Rh6AG302100

Serine-threonine protein kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001830
Physical Location & Seq
Forward (+)
50059526 .. 50063412
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG302100.1

Sequence Viewer

Length: 993 bp
ATGGTCCAAGACTGTAAATTTACGACTTTACCCCGACCGACATGGAACCCCATCAAGCTGCGAACTTTGCGCAACCCCGCCATCACAATCGGACAGCCAGAAAAGAAAAGGGTGGGTTCGAATTTAGGGCTTCTCCGTTTTGCCCTAAATTCCCAATTCGAATTTCAATCGTGCCCACAAAAGATGGCCGGAAATATTTCAGAATCTTTGAAAGGTAAAAAGCGCGCGCGAGAATCCCACGCCGAACCCGAAACCGCCGATATCGATCATATTCTATCGTTAGAGGAAAATCTTACGTTTAGTGATACATTGGTGGCTCTTCGCATAATGCGAGAACAGTTTCCGCAAATTGACAAGCCAATCCAGCCTTTCATTTTGCGGTCACAGTTGTATAGCAGTGTAGAGGACAGGACACAAGTGGACCGGGACCTGGAGACTCTAAGAAGGGAGAAAGTGTTGCGCATTTTTAAATTAAATACGGGGCAAGATGATCATGCCATAATGTTTTTGGATGACTACCTATGCCAGGTAGACCAAGTTGTGAAAAGATTGGAAGGAGAAAAGGGAAGAGATGTTCTTGAAGTTTTTAACTGGTTCAGATCACACGTTATTGATTTCAAGCTTGAACCTAGTATTGAACACCAAGAACTTTGTACATTATTATCACTTGGAGGAAAGGTTAAAGATGAACACATCTCACTTTTAATGAATTCTGGCCTTCTTACTCGGCAACTTATTGACCCAAACATTTACTGGTTCGCAATTCCAAATATCGGTTCAGTACTCAAAGGCCTCTCTCAGGGAAGAAAGGAAGTTCTTTCCCTTTTAAACCGCAGGAGATACAAAGAAATGATGTTGGCACCCCTAGAGAAGAAGCGTCTTAGATTCTCTTCACTTGATATGAGATTTCATCTTCGTGATCTGATAGGCTCAGGTCATCTCAAAACCGTCCAAATACCAACAGGTATAGTTGTACGAGTATCAAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

330

Amino Acids

38.13

Weight (kDa)

9.37

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 97 - 327 1.3e-30 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 71, 461
AccB1I GGYRCC 1 cut(s) 859
AccI GTMKAC 1 cut(s) 531
AccII CGCG 3 cut(s) 225, 227, 229
AciI CCGC 5 cut(s) 78, 255, 344, 379, 832
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 5 cut(s) 17, 121, 148, 161, 709
AfaI GTAC 3 cut(s) 655, 783, 975
AfiI CCNNNNNNNGG 4 cut(s) 430, 526, 773, 799
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 6 cut(s) 167, 211, 581, 619, 626, 638
AjnI CCWGG 2 cut(s) 429, 525
AjuI GAANNNNNNNTTGG 4 cut(s) 147, 179, 760, 792
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
AluBI AGCT 2 cut(s) 58, 622
AluI AGCT 2 cut(s) 58, 622
Alw26I GTCTC 1 cut(s) 428
AoxI GGCC 3 cut(s) 186, 715, 790
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 5 cut(s) 17, 121, 148, 161, 709
Asp700I GAANNNNTTC 2 cut(s) 196, 339
AspLEI GCGC 5 cut(s) 72, 225, 227, 229, 462
AspS9I GGNCC 3 cut(s) 4, 421, 427
AsuC2I CCSGG 1 cut(s) 425
AsuII TTCGAA 2 cut(s) 119, 159
AvaII GGWCC 3 cut(s) 4, 421, 427
BaeGI GKGCMC 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BanI GGYRCC 1 cut(s) 859
BbvI GCAGC 1 cut(s) 45
BccI CCATC 3 cut(s) 59, 89, 178
BciT130I CCWGG 2 cut(s) 431, 527
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 2 cut(s) 630, 866
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 425, 431, 527
Bme18I GGWCC 3 cut(s) 4, 421, 427
BmgT120I GGNCC 3 cut(s) 4, 421, 427
BmiI GGNNCC 3 cut(s) 47, 428, 861
BmrFI CCNGG 3 cut(s) 425, 431, 527
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 931
Bpu14I TTCGAA 2 cut(s) 119, 159
BpuMI CCSGG 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 264
BsaXI ACNNNNNCTCC 4 cut(s) 440, 470, 663, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 430, 526, 773, 799
Bse1I ACTGG 2 cut(s) 596, 758
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 2 cut(s) 431, 527
BseCI ATCGAT 1 cut(s) 264
BseGI GGATG 1 cut(s) 517
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 4 cut(s) 430, 526, 773, 799
BseMII CTCAG 2 cut(s) 812, 945
BseNI ACTGG 2 cut(s) 596, 758
BsePI GCGCGC 2 cut(s) 223, 225
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 1 cut(s) 45
Bsh1236I CGCG 3 cut(s) 225, 227, 229
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 188, 717, 792
BshNI GGYRCC 1 cut(s) 859
BshVI ATCGAT 1 cut(s) 264
BsiEI CGRYCG 1 cut(s) 38
BsiSI CCGG 2 cut(s) 189, 424
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 4 cut(s) 430, 526, 773, 799
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 3 cut(s) 188, 717, 792
Bsp119I TTCGAA 2 cut(s) 119, 159
Bsp1286I GDGCHC 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 653
Bsp143I GATC 4 cut(s) 265, 490, 599, 919
BspACI CCGC 5 cut(s) 78, 255, 344, 379, 832
BspANI GGCC 3 cut(s) 188, 717, 792
BspCNI CTCAG 2 cut(s) 811, 944
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 3 cut(s) 225, 227, 229
BspLI GGNNCC 3 cut(s) 47, 428, 861
BspQI GCTCTTC 1 cut(s) 324
BspT104I TTCGAA 2 cut(s) 119, 159
BspT107I GGYRCC 1 cut(s) 859
BsrGI TGTACA 1 cut(s) 653
BsrI ACTGG 2 cut(s) 596, 758
BssHII GCGCGC 2 cut(s) 223, 225
BssMI GATC 4 cut(s) 265, 490, 599, 919
Bst2UI CCWGG 2 cut(s) 431, 527
Bst4CI ACNGT 4 cut(s) 14, 339, 387, 949
Bst6I CTCTTC 3 cut(s) 324, 562, 895
BstAUI TGTACA 1 cut(s) 653
BstBI TTCGAA 2 cut(s) 119, 159
BstC8I GCNNGC 2 cut(s) 225, 227
BstDEI CTNAG 4 cut(s) 440, 798, 881, 931
BstENI CCTNNNNNAGG 2 cut(s) 524, 797
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 3 cut(s) 225, 227, 229
BstHHI GCGC 5 cut(s) 72, 225, 227, 229, 462
BstKTI GATC 4 cut(s) 268, 493, 602, 922
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 4 cut(s) 265, 490, 599, 919
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 67, 364
BstNI CCWGG 2 cut(s) 431, 527
BstSCI CCNGG 3 cut(s) 423, 429, 525
BstSLI GKGCMC 1 cut(s) 176
BstUI CGCG 3 cut(s) 225, 227, 229
BstV1I GCAGC 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 3 cut(s) 188, 717, 792
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 225, 227
CfoI GCGC 5 cut(s) 72, 225, 227, 229, 462
Cfr13I GGNCC 3 cut(s) 4, 421, 427
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 866
Csp6I GTAC 3 cut(s) 654, 782, 974
CviAII CATG 2 cut(s) 42, 494
CviQI GTAC 3 cut(s) 654, 782, 974
DdeI CTNAG 4 cut(s) 440, 798, 881, 931
DpnI GATC 4 cut(s) 267, 492, 601, 921
DpnII GATC 4 cut(s) 265, 490, 599, 919
DraI TTTAAA 2 cut(s) 469, 828
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 324, 562, 895
EarI CTCTTC 3 cut(s) 324, 562, 895
Eco147I AGGCCT 1 cut(s) 792
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 3 cut(s) 4, 421, 427
EcoNI CCTNNNNNAGG 2 cut(s) 524, 797
EcoO109I RGGNCCY 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 709
EcoRII CCWGG 2 cut(s) 429, 525
EcoRV GATATC 1 cut(s) 262
FaeI CATG 2 cut(s) 45, 497
FaiI YATR 9 cut(s) 43, 270, 326, 393, 495, 500, 523, 902, 968
FaqI GGGAC 1 cut(s) 440
FatI CATG 2 cut(s) 41, 493
FauI CCCGC 1 cut(s) 85
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 2 cut(s) 630, 866
FspI TGCGCA 2 cut(s) 71, 461
GlaI GCGC 5 cut(s) 71, 224, 226, 228, 461
GluI GCNGC 1 cut(s) 59
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 3 cut(s) 188, 717, 792
HapII CCGG 2 cut(s) 189, 424
HgaI GACGC 1 cut(s) 866
HhaI GCGC 5 cut(s) 72, 225, 227, 229, 462
Hin1II CATG 2 cut(s) 45, 497
Hin6I GCGC 5 cut(s) 70, 223, 225, 227, 460
HinP1I GCGC 5 cut(s) 70, 223, 225, 227, 460
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 4 cut(s) 203, 233, 436, 885
HpaII CCGG 2 cut(s) 189, 424
Hpy166II GTNNAC 2 cut(s) 421, 532
Hpy188I TCNGA 4 cut(s) 92, 202, 599, 924
Hpy188III TCNNGA 2 cut(s) 578, 917
Hpy8I GTNNAC 2 cut(s) 421, 532
HpyAV CCTTC 3 cut(s) 438, 548, 728
HpyCH4III ACNGT 4 cut(s) 14, 339, 387, 949
HpyCH4IV ACGT 2 cut(s) 296, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 364
HpyF3I CTNAG 4 cut(s) 440, 798, 881, 931
HpySE526I ACGT 2 cut(s) 296, 606
Hsp92II CATG 2 cut(s) 45, 497
HspAI GCGC 5 cut(s) 70, 223, 225, 227, 460
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 4 cut(s) 265, 490, 599, 919
LguI GCTCTTC 1 cut(s) 324
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 2 cut(s) 630, 866
MaeII ACGT 2 cut(s) 296, 606
MaeIII GTNAC 1 cut(s) 381
MalI GATC 4 cut(s) 267, 492, 601, 921
MauBI CGCGCGCG 1 cut(s) 225
MboI GATC 4 cut(s) 265, 490, 599, 919
MboII GAAGA 6 cut(s) 311, 579, 816, 882, 883, 905
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 9 cut(s) 17, 121, 148, 155, 161, 348, 470, 709, 762
MlyI GAGTC 1 cut(s) 430
MnlI CCTC 4 cut(s) 277, 397, 665, 803
MroXI GAANNNNTTC 2 cut(s) 196, 339
MseI TTAA 6 cut(s) 468, 473, 588, 681, 704, 827
MslI CAYNNNNRTG 1 cut(s) 915
MspI CCGG 2 cut(s) 189, 424
MspR9I CCNGG 3 cut(s) 425, 431, 527
MvaI CCWGG 2 cut(s) 431, 527
MvnI CGCG 3 cut(s) 225, 227, 229
MwoI GCNNNNNNNGC 2 cut(s) 67, 364
NciI CCSGG 1 cut(s) 425
NdeII GATC 4 cut(s) 265, 490, 599, 919
NlaIII CATG 2 cut(s) 45, 497
NlaIV GGNNCC 3 cut(s) 47, 428, 861
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 381
NsbI TGCGCA 2 cut(s) 71, 461
NspV TTCGAA 2 cut(s) 119, 159
PauI GCGCGC 2 cut(s) 223, 225
PceI AGGCCT 1 cut(s) 792
PciSI GCTCTTC 1 cut(s) 324
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 328
PdmI GAANNNNTTC 2 cut(s) 196, 339
PfeI GAWTC 3 cut(s) 203, 233, 885
PkrI GCNGC 1 cut(s) 60
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PpuMI RGGWCCY 1 cut(s) 427
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 429, 525
PspGI CCWGG 2 cut(s) 429, 525
PspN4I GGNNCC 3 cut(s) 47, 428, 861
PspPI GGNCC 3 cut(s) 4, 421, 427
PspPPI RGGWCCY 1 cut(s) 427
PteI GCGCGC 2 cut(s) 223, 225
RsaI GTAC 3 cut(s) 655, 783, 975
RsaNI GTAC 3 cut(s) 654, 782, 974
RseI CAYNNNNRTG 1 cut(s) 915
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 6 cut(s) 468, 473, 588, 681, 704, 827
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 4 cut(s) 265, 490, 599, 919
Sau96I GGNCC 3 cut(s) 4, 421, 427
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 3 cut(s) 425, 431, 527
SduI GDGCHC 1 cut(s) 176
SfuI TTCGAA 2 cut(s) 119, 159
SinI GGWCC 3 cut(s) 4, 421, 427
SmiMI CAYNNNNRTG 1 cut(s) 915
Sse9I AATT 9 cut(s) 17, 121, 148, 155, 161, 348, 470, 709, 762
SseBI AGGCCT 1 cut(s) 792
SsiI CCGC 5 cut(s) 78, 255, 344, 379, 832
SspI AATATT 1 cut(s) 196
SspMI CTAG 2 cut(s) 630, 866
StuI AGGCCT 1 cut(s) 792
StyD4I CCNGG 3 cut(s) 423, 429, 525
TaaI ACNGT 4 cut(s) 14, 339, 387, 949
TaiI ACGT 2 cut(s) 299, 609
TaqI TCGA 3 cut(s) 119, 159, 264
TaqII GACCGA 1 cut(s) 52
TasI AATT 9 cut(s) 17, 121, 148, 155, 161, 348, 470, 709, 762
TatI WGTACW 2 cut(s) 653, 781
TfiI GAWTC 3 cut(s) 203, 233, 885
Tru1I TTAA 6 cut(s) 468, 473, 588, 681, 704, 827
Tru9I TTAA 6 cut(s) 468, 473, 588, 681, 704, 827
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 4 cut(s) 361, 702, 722, 899
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 3 cut(s) 4, 421, 427
XagI CCTNNNNNAGG 2 cut(s) 524, 797
XapI RAATTY 5 cut(s) 17, 121, 148, 161, 709
XcmI CCANNNNNNNNNTGG 2 cut(s) 505, 750
XmiI GTMKAC 1 cut(s) 531
XmnI GAANNNNTTC 2 cut(s) 196, 339
XspI CTAG 2 cut(s) 630, 866
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.