Rh6BG308200

Serine-threonine protein kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001830
Physical Location & Seq
Forward (+)
53720037 .. 53725581
5545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG308200.1

Sequence Viewer

Length: 615 bp
ATGGCCGGAAATATTTCAGAATCTTTGAAAGGTAAAAAGCGCGCGCGAGAATCCCACGCCGAACCCGAAACCGCCGATATCGATCATATTCTATCGTTAGAGGAAAATCTTACGTTTAGTGATACATTGGTGGCTCTTCGCATAATGCGAGAACAGTTTCCGCAAATTGACAAGCCAATCCAGCCTTTCATTTTGCGGTCACAGTTGTATAGCAGTGTAGAGGACAGGACACAAGTGGACCGGGACCTGGAGACTCTAAGAAGGGAGAAAGTGTTACGAATTTTTAAATTAAATTCGGGGCAAGATGATCATGCCATAATGTTTTTGGATGACTACCTATGCCAGGTAGACCGAGTTGTGAAAAGATTGGAAGGAGAAAAGGGAAGAGATGTTCTTGAAGTTTTTAACTGGTTCAGATCACACGTTATTGATTTCAAGCTTGAACCTAGTATTGAACACCAAGAACTTTGTACATTATTATCACTTGGAGGAAAGGTTAAAGATGAACACATCTCACTTTTAATGAATTCTGGCCTTCTTGGATTGCTTAAGTGTTGTTTGTTGGATTTCTTTCAAACAATTTTGATGGCCTTCAGCTTTCTAACTGTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

204

Amino Acids

23.55

Weight (kDa)

5.42

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 36 - 180 1.8e-12 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AccII CGCG 3 cut(s) 42, 44, 46
AciI CCGC 3 cut(s) 72, 161, 196
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 279, 292, 526
AcuI CTGAAG 1 cut(s) 577
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 247, 343
AflII CTTAAG 1 cut(s) 548
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 6 cut(s) 28, 398, 436, 443, 455, 575
AjnI CCWGG 2 cut(s) 246, 342
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 2 cut(s) 439, 597
AluI AGCT 2 cut(s) 439, 597
Alw26I GTCTC 1 cut(s) 245
AoxI GGCC 3 cut(s) 3, 532, 588
ApoI RAATTY 3 cut(s) 279, 292, 526
Asp700I GAANNNNTTC 2 cut(s) 13, 156
AspLEI GCGC 3 cut(s) 42, 44, 46
AspS9I GGNCC 2 cut(s) 238, 244
AsuC2I CCSGG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 238, 244
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BccI CCATC 1 cut(s) 580
BciT130I CCWGG 2 cut(s) 248, 344
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 447
BfrI CTTAAG 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 242, 248, 344
Bme18I GGWCC 2 cut(s) 238, 244
BmgT120I GGNCC 2 cut(s) 238, 244
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 3 cut(s) 242, 248, 344
BpmI CTGGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 81
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 480, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 343
Bse1I ACTGG 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 248, 344
BseCI ATCGAT 1 cut(s) 81
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 247, 343
BseNI ACTGG 1 cut(s) 413
BsePI GCGCGC 2 cut(s) 40, 42
Bsh1236I CGCG 3 cut(s) 42, 44, 46
BshFI GGCC 3 cut(s) 5, 534, 590
BshVI ATCGAT 1 cut(s) 81
BsiSI CCGG 2 cut(s) 6, 241
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 247, 343
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 3 cut(s) 5, 534, 590
Bsp1407I TGTACA 1 cut(s) 470
Bsp143I GATC 3 cut(s) 82, 307, 416
BspACI CCGC 3 cut(s) 72, 161, 196
BspANI GGCC 3 cut(s) 5, 534, 590
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 3 cut(s) 42, 44, 46
BspLI GGNNCC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 548
BsrGI TGTACA 1 cut(s) 470
BsrI ACTGG 1 cut(s) 413
BssHII GCGCGC 2 cut(s) 40, 42
BssMI GATC 3 cut(s) 82, 307, 416
Bst2UI CCWGG 2 cut(s) 248, 344
Bst4CI ACNGT 3 cut(s) 156, 204, 607
Bst6I CTCTTC 2 cut(s) 141, 379
BstAFI CTTAAG 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 42, 44
BstDEI CTNAG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 3 cut(s) 42, 44, 46
BstHHI GCGC 3 cut(s) 42, 44, 46
BstKTI GATC 3 cut(s) 85, 310, 419
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 3 cut(s) 82, 307, 416
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNI CCWGG 2 cut(s) 248, 344
BstSCI CCNGG 3 cut(s) 240, 246, 342
BstUI CGCG 3 cut(s) 42, 44, 46
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 3 cut(s) 5, 534, 590
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 2 cut(s) 42, 44
CfoI GCGC 3 cut(s) 42, 44, 46
Cfr13I GGNCC 2 cut(s) 238, 244
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 1 cut(s) 471
CviAII CATG 2 cut(s) 311, 612
CviJI RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 590, 597
CviKI_1 RGCY 8 cut(s) 5, 134, 175, 184, 439, 534, 590, 597
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 1 cut(s) 257
DpnI GATC 3 cut(s) 84, 309, 418
DpnII GATC 3 cut(s) 82, 307, 416
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 141, 379
EarI CTCTTC 2 cut(s) 141, 379
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 238, 244
Eco57I CTGAAG 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 246, 342
EcoRV GATATC 1 cut(s) 79
FaeI CATG 2 cut(s) 314, 615
FaiI YATR 7 cut(s) 87, 143, 210, 312, 317, 340, 613
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 310, 611
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 1 cut(s) 348
FokI GGATG 1 cut(s) 341
FspBI CTAG 1 cut(s) 447
GlaI GCGC 3 cut(s) 41, 43, 45
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 3 cut(s) 5, 534, 590
HapII CCGG 2 cut(s) 6, 241
HhaI GCGC 3 cut(s) 42, 44, 46
Hin1II CATG 2 cut(s) 314, 615
Hin6I GCGC 3 cut(s) 40, 42, 44
HinP1I GCGC 3 cut(s) 40, 42, 44
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 20, 50, 253
HpaII CCGG 2 cut(s) 6, 241
Hpy166II GTNNAC 2 cut(s) 238, 349
Hpy188I TCNGA 2 cut(s) 19, 416
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 2 cut(s) 238, 349
HpyAV CCTTC 4 cut(s) 255, 365, 545, 601
HpyCH4III ACNGT 3 cut(s) 156, 204, 607
HpyCH4IV ACGT 2 cut(s) 113, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 113, 423
Hsp92II CATG 2 cut(s) 314, 615
HspAI GCGC 3 cut(s) 40, 42, 44
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 82, 307, 416
LguI GCTCTTC 1 cut(s) 141
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 113, 423
MaeIII GTNAC 3 cut(s) 198, 273, 607
MalI GATC 3 cut(s) 84, 309, 418
MauBI CGCGCGCG 1 cut(s) 42
MboI GATC 3 cut(s) 82, 307, 416
MboII GAAGA 2 cut(s) 128, 396
MluCI AATT 6 cut(s) 165, 279, 287, 292, 526, 579
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 3 cut(s) 94, 214, 482
MroXI GAANNNNTTC 2 cut(s) 13, 156
MseI TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 2 cut(s) 6, 241
MspR9I CCNGG 3 cut(s) 242, 248, 344
MvaI CCWGG 2 cut(s) 248, 344
MvnI CGCG 3 cut(s) 42, 44, 46
MwoI GCNNNNNNNGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 242
NdeII GATC 3 cut(s) 82, 307, 416
NlaIII CATG 2 cut(s) 314, 615
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 2 cut(s) 198, 607
PauI GCGCGC 2 cut(s) 40, 42
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 145
PdmI GAANNNNTTC 2 cut(s) 13, 156
PfeI GAWTC 2 cut(s) 20, 50
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 246, 342
PspGI CCWGG 2 cut(s) 246, 342
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 2 cut(s) 238, 244
PspPPI RGGWCCY 1 cut(s) 244
PteI GCGCGC 2 cut(s) 40, 42
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
Sau3AI GATC 3 cut(s) 82, 307, 416
Sau96I GGNCC 2 cut(s) 238, 244
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 242, 248, 344
SinI GGWCC 2 cut(s) 238, 244
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 6 cut(s) 165, 279, 287, 292, 526, 579
SsiI CCGC 3 cut(s) 72, 161, 196
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 240, 246, 342
TaaI ACNGT 3 cut(s) 156, 204, 607
TaiI ACGT 2 cut(s) 116, 426
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 366
TasI AATT 6 cut(s) 165, 279, 287, 292, 526, 579
TatI WGTACW 1 cut(s) 470
TfiI GAWTC 2 cut(s) 20, 50
Tru1I TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
Tru9I TTAA 6 cut(s) 285, 290, 405, 498, 521, 549
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 198, 607
Tsp45I GTSAC 2 cut(s) 198, 607
TspDTI ATGAA 3 cut(s) 178, 519, 539
TspRI CASTG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 238, 244
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 3 cut(s) 279, 292, 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 2 cut(s) 13, 156
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.