Rroxscaffold_7G00180520

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014249
Physical Location & Seq
Reverse (-)
19882066 .. 19882506
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180520.1

Sequence Viewer

Length: 441 bp
ATGACTCGTCCTTTTCAACTGAGTCACCTCGTTCTTCTTCTTCTTCTAGCTACTACCGCAACAGCCAGGCCGGGCTTTCTCTTCACTCGGACCACCGGCCGGTGCACACCGGACTACTGGGGCAGCCGGGTGGAGTCGTGGCCGAGGATGGTCCCGCAGACATCGACGGTGTCCAAGGTGTTCGGGTCCAGAGCCTACGAACGGTACAGATCCGATCTGACGCTGCTGGAAGCGACGGCAAGGAGCGACGACGAGAACGTCTTCCCTAGGCTGCTGAAGCAGGCGAGCGCGGCGCTGCTCAACTCCTACTCCAGGAAGGGGTTCCCGTACACGGCGTGGGAGGTGAAGACTCGGGTCATACAGGGCTTGGTGTCCGAGAGGGCGGCATCCCGTCAGGCCCAGCTATTCTCTCAGGCCAATGAGGCTTGTGATCTACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.5

Weight (kDa)

9.92

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20515
fragaria_vesca FvH4_2g21450
malus_domestica MD05G1197000.v1.1
prunus_persica Prupe.8G223500_v2.0.a1
pyrus_communis pycom10g15900
rosa_chinensis RchiOBHm_Chr6g0288091
rosa_laevigata RLG00000012440
rosa_multiflora Rmu_co7961866.1_g000001 Rmu_sc0001117.1_g000002 Rmu_sc0010766.1_g000001
rosa_roxburghii Rroxscaffold_7G00180520
rosa_rugosa Rorug06G0194200
rosa_samantha Rh6AG304300 Rh6BG310300 Rh6CG318300 Rh6DG303200
rosa_wichuraiana Rw6G026330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AccII CGCG 1 cut(s) 290
AciI CCGC 4 cut(s) 57, 155, 290, 383
AclWI GGATC 1 cut(s) 204
AcoI YGGCCR 2 cut(s) 97, 140
AcuI CTGAAG 1 cut(s) 296
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 2 cut(s) 206, 329
AfiI CCNNNNNNNGG 5 cut(s) 99, 201, 312, 318, 331
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 2 cut(s) 65, 311
AluBI AGCT 2 cut(s) 50, 403
AluI AGCT 2 cut(s) 50, 403
Alw21I GWGCWC 1 cut(s) 107
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 5 cut(s) 68, 97, 140, 396, 414
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 4 cut(s) 123, 223, 271, 295
Asp700I GAANNNNTTC 2 cut(s) 260, 320
AspA2I CCTAGG 1 cut(s) 266
AspLEI GCGC 2 cut(s) 290, 295
AspS9I GGNCC 4 cut(s) 90, 151, 186, 397
AsuC2I CCSGG 2 cut(s) 72, 128
AsuHPI GGTGA 2 cut(s) 17, 355
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 3 cut(s) 90, 151, 186
AvrII CCTAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 107
BbsI GAAGAC 2 cut(s) 253, 353
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 4 cut(s) 135, 210, 258, 282
BccI CCATC 1 cut(s) 142
BceAI ACGGC 2 cut(s) 252, 348
BciT130I CCWGG 2 cut(s) 67, 313
BcnI CCSGG 2 cut(s) 72, 128
BfaI CTAG 3 cut(s) 47, 267, 439
BfoI RGCGCY 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 6 cut(s) 124, 224, 272, 291, 296, 384
BlnI CCTAGG 1 cut(s) 266
BlsI GCNGC 6 cut(s) 125, 225, 273, 292, 297, 385
Bme1390I CCNGG 4 cut(s) 67, 72, 128, 313
Bme18I GGWCC 3 cut(s) 90, 151, 186
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 4 cut(s) 90, 151, 186, 397
BmiI GGNNCC 3 cut(s) 153, 187, 323
BmrFI CCNGG 4 cut(s) 67, 72, 128, 313
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 395
BmuI ACTGGG 1 cut(s) 127
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 2 cut(s) 253, 353
BpmI CTGGAG 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 72, 128
BsaJI CCNNGG 3 cut(s) 143, 174, 266
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 5 cut(s) 99, 201, 312, 318, 331
Bse118I RCCGGY 2 cut(s) 95, 99
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 2 cut(s) 67, 313
BseDI CCNNGG 3 cut(s) 143, 174, 266
BseGI GGATG 2 cut(s) 153, 386
BseLI CCNNNNNNNGG 5 cut(s) 99, 201, 312, 318, 331
BseMII CTCAG 2 cut(s) 11, 425
BseNI ACTGG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 107
BseX3I CGGCCG 1 cut(s) 97
BseXI GCAGC 4 cut(s) 135, 210, 258, 282
BseYI CCCAGC 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 290
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 5 cut(s) 70, 99, 142, 398, 416
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 5 cut(s) 71, 96, 100, 110, 127
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 5 cut(s) 99, 201, 312, 318, 331
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 5 cut(s) 70, 99, 142, 398, 416
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 3 cut(s) 209, 214, 430
BspACI CCGC 4 cut(s) 57, 155, 290, 383
BspANI GGCC 5 cut(s) 70, 99, 142, 398, 416
BspCNI CTCAG 2 cut(s) 12, 424
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 3 cut(s) 153, 187, 323
BspPI GGATC 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 95, 99
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 2 cut(s) 95, 99
BssECI CCNNGG 3 cut(s) 143, 174, 266
BssMI GATC 3 cut(s) 209, 214, 430
BssT1I CCWWGG 2 cut(s) 174, 266
Bst2UI CCWGG 2 cut(s) 67, 313
Bst4CI ACNGT 2 cut(s) 169, 204
Bst6I CTCTTC 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 282, 286
BstDEI CTNAG 2 cut(s) 20, 411
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 2 cut(s) 153, 386
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 2 cut(s) 290, 295
BstKTI GATC 3 cut(s) 212, 217, 433
BstMBI GATC 3 cut(s) 209, 214, 430
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 4 cut(s) 56, 277, 290, 422
BstNI CCWGG 2 cut(s) 67, 313
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 4 cut(s) 65, 70, 126, 311
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 4 cut(s) 135, 210, 258, 282
BstV2I GAAGAC 2 cut(s) 253, 353
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BstZI CGGCCG 1 cut(s) 97
BsuRI GGCC 5 cut(s) 70, 99, 142, 398, 416
BtsCI GGATG 2 cut(s) 153, 386
Cac8I GCNNGC 2 cut(s) 282, 286
CfoI GCGC 2 cut(s) 290, 295
Cfr10I RCCGGY 2 cut(s) 95, 99
Cfr13I GGNCC 4 cut(s) 90, 151, 186, 397
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 205, 328
CviQI GTAC 2 cut(s) 205, 328
DdeI CTNAG 2 cut(s) 20, 411
DpnI GATC 3 cut(s) 211, 216, 432
DpnII GATC 3 cut(s) 209, 214, 430
DraIII CACNNNGTG 1 cut(s) 336
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 2 cut(s) 97, 140
EagI CGGCCG 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EclXI CGGCCG 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 174, 266
Eco47I GGWCC 3 cut(s) 90, 151, 186
Eco52I CGGCCG 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 65, 311
EcoT14I CCWWGG 2 cut(s) 174, 266
ErhI CCWWGG 2 cut(s) 174, 266
FaiI YATR 1 cut(s) 359
FaqI GGGAC 1 cut(s) 137
FauI CCCGC 1 cut(s) 162
Fnu4HI GCNGC 6 cut(s) 124, 224, 272, 291, 296, 384
FokI GGATG 2 cut(s) 160, 373
Fsp4HI GCNGC 6 cut(s) 124, 224, 272, 291, 296, 384
FspBI CTAG 3 cut(s) 47, 267, 439
GlaI GCGC 2 cut(s) 289, 294
GluI GCNGC 6 cut(s) 124, 224, 272, 291, 296, 384
GsaI CCCAGC 1 cut(s) 403
GsuI CTGGAG 1 cut(s) 295
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 5 cut(s) 70, 99, 142, 398, 416
HapII CCGG 5 cut(s) 71, 96, 100, 110, 127
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 290, 295
Hin6I GCGC 2 cut(s) 288, 293
HinP1I GCGC 2 cut(s) 288, 293
HinfI GANTC 4 cut(s) 4, 22, 134, 349
HpaII CCGG 5 cut(s) 71, 96, 100, 110, 127
HphI GGTGA 2 cut(s) 17, 355
Hpy166II GTNNAC 2 cut(s) 105, 330
Hpy188I TCNGA 4 cut(s) 90, 214, 219, 376
Hpy188III TCNNGA 1 cut(s) 189
Hpy8I GTNNAC 2 cut(s) 105, 330
Hpy99I CGWCG 4 cut(s) 169, 238, 251, 254
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 2 cut(s) 169, 204
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 277, 290, 422
HpyF3I CTNAG 2 cut(s) 20, 411
HpySE526I ACGT 1 cut(s) 258
HspAI GCGC 2 cut(s) 288, 293
Kzo9I GATC 3 cut(s) 209, 214, 430
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 4 cut(s) 135, 210, 258, 282
LweI GCATC 1 cut(s) 395
MaeI CTAG 3 cut(s) 47, 267, 439
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 211, 216, 432
MboI GATC 3 cut(s) 209, 214, 430
MboII GAAGA 7 cut(s) 26, 29, 32, 35, 73, 253, 358
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 107
MlyI GAGTC 3 cut(s) 31, 143, 343
MnlI CCTC 5 cut(s) 38, 138, 334, 372, 415
MroXI GAANNNNTTC 2 cut(s) 260, 320
MspI CCGG 5 cut(s) 71, 96, 100, 110, 127
MspR9I CCNGG 4 cut(s) 67, 72, 128, 313
MteI GCGCNGCGC 1 cut(s) 291
MvaI CCWGG 2 cut(s) 67, 313
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 4 cut(s) 56, 277, 290, 422
NciI CCSGG 2 cut(s) 72, 128
NdeII GATC 3 cut(s) 209, 214, 430
NlaIV GGNNCC 3 cut(s) 153, 187, 323
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 2 cut(s) 260, 320
PflFI GACNNNGTC 1 cut(s) 169
PfoI TCCNGGA 1 cut(s) 311
PkrI GCNGC 6 cut(s) 125, 225, 273, 292, 297, 385
PleI GAGTC 3 cut(s) 30, 142, 343
PpsI GAGTC 3 cut(s) 30, 142, 343
PshAI GACNNNNGTC 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 65, 311
PspFI CCCAGC 1 cut(s) 399
PspGI CCWGG 2 cut(s) 65, 311
PspN4I GGNNCC 3 cut(s) 153, 187, 323
PspPI GGNCC 4 cut(s) 90, 151, 186, 397
PsuI RGATCY 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 169
RsaI GTAC 2 cut(s) 206, 329
RsaNI GTAC 2 cut(s) 205, 328
SatI GCNGC 6 cut(s) 124, 224, 272, 291, 296, 384
Sau3AI GATC 3 cut(s) 209, 214, 430
Sau96I GGNCC 4 cut(s) 90, 151, 186, 397
SchI GAGTC 3 cut(s) 31, 143, 343
ScrFI CCNGG 4 cut(s) 67, 72, 128, 313
SduI GDGCHC 1 cut(s) 107
SetI ASST 6 cut(s) 30, 52, 180, 261, 345, 405
SfaNI GCATC 1 cut(s) 395
SinI GGWCC 3 cut(s) 90, 151, 186
SsiI CCGC 4 cut(s) 57, 155, 290, 383
SspMI CTAG 3 cut(s) 47, 267, 439
StyD4I CCNGG 4 cut(s) 65, 70, 126, 311
StyI CCWWGG 2 cut(s) 174, 266
TaaI ACNGT 2 cut(s) 169, 204
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 164
TauI GCSGC 2 cut(s) 293, 386
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 4 cut(s) 123, 223, 271, 295
Tsp45I GTSAC 1 cut(s) 23
Tth111I GACNNNGTC 1 cut(s) 169
VneI GTGCAC 1 cut(s) 103
VpaK11BI GGWCC 3 cut(s) 90, 151, 186
XagI CCTNNNNNAGG 1 cut(s) 310
XmaJI CCTAGG 1 cut(s) 266
XmnI GAANNNNTTC 2 cut(s) 260, 320
XspI CTAG 3 cut(s) 47, 267, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.