Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010697

Physical Location & Seq

Genomic Coordinates Forward (+)

28351218 .. 28360470

Gene Structure 9253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1234500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGGCACCTCAAGATCCAAACGCCCCAAGCGGCAGCGGCTCCGGCAAGCGCAGTGAGGATTCCGTTAAAGTCCCCGCAACAGATCCCAAGAAGAAAGATGATAAGAAGGATGAGGATTTGTCTGAGGAGGATTTGGAGTTGAAGAACCAATTAGAGTTGTACGTCGAGAGGGTTCAGGATACTGATCCTGGTGTGCAGAAAAATGCTCTGGAGAGTATGAGGCAGGAAATCCGTACCTCAACTAGCTCCATGACTTCAGTTCCAAAGCCATTAAAGTATCTTCGCCCGCATTACGGAACTCTAAAAACTTACTATGAAACAATGGCAGAATCAGAGTTGAAGAAATACCTAGCAGATATATTGTCCGTTTTGGCACTCACCATGGCAGCTGAGGGAGAACGGGAAAGCTTGAAGTACAGATTGTTGGGTTCAGAGGGTGATATTGGTTCATGGGGACACGAATATGTCAGGAACTTAGCTGGAGAAATTGCTCAGGAGTTTGCTAAGCGACAGGGTGAAGAGGCCCCAATTGAAGAACTAATGGAACTAGTGCAGCAAATTGTTGCTTTTCACATGAAGCACAATGCTGAACCTGAAGCTGTCGACCTTTTAATGGAGGTTGAAGACCTTGATCTGTTAATTGAGCATGTTGATAAAACAAATTTTAAGAGGACCTGTCTCTACCTCACCAGTTCAGCAAGATACCTTCCTGGACCAGATGATGTTGTAGTTCTGGATATTGCATACACAATATATATGAAATTTGAAGAATATCCAAATGCACTTCAGATTGCACTGTTCCTTGATAACATGCAGAATATAAAGAATGTCTTTACATCATGTGATGATCTGCTTCGTAAAAAGCAATTTTGCTACATCCTTGCTCGACATGGTATTAGTTTTGAGCTTGATGATGAGATGGTTGCGGAGGATGATGACAGGGAAGTATTGCAGGACATTATCAACAATACTAAGCTAAGTGAAGGTTATCTTACTCTTGCTCGTGATATTGAGGTCATGGAGGCCAAAACTCCAGAGGATATCTACAAGGCACACTTACTTGATGGCCGTGCAAGTGCTGGTGCTAGTGTTGATTCAGCAAGACAGAACTTGGCTGCAACATTTGTTAACGCTTTTGTAAATGCTGGATTTGGTCAGGATAAGTTGATGACAGTCCCATCAGATGCCTCAAGTGGAGGCTCTTCAGGAAACTGGCTTTTCAAGAATAAAGAGCATGGGAAGACAAGTGCTGCAGCAAGTCTGGGTATGATTTTATTGTGGGATGTTGACTCTGGACTTGCCCAAATTGACAAGTACTTCCACAGCAACGATACCCATGTTATCGCTGGTGCATTATTAGGGGTTGGGATTGTTAATTGTGGTATCAAGAATGATTGTGATCCTGCGCTGGCACTTCTCGGCGAGTACATTGATAGAGAGGATCCATCTATCCGGATTGGTGCTATAATGGGCCTTGGGATTGCATATGCTGGTTCTCAGAACGAGCAGATACGTAGTAAGCTGACTCCTATACTTAATGATGCAAAAGCCCCCCTTGACGTGGTCGCATTTACTGCAATCTCATTGGGGTTGGTATACGTTGGTTCCTGCAATGAAGAGGTTGCTCAGGCAATCATATTTGCACTAATGGACCGAAGTGACTCCGAGTTGGGGGAGCCCCTTGCTCGGTTGATTCCCCTCAGCCTTGGACTTCTGTACCTTGGGAAGCAGGAAAGTGTGGAGGCCACTGCCGAAGTTTCAAAGACATTTAATGAAAAAATAAGAAAGCATTGTGATATGACTTTGCTTTCTTGTGCTTATGCAGGAACAGGCAATGTTCTTAAGGTCCAAAACCTTCTCGGTCATTGTTCACAACATCTTGATAAGAATGAAACTCACCAGGGACCCGCAGTTCTTGGAATTGCTATGATATCAATGGCTGAAGAATTGGGAGTTGAAATGGCGATTCGTTCATTAGAGCATCTTTTACAGTACGGAGAACAAAATATACGTCGGGCAGTTCCATTGGCACTTGGTCTCCTTTGCATATCAAATCCAAAGGTCAACGTTATGGACACATTAAGCAGGCTTAGCCACGATACGGATTCAGAAGTAGCAATGGCTGCAGTGATTTCCTTAGGTTTGCTAGGTGCGGGAACCAACAACGCTAGAATAGCTGGCATGCTTCGCAATCTTTCAAGTTATTACTACAAAGATGCCAGCCTTCTTTTCTGTGTGCGAATTGCTCAAGGTCTTGTTCATTTAGGAAAGGGTTTGTTAACTCTGAATCCATACCATTCTGATCGCTTCTTGCTATCACCAACGGCACTTGCTGGAATCGTTACCGTGCTGCATGCATGTCTTGATATGAAAGCCATCATTCTGGGGAAATATCATTACATTCTCTACTTTATTACTTTGGCAATGCAGCCGAGAATGTTGTTGACTGTGGACGAGAATCTCAAACCGCTGTCAGTCCCTGTTCGAGTGGGACAAGCTGTTGATGTGGTTGGCCAGGCTGGTCGTCCCAAAACGATCACGGGTTTCCAGACCCACTCAACACCGGTTCTTCTTGCTGCGGGTGATAGAGCGGAGCTGGCTACTGAAAAGTATATCCCCCTGTCTCCCATCTTGGAAGGGTTCGTTATCCTGAAAGAGAATCCAGACTACAGAGAAGATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0001101 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010498 GO:0014070 GO:0016020 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030163 GO:0031597 GO:0032182 GO:0032991 GO:0034515 GO:0042221 GO:0042493 GO:0043130 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051726 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PC_rep

Protein Analysis

895

Amino Acids

98.12

Weight (kDa)

5.03

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN1_RPN2_N	PF17781	51 - 353	2.1e-127	RPN1 N-terminal domain
PC_rep	PF01851	487 - 515	6.8e-06	Proteasome/cyclosome repeat
RPN1_C	PF18051	837 - 890	2.3e-31	26S proteasome non-ATPase regulatory subunit RPN1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20580 AT4G28470
fragaria_vesca	FvH4_4g14560
malus_domestica	MD09G1242100.v1.1 MD17G1234500.v1.1
prunus_persica	Prupe.3G134800_v2.0.a1
pyrus_communis	pycom09g16070
rosa_chinensis	RchiOBHm_Chr4g0418261
rosa_laevigata	RLG00000007882 RLG00000014797
rosa_multiflora	Rmu_co8313613.1_g000001 Rmu_sc0000032.1_g000014
rosa_roxburghii	Rroxscaffold_5G00361020 Rroxscaffold_5G00361440
rosa_rugosa	Rorug04G0151500
rosa_samantha	Rh4BG217500 Rh4CG224800 Rh4DG211700
rosa_wichuraiana	Rw4G018100 Rw6G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	4
AccBSI	CCGCTC	1 cut(s)	2591
AccI	GTMKAC	2 cut(s)	603, 1596
AccIII	TCCGGA	1 cut(s)	1452
AclI	AACGTT	1 cut(s)	2067
AclWI	GGATC	6 cut(s)	8, 77, 179, 1394, 1436, 1449
AcoI	YGGCCR	2 cut(s)	1066, 2512
AcsI	RAATTY	2 cut(s)	661, 761
AcuI	CTGAAG	5 cut(s)	240, 615, 770, 1188, 1962
AfaI	GTAC	7 cut(s)	161, 235, 416, 1316, 1427, 1718, 1994
AfiI	CCNNNNNNNGG	6 cut(s)	293, 613, 1561, 1671, 1686, 2101
AflII	CTTAAG	1 cut(s)	1841
AgeI	ACCGGT	1 cut(s)	2563
AhlI	ACTAGT	1 cut(s)	547
AjiI	CACGTC	1 cut(s)	1561
AjnI	CCWGG	4 cut(s)	187, 709, 1899, 2514
AloI	GAACNNNNNNTCC	2 cut(s)	1896, 1928
Alw26I	GTCTC	3 cut(s)	683, 2042, 2628
AlwI	GGATC	6 cut(s)	8, 77, 179, 1394, 1436, 1449
AlwNI	CAGNNNCTG	1 cut(s)	2480
Aor13HI	TCCGGA	1 cut(s)	1452
AoxI	GGCC	6 cut(s)	522, 1023, 1066, 1471, 1743, 2512
ApoI	RAATTY	2 cut(s)	661, 761
ArsI	GACNNNNNNTTYG	2 cut(s)	1996, 2028
AsiGI	ACCGGT	1 cut(s)	2563
AspLEI	GCGC	2 cut(s)	51, 1408
AspS9I	GGNCC	7 cut(s)	523, 672, 713, 1471, 1651, 1846, 1904
AsuHPI	GGTGA	7 cut(s)	370, 449, 527, 679, 1889, 2310, 2594
AvaII	GGWCC	5 cut(s)	672, 713, 1651, 1846, 1904
AxyI	CCTNAGG	1 cut(s)	2137
BaeI	ACNNNNGTAYC	4 cut(s)	1323, 1356, 1700, 1733
BalI	TGGCCA	1 cut(s)	2514
BamHI	GGATCC	1 cut(s)	1441
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	1 cut(s)	1680
BauI	CACGAG	1 cut(s)	1002
BbsI	GAAGAC	2 cut(s)	630, 1247
BbvCI	CCTCAGC	2 cut(s)	390, 1700
BccI	CCATC	6 cut(s)	913, 1058, 1186, 1453, 2384, 2636
BceAI	ACGGC	2 cut(s)	1053, 2340
BciT130I	CCWGG	4 cut(s)	189, 711, 1901, 2516
BciVI	GTATCC	1 cut(s)	172
BcoDI	GTCTC	3 cut(s)	683, 2042, 2628
BcuI	ACTAGT	1 cut(s)	547
BfaI	CTAG	7 cut(s)	243, 350, 548, 1086, 2147, 2169, 2683
BfmI	CTRYAG	3 cut(s)	1251, 2124, 2668
BfrI	CTTAAG	1 cut(s)	1841
BfuI	GTATCC	1 cut(s)	172
BlpI	GCTNAGC	2 cut(s)	504, 2090
BmcAI	AGTACT	1 cut(s)	1316
Bme1390I	CCNGG	4 cut(s)	189, 711, 1901, 2516
Bme18I	GGWCC	5 cut(s)	672, 713, 1651, 1846, 1904
BmgBI	CACGTC	1 cut(s)	1561
BmgT120I	GGNCC	7 cut(s)	523, 672, 713, 1471, 1651, 1846, 1904
BmiI	GGNNCC	9 cut(s)	6, 40, 525, 1443, 1606, 1677, 1905, 1906, 2158
BmrFI	CCNGG	4 cut(s)	189, 711, 1901, 2516
BmsI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
BpiI	GAAGAC	2 cut(s)	630, 1247
BpmI	CTGGAG	3 cut(s)	230, 501, 1017
Bpu10I	CCTNAGC	4 cut(s)	390, 492, 1626, 1700
Bpu1102I	GCTNAGC	2 cut(s)	504, 2090
BpuEI	CTTGAG	2 cut(s)	1174, 2232
BsaAI	YACGTR	1 cut(s)	1514
BsaBI	GATNNNNATC	2 cut(s)	183, 1398
BsaI	GGTCTC	1 cut(s)	2042
BsaJI	CCNNGG	5 cut(s)	381, 1474, 1705, 1720, 1900
BsaWI	WCCGGW	2 cut(s)	1452, 2563
BsaXI	ACNNNNNCTCC	2 cut(s)	2022, 2052
Bsc4I	CCNNNNNNNGG	6 cut(s)	293, 613, 1561, 1671, 1686, 2101
Bse118I	RCCGGY	1 cut(s)	2563
Bse1I	ACTGG	2 cut(s)	690, 1217
Bse21I	CCTNAGG	1 cut(s)	2137
Bse3DI	GCAATG	4 cut(s)	1618, 1840, 2124, 2430
Bse8I	GATNNNNATC	2 cut(s)	183, 1398
BseAI	TCCGGA	1 cut(s)	1452
BseBI	CCWGG	4 cut(s)	189, 711, 1901, 2516
BseDI	CCNNGG	5 cut(s)	381, 1474, 1705, 1720, 1900
BseGI	GGATG	4 cut(s)	115, 876, 937, 1288
BseJI	GATNNNNATC	2 cut(s)	183, 1398
BseLI	CCNNNNNNNGG	6 cut(s)	293, 613, 1561, 1671, 1686, 2101
BseMI	GCAATG	4 cut(s)	1618, 1840, 2124, 2430
BseMII	CTCAG	6 cut(s)	114, 381, 506, 1511, 1640, 1714
BseNI	ACTGG	2 cut(s)	690, 1217
BseRI	GAGGAG	1 cut(s)	140
BsgI	GTGCAG	2 cut(s)	215, 572
BshFI	GGCC	6 cut(s)	524, 1025, 1068, 1473, 1745, 2514
BshNI	GGYRCC	1 cut(s)	4
BshTI	ACCGGT	1 cut(s)	2563
BsiSI	CCGG	3 cut(s)	42, 1453, 2564
BslFI	GGGAC	7 cut(s)	56, 468, 1160, 1917, 2462, 2505, 2511
BslI	CCNNNNNNNGG	6 cut(s)	293, 613, 1561, 1671, 1686, 2101
BsmAI	GTCTC	3 cut(s)	683, 2042, 2628
BsmFI	GGGAC	7 cut(s)	56, 468, 1160, 1917, 2462, 2505, 2511
BsnI	GGCC	6 cut(s)	524, 1025, 1068, 1473, 1745, 2514
Bso31I	GGTCTC	1 cut(s)	2042
Bsp1286I	GDGCHC	1 cut(s)	1680
Bsp13I	TCCGGA	1 cut(s)	1452
Bsp143I	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
Bsp1720I	GCTNAGC	2 cut(s)	504, 2090
Bsp19I	CCATGG	1 cut(s)	381
BspANI	GGCC	6 cut(s)	524, 1025, 1068, 1473, 1745, 2514
BspCNI	CTCAG	6 cut(s)	115, 382, 505, 1510, 1639, 1713
BspEI	TCCGGA	1 cut(s)	1452
BspLI	GGNNCC	9 cut(s)	6, 40, 525, 1443, 1606, 1677, 1905, 1906, 2158
BspMAI	CTGCAG	2 cut(s)	1255, 2128
BspPI	GGATC	6 cut(s)	8, 77, 179, 1394, 1436, 1449
BspQI	GCTCTTC	1 cut(s)	1207
BspT107I	GGYRCC	1 cut(s)	4
BspTI	CTTAAG	1 cut(s)	1841
BspTNI	GGTCTC	1 cut(s)	2042
BsrBI	CCGCTC	1 cut(s)	2591
BsrDI	GCAATG	4 cut(s)	1618, 1840, 2124, 2430
BsrFI	RCCGGY	1 cut(s)	2563
BsrI	ACTGG	2 cut(s)	690, 1217
BssAI	RCCGGY	1 cut(s)	2563
BssECI	CCNNGG	5 cut(s)	381, 1474, 1705, 1720, 1900
BssMI	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
BssNAI	GTATAC	1 cut(s)	1597
BssSI	CACGAG	1 cut(s)	1002
BssT1I	CCWWGG	4 cut(s)	381, 1474, 1705, 1720
Bst1107I	GTATAC	1 cut(s)	1597
Bst2BI	CACGAG	1 cut(s)	1002
Bst2UI	CCWGG	4 cut(s)	189, 711, 1901, 2516
Bst4CI	ACNGT	5 cut(s)	798, 1174, 1992, 2347, 2449
Bst6I	CTCTTC	3 cut(s)	513, 1207, 1611
BstAFI	CTTAAG	1 cut(s)	1841
BstAPI	GCANNNNNTGC	2 cut(s)	1574, 2123
BstBAI	YACGTR	1 cut(s)	1514
BstC8I	GCNNGC	9 cut(s)	47, 287, 1410, 2087, 2179, 2183, 2221, 2355, 2598
BstDSI	CCRYGG	1 cut(s)	381
BstF5I	GGATG	4 cut(s)	115, 876, 937, 1288
BstHHI	GCGC	2 cut(s)	51, 1408
BstKTI	GATC	9 cut(s)	16, 85, 187, 634, 850, 1402, 1444, 2305, 2538
BstMAI	GTCTC	3 cut(s)	683, 2042, 2628
BstMBI	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
BstMWI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
BstNI	CCWGG	4 cut(s)	189, 711, 1901, 2516
BstNSI	RCATGY	5 cut(s)	650, 814, 2185, 2357, 2361
BstSCI	CCNGG	4 cut(s)	187, 709, 1899, 2514
BstSFI	CTRYAG	3 cut(s)	1251, 2124, 2668
BstSNI	TACGTA	1 cut(s)	1514
BstV2I	GAAGAC	2 cut(s)	630, 1247
BstX2I	RGATCY	3 cut(s)	13, 82, 1441
BstXI	CCANNNNNNTGG	1 cut(s)	2383
BstYI	RGATCY	3 cut(s)	13, 82, 1441
BstZ17I	GTATAC	1 cut(s)	1597
Bsu36I	CCTNAGG	1 cut(s)	2137
BsuI	GTATCC	1 cut(s)	172
BsuRI	GGCC	6 cut(s)	524, 1025, 1068, 1473, 1745, 2514
BtgI	CCRYGG	1 cut(s)	381
BtrI	CACGTC	1 cut(s)	1561
BtsCI	GGATG	4 cut(s)	115, 876, 937, 1288
BtsI	GCAGTG	3 cut(s)	58, 1746, 2133
BtsIMutI	CAGTG	4 cut(s)	58, 794, 1746, 2133
Cac8I	GCNNGC	9 cut(s)	47, 287, 1410, 2087, 2179, 2183, 2221, 2355, 2598
CaiI	CAGNNNCTG	1 cut(s)	2480
CfoI	GCGC	2 cut(s)	51, 1408
Cfr10I	RCCGGY	1 cut(s)	2563
Cfr13I	GGNCC	7 cut(s)	523, 672, 713, 1471, 1651, 1846, 1904
Csp6I	GTAC	7 cut(s)	160, 234, 415, 1315, 1426, 1717, 1993
CspAI	ACCGGT	1 cut(s)	2563
CviQI	GTAC	7 cut(s)	160, 234, 415, 1315, 1426, 1717, 1993
DpnI	GATC	9 cut(s)	15, 84, 186, 633, 849, 1401, 1443, 2304, 2537
DpnII	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
EaeI	YGGCCR	2 cut(s)	1066, 2512
Eam1104I	CTCTTC	3 cut(s)	513, 1207, 1611
EarI	CTCTTC	3 cut(s)	513, 1207, 1611
Eco105I	TACGTA	1 cut(s)	1514
Eco130I	CCWWGG	4 cut(s)	381, 1474, 1705, 1720
Eco24I	GRGCYC	1 cut(s)	1680
Eco31I	GGTCTC	1 cut(s)	2042
Eco32I	GATATC	2 cut(s)	1042, 1932
Eco47I	GGWCC	5 cut(s)	672, 713, 1651, 1846, 1904
Eco57I	CTGAAG	5 cut(s)	240, 615, 770, 1188, 1962
Eco81I	CCTNAGG	1 cut(s)	2137
EcoO109I	RGGNCCY	3 cut(s)	523, 672, 1904
EcoRII	CCWGG	4 cut(s)	187, 709, 1899, 2514
EcoRV	GATATC	2 cut(s)	1042, 1932
EcoT14I	CCWWGG	4 cut(s)	381, 1474, 1705, 1720
EcoT22I	ATGCAT	1 cut(s)	2359
EcoT38I	GRGCYC	1 cut(s)	1680
ErhI	CCWWGG	4 cut(s)	381, 1474, 1705, 1720
FalI	AAGNNNNNCTT	8 cut(s)	815, 847, 975, 1007, 1040, 1072, 1539, 1571
FaqI	GGGAC	7 cut(s)	56, 468, 1160, 1917, 2462, 2505, 2511
FauI	CCCGC	5 cut(s)	82, 294, 1915, 2146, 2572
FauNDI	CATATG	1 cut(s)	1486
FblI	GTMKAC	2 cut(s)	603, 1596
FokI	GGATG	4 cut(s)	122, 863, 944, 1295
FriOI	GRGCYC	1 cut(s)	1680
FspBI	CTAG	7 cut(s)	243, 350, 548, 1086, 2147, 2169, 2683
GlaI	GCGC	2 cut(s)	50, 1407
GsuI	CTGGAG	3 cut(s)	230, 501, 1017
HaeIII	GGCC	6 cut(s)	524, 1025, 1068, 1473, 1745, 2514
HapII	CCGG	3 cut(s)	42, 1453, 2564
HhaI	GCGC	2 cut(s)	51, 1408
Hin6I	GCGC	2 cut(s)	49, 1406
HinP1I	GCGC	2 cut(s)	49, 1406
HincII	GTYRAC	6 cut(s)	604, 1129, 1288, 2065, 2280, 2445
HindII	GTYRAC	6 cut(s)	604, 1129, 1288, 2065, 2280, 2445
HindIII	AAGCTT	1 cut(s)	406
HpaI	GTTAAC	2 cut(s)	1129, 2280
HpaII	CCGG	3 cut(s)	42, 1453, 2564
HphI	GGTGA	7 cut(s)	370, 449, 527, 679, 1889, 2310, 2594
Hpy166II	GTNNAC	9 cut(s)	604, 1129, 1288, 1597, 1871, 2065, 2280, 2445, 2452
Hpy8I	GTNNAC	9 cut(s)	604, 1129, 1288, 1597, 1871, 2065, 2280, 2445, 2452
Hpy99I	CGWCG	2 cut(s)	167, 2016
HpyAV	CCTTC	6 cut(s)	100, 716, 977, 1865, 2234, 2630
HpyCH4III	ACNGT	5 cut(s)	798, 1174, 1992, 2347, 2449
HpyCH4IV	ACGT	6 cut(s)	162, 1513, 1560, 1599, 2011, 2067
HpyF10VI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
HpySE526I	ACGT	6 cut(s)	162, 1513, 1560, 1599, 2011, 2067
HspAI	GCGC	2 cut(s)	49, 1406
KflI	GGGWCCC	1 cut(s)	1904
Kpn2I	TCCGGA	1 cut(s)	1452
KspAI	GTTAAC	2 cut(s)	1129, 2280
Kzo9I	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
LguI	GCTCTTC	1 cut(s)	1207
LmnI	GCTCC	4 cut(s)	44, 251, 1675, 2593
LweI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
MaeI	CTAG	7 cut(s)	243, 350, 548, 1086, 2147, 2169, 2683
MaeII	ACGT	6 cut(s)	162, 1513, 1560, 1599, 2011, 2067
MaeIII	GTNAC	2 cut(s)	1658, 2341
MalI	GATC	9 cut(s)	15, 84, 186, 633, 849, 1401, 1443, 2304, 2537
MbiI	CCGCTC	1 cut(s)	2591
MboI	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
MfeI	CAATTG	1 cut(s)	528
MflI	RGATCY	3 cut(s)	13, 82, 1441
MhlI	GDGCHC	1 cut(s)	1680
MlsI	TGGCCA	1 cut(s)	2514
MluNI	TGGCCA	1 cut(s)	2514
MlyI	GAGTC	3 cut(s)	1283, 1519, 1655
Mox20I	TGGCCA	1 cut(s)	2514
Mph1103I	ATGCAT	1 cut(s)	2359
MroI	TCCGGA	1 cut(s)	1452
MscI	TGGCCA	1 cut(s)	2514
MslI	CAYNNNNRTG	1 cut(s)	462
Msp20I	TGGCCA	1 cut(s)	2514
MspA1I	CMGCKG	3 cut(s)	36, 389, 2470
MspCI	CTTAAG	1 cut(s)	1841
MspI	CCGG	3 cut(s)	42, 1453, 2564
MspR9I	CCNGG	4 cut(s)	189, 711, 1901, 2516
MunI	CAATTG	1 cut(s)	528
MvaI	CCWGG	4 cut(s)	189, 711, 1901, 2516
MwoI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
NcoI	CCATGG	1 cut(s)	381
NdeI	CATATG	1 cut(s)	1486
NdeII	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
NlaIV	GGNNCC	9 cut(s)	6, 40, 525, 1443, 1606, 1677, 1905, 1906, 2158
NmeAIII	GCCGAG	2 cut(s)	1398, 2457
NmuCI	GTSAC	1 cut(s)	1658
NsiI	ATGCAT	1 cut(s)	2359
NspI	RCATGY	5 cut(s)	650, 814, 2185, 2357, 2361
PaeI	GCATGC	2 cut(s)	2185, 2357
PciSI	GCTCTTC	1 cut(s)	1207
PflFI	GACNNNGTC	1 cut(s)	1562
PfoI	TCCNGGA	1 cut(s)	709
PinAI	ACCGGT	1 cut(s)	2563
PleI	GAGTC	3 cut(s)	1283, 1519, 1655
PpsI	GAGTC	3 cut(s)	1283, 1519, 1655
Ppu21I	YACGTR	1 cut(s)	1514
PpuMI	RGGWCCY	2 cut(s)	672, 1904
Psp1406I	AACGTT	1 cut(s)	2067
Psp5II	RGGWCCY	2 cut(s)	672, 1904
Psp6I	CCWGG	4 cut(s)	187, 709, 1899, 2514
PspGI	CCWGG	4 cut(s)	187, 709, 1899, 2514
PspN4I	GGNNCC	9 cut(s)	6, 40, 525, 1443, 1606, 1677, 1905, 1906, 2158
PspPI	GGNCC	7 cut(s)	523, 672, 713, 1471, 1651, 1846, 1904
PspPPI	RGGWCCY	2 cut(s)	672, 1904
PsrI	GAACNNNNNNTAC	2 cut(s)	1992, 2024
PstI	CTGCAG	2 cut(s)	1255, 2128
PstNI	CAGNNNCTG	1 cut(s)	2480
PsuI	RGATCY	3 cut(s)	13, 82, 1441
PsyI	GACNNNGTC	1 cut(s)	1562
PvuII	CAGCTG	1 cut(s)	389
RsaI	GTAC	7 cut(s)	161, 235, 416, 1316, 1427, 1718, 1994
RsaNI	GTAC	7 cut(s)	160, 234, 415, 1315, 1426, 1717, 1993
RseI	CAYNNNNRTG	1 cut(s)	462
SalI	GTCGAC	1 cut(s)	602
SapI	GCTCTTC	1 cut(s)	1207
Sau3AI	GATC	9 cut(s)	13, 82, 184, 631, 847, 1399, 1441, 2302, 2535
Sau96I	GGNCC	7 cut(s)	523, 672, 713, 1471, 1651, 1846, 1904
ScaI	AGTACT	1 cut(s)	1316
SchI	GAGTC	3 cut(s)	1283, 1519, 1655
ScrFI	CCNGG	4 cut(s)	189, 711, 1901, 2516
SduI	GDGCHC	1 cut(s)	1680
SfaNI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
SfcI	CTRYAG	3 cut(s)	1251, 2124, 2668
SinI	GGWCC	5 cut(s)	672, 713, 1651, 1846, 1904
SmiMI	CAYNNNNRTG	1 cut(s)	462
SmlI	CTYRAG	4 cut(s)	9, 1189, 1841, 2247
SmoI	CTYRAG	4 cut(s)	9, 1189, 1841, 2247
SnaBI	TACGTA	1 cut(s)	1514
SpeI	ACTAGT	1 cut(s)	547
SphI	GCATGC	2 cut(s)	2185, 2357
SspMI	CTAG	7 cut(s)	243, 350, 548, 1086, 2147, 2169, 2683
StyD4I	CCNGG	4 cut(s)	187, 709, 1899, 2514
StyI	CCWWGG	4 cut(s)	381, 1474, 1705, 1720
TaaI	ACNGT	5 cut(s)	798, 1174, 1992, 2347, 2449
TaiI	ACGT	6 cut(s)	165, 1516, 1563, 1602, 2014, 2070
TaqI	TCGA	4 cut(s)	165, 603, 886, 2485
TaqII	GACCGA	2 cut(s)	1668, 1850
TatI	WGTACW	3 cut(s)	414, 1314, 1425
TauI	GCSGC	2 cut(s)	33, 39
TscAI	CASTG	4 cut(s)	58, 801, 1753, 2133
TseFI	GTSAC	1 cut(s)	1658
Tsp45I	GTSAC	1 cut(s)	1658
TspGWI	ACGGA	6 cut(s)	52, 221, 309, 355, 2010, 2117
TspRI	CASTG	4 cut(s)	58, 801, 1753, 2133
Tth111I	GACNNNGTC	1 cut(s)	1562
Vha464I	CTTAAG	1 cut(s)	1841
VpaK11BI	GGWCC	5 cut(s)	672, 713, 1651, 1846, 1904
XapI	RAATTY	2 cut(s)	661, 761
XceI	RCATGY	5 cut(s)	650, 814, 2185, 2357, 2361
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1343
XmiI	GTMKAC	2 cut(s)	603, 1596
XspI	CTAG	7 cut(s)	243, 350, 548, 1086, 2147, 2169, 2683
ZrmI	AGTACT	1 cut(s)	1316
Zsp2I	ATGCAT	1 cut(s)	2359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.