FvH4_2g21450

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014249
Physical Location & Seq
Forward (+)
17832756 .. 17834079
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21450.t1

Sequence Viewer

Length: 444 bp
ATGGCTCGTCCTTCTCAACTGACTCACCTCACTCTTCTTCTTCTTCTTCTAGCTACAACCGCAACAGCCAGGCCAGGCTTTCTCTTCACTCGAACCACTGGGCGGTGCACGCCGGACTACTGGGGCAGCAGAGTGGAGTCATGGCCGAGGATGGTCCCGCAGACCTCGACGGTGTCCAAGGTGTTCGGGTCCAGAGCCTACGAACGTTACAGATCCGATCTGACGCTGATGGAAGCGACGGCCAGGAACGACGATGAGAACGTGTTCCCTAGGCTGCTCAAGGAGGCGAGCGCGGCGCTGATCAACTCGTACGCCAGGAAAGGGTTCCCGTACACGGCGTGGGAGGTGAAGACGAGGGTCATTCAGGGCTTGGTGTCGGAGAGGGCGGCGGCCCGTCAGGCCCAGGTTTTCTTTCAGGCCAATCGGGCTTGTGATCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.63

Weight (kDa)

10.09

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 293
AciI CCGC 6 cut(s) 60, 103, 158, 293, 386, 389
AclI AACGTT 1 cut(s) 205
AclWI GGATC 1 cut(s) 207
AcoI YGGCCR 2 cut(s) 143, 240
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 2 cut(s) 311, 332
AfiI CCNNNNNNNGG 3 cut(s) 102, 321, 334
AflIII ACRYGT 1 cut(s) 261
AjnI CCWGG 5 cut(s) 68, 73, 242, 314, 402
AluBI AGCT 1 cut(s) 53
AluI AGCT 1 cut(s) 53
Alw21I GWGCWC 1 cut(s) 110
Alw44I GTGCAC 1 cut(s) 106
AlwI GGATC 1 cut(s) 207
AoxI GGCC 6 cut(s) 71, 143, 240, 390, 399, 417
ApaLI GTGCAC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 126, 274
Asp700I GAANNNNTTC 2 cut(s) 263, 323
AspA2I CCTAGG 1 cut(s) 269
AspLEI GCGC 2 cut(s) 293, 298
AspS9I GGNCC 4 cut(s) 154, 189, 391, 400
AsuHPI GGTGA 2 cut(s) 17, 358
AvaII GGWCC 2 cut(s) 154, 189
AvrII CCTAGG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 138, 261
BccI CCATC 2 cut(s) 145, 223
BceAI ACGGC 2 cut(s) 255, 351
BciT130I CCWGG 5 cut(s) 70, 75, 244, 316, 404
BclI TGATCA 1 cut(s) 300
BfaI CTAG 3 cut(s) 50, 270, 442
BfoI RGCGCY 1 cut(s) 299
BglI GCCNNNNNGGC 2 cut(s) 398, 425
BisI GCNGC 5 cut(s) 127, 275, 294, 387, 390
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 5 cut(s) 128, 276, 295, 388, 391
Bme1390I CCNGG 5 cut(s) 70, 75, 244, 316, 404
Bme18I GGWCC 2 cut(s) 154, 189
BmgT120I GGNCC 4 cut(s) 154, 189, 391, 400
BmiI GGNNCC 3 cut(s) 156, 190, 326
BmrFI CCNGG 5 cut(s) 70, 75, 244, 316, 404
BmrI ACTGGG 2 cut(s) 108, 130
BmuI ACTGGG 2 cut(s) 108, 130
BoxI GACNNNNGTC 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 146, 177, 269, 402
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 321, 334
Bse1I ACTGG 2 cut(s) 103, 125
BseBI CCWGG 5 cut(s) 70, 75, 244, 316, 404
BseDI CCNNGG 4 cut(s) 146, 177, 269, 402
BseGI GGATG 1 cut(s) 156
BseLI CCNNNNNNNGG 3 cut(s) 102, 321, 334
BseNI ACTGG 2 cut(s) 103, 125
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 2 cut(s) 138, 261
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 6 cut(s) 73, 145, 242, 392, 401, 419
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 1 cut(s) 113
BsiWI CGTACG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 3 cut(s) 102, 321, 334
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 6 cut(s) 73, 145, 242, 392, 401, 419
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 4 cut(s) 212, 217, 300, 433
BspACI CCGC 6 cut(s) 60, 103, 158, 293, 386, 389
BspANI GGCC 6 cut(s) 73, 145, 242, 392, 401, 419
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 156, 190, 326
BspPI GGATC 1 cut(s) 207
BsrI ACTGG 2 cut(s) 103, 125
BssECI CCNNGG 4 cut(s) 146, 177, 269, 402
BssMI GATC 4 cut(s) 212, 217, 300, 433
BssT1I CCWWGG 2 cut(s) 177, 269
Bst2UI CCWGG 5 cut(s) 70, 75, 244, 316, 404
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 39, 89
BstC8I GCNNGC 2 cut(s) 110, 289
BstF5I GGATG 1 cut(s) 156
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 293, 298
BstKTI GATC 4 cut(s) 215, 220, 303, 436
BstMBI GATC 4 cut(s) 212, 217, 300, 433
BstMWI GCNNNNNNNGC 5 cut(s) 59, 109, 293, 398, 425
BstNI CCWGG 5 cut(s) 70, 75, 244, 316, 404
BstPAI GACNNNNGTC 1 cut(s) 356
BstSCI CCNGG 5 cut(s) 68, 73, 242, 314, 402
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 138, 261
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuRI GGCC 6 cut(s) 73, 145, 242, 392, 401, 419
BtsCI GGATG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 110, 289
CfoI GCGC 2 cut(s) 293, 298
Cfr13I GGNCC 4 cut(s) 154, 189, 391, 400
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 310, 331
CviAII CATG 1 cut(s) 141
CviQI GTAC 2 cut(s) 310, 331
DpnI GATC 4 cut(s) 214, 219, 302, 435
DpnII GATC 4 cut(s) 212, 217, 300, 433
DraIII CACNNNGTG 1 cut(s) 339
EaeI YGGCCR 2 cut(s) 143, 240
Eam1104I CTCTTC 2 cut(s) 39, 89
EarI CTCTTC 2 cut(s) 39, 89
Eco130I CCWWGG 2 cut(s) 177, 269
Eco47I GGWCC 2 cut(s) 154, 189
EcoRII CCWGG 5 cut(s) 68, 73, 242, 314, 402
EcoT14I CCWWGG 2 cut(s) 177, 269
ErhI CCWWGG 2 cut(s) 177, 269
FaeI CATG 1 cut(s) 144
FaiI YATR 1 cut(s) 142
FaqI GGGAC 1 cut(s) 140
FatI CATG 1 cut(s) 140
FauI CCCGC 1 cut(s) 165
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 127, 275, 294, 387, 390
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 5 cut(s) 127, 275, 294, 387, 390
FspBI CTAG 3 cut(s) 50, 270, 442
GlaI GCGC 2 cut(s) 292, 297
GluI GCNGC 5 cut(s) 127, 275, 294, 387, 390
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 6 cut(s) 73, 145, 242, 392, 401, 419
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 232
HhaI GCGC 2 cut(s) 293, 298
Hin1II CATG 1 cut(s) 144
Hin6I GCGC 2 cut(s) 291, 296
HinP1I GCGC 2 cut(s) 291, 296
HinfI GANTC 2 cut(s) 22, 137
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 17, 358
Hpy166II GTNNAC 2 cut(s) 108, 333
Hpy188I TCNGA 3 cut(s) 217, 222, 379
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 2 cut(s) 108, 333
Hpy99I CGWCG 3 cut(s) 172, 241, 254
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 205, 261
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 109, 293, 398, 425
HpySE526I ACGT 2 cut(s) 205, 261
Hsp92II CATG 1 cut(s) 144
HspAI GCGC 2 cut(s) 291, 296
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 4 cut(s) 212, 217, 300, 433
Lsp1109I GCAGC 2 cut(s) 138, 261
MaeI CTAG 3 cut(s) 50, 270, 442
MaeII ACGT 2 cut(s) 205, 261
MaeIII GTNAC 1 cut(s) 206
MalI GATC 4 cut(s) 214, 219, 302, 435
MboI GATC 4 cut(s) 212, 217, 300, 433
MboII GAAGA 7 cut(s) 26, 29, 32, 35, 38, 76, 361
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 110
MlyI GAGTC 2 cut(s) 16, 146
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 7 cut(s) 38, 141, 175, 277, 337, 348, 375
MroXI GAANNNNTTC 2 cut(s) 263, 323
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 5 cut(s) 70, 75, 244, 316, 404
MteI GCGCNGCGC 1 cut(s) 294
MvaI CCWGG 5 cut(s) 70, 75, 244, 316, 404
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 5 cut(s) 59, 109, 293, 398, 425
NdeII GATC 4 cut(s) 212, 217, 300, 433
NlaIII CATG 1 cut(s) 144
NlaIV GGNNCC 3 cut(s) 156, 190, 326
NmeAIII GCCGAG 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 2 cut(s) 263, 323
Pfl23II CGTACG 1 cut(s) 309
PflFI GACNNNGTC 1 cut(s) 172
PkrI GCNGC 5 cut(s) 128, 276, 295, 388, 391
PleI GAGTC 2 cut(s) 16, 145
PpsI GAGTC 2 cut(s) 16, 145
PshAI GACNNNNGTC 1 cut(s) 356
Psp1406I AACGTT 1 cut(s) 205
Psp6I CCWGG 5 cut(s) 68, 73, 242, 314, 402
PspGI CCWGG 5 cut(s) 68, 73, 242, 314, 402
PspLI CGTACG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 156, 190, 326
PspPI GGNCC 4 cut(s) 154, 189, 391, 400
PsuI RGATCY 1 cut(s) 212
PsyI GACNNNGTC 1 cut(s) 172
RsaI GTAC 2 cut(s) 311, 332
RsaNI GTAC 2 cut(s) 310, 331
SatI GCNGC 5 cut(s) 127, 275, 294, 387, 390
Sau3AI GATC 4 cut(s) 212, 217, 300, 433
Sau96I GGNCC 4 cut(s) 154, 189, 391, 400
SchI GAGTC 2 cut(s) 16, 146
ScrFI CCNGG 5 cut(s) 70, 75, 244, 316, 404
SduI GDGCHC 1 cut(s) 110
SetI ASST 8 cut(s) 30, 55, 167, 183, 208, 264, 348, 408
SfiI GGCCNNNNNGGCC 1 cut(s) 398
SinI GGWCC 2 cut(s) 154, 189
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SsiI CCGC 6 cut(s) 60, 103, 158, 293, 386, 389
SspMI CTAG 3 cut(s) 50, 270, 442
StyD4I CCNGG 5 cut(s) 68, 73, 242, 314, 402
StyI CCWWGG 2 cut(s) 177, 269
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 208, 264
TaqI TCGA 2 cut(s) 91, 167
TauI GCSGC 3 cut(s) 296, 389, 392
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 126, 274
TspRI CASTG 1 cut(s) 103
Tth111I GACNNNGTC 1 cut(s) 172
VneI GTGCAC 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 154, 189
XmaJI CCTAGG 1 cut(s) 269
XmnI GAANNNNTTC 2 cut(s) 263, 323
XspI CTAG 3 cut(s) 50, 270, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.