AT2G20515

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014249
Physical Location & Seq
Reverse (-)
8838750 .. 8839857
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20515.1

Sequence Viewer

Length: 468 bp
ATGCATATCATGGACACACTTTGTTCTCCGAGTCGACTCGTTCTGGTCACCTTCTTCCTCCTCTGCTTCTTTGCCGGAGATTCATCGGCGACGAGGCCTGGTTTCATCTACACCAGACACAGAGGACGATGCACGCCGCAGTATTGGAGCAGCCAGAGGGAAGCATGGCCGAGGATGGTACCTGAGAGATCAACCGTGGAGAAAATATTCGGAGTGATGGTAGCAAAAGAACGGTGGAGATCTGATCTAACGCTAGTGGAATCAACGGCTAGGAATGACGAAGAAGGGAACGCGTACGGTGCATTGCTGAAACAGGGGATCGCAGCTCTGATCAACTCTTACGCGAGAAAAAGTTTCTCTTATGCACCATGGGAAGTGAAGACGATGCTGATCCAAGCCATGGTTTCGGAGTCGGCGGCTCGCCGGCAAGCCGAACATTTCGCCGTCGCTAACGTTGCTTGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.56

Weight (kDa)

9.02

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 178
AccB7I CCANNNNNTGG 1 cut(s) 400
AccI GTMKAC 1 cut(s) 34
AccII CGCG 2 cut(s) 293, 344
AciI CCGC 2 cut(s) 137, 416
AclI AACGTT 1 cut(s) 453
AclWI GGATC 2 cut(s) 326, 385
AcoI YGGCCR 1 cut(s) 167
AfaI GTAC 2 cut(s) 180, 296
AfiI CCNNNNNNNGG 1 cut(s) 400
AflIII ACRYGT 1 cut(s) 291
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
AlwI GGATC 2 cut(s) 326, 385
AoxI GGCC 2 cut(s) 95, 167
ApeKI GCWGC 2 cut(s) 150, 323
Asp700I GAANNNNTTC 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 40
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 162, 335
BccI CCATC 2 cut(s) 169, 211
BceAI ACGGC 2 cut(s) 282, 428
BciT130I CCWGG 1 cut(s) 99
BclI TGATCA 1 cut(s) 330
BfaI CTAG 2 cut(s) 254, 270
BglII AGATCT 1 cut(s) 239
BisI GCNGC 4 cut(s) 137, 151, 324, 417
BlsI GCNGC 4 cut(s) 138, 152, 325, 418
Bme1390I CCNGG 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 119, 375
BpiI GAAGAC 1 cut(s) 386
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 170, 195, 368, 399
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 400
Bse118I RCCGGY 1 cut(s) 423
Bse3DI GCAATG 1 cut(s) 302
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 170, 195, 368, 399
BseGI GGATG 1 cut(s) 180
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 162, 335
Bsh1236I CGCG 2 cut(s) 293, 344
BshFI GGCC 2 cut(s) 97, 169
BshNI GGYRCC 1 cut(s) 178
BsiSI CCGG 2 cut(s) 75, 424
BsiWI CGTACG 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 400
BsnI GGCC 2 cut(s) 97, 169
Bsp143I GATC 6 cut(s) 188, 239, 244, 318, 330, 390
Bsp19I CCATGG 2 cut(s) 368, 399
BspACI CCGC 2 cut(s) 137, 416
BspANI GGCC 2 cut(s) 97, 169
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 2 cut(s) 293, 344
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 2 cut(s) 326, 385
BspT107I GGYRCC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 423
BssAI RCCGGY 1 cut(s) 423
BssECI CCNNGG 4 cut(s) 170, 195, 368, 399
BssMI GATC 6 cut(s) 188, 239, 244, 318, 330, 390
BssT1I CCWWGG 2 cut(s) 368, 399
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 3 cut(s) 196, 234, 299
BstC8I GCNNGC 4 cut(s) 134, 421, 425, 429
BstDEI CTNAG 1 cut(s) 183
BstDSI CCRYGG 3 cut(s) 195, 368, 399
BstEII GGTNACC 1 cut(s) 46
BstF5I GGATG 1 cut(s) 180
BstFNI CGCG 2 cut(s) 293, 344
BstKTI GATC 6 cut(s) 191, 242, 247, 321, 333, 393
BstMBI GATC 6 cut(s) 188, 239, 244, 318, 330, 390
BstMWI GCNNNNNNNGC 2 cut(s) 299, 455
BstNI CCWGG 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 97
BstUI CGCG 2 cut(s) 293, 344
BstV1I GCAGC 2 cut(s) 162, 335
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 2 cut(s) 97, 169
BtgI CCRYGG 3 cut(s) 195, 368, 399
BtsCI GGATG 1 cut(s) 180
Cac8I GCNNGC 4 cut(s) 134, 421, 425, 429
Cfr10I RCCGGY 1 cut(s) 423
Csp6I GTAC 2 cut(s) 179, 295
CviAII CATG 4 cut(s) 10, 165, 369, 400
CviJI RGCY 8 cut(s) 97, 153, 169, 269, 326, 398, 419, 431
CviKI_1 RGCY 8 cut(s) 97, 153, 169, 269, 326, 398, 419, 431
CviQI GTAC 2 cut(s) 179, 295
DdeI CTNAG 1 cut(s) 183
DpnI GATC 6 cut(s) 190, 241, 246, 320, 332, 392
DpnII GATC 6 cut(s) 188, 239, 244, 318, 330, 390
EaeI YGGCCR 1 cut(s) 167
Eco130I CCWWGG 2 cut(s) 368, 399
Eco147I AGGCCT 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 368, 399
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 368, 399
FaeI CATG 4 cut(s) 13, 168, 372, 403
FaiI YATR 6 cut(s) 6, 11, 166, 363, 370, 401
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FatI CATG 4 cut(s) 9, 164, 368, 399
FbaI TGATCA 1 cut(s) 330
FblI GTMKAC 1 cut(s) 34
Fnu4HI GCNGC 4 cut(s) 137, 151, 324, 417
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 137, 151, 324, 417
FspBI CTAG 2 cut(s) 254, 270
GluI GCNGC 4 cut(s) 137, 151, 324, 417
HaeIII GGCC 2 cut(s) 97, 169
HapII CCGG 2 cut(s) 75, 424
Hin1II CATG 4 cut(s) 13, 168, 372, 403
HincII GTYRAC 1 cut(s) 35
HindII GTYRAC 1 cut(s) 35
HinfI GANTC 5 cut(s) 31, 36, 80, 260, 410
HpaII CCGG 2 cut(s) 75, 424
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 35
Hpy188I TCNGA 5 cut(s) 30, 212, 244, 330, 409
Hpy8I GTNNAC 1 cut(s) 35
Hpy99I CGWCG 2 cut(s) 94, 449
HpyAV CCTTC 2 cut(s) 61, 278
HpyCH4III ACNGT 3 cut(s) 196, 234, 299
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 4, 132, 302, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 455
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 4 cut(s) 13, 168, 372, 403
KpnI GGTACC 1 cut(s) 182
KroI GCCGGC 1 cut(s) 423
KroNI GCCGGC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 6 cut(s) 188, 239, 244, 318, 330, 390
LmnI GCTCC 1 cut(s) 147
LpnPI CCDG 9 cut(s) 29, 84, 88, 111, 127, 167, 195, 299, 437
Lsp1109I GCAGC 2 cut(s) 162, 335
LweI GCATC 2 cut(s) 119, 375
MaeI CTAG 2 cut(s) 254, 270
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 46, 461
MalI GATC 6 cut(s) 190, 241, 246, 320, 332, 392
MboI GATC 6 cut(s) 188, 239, 244, 318, 330, 390
MboII GAAGA 3 cut(s) 46, 293, 391
MflI RGATCY 1 cut(s) 239
MluI ACGCGT 1 cut(s) 291
MlyI GAGTC 3 cut(s) 30, 40, 419
MnlI CCTC 6 cut(s) 68, 71, 87, 116, 150, 165
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 423
MroXI GAANNNNTTC 1 cut(s) 206
MspI CCGG 2 cut(s) 75, 424
MspR9I CCNGG 1 cut(s) 99
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 2 cut(s) 293, 344
MwoI GCNNNNNNNGC 2 cut(s) 299, 455
NaeI GCCGGC 1 cut(s) 425
NcoI CCATGG 2 cut(s) 368, 399
NdeII GATC 6 cut(s) 188, 239, 244, 318, 330, 390
NgoMIV GCCGGC 1 cut(s) 423
NlaIII CATG 4 cut(s) 13, 168, 372, 403
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 46, 461
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 97
PdiI GCCGGC 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 2 cut(s) 80, 260
Pfl23II CGTACG 1 cut(s) 294
PflMI CCANNNNNTGG 1 cut(s) 400
PkrI GCNGC 4 cut(s) 138, 152, 325, 418
PleI GAGTC 3 cut(s) 30, 39, 418
PpsI GAGTC 3 cut(s) 30, 39, 418
Psp1406I AACGTT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 97
PspEI GGTNACC 1 cut(s) 46
PspGI CCWGG 1 cut(s) 97
PspLI CGTACG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 180
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 2 cut(s) 180, 296
RsaNI GTAC 2 cut(s) 179, 295
SalI GTCGAC 1 cut(s) 33
SatI GCNGC 4 cut(s) 137, 151, 324, 417
Sau3AI GATC 6 cut(s) 188, 239, 244, 318, 330, 390
SchI GAGTC 3 cut(s) 30, 40, 419
ScrFI CCNGG 1 cut(s) 99
SetI ASST 4 cut(s) 53, 184, 328, 456
SfaNI GCATC 2 cut(s) 119, 375
SseBI AGGCCT 1 cut(s) 97
SsiI CCGC 2 cut(s) 137, 416
SspI AATATT 1 cut(s) 207
SspMI CTAG 2 cut(s) 254, 270
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 2 cut(s) 368, 399
TaaI ACNGT 3 cut(s) 196, 234, 299
TaiI ACGT 1 cut(s) 456
TaqI TCGA 1 cut(s) 34
TauI GCSGC 2 cut(s) 139, 419
TfiI GAWTC 2 cut(s) 80, 260
TseFI GTSAC 2 cut(s) 46, 461
TseI GCWGC 2 cut(s) 150, 323
Tsp45I GTSAC 2 cut(s) 46, 461
TspDTI ATGAA 2 cut(s) 72, 94
Van91I CCANNNNNTGG 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 254, 270
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.