Rroxscaffold_7G00180720

Serine-threonine protein kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001830
Physical Location & Seq
Reverse (-)
20056616 .. 20060736
4121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180720.1

Sequence Viewer

Length: 810 bp
ATGGCCGGAAATATTTCAGAATCTTTGAAAGGTAAAAAGCGCGCGCGAGAATCCGACGCCGAACCCGAAACCGCCGATATCGATCATATTCTATCGTTAGAGGAAAATCTTACGTTTAGTGATACATTGGTGGCTCTTCGCATAATGCGAGAACAGTTTCCGCAAATTGACAAGCCAATTCAGCCTTTCATTTTGCGGTCACAGTTGTATAGCAGTGTAGAGGACAGGACACAAGTGGACCGGGACCTGGAGACTCTAAGAAGGGAGAAAGTGTTGCGCATTTTTAAATTAAATACAGGGCAAGATGATCATGCCGTAATGTTTTTGGATGACTACCTATGCCAGGTAGACCGAGTTGTGAAAAGATTGGAAGGAGAAAAGGGAAGAGATGTTCTTGAAGTTTTTAACTGGTTCAGATCACATGTTATTGATTTCAAGCTTGAACCTAGTATTGAACACCAAGAACTTTGTACATTATTATCACTTGGAGGAAAGGTTAAAGATGAACACATCTCACTTTTAATGAATTCTGGCCTACTTACTCGGCAACTTATTGACCCAAACATTTACTGGTTTGCAATTCCAAATATCGGTTCAGTACTCAAAGGCCTCTCTCAGGGAAGAAAGGAAGTTCTTTCCCTTTTAAACCGCAGGAGATACAAAGAAATGATGTTGGCACCCCTAGAGAAGAAGCGTCTTAGATTCTCTTCACTTGATATGAGATTTCATCTTCGTGATCTGATAGGCTCAGGTCATCTCAAAACTGTCCAAATACCAACAGGCATAGTTGTACGAGTATCAAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

269

Amino Acids

31.12

Weight (kDa)

8.45

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 36 - 266 1.3e-30 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 348
AccII CGCG 3 cut(s) 42, 44, 46
AciI CCGC 4 cut(s) 72, 161, 196, 649
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 3 cut(s) 472, 600, 792
AfiI CCNNNNNNNGG 4 cut(s) 247, 343, 590, 616
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 5 cut(s) 28, 398, 436, 443, 455
AjnI CCWGG 2 cut(s) 246, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 577, 609
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 1 cut(s) 439
AluI AGCT 1 cut(s) 439
Alw26I GTCTC 1 cut(s) 245
AoxI GGCC 3 cut(s) 3, 532, 607
ApoI RAATTY 1 cut(s) 526
Asp700I GAANNNNTTC 2 cut(s) 13, 156
AspLEI GCGC 4 cut(s) 42, 44, 46, 279
AspS9I GGNCC 2 cut(s) 238, 244
AsuC2I CCSGG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 238, 244
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BanI GGYRCC 1 cut(s) 676
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 2 cut(s) 248, 344
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 2 cut(s) 447, 683
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 3 cut(s) 242, 248, 344
Bme18I GGWCC 2 cut(s) 238, 244
BmgT120I GGNCC 2 cut(s) 238, 244
BmiI GGNNCC 2 cut(s) 245, 678
BmrFI CCNGG 3 cut(s) 242, 248, 344
BpmI CTGGAG 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 748
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 57
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 480, 510
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 343, 590, 616
Bse1I ACTGG 2 cut(s) 413, 575
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 248, 344
BseCI ATCGAT 1 cut(s) 81
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 4 cut(s) 247, 343, 590, 616
BseMII CTCAG 2 cut(s) 629, 762
BseNI ACTGG 2 cut(s) 413, 575
BsePI GCGCGC 2 cut(s) 40, 42
Bsh1236I CGCG 3 cut(s) 42, 44, 46
BshFI GGCC 3 cut(s) 5, 534, 609
BshNI GGYRCC 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 81
BsiSI CCGG 2 cut(s) 6, 241
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 4 cut(s) 247, 343, 590, 616
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 3 cut(s) 5, 534, 609
Bsp1407I TGTACA 1 cut(s) 470
Bsp143I GATC 4 cut(s) 82, 307, 416, 736
BspACI CCGC 4 cut(s) 72, 161, 196, 649
BspANI GGCC 3 cut(s) 5, 534, 609
BspCNI CTCAG 2 cut(s) 628, 761
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 3 cut(s) 42, 44, 46
BspLI GGNNCC 2 cut(s) 245, 678
BspQI GCTCTTC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 676
BsrGI TGTACA 1 cut(s) 470
BsrI ACTGG 2 cut(s) 413, 575
BssHII GCGCGC 2 cut(s) 40, 42
BssMI GATC 4 cut(s) 82, 307, 416, 736
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 248, 344
Bst4CI ACNGT 3 cut(s) 156, 204, 766
Bst6I CTCTTC 3 cut(s) 141, 379, 712
BstACI GRCGYC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 42, 44
BstDEI CTNAG 4 cut(s) 257, 615, 698, 748
BstENI CCTNNNNNAGG 2 cut(s) 341, 614
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 3 cut(s) 42, 44, 46
BstHHI GCGC 4 cut(s) 42, 44, 46, 279
BstKTI GATC 4 cut(s) 85, 310, 419, 739
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 4 cut(s) 82, 307, 416, 736
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNI CCWGG 2 cut(s) 248, 344
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 3 cut(s) 240, 246, 342
BstUI CGCG 3 cut(s) 42, 44, 46
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 3 cut(s) 5, 534, 609
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 2 cut(s) 42, 44
CfoI GCGC 4 cut(s) 42, 44, 46, 279
Cfr13I GGNCC 2 cut(s) 238, 244
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 2 cut(s) 65, 683
Csp6I GTAC 3 cut(s) 471, 599, 791
CviAII CATG 2 cut(s) 311, 422
CviJI RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 609, 747
CviKI_1 RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 609, 747
CviQI GTAC 3 cut(s) 471, 599, 791
DdeI CTNAG 4 cut(s) 257, 615, 698, 748
DpnI GATC 4 cut(s) 84, 309, 418, 738
DpnII GATC 4 cut(s) 82, 307, 416, 736
DraI TTTAAA 2 cut(s) 286, 645
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 141, 379, 712
EarI CTCTTC 3 cut(s) 141, 379, 712
Eco147I AGGCCT 1 cut(s) 609
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 238, 244
EcoNI CCTNNNNNAGG 2 cut(s) 341, 614
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 246, 342
EcoRV GATATC 1 cut(s) 79
FaeI CATG 2 cut(s) 314, 425
FaiI YATR 8 cut(s) 87, 143, 210, 312, 340, 423, 719, 785
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 310, 421
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 348
FokI GGATG 1 cut(s) 341
FspBI CTAG 2 cut(s) 447, 683
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 4 cut(s) 41, 43, 45, 278
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 3 cut(s) 5, 534, 609
HapII CCGG 2 cut(s) 6, 241
HgaI GACGC 2 cut(s) 65, 683
HhaI GCGC 4 cut(s) 42, 44, 46, 279
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 314, 425
Hin6I GCGC 4 cut(s) 40, 42, 44, 277
HinP1I GCGC 4 cut(s) 40, 42, 44, 277
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 4 cut(s) 20, 50, 253, 702
HpaII CCGG 2 cut(s) 6, 241
Hpy166II GTNNAC 2 cut(s) 238, 349
Hpy188I TCNGA 4 cut(s) 19, 55, 416, 741
Hpy188III TCNNGA 2 cut(s) 395, 734
Hpy8I GTNNAC 2 cut(s) 238, 349
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 255, 365
HpyCH4III ACNGT 3 cut(s) 156, 204, 766
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 4 cut(s) 257, 615, 698, 748
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 314, 425
HspAI GCGC 4 cut(s) 40, 42, 44, 277
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 4 cut(s) 82, 307, 416, 736
LguI GCTCTTC 1 cut(s) 141
MaeI CTAG 2 cut(s) 447, 683
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 198
MalI GATC 4 cut(s) 84, 309, 418, 738
MauBI CGCGCGCG 1 cut(s) 42
MboI GATC 4 cut(s) 82, 307, 416, 736
MboII GAAGA 6 cut(s) 128, 396, 633, 699, 700, 722
MluCI AATT 5 cut(s) 165, 177, 287, 526, 579
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 4 cut(s) 94, 214, 482, 620
MroXI GAANNNNTTC 2 cut(s) 13, 156
MseI TTAA 6 cut(s) 285, 290, 405, 498, 521, 644
MslI CAYNNNNRTG 1 cut(s) 732
MspI CCGG 2 cut(s) 6, 241
MspR9I CCNGG 3 cut(s) 242, 248, 344
MvaI CCWGG 2 cut(s) 248, 344
MvnI CGCG 3 cut(s) 42, 44, 46
MwoI GCNNNNNNNGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 82, 307, 416, 736
NlaIII CATG 2 cut(s) 314, 425
NlaIV GGNNCC 2 cut(s) 245, 678
NmeAIII GCCGAG 1 cut(s) 523
NmuCI GTSAC 1 cut(s) 198
NsbI TGCGCA 1 cut(s) 278
NspI RCATGY 1 cut(s) 425
PauI GCGCGC 2 cut(s) 40, 42
PceI AGGCCT 1 cut(s) 609
PciI ACATGT 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 145
PdmI GAANNNNTTC 2 cut(s) 13, 156
PfeI GAWTC 3 cut(s) 20, 50, 702
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 244
PscI ACATGT 1 cut(s) 421
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 246, 342
PspGI CCWGG 2 cut(s) 246, 342
PspN4I GGNNCC 2 cut(s) 245, 678
PspPI GGNCC 2 cut(s) 238, 244
PspPPI RGGWCCY 1 cut(s) 244
PteI GCGCGC 2 cut(s) 40, 42
RsaI GTAC 3 cut(s) 472, 600, 792
RsaNI GTAC 3 cut(s) 471, 599, 791
RseI CAYNNNNRTG 1 cut(s) 732
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 6 cut(s) 285, 290, 405, 498, 521, 644
Sau3AI GATC 4 cut(s) 82, 307, 416, 736
Sau96I GGNCC 2 cut(s) 238, 244
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 242, 248, 344
SetI ASST 9 cut(s) 34, 116, 249, 339, 348, 441, 448, 498, 754
SinI GGWCC 2 cut(s) 238, 244
SmiMI CAYNNNNRTG 1 cut(s) 732
Sse9I AATT 5 cut(s) 165, 177, 287, 526, 579
SseBI AGGCCT 1 cut(s) 609
SsiI CCGC 4 cut(s) 72, 161, 196, 649
SspI AATATT 1 cut(s) 13
SspMI CTAG 2 cut(s) 447, 683
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 240, 246, 342
TaaI ACNGT 3 cut(s) 156, 204, 766
TaiI ACGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 366
TasI AATT 5 cut(s) 165, 177, 287, 526, 579
TatI WGTACW 2 cut(s) 470, 598
TfiI GAWTC 3 cut(s) 20, 50, 702
Tru1I TTAA 6 cut(s) 285, 290, 405, 498, 521, 644
Tru9I TTAA 6 cut(s) 285, 290, 405, 498, 521, 644
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 4 cut(s) 178, 519, 539, 716
TspRI CASTG 1 cut(s) 220
VpaK11BI GGWCC 2 cut(s) 238, 244
XagI CCTNNNNNAGG 2 cut(s) 341, 614
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 2 cut(s) 13, 156
XspI CTAG 2 cut(s) 447, 683
ZrmI AGTACT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.