Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010697

Physical Location & Seq

Genomic Coordinates Forward (+)

8858962 .. 8864865

Gene Structure 5904 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G20580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGGCTCCAACTCAGGATCCCAACAGTGTCGGAGGCGGTGCGAAGAAAGATGAAGCTACCTTGAAGGTTCCGTCTAAGGATCCCAAGAAGAAGGACGAGAAAAAGGATGAGGATTTGTCTGAAGAGGACTTGGAACTAAAGCAGAACCTTGAGCTCTATGTTGAGAGGGTTCAGGATCCTAATCCGGAATTGCAGAAGGCTGCCCTTGAGAGCATGAGGCAGGAAATCCGAGCTTCAACAAGTTCCATGACTTCAGTTCCCAAACCACTAAAGTTTCTGCGTCCCCATTATGGAACTCTTAAAGCGTTTCATGAAACAATGGCTGATTCTGATCTCAAAAAGTACCTGTCCGATATCCTGTCTGTCCTGGCCCTTACCATGTCTGCAGATGGTGAAAGGGAAAGCTTAAGGTTTAGATTGATTGGAACAGAGGGTGACATTGGATCGTGGGGTCATGAATATGTCAGGAATTTAGCTGGAGAGATTGCACAAGAGTATACAAAGCGTCAGAGTGAGGAGGCCTCTATTGACGATTTAATGGAGCTTGTGCAACAAATTGTTGCTTTTCACATGAAGCATAATGCAGAAACCGAAGCTGTTGATCTTTTAATGGATGTTGAGGATCTTGATCTCTTACTTGAGCATGTAGACAAAACAAATTTCAAGAGGACATGCAACTATCTCACAAGTGCAGCTAGGTACCTTCCTGGACCAGATGACATGTTGGTTCTAGATATTTCCTACATGATTTACATGAAGTTTGAAGAGTATCCAAACGCTCTGCAGATTGCACTATTTCTTGATAACACGCAGTATGTTAAACAAGTATTTACCTCATGTACGGATCTGCTGAAGAAGAAACAGTTCTGCTACATGATTGCTCGTCATGGTATAACCTTTGAACTTGACGATGAGATGGTTGCAGATGATGATGACCGGGAAGCCCTACAGGATATTGTTAACAACACTAAGTTAAGTGAAGGATATCTGACCCTTGCAAGGGATATTGAGGTCATGGAGGCCAAAACGCCTGAAGACATCTACAAGGCTCACTTACTCGACGGCAGGGCTAGCTCCGGTGCAAGCGTGGATTCTGCTAGACAGAATTTGGCTGCTACCTTTGTCAATGCATTCGTAAATGCTGGTTTTGGTCAGGACAAACTAATGACAGTACCTTCAGACTCAACCACCGGATCTTCTGGAAACTGGCTCTTCAAAAATAAAGAACATGGCAAGACTAGTGCAGCTGCTAGTCTGGGTATGATTCAACTGTGGGATGTGGACTCTGGACTTTCCCAACTCGACAAATATTTCCATAGTAATGATAATCCTATCATTGCTGGAGCACTGCTGGGAGTTGGGATTGTAAATTGTGGCATTAAGAACGATTGTGATCCTGCATTGGCACTTCTTGGAGACTATATAGACAAAGAGGATTCATCTGTCAGAATCGGTGCTATCATGGGTCTTGGAATTTCATACGCAGGTTCCCAAAATGACCAGATTCGAAACAAATTGTCCCCGATACTGAATGATGCAAAAGCGCCTCTTGATGTGATTGCATTTGCTTCACTTTCTTTGGGAATGATATATGTTGGTTCCTGTAACGAAGAAGTAGCCCAGTCTATTATATTTGCTTTGATGGATCGAAGTGAGGCAGAACTGGGTGATGCCCTCACTCGTTTCTTGCCACTTGGTCTTGGACTTCTATACCTGGGCAAACAGGAAAGTGTAGAGGCTACAGCTGAAGTTTCGAAGACCTTCAATGAGAAGATTAGAAAGTATTGTGATATGACACTTCTTTCTTGTGCTTATGCTGGAACTGGAAATGTCCTAAAGGTACAAGACCTTCTTGCTCAGTGTGGCGAGCATCTTGAGAAAGGTGACATCCACCAGGGTCCAGCTGTGCTTGGGTTAGCAATGGTTGCTATGTCTGAAGAACTGGGAGTTGATATGGAGATCCGTTCTCTGGAGCGGATGCTACAATACGGAGAACAAAACATTCGGCGTGCTGTGCCTTTGGCCCTTGGTCTCCTTTGTATATCCAATCCAAAGGTGACTGTAATGGACACCTTGAGCCGGCTTAGCCATGACACAGATTCGGAAGTTGCCATGTCAGCAATTATATCATTGGGACTGATTGGCGCTGGAACTAATAACGCAAGGATAGCCGGGATGCTTCGGAACCTCTCCAGCTATTATTACAAAGACATGAGTCTTCTCTTCTGTGTGCGTATTGCTCAAGGGCTTGTGCACATGGGAAAGGGTCTCTTGACACTCAGCCCCTTCCACTCTGAACGGTTCTTGCTATCGCCAACCGCACTTGCTGGTATAGTGACACTTTTGCATGCATGCCTTGACATGAAACCAATCATACTCGGGAAGTACCATTATGTGCTGTACTTCCTCGTTTTGGCGATGCAACCAAGGATGATGTTGACAGTGGATGAAAACCTGAAGCCACTGTCAGTGCCAGTGCGGGTAGGACAAGCAGTTGATGTGGTTGGGCAAGCGGGTCGGCCCAAGACCATAACCGGTTTCCAGACACACTCCACACCTGTTCTCCTGGCTGCTGGAGAGAGAGCCGAACTAGCCACGGACAAATACATTCCTCTGTCGCCAATACTAGAAGGATTCATCATCTTAAAGGAGAACCCAGACTACAGAGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0001101 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010498 GO:0014070 GO:0016020 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030163 GO:0031597 GO:0032182 GO:0032991 GO:0034515 GO:0042221 GO:0042493 GO:0043130 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051726 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PC_rep

Protein Analysis

891

Amino Acids

98.14

Weight (kDa)

4.96

Isoelectric Point (pI)

36.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN1_RPN2_N	PF17781	50 - 352	1.6e-124	RPN1 N-terminal domain
RPN1_C	PF18051	835 - 888	2.9e-31	26S proteasome non-ATPase regulatory subunit RPN1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20580 AT4G28470
fragaria_vesca	FvH4_4g14560
malus_domestica	MD09G1242100.v1.1 MD17G1234500.v1.1
prunus_persica	Prupe.3G134800_v2.0.a1
pyrus_communis	pycom09g16070
rosa_chinensis	RchiOBHm_Chr4g0418261
rosa_laevigata	RLG00000007882 RLG00000014797
rosa_multiflora	Rmu_co8313613.1_g000001 Rmu_sc0000032.1_g000014
rosa_roxburghii	Rroxscaffold_5G00361020 Rroxscaffold_5G00361440
rosa_rugosa	Rorug04G0151500
rosa_samantha	Rh4BG217500 Rh4CG224800 Rh4DG211700
rosa_wichuraiana	Rw4G018100 Rw6G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1475
Acc65I	GGTACC	1 cut(s)	699
AccB1I	GGYRCC	1 cut(s)	699
AccBSI	CCGCTC	1 cut(s)	1975
AccI	GTMKAC	2 cut(s)	497, 648
AccIII	TCCGGA	1 cut(s)	184
AciI	CCGC	5 cut(s)	36, 1975, 2319, 2479, 2513
AcsI	RAATTY	4 cut(s)	469, 658, 1105, 1473
AcuI	CTGAAG	8 cut(s)	141, 237, 872, 1053, 1161, 1767, 1956, 2477
AfaI	GTAC	7 cut(s)	344, 701, 841, 1173, 1842, 2387, 2402
AfiI	CCNNNNNNNGG	6 cut(s)	290, 999, 1000, 1969, 2079, 2413
AflII	CTTAAG	1 cut(s)	406
AflIII	ACRYGT	1 cut(s)	720
AgeI	ACCGGT	1 cut(s)	2534
AgsI	TTSAA	8 cut(s)	64, 237, 664, 764, 902, 1216, 1268, 1765
AhlI	ACTAGT	1 cut(s)	1238
AjnI	CCWGG	5 cut(s)	366, 706, 1713, 1893, 2565
Alw21I	GWGCWC	3 cut(s)	156, 1348, 2256
Alw26I	GTCTC	3 cut(s)	1410, 2036, 2273
Alw44I	GTGCAC	1 cut(s)	2252
Ama87I	CYCGRG	1 cut(s)	2378
Aor13HI	TCCGGA	1 cut(s)	184
AoxI	GGCC	5 cut(s)	369, 519, 1020, 2022, 2519
ApaLI	GTGCAC	1 cut(s)	2252
ApeKI	GCWGC	6 cut(s)	200, 692, 1112, 1244, 1247, 2570
ApoI	RAATTY	4 cut(s)	469, 658, 1105, 1473
AsiGI	ACCGGT	1 cut(s)	2534
Asp700I	GAANNNNTTC	2 cut(s)	863, 1760
Asp718I	GGTACC	1 cut(s)	699
AspLEI	GCGC	2 cut(s)	1546, 2147
AspS9I	GGNCC	5 cut(s)	370, 710, 1898, 2023, 2520
AsuC2I	CCSGG	2 cut(s)	938, 2173
AsuHPI	GGTGA	5 cut(s)	404, 446, 1679, 1895, 2068
AsuII	TTCGAA	2 cut(s)	1507, 1754
AsuNHI	GCTAGC	1 cut(s)	1070
AvaI	CYCGRG	1 cut(s)	2378
AvaII	GGWCC	2 cut(s)	710, 1898
BaeGI	GKGCMC	1 cut(s)	2256
BamHI	GGATCC	3 cut(s)	16, 79, 175
BanI	GGYRCC	1 cut(s)	699
BanII	GRGCYC	1 cut(s)	156
BarI	GAAGNNNNNNTAC	2 cut(s)	1833, 1865
BbsI	GAAGAC	3 cut(s)	1041, 1763, 2210
Bbv12I	GWGCWC	3 cut(s)	156, 1348, 2256
BbvI	GCAGC	6 cut(s)	187, 704, 1099, 1234, 1256, 2557
BccI	CCATC	3 cut(s)	383, 910, 1636
BceAI	ACGGC	1 cut(s)	1078
BcgI	CGANNNNNNTGC	2 cut(s)	2498, 2532
BciT130I	CCWGG	5 cut(s)	368, 708, 1715, 1895, 2567
BciVI	GTATCC	1 cut(s)	780
BcnI	CCSGG	2 cut(s)	938, 2173
BcoDI	GTCTC	3 cut(s)	1410, 2036, 2273
BcuI	ACTAGT	1 cut(s)	1238
BfaI	CTAG	8 cut(s)	696, 731, 1071, 1098, 1239, 1251, 2591, 2627
BfmI	CTRYAG	5 cut(s)	384, 782, 947, 1740, 2662
BfoI	RGCGCY	2 cut(s)	1547, 2148
BfrI	CTTAAG	1 cut(s)	406
BfuAI	ACCTGC	1 cut(s)	1475
BfuI	GTATCC	1 cut(s)	780
BisI	GCNGC	6 cut(s)	201, 693, 1113, 1245, 1248, 2571
BlpI	GCTNAGC	1 cut(s)	2084
BlsI	GCNGC	6 cut(s)	202, 694, 1114, 1246, 1249, 2572
Bme1390I	CCNGG	7 cut(s)	368, 708, 938, 1715, 1895, 2173, 2567
Bme18I	GGWCC	2 cut(s)	710, 1898
BmeT110I	CYCGRG	1 cut(s)	2378
BmgT120I	GGNCC	5 cut(s)	370, 710, 1898, 2023, 2520
BmrFI	CCNGG	7 cut(s)	368, 708, 938, 1715, 1895, 2173, 2567
BmrI	ACTGGG	3 cut(s)	1615, 1673, 1952
BmsI	GCATC	6 cut(s)	1525, 1660, 1879, 1968, 2166, 2409
BmtI	GCTAGC	1 cut(s)	1074
BmuI	ACTGGG	3 cut(s)	1615, 1673, 1952
BoxI	GACNNNNGTC	1 cut(s)	2214
BpiI	GAAGAC	3 cut(s)	1041, 1763, 2210
BplI	GAGNNNNNCTC	2 cut(s)	506, 538
BpmI	CTGGAG	5 cut(s)	498, 1362, 1991, 2176, 2595
Bpu1102I	GCTNAGC	1 cut(s)	2084
Bpu14I	TTCGAA	2 cut(s)	1507, 1754
BpuEI	CTTGAG	6 cut(s)	170, 227, 659, 1895, 2095, 2226
BpuMI	CCSGG	2 cut(s)	938, 2173
BsaBI	GATNNNNATC	4 cut(s)	180, 330, 627, 1392
BsaI	GGTCTC	2 cut(s)	2036, 2273
BsaJI	CCNNGG	5 cut(s)	1714, 1894, 2026, 2426, 2595
BsaWI	WCCGGW	4 cut(s)	184, 1076, 1190, 2534
BsaXI	ACNNNNNCTCC	2 cut(s)	2547, 2577
Bsc4I	CCNNNNNNNGG	6 cut(s)	290, 999, 1000, 1969, 2079, 2413
Bse118I	RCCGGY	2 cut(s)	2079, 2534
Bse1I	ACTGG	6 cut(s)	1211, 1621, 1668, 1828, 1947, 2474
Bse3DI	GCAATG	2 cut(s)	1335, 1926
Bse8I	GATNNNNATC	4 cut(s)	180, 330, 627, 1392
BseAI	TCCGGA	1 cut(s)	184
BseBI	CCWGG	5 cut(s)	368, 708, 1715, 1895, 2567
BseDI	CCNNGG	5 cut(s)	1714, 1894, 2026, 2426, 2595
BseGI	GGATG	8 cut(s)	112, 619, 1282, 1887, 1983, 2181, 2436, 2452
BseJI	GATNNNNATC	4 cut(s)	180, 330, 627, 1392
BseLI	CCNNNNNNNGG	6 cut(s)	290, 999, 1000, 1969, 2079, 2413
BseMI	GCAATG	2 cut(s)	1335, 1926
BseMII	CTCAG	3 cut(s)	26, 1871, 2293
BseNI	ACTGG	6 cut(s)	1211, 1621, 1668, 1828, 1947, 2474
BseRI	GAGGAG	1 cut(s)	530
BseSI	GKGCMC	1 cut(s)	2256
BseXI	GCAGC	6 cut(s)	187, 704, 1099, 1234, 1256, 2557
BseYI	CCCAGC	1 cut(s)	1351
BsgI	GTGCAG	2 cut(s)	711, 1263
BshFI	GGCC	5 cut(s)	371, 521, 1022, 2024, 2521
BshNI	GGYRCC	1 cut(s)	699
BshTI	ACCGGT	1 cut(s)	2534
BsiHKAI	GWGCWC	3 cut(s)	156, 1348, 2256
BsiHKCI	CYCGRG	1 cut(s)	2378
BsiSI	CCGG	7 cut(s)	185, 937, 1077, 1191, 2080, 2172, 2535
BslFI	GGGAC	3 cut(s)	267, 1504, 2148
BslI	CCNNNNNNNGG	6 cut(s)	290, 999, 1000, 1969, 2079, 2413
BsmAI	GTCTC	3 cut(s)	1410, 2036, 2273
BsmFI	GGGAC	3 cut(s)	267, 1504, 2148
BsmI	GAATGC	1 cut(s)	1130
BsnI	GGCC	5 cut(s)	371, 521, 1022, 2024, 2521
Bso31I	GGTCTC	2 cut(s)	2036, 2273
BsoBI	CYCGRG	1 cut(s)	2378
Bsp119I	TTCGAA	2 cut(s)	1507, 1754
Bsp1286I	GDGCHC	3 cut(s)	156, 1348, 2256
Bsp13I	TCCGGA	1 cut(s)	184
Bsp1720I	GCTNAGC	1 cut(s)	2084
BspACI	CCGC	5 cut(s)	36, 1975, 2319, 2479, 2513
BspANI	GGCC	5 cut(s)	371, 521, 1022, 2024, 2521
BspCNI	CTCAG	3 cut(s)	25, 1870, 2292
BspEI	TCCGGA	1 cut(s)	184
BspHI	TCATGA	2 cut(s)	310, 454
BspMAI	CTGCAG	2 cut(s)	388, 786
BspMI	ACCTGC	1 cut(s)	1475
BspOI	GCTAGC	1 cut(s)	1074
BspQI	GCTCTTC	1 cut(s)	1217
BspT104I	TTCGAA	2 cut(s)	1507, 1754
BspT107I	GGYRCC	1 cut(s)	699
BspTI	CTTAAG	1 cut(s)	406
BspTNI	GGTCTC	2 cut(s)	2036, 2273
BsrBI	CCGCTC	1 cut(s)	1975
BsrDI	GCAATG	2 cut(s)	1335, 1926
BsrFI	RCCGGY	2 cut(s)	2079, 2534
BsrI	ACTGG	6 cut(s)	1211, 1621, 1668, 1828, 1947, 2474
BssAI	RCCGGY	2 cut(s)	2079, 2534
BssECI	CCNNGG	5 cut(s)	1714, 1894, 2026, 2426, 2595
BssNAI	GTATAC	1 cut(s)	498
BssT1I	CCWWGG	2 cut(s)	2026, 2426
Bst1107I	GTATAC	1 cut(s)	498
Bst2UI	CCWGG	5 cut(s)	368, 708, 1715, 1895, 2567
Bst4CI	ACNGT	8 cut(s)	26, 864, 1171, 1272, 2062, 2301, 2443, 2466
Bst6I	CTCTTC	4 cut(s)	117, 759, 1217, 2228
BstAFI	CTTAAG	1 cut(s)	406
BstAPI	GCANNNNNTGC	1 cut(s)	1925
BstBI	TTCGAA	2 cut(s)	1507, 1754
BstC8I	GCNNGC	8 cut(s)	1072, 1084, 1868, 2010, 2081, 2349, 2353, 2511
BstDEI	CTNAG	6 cut(s)	12, 75, 969, 1857, 2084, 2279
BstDSI	CCRYGG	1 cut(s)	2595
BstF5I	GGATG	8 cut(s)	112, 619, 1282, 1887, 1983, 2181, 2436, 2452
BstH2I	RGCGCY	2 cut(s)	1547, 2148
BstHHI	GCGC	2 cut(s)	1546, 2147
BstMAI	GTCTC	3 cut(s)	1410, 2036, 2273
BstMWI	GCNNNNNNNGC	7 cut(s)	1071, 1925, 2014, 2085, 2117, 2168, 2591
BstNI	CCWGG	5 cut(s)	368, 708, 1715, 1895, 2567
BstNSI	RCATGY	5 cut(s)	647, 675, 724, 2351, 2355
BstPAI	GACNNNNGTC	1 cut(s)	2214
BstSCI	CCNGG	7 cut(s)	366, 706, 936, 1713, 1893, 2171, 2565
BstSFI	CTRYAG	5 cut(s)	384, 782, 947, 1740, 2662
BstSLI	GKGCMC	1 cut(s)	2256
BstV1I	GCAGC	6 cut(s)	187, 704, 1099, 1234, 1256, 2557
BstV2I	GAAGAC	3 cut(s)	1041, 1763, 2210
BstX2I	RGATCY	7 cut(s)	16, 79, 175, 622, 844, 1193, 1959
BstYI	RGATCY	7 cut(s)	16, 79, 175, 622, 844, 1193, 1959
BstZ17I	GTATAC	1 cut(s)	498
BsuI	GTATCC	1 cut(s)	780
BsuRI	GGCC	5 cut(s)	371, 521, 1022, 2024, 2521
BtgI	CCRYGG	1 cut(s)	2595
BtgZI	GCGATG	1 cut(s)	2432
BtsCI	GGATG	8 cut(s)	112, 619, 1282, 1887, 1983, 2181, 2436, 2452
BtsI	GCAGTG	1 cut(s)	1346
BtsIMutI	CAGTG	7 cut(s)	31, 1346, 1865, 2448, 2462, 2475, 2481
BveI	ACCTGC	1 cut(s)	1475
Cac8I	GCNNGC	8 cut(s)	1072, 1084, 1868, 2010, 2081, 2349, 2353, 2511
CciI	TCATGA	2 cut(s)	310, 454
CfoI	GCGC	2 cut(s)	1546, 2147
Cfr10I	RCCGGY	2 cut(s)	2079, 2534
Cfr13I	GGNCC	5 cut(s)	370, 710, 1898, 2023, 2520
CseI	GACGC	2 cut(s)	269, 494
Csp6I	GTAC	7 cut(s)	343, 700, 840, 1172, 1841, 2386, 2401
CspAI	ACCGGT	1 cut(s)	2534
CviQI	GTAC	7 cut(s)	343, 700, 840, 1172, 1841, 2386, 2401
DdeI	CTNAG	6 cut(s)	12, 75, 969, 1857, 2084, 2279
Eam1104I	CTCTTC	4 cut(s)	117, 759, 1217, 2228
EarI	CTCTTC	4 cut(s)	117, 759, 1217, 2228
Ecl136II	GAGCTC	1 cut(s)	154
Eco130I	CCWWGG	2 cut(s)	2026, 2426
Eco147I	AGGCCT	1 cut(s)	521
Eco24I	GRGCYC	1 cut(s)	156
Eco31I	GGTCTC	2 cut(s)	2036, 2273
Eco32I	GATATC	2 cut(s)	355, 986
Eco47I	GGWCC	2 cut(s)	710, 1898
Eco53kI	GAGCTC	1 cut(s)	154
Eco57I	CTGAAG	8 cut(s)	141, 237, 872, 1053, 1161, 1767, 1956, 2477
Eco88I	CYCGRG	1 cut(s)	2378
EcoICRI	GAGCTC	1 cut(s)	154
EcoRII	CCWGG	5 cut(s)	366, 706, 1713, 1893, 2565
EcoRV	GATATC	2 cut(s)	355, 986
EcoT14I	CCWWGG	2 cut(s)	2026, 2426
EcoT22I	ATGCAT	2 cut(s)	1132, 2353
EcoT38I	GRGCYC	1 cut(s)	156
ErhI	CCWWGG	2 cut(s)	2026, 2426
FalI	AAGNNNNNCTT	8 cut(s)	1037, 1069, 1533, 1565, 1836, 1868, 2255, 2287
FaqI	GGGAC	3 cut(s)	267, 1504, 2148
FauI	CCCGC	2 cut(s)	2472, 2506
FblI	GTMKAC	2 cut(s)	497, 648
Fnu4HI	GCNGC	6 cut(s)	201, 693, 1113, 1245, 1248, 2571
FokI	GGATG	8 cut(s)	119, 626, 1289, 1874, 1990, 2188, 2443, 2459
FriOI	GRGCYC	1 cut(s)	156
Fsp4HI	GCNGC	6 cut(s)	201, 693, 1113, 1245, 1248, 2571
FspBI	CTAG	8 cut(s)	696, 731, 1071, 1098, 1239, 1251, 2591, 2627
GlaI	GCGC	2 cut(s)	1545, 2146
GluI	GCNGC	6 cut(s)	201, 693, 1113, 1245, 1248, 2571
GsaI	CCCAGC	1 cut(s)	1355
GsuI	CTGGAG	5 cut(s)	498, 1362, 1991, 2176, 2595
HaeII	RGCGCY	2 cut(s)	1547, 2148
HaeIII	GGCC	5 cut(s)	371, 521, 1022, 2024, 2521
HapII	CCGG	7 cut(s)	185, 937, 1077, 1191, 2080, 2172, 2535
HgaI	GACGC	2 cut(s)	269, 494
HhaI	GCGC	2 cut(s)	1546, 2147
Hin6I	GCGC	2 cut(s)	1544, 2145
HinP1I	GCGC	2 cut(s)	1544, 2145
HincII	GTYRAC	2 cut(s)	961, 2439
HindII	GTYRAC	2 cut(s)	961, 2439
HindIII	AAGCTT	1 cut(s)	403
HpaI	GTTAAC	1 cut(s)	961
HpaII	CCGG	7 cut(s)	185, 937, 1077, 1191, 2080, 2172, 2535
HphI	GGTGA	5 cut(s)	404, 446, 1679, 1895, 2068
Hpy166II	GTNNAC	6 cut(s)	498, 649, 961, 1282, 2254, 2439
Hpy8I	GTNNAC	6 cut(s)	498, 649, 961, 1282, 2254, 2439
Hpy99I	CGWCG	1 cut(s)	1064
HpyCH4III	ACNGT	8 cut(s)	26, 864, 1171, 1272, 2062, 2301, 2443, 2466
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1071, 1925, 2014, 2085, 2117, 2168, 2591
HpyF3I	CTNAG	6 cut(s)	12, 75, 969, 1857, 2084, 2279
HspAI	GCGC	2 cut(s)	1544, 2145
Kpn2I	TCCGGA	1 cut(s)	184
KpnI	GGTACC	1 cut(s)	703
KroI	GCCGGC	1 cut(s)	2079
KroNI	GCCGGC	1 cut(s)	2081
KspAI	GTTAAC	1 cut(s)	961
LguI	GCTCTTC	1 cut(s)	1217
LmnI	GCTCC	5 cut(s)	10, 541, 1079, 1343, 1972
Lsp1109I	GCAGC	6 cut(s)	187, 704, 1099, 1234, 1256, 2557
LweI	GCATC	6 cut(s)	1525, 1660, 1879, 1968, 2166, 2409
MaeI	CTAG	8 cut(s)	696, 731, 1071, 1098, 1239, 1251, 2591, 2627
MaeIII	GTNAC	5 cut(s)	434, 1604, 1883, 2056, 2335
MbiI	CCGCTC	1 cut(s)	1975
MflI	RGATCY	7 cut(s)	16, 79, 175, 622, 844, 1193, 1959
MhlI	GDGCHC	3 cut(s)	156, 1348, 2256
MluCI	AATT	9 cut(s)	188, 469, 555, 658, 1105, 1369, 1473, 1514, 2121
MlyI	GAGTC	3 cut(s)	1175, 1277, 2224
MmeI	TCCRAC	2 cut(s)	10, 32
Mph1103I	ATGCAT	2 cut(s)	1132, 2353
MroI	TCCGGA	1 cut(s)	184
MroNI	GCCGGC	1 cut(s)	2079
MroXI	GAANNNNTTC	2 cut(s)	863, 1760
MseI	TTAA	9 cut(s)	300, 407, 536, 608, 819, 960, 974, 1380, 2645
MslI	CAYNNNNRTG	2 cut(s)	459, 1320
MspA1I	CMGCKG	3 cut(s)	1247, 1745, 1904
MspCI	CTTAAG	1 cut(s)	406
MspI	CCGG	7 cut(s)	185, 937, 1077, 1191, 2080, 2172, 2535
MspR9I	CCNGG	7 cut(s)	368, 708, 938, 1715, 1895, 2173, 2567
Mva1269I	GAATGC	1 cut(s)	1130
MvaI	CCWGG	5 cut(s)	368, 708, 1715, 1895, 2567
MwoI	GCNNNNNNNGC	7 cut(s)	1071, 1925, 2014, 2085, 2117, 2168, 2591
NaeI	GCCGGC	1 cut(s)	2081
NciI	CCSGG	2 cut(s)	938, 2173
NgoMIV	GCCGGC	1 cut(s)	2079
NheI	GCTAGC	1 cut(s)	1070
NmuCI	GTSAC	4 cut(s)	434, 1883, 2056, 2335
NsiI	ATGCAT	2 cut(s)	1132, 2353
NspI	RCATGY	5 cut(s)	647, 675, 724, 2351, 2355
NspV	TTCGAA	2 cut(s)	1507, 1754
PaeI	GCATGC	2 cut(s)	2351, 2355
PagI	TCATGA	2 cut(s)	310, 454
PceI	AGGCCT	1 cut(s)	521
PciI	ACATGT	1 cut(s)	720
PciSI	GCTCTTC	1 cut(s)	1217
PctI	GAATGC	1 cut(s)	1130
PdiI	GCCGGC	1 cut(s)	2081
PdmI	GAANNNNTTC	2 cut(s)	863, 1760
PfeI	GAWTC	8 cut(s)	326, 1091, 1264, 1436, 1449, 1504, 2099, 2634
PfoI	TCCNGGA	1 cut(s)	706
PinAI	ACCGGT	1 cut(s)	2534
PkrI	GCNGC	6 cut(s)	202, 694, 1114, 1246, 1249, 2572
PleI	GAGTC	3 cut(s)	1175, 1277, 2223
PpsI	GAGTC	3 cut(s)	1175, 1277, 2223
PscI	ACATGT	1 cut(s)	720
PshAI	GACNNNNGTC	1 cut(s)	2214
Psp124BI	GAGCTC	1 cut(s)	156
Psp6I	CCWGG	5 cut(s)	366, 706, 1713, 1893, 2565
PspFI	CCCAGC	1 cut(s)	1351
PspGI	CCWGG	5 cut(s)	366, 706, 1713, 1893, 2565
PspPI	GGNCC	5 cut(s)	370, 710, 1898, 2023, 2520
PsrI	GAACNNNNNNTAC	1 cut(s)	2645
PstI	CTGCAG	2 cut(s)	388, 786
PsuI	RGATCY	7 cut(s)	16, 79, 175, 622, 844, 1193, 1959
PvuII	CAGCTG	3 cut(s)	1247, 1745, 1904
RsaI	GTAC	7 cut(s)	344, 701, 841, 1173, 1842, 2387, 2402
RsaNI	GTAC	7 cut(s)	343, 700, 840, 1172, 1841, 2386, 2401
RseI	CAYNNNNRTG	2 cut(s)	459, 1320
SacI	GAGCTC	1 cut(s)	156
SapI	GCTCTTC	1 cut(s)	1217
SaqAI	TTAA	9 cut(s)	300, 407, 536, 608, 819, 960, 974, 1380, 2645
SatI	GCNGC	6 cut(s)	201, 693, 1113, 1245, 1248, 2571
Sau96I	GGNCC	5 cut(s)	370, 710, 1898, 2023, 2520
SchI	GAGTC	3 cut(s)	1175, 1277, 2224
ScrFI	CCNGG	7 cut(s)	368, 708, 938, 1715, 1895, 2173, 2567
SduI	GDGCHC	3 cut(s)	156, 1348, 2256
SfaNI	GCATC	6 cut(s)	1525, 1660, 1879, 1968, 2166, 2409
SfcI	CTRYAG	5 cut(s)	384, 782, 947, 1740, 2662
SfuI	TTCGAA	2 cut(s)	1507, 1754
SinI	GGWCC	2 cut(s)	710, 1898
SmiMI	CAYNNNNRTG	2 cut(s)	459, 1320
SmlI	CTYRAG	7 cut(s)	149, 206, 406, 638, 1874, 2074, 2241
SmoI	CTYRAG	7 cut(s)	149, 206, 406, 638, 1874, 2074, 2241
SpeI	ACTAGT	1 cut(s)	1238
SphI	GCATGC	2 cut(s)	2351, 2355
Sse9I	AATT	9 cut(s)	188, 469, 555, 658, 1105, 1369, 1473, 1514, 2121
SseBI	AGGCCT	1 cut(s)	521
SsiI	CCGC	5 cut(s)	36, 1975, 2319, 2479, 2513
SspI	AATATT	1 cut(s)	1310
SspMI	CTAG	8 cut(s)	696, 731, 1071, 1098, 1239, 1251, 2591, 2627
SstI	GAGCTC	1 cut(s)	156
StuI	AGGCCT	1 cut(s)	521
StyD4I	CCNGG	7 cut(s)	366, 706, 936, 1713, 1893, 2171, 2565
StyI	CCWWGG	2 cut(s)	2026, 2426
TaaI	ACNGT	8 cut(s)	26, 864, 1171, 1272, 2062, 2301, 2443, 2466
TaqI	TCGA	5 cut(s)	1059, 1302, 1507, 1648, 1754
TasI	AATT	9 cut(s)	188, 469, 555, 658, 1105, 1369, 1473, 1514, 2121
TatI	WGTACW	1 cut(s)	2400
TfiI	GAWTC	8 cut(s)	326, 1091, 1264, 1436, 1449, 1504, 2099, 2634
Tru1I	TTAA	9 cut(s)	300, 407, 536, 608, 819, 960, 974, 1380, 2645
Tru9I	TTAA	9 cut(s)	300, 407, 536, 608, 819, 960, 974, 1380, 2645
TscAI	CASTG	7 cut(s)	31, 1353, 1865, 2448, 2469, 2475, 2481
TseFI	GTSAC	4 cut(s)	434, 1883, 2056, 2335
TseI	GCWGC	6 cut(s)	200, 692, 1112, 1244, 1247, 2570
Tsp45I	GTSAC	4 cut(s)	434, 1883, 2056, 2335
TspGWI	ACGGA	5 cut(s)	60, 857, 1952, 2004, 2612
TspRI	CASTG	7 cut(s)	31, 1353, 1865, 2448, 2469, 2475, 2481
Vha464I	CTTAAG	1 cut(s)	406
VneI	GTGCAC	1 cut(s)	2252
VpaK11BI	GGWCC	2 cut(s)	710, 1898
XapI	RAATTY	4 cut(s)	469, 658, 1105, 1473
XbaI	TCTAGA	1 cut(s)	730
XceI	RCATGY	5 cut(s)	647, 675, 724, 2351, 2355
XmiI	GTMKAC	2 cut(s)	497, 648
XmnI	GAANNNNTTC	2 cut(s)	863, 1760
XspI	CTAG	8 cut(s)	696, 731, 1071, 1098, 1239, 1251, 2591, 2627
Zsp2I	ATGCAT	2 cut(s)	1132, 2353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.