Prupe.8G223500_v2.0.a1

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014249
Physical Location & Seq
Forward (+)
20189766 .. 20190797
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G223500.1

Sequence Viewer

Length: 456 bp
ATGTTGAGGCCTTCTCAACTGAGTCAACTCGTTCTTCTTCTTCTTCTTATTGCTTGCTCTGTACCCTCAACAACAGCCAAAACCCAAAGGCCAGGCTTTCTCTTCACAAGAACCACAGGAAGATGCACTCCACACTATTGGGGGAGCAGGAGGGAGGCTTGGCCGAGGATGGTCCCACAGACATCAACGGTGTCGAAGGTGTTCGGATCAAGAGCCTACGAACGGTACAGATCTGATCTGACGCTACTGGAAGCGACGGTCAGGAACGACGAGGAGAACGTCTACCCTAGGCTGCTGAAGCAGGCGAGCGCCGCCCTCCTGAACTCTTACGCCAGGAAGGGGTTTCCGTACAGTGCTTGGGAGGTGAAGACTTTGGTCATTCAAGCTCTGGTGTCGGATAAGGCTGCAGCCCTTCAGGCCCAGCGCTTCTCCGTTGCCAATGAGGCTTGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.99

Weight (kDa)

10.02

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 282
AciI CCGC 1 cut(s) 312
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 317, 398
AfaI GTAC 3 cut(s) 63, 227, 350
AfeI AGCGCT 1 cut(s) 425
AfiI CCNNNNNNNGG 2 cut(s) 222, 339
AgsI TTSAA 1 cut(s) 383
AjnI CCWGG 2 cut(s) 91, 332
AluBI AGCT 1 cut(s) 386
AluI AGCT 1 cut(s) 386
AlwI GGATC 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 425
AoxI GGCC 4 cut(s) 8, 89, 161, 417
ApeKI GCWGC 3 cut(s) 292, 404, 407
Asp700I GAANNNNTTC 1 cut(s) 200
AspA2I CCTAGG 1 cut(s) 287
AspLEI GCGC 2 cut(s) 311, 426
AspS9I GGNCC 2 cut(s) 172, 418
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 172
AvrII CCTAGG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 279, 391, 419
BccI CCATC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 93, 334
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 2 cut(s) 312, 427
BglI GCCNNNNNGGC 2 cut(s) 416, 443
BglII AGATCT 1 cut(s) 230
BisI GCNGC 4 cut(s) 293, 312, 405, 408
BlnI CCTAGG 1 cut(s) 287
BlsI GCNGC 4 cut(s) 294, 313, 406, 409
Bme1390I CCNGG 2 cut(s) 93, 334
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 172, 418
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 93, 334
BmsI GCATC 1 cut(s) 113
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 374
BplI GAGNNNNNCTC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 164, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 339
Bse1I ACTGG 1 cut(s) 252
BseBI CCWGG 2 cut(s) 93, 334
BseDI CCNNGG 2 cut(s) 164, 287
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 2 cut(s) 222, 339
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 3 cut(s) 279, 391, 419
BseYI CCCAGC 1 cut(s) 420
BshFI GGCC 4 cut(s) 10, 91, 163, 419
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 222, 339
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 4 cut(s) 10, 91, 163, 419
Bsp143I GATC 3 cut(s) 206, 230, 235
BspACI CCGC 1 cut(s) 312
BspANI GGCC 4 cut(s) 10, 91, 163, 419
BspCNI CTCAG 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 1 cut(s) 214
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 164, 287
BssMI GATC 3 cut(s) 206, 230, 235
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 93, 334
Bst4CI ACNGT 4 cut(s) 190, 225, 259, 353
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 4 cut(s) 55, 303, 307, 448
BstDEI CTNAG 1 cut(s) 20
BstF5I GGATG 1 cut(s) 174
BstH2I RGCGCY 2 cut(s) 312, 427
BstHHI GCGC 2 cut(s) 311, 426
BstKTI GATC 3 cut(s) 209, 233, 238
BstMBI GATC 3 cut(s) 206, 230, 235
BstMWI GCNNNNNNNGC 4 cut(s) 298, 311, 416, 443
BstNI CCWGG 2 cut(s) 93, 334
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 91, 332
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 3 cut(s) 279, 391, 419
BstV2I GAAGAC 1 cut(s) 374
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 4 cut(s) 10, 91, 163, 419
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 358
Cac8I GCNNGC 4 cut(s) 55, 303, 307, 448
CfoI GCGC 2 cut(s) 311, 426
Cfr13I GGNCC 2 cut(s) 172, 418
CseI GACGC 1 cut(s) 250
Csp6I GTAC 3 cut(s) 62, 226, 349
CviQI GTAC 3 cut(s) 62, 226, 349
DdeI CTNAG 1 cut(s) 20
DpnI GATC 3 cut(s) 208, 232, 237
DpnII GATC 3 cut(s) 206, 230, 235
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 287
Eco147I AGGCCT 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 172
Eco47III AGCGCT 1 cut(s) 425
Eco57I CTGAAG 2 cut(s) 317, 398
EcoRII CCWGG 2 cut(s) 91, 332
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaqI GGGAC 1 cut(s) 158
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 293, 312, 405, 408
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 293, 312, 405, 408
FspBI CTAG 1 cut(s) 288
GlaI GCGC 2 cut(s) 310, 425
GluI GCNGC 4 cut(s) 293, 312, 405, 408
GsaI CCCAGC 1 cut(s) 424
HaeII RGCGCY 2 cut(s) 312, 427
HaeIII GGCC 4 cut(s) 10, 91, 163, 419
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 311, 426
Hin6I GCGC 2 cut(s) 309, 424
HinP1I GCGC 2 cut(s) 309, 424
HincII GTYRAC 1 cut(s) 26
HindII GTYRAC 1 cut(s) 26
HinfI GANTC 1 cut(s) 22
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 2 cut(s) 26, 283
Hpy188I TCNGA 4 cut(s) 206, 235, 240, 397
Hpy188III TCNNGA 3 cut(s) 210, 262, 319
Hpy8I GTNNAC 2 cut(s) 26, 283
Hpy99I CGWCG 2 cut(s) 259, 272
HpyAV CCTTC 4 cut(s) 21, 190, 331, 422
HpyCH4III ACNGT 4 cut(s) 190, 225, 259, 353
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 126, 407
HpyF10VI GCNNNNNNNGC 4 cut(s) 298, 311, 416, 443
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 1 cut(s) 279
HspAI GCGC 2 cut(s) 309, 424
Kzo9I GATC 3 cut(s) 206, 230, 235
LmnI GCTCC 1 cut(s) 144
Lsp1109I GCAGC 3 cut(s) 279, 391, 419
LweI GCATC 1 cut(s) 113
MaeI CTAG 1 cut(s) 288
MaeII ACGT 1 cut(s) 279
MalI GATC 3 cut(s) 208, 232, 237
MboI GATC 3 cut(s) 206, 230, 235
MboII GAAGA 7 cut(s) 26, 29, 32, 35, 94, 132, 379
MflI RGATCY 1 cut(s) 230
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 8 cut(s) 76, 144, 148, 159, 265, 326, 355, 436
MroXI GAANNNNTTC 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 93, 334
MvaI CCWGG 2 cut(s) 93, 334
MwoI GCNNNNNNNGC 4 cut(s) 298, 311, 416, 443
NdeII GATC 3 cut(s) 206, 230, 235
NlaIV GGNNCC 1 cut(s) 174
NmeAIII GCCGAG 1 cut(s) 189
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 200
PkrI GCNGC 4 cut(s) 294, 313, 406, 409
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 374
Psp6I CCWGG 2 cut(s) 91, 332
PspFI CCCAGC 1 cut(s) 420
PspGI CCWGG 2 cut(s) 91, 332
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 172, 418
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 3 cut(s) 63, 227, 350
RsaNI GTAC 3 cut(s) 62, 226, 349
SatI GCNGC 4 cut(s) 293, 312, 405, 408
Sau3AI GATC 3 cut(s) 206, 230, 235
Sau96I GGNCC 2 cut(s) 172, 418
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 93, 334
SetI ASST 4 cut(s) 201, 282, 366, 388
SfaNI GCATC 1 cut(s) 113
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 172
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 1 cut(s) 312
SspMI CTAG 1 cut(s) 288
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 91, 332
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 4 cut(s) 190, 225, 259, 353
TaiI ACGT 1 cut(s) 282
TaqI TCGA 1 cut(s) 194
TauI GCSGC 1 cut(s) 314
TscAI CASTG 1 cut(s) 358
TseI GCWGC 3 cut(s) 292, 404, 407
TspGWI ACGGA 2 cut(s) 336, 421
TspRI CASTG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 172
XmaJI CCTAGG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.