MD05G1197000.v1.1

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014249
Physical Location & Seq
Forward (+)
32438573 .. 32440020
1448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1197000.v1.1.491

Sequence Viewer

Length: 465 bp
ATGAAGCAAATATTAGTTCAACTGAGTCAACTCGCTCTTCTTCTTCTTCTGCTACTTGTTGCCTGCTCTGTTCCCACAACGACGGCCCGAAACCAACGGCCAGGATTTCTCTTCACAAGAACAACAGGAAGATGCACTCCTCAGTATTGGGGGAGCAGGAGGGAGTCGTGGCCGAAGATGGTCCCACAAACATCGACGGTGTCAAAGGTGTTTGGATCAAGAGCATACGAACGTTACAGATCCGATCTGACATTGCTTGAAGCGACGGTCAGGAATGACGAGGAGAATGCTTACCCTAGGCTGGTGAAGCAGGCGAGTGCAGCACTGCTGAACTCTTATGCAAGAAAAGGGTATCCTTACACAGCATGGGAGGTCAAGACTTTGTTGATCCAAGCTTTGGTGTCTGAGAAAGCAGCTGCCCTTCAAGCCCAACACTTTTCTACTGCCAATGAGGCTTGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.34

Weight (kDa)

9.79

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 397
AclI AACGTT 1 cut(s) 232
AclWI GGATC 3 cut(s) 223, 234, 382
AcoI YGGCCR 2 cut(s) 98, 170
AfiI CCNNNNNNNGG 2 cut(s) 301, 397
AgsI TTSAA 3 cut(s) 20, 260, 425
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 2 cut(s) 395, 416
AluI AGCT 2 cut(s) 395, 416
AlwI GGATC 3 cut(s) 223, 234, 382
AoxI GGCC 3 cut(s) 84, 98, 170
ApeKI GCWGC 3 cut(s) 320, 413, 416
AspA2I CCTAGG 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 85, 181
AsuHPI GGTGA 1 cut(s) 316
AvaII GGWCC 1 cut(s) 181
AvrII CCTAGG 1 cut(s) 296
BbvI GCAGC 3 cut(s) 332, 403, 425
BccI CCATC 1 cut(s) 172
BceAI ACGGC 2 cut(s) 99, 113
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 363
BfaI CTAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 363
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 3 cut(s) 321, 414, 417
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 3 cut(s) 322, 415, 418
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 85, 181
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 296
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 397
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 296
BseLI CCNNNNNNNGG 2 cut(s) 301, 397
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 3 cut(s) 14, 155, 396
BseRI GAGGAG 2 cut(s) 129, 296
BseXI GCAGC 3 cut(s) 332, 403, 425
BsgI GTGCAG 1 cut(s) 339
BshFI GGCC 3 cut(s) 86, 100, 172
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 301, 397
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 3 cut(s) 86, 100, 172
Bsp143I GATC 4 cut(s) 215, 239, 244, 387
BspANI GGCC 3 cut(s) 86, 100, 172
BspCNI CTCAG 3 cut(s) 15, 154, 397
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 3 cut(s) 223, 234, 382
BspQI GCTCTTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 4 cut(s) 215, 239, 244, 387
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 199, 268
Bst6I CTCTTC 2 cut(s) 42, 116
BstC8I GCNNGC 2 cut(s) 64, 312
BstDEI CTNAG 3 cut(s) 23, 141, 405
BstKTI GATC 4 cut(s) 218, 242, 247, 390
BstMBI GATC 4 cut(s) 215, 239, 244, 387
BstMWI GCNNNNNNNGC 4 cut(s) 307, 320, 425, 452
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 332, 403, 425
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 3 cut(s) 86, 100, 172
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 64, 312
Cfr13I GGNCC 2 cut(s) 85, 181
CviAII CATG 1 cut(s) 366
CviJI RGCY 8 cut(s) 86, 100, 172, 301, 395, 416, 428, 455
CviKI_1 RGCY 8 cut(s) 86, 100, 172, 301, 395, 416, 428, 455
DdeI CTNAG 3 cut(s) 23, 141, 405
DpnI GATC 4 cut(s) 217, 241, 246, 389
DpnII GATC 4 cut(s) 215, 239, 244, 387
EaeI YGGCCR 2 cut(s) 98, 170
Eam1104I CTCTTC 2 cut(s) 42, 116
EarI CTCTTC 2 cut(s) 42, 116
Eco130I CCWWGG 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 1 cut(s) 369
FaiI YATR 3 cut(s) 226, 339, 367
FaqI GGGAC 1 cut(s) 167
FatI CATG 1 cut(s) 365
Fnu4HI GCNGC 3 cut(s) 321, 414, 417
Fsp4HI GCNGC 3 cut(s) 321, 414, 417
FspBI CTAG 1 cut(s) 297
GluI GCNGC 3 cut(s) 321, 414, 417
HaeIII GGCC 3 cut(s) 86, 100, 172
Hin1II CATG 1 cut(s) 369
HincII GTYRAC 1 cut(s) 29
HindII GTYRAC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 2 cut(s) 25, 164
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 3 cut(s) 244, 249, 406
Hpy188III TCNNGA 3 cut(s) 219, 271, 376
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 3 cut(s) 85, 199, 268
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 2 cut(s) 199, 268
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 3 cut(s) 135, 320, 341
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 320, 425, 452
HpyF3I CTNAG 3 cut(s) 23, 141, 405
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 1 cut(s) 369
Kzo9I GATC 4 cut(s) 215, 239, 244, 387
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 153
LpnPI CCDG 8 cut(s) 76, 87, 111, 114, 142, 256, 287, 296
Lsp1109I GCAGC 3 cut(s) 332, 403, 425
LweI GCATC 1 cut(s) 122
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 2 cut(s) 233, 458
MalI GATC 4 cut(s) 217, 241, 246, 389
MboI GATC 4 cut(s) 215, 239, 244, 387
MboII GAAGA 7 cut(s) 29, 32, 35, 38, 103, 141, 187
MflI RGATCY 1 cut(s) 239
MlyI GAGTC 2 cut(s) 34, 173
MnlI CCTC 5 cut(s) 150, 153, 274, 364, 445
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 4 cut(s) 307, 320, 425, 452
NdeII GATC 4 cut(s) 215, 239, 244, 387
NlaIII CATG 1 cut(s) 369
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 458
PciSI GCTCTTC 1 cut(s) 42
PctI GAATGC 1 cut(s) 292
PflFI GACNNNGTC 1 cut(s) 199
PflMI CCANNNNNTGG 1 cut(s) 397
PkrI GCNGC 3 cut(s) 322, 415, 418
PleI GAGTC 2 cut(s) 33, 172
PpsI GAGTC 2 cut(s) 33, 172
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 2 cut(s) 85, 181
PsuI RGATCY 1 cut(s) 239
PsyI GACNNNGTC 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 42
SatI GCNGC 3 cut(s) 321, 414, 417
Sau3AI GATC 4 cut(s) 215, 239, 244, 387
Sau96I GGNCC 2 cut(s) 85, 181
SchI GAGTC 2 cut(s) 34, 173
ScrFI CCNGG 1 cut(s) 102
SetI ASST 5 cut(s) 210, 235, 375, 397, 418
SfaNI GCATC 1 cut(s) 122
SinI GGWCC 1 cut(s) 181
SspI AATATT 1 cut(s) 12
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 199, 268
TaiI ACGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 194
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 3 cut(s) 320, 413, 416
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 330
Tth111I GACNNNGTC 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 181
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.