FvH4_2g21270

Serine-threonine protein kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001830
Physical Location & Seq
Forward (+)
17666462 .. 17669982
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21270.t9

Sequence Viewer

Length: 573 bp
ATGGCCAGAAATATTTCCGATTCTTTGAAAGGTAAAAAGCGCGCGCGGGAATCCGACGCCGAACCCGAACCCGAACCGGAATCCGGCGATATCGATCAGATTCTGTCATTAGAGGAAAATCTTACCTTTAGTGACACATTGGTGGCGCTTCGCATAATGCGAGAGCAGTTTCCCCAAATTGACAAGCCAATTCAGCCTTTCATTTTGCGGTCACAGCTGTATAGCAGTGTAGAGGACAGGACACAAGTGGACCGGGACCTGGAGACTCTAAGAAGAGAGAAAGTGTTGCGCATCTTCAAATTAAATACAGGGCAGGATGATCATGCAATAATTTTTTTCGATGACTACCTATGCCAGGTAGACCGAGTTGTGAAAAGATTGGAAGGAGAAAAGGAAAGAGATATTCTTGAAGTTTTCAAGTGGTTCAGATCACATGTTATTGATTTCAAGCTTGAACCTAGTATTGAACACCAAGAACTTTGTGCACTATTATCTCTTGGAGGAAAGGTCAAAGATGAACACATCTCCCTTTTAATGAATTCTGGCCTTCTTGTAAGTAAACTTGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

191

Amino Acids

22.12

Weight (kDa)

5.14

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 40 - 210 3e-16 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 290
AccI GTMKAC 1 cut(s) 360
AccII CGCG 3 cut(s) 42, 44, 46
AciI CCGC 2 cut(s) 46, 208
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 538
AcyI GRCGYC 1 cut(s) 57
AfiI CCNNNNNNNGG 3 cut(s) 83, 259, 355
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 8 cut(s) 28, 298, 410, 418, 448, 455, 467, 566
AjnI CCWGG 2 cut(s) 258, 354
AleI CACNNNNGTG 1 cut(s) 140
AluBI AGCT 2 cut(s) 217, 451
AluI AGCT 2 cut(s) 217, 451
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 1 cut(s) 257
Alw44I GTGCAC 1 cut(s) 483
AlwNI CAGNNNCTG 1 cut(s) 103
AoxI GGCC 2 cut(s) 3, 544
ApaLI GTGCAC 1 cut(s) 483
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 5 cut(s) 42, 44, 46, 148, 291
AspS9I GGNCC 2 cut(s) 250, 256
AsuC2I CCSGG 1 cut(s) 254
AvaII GGWCC 2 cut(s) 250, 256
BaeGI GKGCMC 1 cut(s) 487
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 487
BciT130I CCWGG 2 cut(s) 260, 356
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 149
Bme1390I CCNGG 3 cut(s) 254, 260, 356
Bme18I GGWCC 2 cut(s) 250, 256
BmgT120I GGNCC 2 cut(s) 250, 256
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 3 cut(s) 254, 260, 356
BmsI GCATC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 93
BsaBI GATNNNNATC 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 259, 355
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 2 cut(s) 260, 356
BseCI ATCGAT 1 cut(s) 93
BseGI GGATG 1 cut(s) 322
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 83, 259, 355
BsePI GCGCGC 2 cut(s) 40, 42
BseSI GKGCMC 1 cut(s) 487
Bsh1236I CGCG 3 cut(s) 42, 44, 46
BshFI GGCC 2 cut(s) 5, 546
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 3 cut(s) 77, 84, 253
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 83, 259, 355
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 2 cut(s) 5, 546
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 3 cut(s) 94, 319, 428
BspACI CCGC 2 cut(s) 46, 208
BspANI GGCC 2 cut(s) 5, 546
BspDI ATCGAT 1 cut(s) 93
BspFNI CGCG 3 cut(s) 42, 44, 46
BspLI GGNNCC 1 cut(s) 257
BssHII GCGCGC 2 cut(s) 40, 42
BssMI GATC 3 cut(s) 94, 319, 428
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 260, 356
Bst6I CTCTTC 1 cut(s) 268
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 42, 44
BstDEI CTNAG 1 cut(s) 269
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 3 cut(s) 42, 44, 46
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 5 cut(s) 42, 44, 46, 148, 291
BstKTI GATC 3 cut(s) 97, 322, 431
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 3 cut(s) 94, 319, 428
BstMWI GCNNNNNNNGC 2 cut(s) 193, 214
BstNI CCWGG 2 cut(s) 260, 356
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 252, 258, 354
BstSLI GKGCMC 1 cut(s) 487
BstUI CGCG 3 cut(s) 42, 44, 46
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 2 cut(s) 5, 546
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 42, 44
CaiI CAGNNNCTG 1 cut(s) 103
CfoI GCGC 5 cut(s) 42, 44, 46, 148, 291
Cfr13I GGNCC 2 cut(s) 250, 256
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 65
CviAII CATG 2 cut(s) 323, 434
CviJI RGCY 6 cut(s) 5, 187, 196, 217, 451, 546
CviKI_1 RGCY 6 cut(s) 5, 187, 196, 217, 451, 546
DdeI CTNAG 1 cut(s) 269
DpnI GATC 3 cut(s) 96, 321, 430
DpnII GATC 3 cut(s) 94, 319, 428
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 250, 256
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 2 cut(s) 258, 354
EcoRV GATATC 1 cut(s) 91
FaeI CATG 2 cut(s) 326, 437
FaiI YATR 5 cut(s) 155, 222, 324, 352, 435
FaqI GGGAC 1 cut(s) 269
FatI CATG 2 cut(s) 322, 433
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 360
FokI GGATG 1 cut(s) 329
FspBI CTAG 1 cut(s) 459
FspI TGCGCA 1 cut(s) 290
GlaI GCGC 5 cut(s) 41, 43, 45, 147, 290
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 5, 546
HapII CCGG 3 cut(s) 77, 84, 253
HgaI GACGC 1 cut(s) 65
HhaI GCGC 5 cut(s) 42, 44, 46, 148, 291
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 326, 437
Hin6I GCGC 5 cut(s) 40, 42, 44, 146, 289
HinP1I GCGC 5 cut(s) 40, 42, 44, 146, 289
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 5 cut(s) 20, 50, 80, 100, 265
HpaII CCGG 3 cut(s) 77, 84, 253
Hpy166II GTNNAC 4 cut(s) 250, 361, 485, 560
Hpy188I TCNGA 4 cut(s) 19, 55, 99, 428
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 4 cut(s) 250, 361, 485, 560
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 377, 557
HpyCH4V TGCA 2 cut(s) 326, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 214
HpyF3I CTNAG 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 326, 437
HspAI GCGC 5 cut(s) 40, 42, 44, 146, 289
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 94, 319, 428
LweI GCATC 1 cut(s) 300
MaeI CTAG 1 cut(s) 459
MaeIII GTNAC 2 cut(s) 131, 210
MalI GATC 3 cut(s) 96, 321, 430
MauBI CGCGCGCG 1 cut(s) 42
MboI GATC 3 cut(s) 94, 319, 428
MboII GAAGA 2 cut(s) 285, 286
MhlI GDGCHC 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 177, 189, 299, 330, 538
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 3 cut(s) 106, 226, 494
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 13
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 302, 533
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 3 cut(s) 77, 84, 253
MspR9I CCNGG 3 cut(s) 254, 260, 356
MvaI CCWGG 2 cut(s) 260, 356
MvnI CGCG 3 cut(s) 42, 44, 46
MwoI GCNNNNNNNGC 2 cut(s) 193, 214
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 94, 319, 428
NlaIII CATG 2 cut(s) 326, 437
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 2 cut(s) 131, 210
NsbI TGCGCA 1 cut(s) 290
NspI RCATGY 1 cut(s) 437
OliI CACNNNNGTG 1 cut(s) 140
PauI GCGCGC 2 cut(s) 40, 42
PciI ACATGT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 157
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 4 cut(s) 20, 50, 80, 100
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 258, 354
PspGI CCWGG 2 cut(s) 258, 354
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 2 cut(s) 250, 256
PspPPI RGGWCCY 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 103
PteI GCGCGC 2 cut(s) 40, 42
PvuII CAGCTG 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 302, 533
Sau3AI GATC 3 cut(s) 94, 319, 428
Sau96I GGNCC 2 cut(s) 250, 256
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 3 cut(s) 254, 260, 356
SduI GDGCHC 1 cut(s) 487
SetI ASST 9 cut(s) 34, 128, 219, 261, 351, 360, 453, 460, 510
SfaNI GCATC 1 cut(s) 300
SinI GGWCC 2 cut(s) 250, 256
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 5 cut(s) 177, 189, 299, 330, 538
SsiI CCGC 2 cut(s) 46, 208
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 459
StyD4I CCNGG 3 cut(s) 252, 258, 354
TaqI TCGA 2 cut(s) 93, 339
TaqII GACCGA 1 cut(s) 378
TasI AATT 5 cut(s) 177, 189, 299, 330, 538
TfiI GAWTC 4 cut(s) 20, 50, 80, 100
Tru1I TTAA 2 cut(s) 302, 533
Tru9I TTAA 2 cut(s) 302, 533
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 2 cut(s) 131, 210
Tsp45I GTSAC 2 cut(s) 131, 210
TspDTI ATGAA 3 cut(s) 190, 531, 551
TspRI CASTG 1 cut(s) 232
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 2 cut(s) 250, 256
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 1 cut(s) 538
XceI RCATGY 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.