MD05G1195200.v1.1

Serine-threonine protein kinase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001830
Physical Location & Seq
Forward (+)
32241746 .. 32245352
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1195200.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGCGACTATTTCAGATTCTTTGAAAGGTAAAAAACGCCCGCGAGAGACAGACGCTGAAGCCGCAACCGGCGACTTCGATCAAATTCTATCACTAGAGGAAAATCTTACATTCAGCGACACGTTGGTGGCGCTTCGGATAATGCGAGCTCAGTTTCCGAACAACGAAAAGGTAACGGTTCAGCCTTTCATTTTGCGGTCACAGCTGTATAGCAGTGTGGAGGATCGAACACAAGTGGACCGGGATCTAGAGACTCTAAAAAGAGAGAAAGTGCTACGGGTTTTTAAATTAAATACTGGACAAGACGATCATGCAATAATGTTTTTGGATGACTATTTTCGCCAGATGGAAAAAGTTGTGAAAAGATTCGAAGAAAAGGACGAAGATATTGTTGAAGTTTTTAAGTGGTTTAAATCACACGTTATTGATTGCAAGCTTGAACCCAGTATTGGACATCAAGATCTTTGTACATTATTATCTCGTGGAGGAAAGGTGGAGGATGAACACATCTCCCTTTTGATCAATTCTGGCCTTCTTACTCGTCAACTTATTGATCCAAACGTTTACTGGTTCGCAATTCCAAATATTGGTTCAGTACTAAAAGGCCTCTCTCAGGGAAGAAAGGAAGTTCTCTCTCTTCTAAACCGGAGGAGATACAAAGAAATGATGATGGCCCCTTTAGAGAAGAAGGCTCTTCGATTTTCTCCGCTTGATATGAGATTTCACCTCCGTGATCTGATAGGCTCAGGCCATCTCAAAACTTCCAAGACACCGACAGGCTTAGTTGTTCGAGTCTCAAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stk19

Protein Analysis

269

Amino Acids

30.91

Weight (kDa)

8.73

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stk19 PF10494 29 - 265 1.8e-32 Inactive serine-threonine protein kinase 19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 587
AccII CGCG 1 cut(s) 43
AciI CCGC 4 cut(s) 41, 63, 196, 707
AclI AACGTT 1 cut(s) 561
AclWI GGATC 3 cut(s) 231, 252, 548
AcsI RAATTY 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 2 cut(s) 469, 597
AfiI CCNNNNNNNGG 3 cut(s) 449, 587, 613
AflIII ACRYGT 2 cut(s) 120, 418
AgsI TTSAA 3 cut(s) 25, 395, 440
AjuI GAANNNNNNNTTGG 4 cut(s) 432, 464, 574, 606
AleI CACNNNNGTG 2 cut(s) 125, 729
AluBI AGCT 3 cut(s) 149, 205, 436
AluI AGCT 3 cut(s) 149, 205, 436
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 3 cut(s) 41, 245, 799
AlwI GGATC 3 cut(s) 231, 252, 548
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 4 cut(s) 529, 604, 672, 748
ApoI RAATTY 1 cut(s) 84
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 238, 673
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 716
AsuII TTCGAA 1 cut(s) 369
AvaII GGWCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 151
BauI CACGAG 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 151
BccI CCATC 3 cut(s) 340, 664, 759
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 3 cut(s) 41, 245, 799
BfaI CTAG 2 cut(s) 95, 248
BfoI RGCGCY 1 cut(s) 134
BglII AGATCT 1 cut(s) 460
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmcAI AGTACT 1 cut(s) 597
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 238, 673
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 1 cut(s) 242
BmrI ACTGGG 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 745
Bpu14I TTCGAA 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 587, 613
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 3 cut(s) 301, 444, 572
BseGI GGATG 2 cut(s) 334, 505
BseLI CCNNNNNNNGG 3 cut(s) 449, 587, 613
BseMII CTCAG 3 cut(s) 164, 626, 759
BseNI ACTGG 3 cut(s) 301, 444, 572
BseRI GAGGAG 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 43
BshFI GGCC 4 cut(s) 531, 606, 674, 750
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 3 cut(s) 69, 241, 646
BslI CCNNNNNNNGG 3 cut(s) 449, 587, 613
BsmAI GTCTC 3 cut(s) 41, 245, 799
BsnI GGCC 4 cut(s) 531, 606, 674, 750
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 467
Bsp143I GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
BspACI CCGC 4 cut(s) 41, 63, 196, 707
BspANI GGCC 4 cut(s) 531, 606, 674, 750
BspCNI CTCAG 3 cut(s) 163, 625, 758
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 675
BspPI GGATC 3 cut(s) 231, 252, 548
BspQI GCTCTTC 1 cut(s) 699
BspT104I TTCGAA 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 467
BsrI ACTGG 3 cut(s) 301, 444, 572
BssAI RCCGGY 1 cut(s) 68
BssMI GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
BssSI CACGAG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 2 cut(s) 642, 699
BstAUI TGTACA 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 3 cut(s) 41, 147, 434
BstDEI CTNAG 4 cut(s) 150, 612, 745, 781
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 2 cut(s) 334, 505
BstFNI CGCG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 8 cut(s) 82, 226, 247, 310, 463, 522, 556, 736
BstMAI GTCTC 3 cut(s) 41, 245, 799
BstMBI GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
BstMWI GCNNNNNNNGC 2 cut(s) 62, 202
BstSCI CCNGG 1 cut(s) 240
BstUI CGCG 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 244, 460
BstYI RGATCY 2 cut(s) 244, 460
BsuRI GGCC 4 cut(s) 531, 606, 674, 750
BtsCI GGATG 2 cut(s) 334, 505
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 3 cut(s) 41, 147, 434
CaiI CAGNNNCTG 1 cut(s) 56
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 238, 673
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 468, 596
CviAII CATG 1 cut(s) 311
CviQI GTAC 2 cut(s) 468, 596
DdeI CTNAG 4 cut(s) 150, 612, 745, 781
DpnI GATC 8 cut(s) 81, 225, 246, 309, 462, 521, 555, 735
DpnII GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
DraI TTTAAA 2 cut(s) 286, 412
Eam1104I CTCTTC 2 cut(s) 642, 699
EarI CTCTTC 2 cut(s) 642, 699
Ecl136II GAGCTC 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 149
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoT38I GRGCYC 1 cut(s) 151
FaeI CATG 1 cut(s) 314
FaiI YATR 3 cut(s) 210, 312, 716
FatI CATG 1 cut(s) 310
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 2 cut(s) 341, 512
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 95, 248
GlaI GCGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 134
HaeIII GGCC 4 cut(s) 531, 606, 674, 750
HapII CCGG 3 cut(s) 69, 241, 646
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 133
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 545
HindII GTYRAC 1 cut(s) 545
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 4 cut(s) 17, 253, 366, 792
HpaII CCGG 3 cut(s) 69, 241, 646
HphI GGTGA 1 cut(s) 716
Hpy166II GTNNAC 3 cut(s) 238, 545, 565
Hpy188I TCNGA 4 cut(s) 16, 138, 159, 738
Hpy188III TCNNGA 2 cut(s) 248, 458
Hpy8I GTNNAC 3 cut(s) 238, 545, 565
HpyAV CCTTC 2 cut(s) 542, 682
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 3 cut(s) 122, 420, 561
HpyCH4V TGCA 2 cut(s) 314, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 202
HpyF3I CTNAG 4 cut(s) 150, 612, 745, 781
HpySE526I ACGT 3 cut(s) 122, 420, 561
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 1 cut(s) 131
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
LguI GCTCTTC 1 cut(s) 699
MaeI CTAG 2 cut(s) 95, 248
MaeII ACGT 3 cut(s) 122, 420, 561
MaeIII GTNAC 2 cut(s) 172, 198
MalI GATC 8 cut(s) 81, 225, 246, 309, 462, 521, 555, 735
MboI GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
MboII GAAGA 6 cut(s) 383, 395, 629, 630, 686, 697
MflI RGATCY 2 cut(s) 244, 460
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 4 cut(s) 84, 287, 523, 576
MlyI GAGTC 2 cut(s) 247, 801
MnlI CCTC 7 cut(s) 91, 214, 479, 490, 617, 642, 737
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 4 cut(s) 285, 290, 402, 411
MslI CAYNNNNRTG 2 cut(s) 125, 729
MspA1I CMGCKG 1 cut(s) 205
MspI CCGG 3 cut(s) 69, 241, 646
MspR9I CCNGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 2 cut(s) 62, 202
NciI CCSGG 1 cut(s) 242
NdeII GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 1 cut(s) 675
NmuCI GTSAC 1 cut(s) 198
NspV TTCGAA 1 cut(s) 369
OliI CACNNNNGTG 2 cut(s) 125, 729
PceI AGGCCT 1 cut(s) 606
PciSI GCTCTTC 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 17, 366
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 2 cut(s) 247, 800
PpsI GAGTC 2 cut(s) 247, 800
Psp124BI GAGCTC 1 cut(s) 151
Psp1406I AACGTT 1 cut(s) 561
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 2 cut(s) 238, 673
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 2 cut(s) 244, 460
PvuII CAGCTG 1 cut(s) 205
RsaI GTAC 2 cut(s) 469, 597
RsaNI GTAC 2 cut(s) 468, 596
RseI CAYNNNNRTG 2 cut(s) 125, 729
SacI GAGCTC 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 699
SaqAI TTAA 4 cut(s) 285, 290, 402, 411
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 8 cut(s) 79, 223, 244, 307, 460, 519, 553, 733
Sau96I GGNCC 2 cut(s) 238, 673
ScaI AGTACT 1 cut(s) 597
SchI GAGTC 2 cut(s) 247, 801
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 2 cut(s) 125, 729
Sse9I AATT 4 cut(s) 84, 287, 523, 576
SseBI AGGCCT 1 cut(s) 606
SsiI CCGC 4 cut(s) 41, 63, 196, 707
SspI AATATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 95, 248
SstI GAGCTC 1 cut(s) 151
StuI AGGCCT 1 cut(s) 606
StyD4I CCNGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 3 cut(s) 125, 423, 564
TaqI TCGA 5 cut(s) 78, 226, 369, 697, 790
TasI AATT 4 cut(s) 84, 287, 523, 576
TatI WGTACW 2 cut(s) 467, 595
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 17, 366
Tru1I TTAA 4 cut(s) 285, 290, 402, 411
Tru9I TTAA 4 cut(s) 285, 290, 402, 411
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 2 cut(s) 178, 516
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 2 cut(s) 95, 248
ZrmI AGTACT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.