Rw6G026330

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014249
Physical Location & Seq
Forward (+)
49684331 .. 49684777
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026330.1

Sequence Viewer

Length: 417 bp
ATGACTCGTCCTTTTCAACTGAGTCACCTCCTTCTTCTTCTTCTTCTTCTTCTAGCTACTACCGCAACAGCCAGGCCGGGCTTTCTCTTCACCCGGACCACCGGCCGGTGCACACCGGACTACTGGGGCAGCCGGACATCGACGGTGTCCAAGGTGTTCGGGTCCAGAGCCTACGAACGGTACAGATCCGATCTGACGCTGCTGGAAGCGACGGCAAGGAGCGACGACGAGAACGTCTTCCCTAGGCTGCTGAAGCAGGCGAGCGCGGCGCTGCTCAACTCGTACTCCAGGAAGGGATTCCCGTACACGGCGTGGGAGGTGAAGACTCGGGTCATACAGGGCTTGGTGTCCGAGAGGGCGGCGTCCCGTCAGGCCCAGCTATTCTCTCAGGCCAATGAGGCTTGTGATCTGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.53

Weight (kDa)

9.92

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccII CGCG 1 cut(s) 266
AciI CCGC 3 cut(s) 63, 266, 359
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 362
AdeI CACNNNGTG 1 cut(s) 312
AfaI GTAC 3 cut(s) 182, 284, 305
AfiI CCNNNNNNNGG 3 cut(s) 105, 177, 307
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 2 cut(s) 71, 287
AluBI AGCT 2 cut(s) 56, 379
AluI AGCT 2 cut(s) 56, 379
Alw21I GWGCWC 1 cut(s) 113
Alw44I GTGCAC 1 cut(s) 109
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 4 cut(s) 74, 103, 372, 390
ApaLI GTGCAC 1 cut(s) 109
ApeKI GCWGC 4 cut(s) 129, 199, 247, 271
Asp700I GAANNNNTTC 2 cut(s) 236, 296
AspA2I CCTAGG 1 cut(s) 242
AspLEI GCGC 2 cut(s) 266, 271
AspS9I GGNCC 3 cut(s) 96, 162, 373
AsuC2I CCSGG 2 cut(s) 78, 94
AsuHPI GGTGA 3 cut(s) 17, 82, 331
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 2 cut(s) 96, 162
AvrII CCTAGG 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 113
BbsI GAAGAC 2 cut(s) 229, 329
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 4 cut(s) 141, 186, 234, 258
BceAI ACGGC 2 cut(s) 228, 324
BciT130I CCWGG 2 cut(s) 73, 289
BcnI CCSGG 2 cut(s) 78, 94
BfaI CTAG 3 cut(s) 53, 243, 415
BfoI RGCGCY 1 cut(s) 272
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 130, 200, 248, 267, 272, 360
BlnI CCTAGG 1 cut(s) 242
BlsI GCNGC 6 cut(s) 131, 201, 249, 268, 273, 361
Bme1390I CCNGG 4 cut(s) 73, 78, 94, 289
Bme18I GGWCC 2 cut(s) 96, 162
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 96, 162, 373
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 4 cut(s) 73, 78, 94, 289
BmrI ACTGGG 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 133
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 2 cut(s) 229, 329
BpmI CTGGAG 1 cut(s) 271
BpuMI CCSGG 2 cut(s) 78, 94
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 150, 242
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 177, 307
Bse118I RCCGGY 2 cut(s) 101, 105
Bse1I ACTGG 1 cut(s) 128
BseBI CCWGG 2 cut(s) 73, 289
BseDI CCNNGG 2 cut(s) 150, 242
BseLI CCNNNNNNNGG 3 cut(s) 105, 177, 307
BseMII CTCAG 2 cut(s) 11, 401
BseNI ACTGG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 4 cut(s) 141, 186, 234, 258
BseYI CCCAGC 1 cut(s) 375
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 76, 105, 374, 392
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 327
BsiSI CCGG 6 cut(s) 77, 94, 102, 106, 116, 133
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 3 cut(s) 105, 177, 307
BsmFI GGGAC 1 cut(s) 349
BsnI GGCC 4 cut(s) 76, 105, 374, 392
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 3 cut(s) 185, 190, 406
BspACI CCGC 3 cut(s) 63, 266, 359
BspANI GGCC 4 cut(s) 76, 105, 374, 392
BspCNI CTCAG 2 cut(s) 12, 400
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 1 cut(s) 180
BsrFI RCCGGY 2 cut(s) 101, 105
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 2 cut(s) 101, 105
BssECI CCNNGG 2 cut(s) 150, 242
BssMI GATC 3 cut(s) 185, 190, 406
BssNI GRCGYC 1 cut(s) 362
BssT1I CCWWGG 2 cut(s) 150, 242
Bst2UI CCWGG 2 cut(s) 73, 289
Bst4CI ACNGT 2 cut(s) 145, 180
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 258, 262
BstDEI CTNAG 2 cut(s) 20, 387
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 2 cut(s) 266, 271
BstKTI GATC 3 cut(s) 188, 193, 409
BstMBI GATC 3 cut(s) 185, 190, 406
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 62, 253, 266, 398
BstNI CCWGG 2 cut(s) 73, 289
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 4 cut(s) 71, 76, 92, 287
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 141, 186, 234, 258
BstV2I GAAGAC 2 cut(s) 229, 329
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 4 cut(s) 76, 105, 374, 392
Cac8I GCNNGC 2 cut(s) 258, 262
CfoI GCGC 2 cut(s) 266, 271
Cfr10I RCCGGY 2 cut(s) 101, 105
Cfr13I GGNCC 3 cut(s) 96, 162, 373
CseI GACGC 2 cut(s) 205, 351
Csp6I GTAC 3 cut(s) 181, 283, 304
CviQI GTAC 3 cut(s) 181, 283, 304
DdeI CTNAG 2 cut(s) 20, 387
DpnI GATC 3 cut(s) 187, 192, 408
DpnII GATC 3 cut(s) 185, 190, 406
DraIII CACNNNGTG 1 cut(s) 312
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 150, 242
Eco47I GGWCC 2 cut(s) 96, 162
Eco52I CGGCCG 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 327
EcoRII CCWGG 2 cut(s) 71, 287
EcoT14I CCWWGG 2 cut(s) 150, 242
ErhI CCWWGG 2 cut(s) 150, 242
FaiI YATR 1 cut(s) 335
FaqI GGGAC 1 cut(s) 349
Fnu4HI GCNGC 6 cut(s) 130, 200, 248, 267, 272, 360
Fsp4HI GCNGC 6 cut(s) 130, 200, 248, 267, 272, 360
FspBI CTAG 3 cut(s) 53, 243, 415
GlaI GCGC 2 cut(s) 265, 270
GluI GCNGC 6 cut(s) 130, 200, 248, 267, 272, 360
GsaI CCCAGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 271
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 4 cut(s) 76, 105, 374, 392
HapII CCGG 6 cut(s) 77, 94, 102, 106, 116, 133
HgaI GACGC 2 cut(s) 205, 351
HhaI GCGC 2 cut(s) 266, 271
Hin1I GRCGYC 1 cut(s) 362
Hin6I GCGC 2 cut(s) 264, 269
HinP1I GCGC 2 cut(s) 264, 269
HinfI GANTC 4 cut(s) 4, 22, 297, 325
HpaII CCGG 6 cut(s) 77, 94, 102, 106, 116, 133
HphI GGTGA 3 cut(s) 17, 82, 331
Hpy166II GTNNAC 2 cut(s) 111, 306
Hpy188I TCNGA 3 cut(s) 190, 195, 352
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 111, 306
Hpy99I CGWCG 4 cut(s) 145, 214, 227, 230
HpyAV CCTTC 2 cut(s) 41, 286
HpyCH4III ACNGT 2 cut(s) 145, 180
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 111, 412
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 253, 266, 398
HpyF3I CTNAG 2 cut(s) 20, 387
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 362
HspAI GCGC 2 cut(s) 264, 269
Kzo9I GATC 3 cut(s) 185, 190, 406
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 4 cut(s) 141, 186, 234, 258
MaeI CTAG 3 cut(s) 53, 243, 415
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 187, 192, 408
MboI GATC 3 cut(s) 185, 190, 406
MboII GAAGA 9 cut(s) 26, 29, 32, 35, 38, 41, 79, 229, 334
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 113
MlyI GAGTC 2 cut(s) 31, 319
MnlI CCTC 4 cut(s) 38, 310, 348, 391
MroXI GAANNNNTTC 2 cut(s) 236, 296
MspI CCGG 6 cut(s) 77, 94, 102, 106, 116, 133
MspR9I CCNGG 4 cut(s) 73, 78, 94, 289
MteI GCGCNGCGC 1 cut(s) 267
MvaI CCWGG 2 cut(s) 73, 289
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 62, 253, 266, 398
NciI CCSGG 2 cut(s) 78, 94
NdeII GATC 3 cut(s) 185, 190, 406
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdmI GAANNNNTTC 2 cut(s) 236, 296
PfeI GAWTC 1 cut(s) 297
PflFI GACNNNGTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 6 cut(s) 131, 201, 249, 268, 273, 361
PleI GAGTC 2 cut(s) 30, 319
PpsI GAGTC 2 cut(s) 30, 319
PshAI GACNNNNGTC 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 71, 287
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 2 cut(s) 71, 287
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 3 cut(s) 96, 162, 373
PsuI RGATCY 1 cut(s) 185
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 3 cut(s) 182, 284, 305
RsaNI GTAC 3 cut(s) 181, 283, 304
SatI GCNGC 6 cut(s) 130, 200, 248, 267, 272, 360
Sau3AI GATC 3 cut(s) 185, 190, 406
Sau96I GGNCC 3 cut(s) 96, 162, 373
SchI GAGTC 2 cut(s) 31, 319
ScrFI CCNGG 4 cut(s) 73, 78, 94, 289
SduI GDGCHC 1 cut(s) 113
SetI ASST 6 cut(s) 30, 58, 156, 237, 321, 381
SinI GGWCC 2 cut(s) 96, 162
SsiI CCGC 3 cut(s) 63, 266, 359
SspMI CTAG 3 cut(s) 53, 243, 415
StyD4I CCNGG 4 cut(s) 71, 76, 92, 287
StyI CCWWGG 2 cut(s) 150, 242
TaaI ACNGT 2 cut(s) 145, 180
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 140
TauI GCSGC 2 cut(s) 269, 362
TfiI GAWTC 1 cut(s) 297
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 4 cut(s) 129, 199, 247, 271
Tsp45I GTSAC 1 cut(s) 23
Tth111I GACNNNGTC 1 cut(s) 145
VneI GTGCAC 1 cut(s) 109
VpaK11BI GGWCC 2 cut(s) 96, 162
XmaJI CCTAGG 1 cut(s) 242
XmnI GAANNNNTTC 2 cut(s) 236, 296
XspI CTAG 3 cut(s) 53, 243, 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.