TGATCA 1 cut(s) 349
FokI GGATG 4 cut(s) 155, 383, 421, 767
FriOI GRGCYC 1 cut(s) 193
FspBI CTAG 1 cut(s) 486
GlaI GCGC 1 cut(s) 689
HaeIII GGCC 1 cut(s) 573
HapII CCGG 5 cut(s) 20, 52, 630, 739, 789
HhaI GCGC 1 cut(s) 690
Hin1II CATG 4 cut(s) 356, 464, 503, 607
Hin6I GCGC 1 cut(s) 688
HinP1I GCGC 1 cut(s) 688
HinfI GANTC 2 cut(s) 23, 470
HpaII CCGG 5 cut(s) 20, 52, 630, 739, 789
HphI GGTGA 3 cut(s) 142, 547, 785
Hpy166II GTNNAC 2 cut(s) 30, 299
Hpy188I TCNGA 5 cut(s) 37, 118, 624, 810, 834
Hpy188III TCNNGA 7 cut(s) 20, 137, 434, 500, 599, 752, 819
Hpy8I GTNNAC 2 cut(s) 30, 299
HpyAV CCTTC 6 cut(s) 185, 238, 297, 584, 637, 654
HpyCH4III ACNGT 3 cut(s) 240, 254, 805
HpyCH4IV ACGT 2 cut(s) 768, 772
HpyCH4V TGCA 4 cut(s) 95, 460, 545, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 511
HpyF3I CTNAG 3 cut(s) 117, 192, 717
HpySE526I ACGT 2 cut(s) 768, 772
Hsp92II CATG 4 cut(s) 356, 464, 503, 607
HspAI GCGC 1 cut(s) 688
Kpn2I TCCGGA 1 cut(s) 19
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
LmnI GCTCC 3 cut(s) 107, 741, 791
LweI GCATC 3 cut(s) 174, 505, 532
MabI ACCWGGT 1 cut(s) 384
MaeI CTAG 1 cut(s) 486
MaeII ACGT 2 cut(s) 768, 772
MaeIII GTNAC 2 cut(s) 86, 791
MalI GATC 9 cut(s) 123, 147, 221, 351, 393, 432, 597, 777, 827
MboI GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
MboII GAAGA 7 cut(s) 16, 74, 113, 122, 415, 425, 430
MfeI CAATTG 2 cut(s) 96, 245
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 6 cut(s) 96, 245, 287, 438, 618, 635
Mph1103I ATGCAT 1 cut(s) 462
MreI CGCCGGCG 1 cut(s) 51
MroI TCCGGA 1 cut(s) 19
MroNI GCCGGC 1 cut(s) 51
MseI TTAA 3 cut(s) 381, 560, 839
MslI CAYNNNNRTG 1 cut(s) 771
MspI CCGG 5 cut(s) 20, 52, 630, 739, 789
MspR9I CCNGG 2 cut(s) 212, 386
MunI CAATTG 2 cut(s) 96, 245
MvaI CCWGG 2 cut(s) 212, 386
MwoI GCNNNNNNNGC 2 cut(s) 502, 511
NaeI GCCGGC 1 cut(s) 53
NdeII GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 4 cut(s) 356, 464, 503, 607
NlaIV GGNNCC 2 cut(s) 737, 787
NmuCI GTSAC 2 cut(s) 86, 791
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 2 cut(s) 464, 607
NspV TTCGAA 1 cut(s) 178
OliI CACNNNNGTG 1 cut(s) 771
PagI TCATGA 1 cut(s) 499
PceI AGGCCT 1 cut(s) 573
PciI ACATGT 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 184
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 2 cut(s) 23, 470
Pfl23II CGTACG 1 cut(s) 769
PinAI ACCGGT 1 cut(s) 629
Ppu21I YACGTR 2 cut(s) 769, 773
PscI ACATGT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 193
Psp6I CCWGG 2 cut(s) 210, 384
PspEI GGTNACC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 210, 384
PspLI CGTACG 1 cut(s) 769
PspN4I GGNNCC 2 cut(s) 737, 787
PstNI CAGNNNCTG 1 cut(s) 116
RsaI GTAC 4 cut(s) 251, 608, 628, 771
RsaNI GTAC 4 cut(s) 250, 607, 627, 770
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 193
SaqAI TTAA 3 cut(s) 381, 560, 839
Sau3AI GATC 9 cut(s) 121, 145, 219, 349, 391, 430, 595, 775, 825
ScrFI CCNGG 2 cut(s) 212, 386
SduI GDGCHC 1 cut(s) 193
SexAI ACCWGGT 1 cut(s) 384
SfaNI GCATC 3 cut(s) 174, 505, 532
SfuI TTCGAA 1 cut(s) 178
SgrAI CRCCGGYG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 6 cut(s) 96, 245, 287, 438, 618, 635
SseBI AGGCCT 1 cut(s) 573
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 193
StuI AGGCCT 1 cut(s) 573
StyD4I CCNGG 2 cut(s) 210, 384
TaaI ACNGT 3 cut(s) 240, 254, 805
TaiI ACGT 2 cut(s) 771, 775
TaqI TCGA 5 cut(s) 178, 218, 279, 394, 594
TasI AATT 6 cut(s) 96, 245, 287, 438, 618, 635
TatI WGTACW 2 cut(s) 249, 606
TfiI GAWTC 2 cut(s) 23, 470
Tru1I TTAA 3 cut(s) 381, 560, 839
Tru9I TTAA 3 cut(s) 381, 560, 839
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 2 cut(s) 86, 791
Tsp45I GTSAC 2 cut(s) 86, 791
TspDTI ATGAA 2 cut(s) 17, 220
TspRI CASTG 1 cut(s) 343
XapI RAATTY 1 cut(s) 438
XceI RCATGY 2 cut(s) 464, 607
XcmI CCANNNNNNNNNTGG 2 cut(s) 364, 606
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.