Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010697

Physical Location & Seq

Genomic Coordinates Reverse (-)

14066731 .. 14072582

Gene Structure 5852 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G28470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGGCTCCAGTTCCGGATCCGAACAGTGTTGGCGGTGGTGCGAAGCGGGACGAAGCTACGACGAAGATTCCTTCTAAGGATTCCAAGAAGAAAGATGATAAAAAGGAAGAGGATCTGTCTGAAGAGGACTTGCAACTAAAGCAGAATCTGGAGCTCTATGTTGAGAGAGTTCAGGACCCCAATCCTGAGTTACAGAAGATTGCTCTTGAAAGCATGAGGAAGGAAATTCGTGACTCAACAAGCTCCATGACATCAGTTCCAAAACCCCTCAAGTTTCTCCGCCCTCATTATGGAGTTCTTAAAGAATTTCATGCAAAAATGGCAGAGTCTGATCTCAAAAAAATGTTGGCTGATATACTTTCTGTCCTGGCACTGACCATGTCTGCTGAAGGTGAAAGGGAAAGCTTAAACTATAGGTTGAATGGATCAGAGAGTGACATCGGATCGTGGGGTCACGAGTATGTCAGGAATTTGGCTGGAGAGATTGCAAAAGAGTATACAATACGTCAGGGTGAAGAGTCCTCTATTGAAGACTTAATGGATCTTGTGCAGCAAATTGTTTCTTTTCACATGAAGCATAATGCCGAAACCGAAGCTGTTGACCTTTTAATGGATGTTGAGGATCTTGATCTCTTACTTGAGCATGTTGACAATACAAATTTCAGGAGGACGTGCAACTATCTCACGAGTGCAGCAAAGTATCTTCCAGGACCAGATGACATGTTGGTTCTAGATATTGCTTACATGATCTACATTAAGTTTGCAGAATATCCAAACGCGCTGCAAATTGCATTATTTCTTGATAACATGCAGTATGTGAAGCAAGTATTTACCTCATGTACCGATCTGGTAAAAAAGAAACAGTTCTGCTACATGATTGCACGTCATGGTATGACCTTTGAGCTTGACCAAGAGATGGTTGCAAATGACGAAGACAAAGAAGCGCTACAGGATATTGTTAACAACTCTAAGTTGAGTGAAGGATATCTGACGCTTGCTAGGGATATTGAGGTCATGGAGGCCAAGACGCCTGAAGACATCTACAAGGCTCACTTGCTTGATGGTAGGGCTAGCTCTGGTCCAAGTGTGGACTCAGCTAGGCAAAATCTATCTGCGACTTTTGTGAATGCATTTGTAAATGCTGGATTCGGCCAGGACAAATTAATGACAGTACCATCTGACTCAACCAGTGGATCGGCTGGAAACTGGCTCTTCAAAAATAAAGAACATGGAAAGACCAGTGCAGTTGCTAGTCTGGGTATGATTCAATTGTGGGATGTGGAGACCGGACTAGGTCATCTCGACAAATATTTCCATAGTAACGATAATCCCGTCGTTGCTGGAGCTCTGCTGGGAGTTGGGATTGTTAATTGTGGCATTAAGAATGATTGTGACCCTGCATTTGCCCTTCTTTCAGGTTATATTGACAATGAGGATTCATCTGTCCGAATTGGTGCTATTATGGGTCTCGGGATTGCATATGCGGGTTCCCAAAATGATCAGATAAAAATCAGGTTGTCTCCAATACTAAATGATGCCAACGCACCTCTCGATGTGATTGCATTTGCTGCACTTAGTTTGGGGATGATTTATGTTGGTTCGTGTAACGAAGAGGTTGCACAATCTATTATATTTGCTTTGATGGATCGGAGCGAGGCAGAGCTGGGTGAAGCCCTCACTCGTTTCTTGCCTCTTGGACTTGGTCTTTTGTACCTTGGCAAACAGGAAAGTGTGGAGGCTACTGCAGAAGTTTCAAAGACGTTCAATGAGAAAATCAGAAAGTATTGTGATATGACACTTCTTTCATGTGCATATGCTGGAACCGGGAATGTCCTTAAGGTCCAAGACCTTCTGGCTCAGTGTGGAGAGCATCTGGTGAAAGGTGATATCCACCAGGGTCCAGCTGTGATTGGATTAGCTATGGTTGCTATGTCTGAGGAATTGGGTCTGGATATGGAGATCCGTTCTTTGGAGCGCGTACTACAGTATGGAGAGCAGAACATTCGACGTGCAGTGCCTTTGGCTCTTGGTCTCCTATGTATATCAAACCCAAAGGTGACTGTTATGGACACTTTGAGCAGACTAAGCCATGACACAGATTCAGAAGTTGCAATGGCAGCAATTATCTCCCTTGGATTGATAGGCGCTGGAACCAACAATGCAAGGATAGCTGGCATGCTTAGAAATCTCTCCAGCTATTATTACAAGGATGCCAGCCTTCTTTTCTGTGTGCGCATCGCTCAAGGGTTTGTGCATATGGGAAAGGGTCTCTTAACTCTCAATCCTTTCCACTCCGAACGGCTCTTGCTATCGCCAACCGCACTTGCTGGTATAGTGACATTGTTGCATGCATGCCTTGACATGAAATCCATTATACTGGGGAAATATCACTATGTGCTCTACTTCCTTGTTTTGGCGATGCAGCCAAGGATGATGCTGACGGTGGATCAGAGCCTGAAGCCCATCTCGGTGCCAGTGCGGGTAGGACAAGCGGTTGATGTGGTTGGACAGGCAGGCCGACCTAAGACAATCACTGGGTTCCAAACGCACTCAACACCTGTTCTCCTTGCTGCTGGGGAGAGAGCTGAACTCGCAACCGAGAAGTACATTCCGTTGTCGCCCATATTAGAAGGTTTCGTCATATTGAAGGAAAATCCAGACTACAGAGAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0001101 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010498 GO:0014070 GO:0016020 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030163 GO:0031597 GO:0032182 GO:0032991 GO:0034515 GO:0042221 GO:0042493 GO:0043130 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051726 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PC_rep

Protein Analysis

891

Amino Acids

97.95

Weight (kDa)

5.08

Isoelectric Point (pI)

37.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN1_RPN2_N	PF17781	50 - 352	8.8e-124	RPN1 N-terminal domain
RPN1_C	PF18051	835 - 888	1e-31	26S proteasome non-ATPase regulatory subunit RPN1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20580 AT4G28470
fragaria_vesca	FvH4_4g14560
malus_domestica	MD09G1242100.v1.1 MD17G1234500.v1.1
prunus_persica	Prupe.3G134800_v2.0.a1
pyrus_communis	pycom09g16070
rosa_chinensis	RchiOBHm_Chr4g0418261
rosa_laevigata	RLG00000007882 RLG00000014797
rosa_multiflora	Rmu_co8313613.1_g000001 Rmu_sc0000032.1_g000014
rosa_roxburghii	Rroxscaffold_5G00361020 Rroxscaffold_5G00361440
rosa_rugosa	Rorug04G0151500
rosa_samantha	Rh4BG217500 Rh4CG224800 Rh4DG211700
rosa_wichuraiana	Rw4G018100 Rw6G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2234
AccB1I	GGYRCC	1 cut(s)	2470
AccB7I	CCANNNNNTGG	1 cut(s)	916
AccI	GTMKAC	1 cut(s)	497
AccII	CGCG	2 cut(s)	779, 1977
AccIII	TCCGGA	1 cut(s)	13
AciI	CCGC	7 cut(s)	33, 46, 280, 1484, 2319, 2479, 2492
AcoI	YGGCCR	1 cut(s)	1150
AcsI	RAATTY	4 cut(s)	225, 305, 469, 658
AcuI	CTGAAG	4 cut(s)	141, 408, 1053, 2477
AcyI	GRCGYC	1 cut(s)	1028
AdeI	CACNNNGTG	1 cut(s)	2395
AfaI	GTAC	5 cut(s)	841, 1173, 1712, 1980, 2606
AfeI	AGCGCT	1 cut(s)	945
AfiI	CCNNNNNNNGG	5 cut(s)	290, 610, 916, 1969, 2413
AflII	CTTAAG	1 cut(s)	1835
AflIII	ACRYGT	1 cut(s)	720
AgsI	TTSAA	8 cut(s)	209, 421, 530, 1216, 1268, 1755, 1765, 2647
AjiI	CACGTC	3 cut(s)	672, 884, 2009
AjnI	CCWGG	4 cut(s)	366, 706, 1152, 1893
Alw21I	GWGCWC	3 cut(s)	156, 1348, 2400
Alw26I	GTCTC	5 cut(s)	1277, 1472, 1524, 2036, 2273
AlwNI	CAGNNNCTG	3 cut(s)	148, 329, 2455
Ama87I	CYCGRG	1 cut(s)	1469
Aor13HI	TCCGGA	1 cut(s)	13
Aor51HI	AGCGCT	1 cut(s)	945
AoxI	GGCC	3 cut(s)	1020, 1150, 2515
ApeKI	GCWGC	7 cut(s)	550, 692, 781, 1568, 2117, 2422, 2570
ApoI	RAATTY	4 cut(s)	225, 305, 469, 658
ArsI	GACNNNNNNTTYG	2 cut(s)	1385, 1417
AseI	ATTAAT	1 cut(s)	1163
Asp700I	GAANNNNTTC	1 cut(s)	863
AspLEI	GCGC	5 cut(s)	781, 946, 1977, 2147, 2235
AspS9I	GGNCC	5 cut(s)	175, 710, 1079, 1840, 1898
AsuC2I	CCSGG	1 cut(s)	1825
AsuHPI	GGTGA	6 cut(s)	404, 524, 1679, 1888, 1895, 2068
AsuNHI	GCTAGC	1 cut(s)	1070
AvaI	CYCGRG	1 cut(s)	1469
AvaII	GGWCC	5 cut(s)	175, 710, 1079, 1840, 1898
BamHI	GGATCC	1 cut(s)	16
BanI	GGYRCC	1 cut(s)	2470
BanII	GRGCYC	2 cut(s)	156, 1348
BauI	CACGAG	2 cut(s)	455, 685
BbsI	GAAGAC	3 cut(s)	537, 939, 1041
Bbv12I	GWGCWC	3 cut(s)	156, 1348, 2400
BbvI	GCAGC	7 cut(s)	562, 704, 768, 1555, 2129, 2434, 2557
BccI	CCATC	5 cut(s)	910, 1055, 1183, 1636, 2471
BceAI	ACGGC	1 cut(s)	2315
BcgI	CGANNNNNNTGC	6 cut(s)	1541, 1575, 1598, 1632, 1985, 2019
BciT130I	CCWGG	4 cut(s)	368, 708, 1154, 1895
BclI	TGATCA	1 cut(s)	1498
BcnI	CCSGG	1 cut(s)	1825
BcoDI	GTCTC	5 cut(s)	1277, 1472, 1524, 2036, 2273
BfaI	CTAG	6 cut(s)	731, 999, 1071, 1098, 1251, 1292
BfmI	CTRYAG	5 cut(s)	412, 947, 1743, 1982, 2662
BfoI	RGCGCY	2 cut(s)	947, 2148
BfrI	CTTAAG	1 cut(s)	1835
BisI	GCNGC	7 cut(s)	551, 693, 782, 1569, 2118, 2423, 2571
BlsI	GCNGC	7 cut(s)	552, 694, 783, 1570, 2119, 2424, 2572
Bme1390I	CCNGG	5 cut(s)	368, 708, 1154, 1825, 1895
Bme18I	GGWCC	5 cut(s)	175, 710, 1079, 1840, 1898
BmeT110I	CYCGRG	1 cut(s)	1469
BmgBI	CACGTC	3 cut(s)	672, 884, 2009
BmgT120I	GGNCC	5 cut(s)	175, 710, 1079, 1840, 1898
BmiI	GGNNCC	9 cut(s)	6, 18, 177, 1489, 1822, 1899, 2152, 2472, 2540
BmrFI	CCNGG	5 cut(s)	368, 708, 1154, 1825, 1895
BmrI	ACTGGG	2 cut(s)	2387, 2544
BmsI	GCATC	6 cut(s)	1525, 1879, 2200, 2244, 2409, 2424
BmtI	GCTAGC	1 cut(s)	1074
BmuI	ACTGGG	2 cut(s)	2387, 2544
BpiI	GAAGAC	3 cut(s)	537, 939, 1041
BplI	GAGNNNNNCTC	2 cut(s)	2574, 2606
BpmI	CTGGAG	4 cut(s)	170, 498, 1362, 2176
BpuEI	CTTGAG	3 cut(s)	254, 659, 2226
BpuMI	CCSGG	1 cut(s)	1825
BsaBI	GATNNNNATC	2 cut(s)	627, 1508
BsaHI	GRCGYC	1 cut(s)	1028
BsaI	GGTCTC	4 cut(s)	1277, 1472, 2036, 2273
BsaJI	CCNNGG	4 cut(s)	1714, 1894, 2131, 2426
BsaWI	WCCGGW	2 cut(s)	13, 1286
BsaXI	ACNNNNNCTCC	2 cut(s)	2547, 2577
Bsc4I	CCNNNNNNNGG	5 cut(s)	290, 610, 916, 1969, 2413
Bse1I	ACTGG	7 cut(s)	8, 1188, 1211, 1239, 2382, 2474, 2539
Bse3DI	GCAATG	1 cut(s)	2118
Bse8I	GATNNNNATC	2 cut(s)	627, 1508
BseAI	TCCGGA	1 cut(s)	13
BseBI	CCWGG	4 cut(s)	368, 708, 1154, 1895
BseDI	CCNNGG	4 cut(s)	1714, 1894, 2131, 2426
BseGI	GGATG	5 cut(s)	619, 1282, 1590, 2215, 2436
BseJI	GATNNNNATC	2 cut(s)	627, 1508
BseLI	CCNNNNNNNGG	5 cut(s)	290, 610, 916, 1969, 2413
BseMI	GCAATG	1 cut(s)	2118
BseMII	CTCAG	4 cut(s)	177, 1107, 1871, 1926
BseNI	ACTGG	7 cut(s)	8, 1188, 1211, 1239, 2382, 2474, 2539
BseXI	GCAGC	7 cut(s)	562, 704, 768, 1555, 2129, 2434, 2557
BseYI	CCCAGC	3 cut(s)	1351, 1663, 2573
BsgI	GTGCAG	5 cut(s)	569, 711, 1263, 1554, 2031
Bsh1236I	CGCG	2 cut(s)	779, 1977
BshFI	GGCC	3 cut(s)	1022, 1152, 2517
BshNI	GGYRCC	1 cut(s)	2470
BsiHKAI	GWGCWC	3 cut(s)	156, 1348, 2400
BsiHKCI	CYCGRG	1 cut(s)	1469
BsiSI	CCGG	3 cut(s)	14, 1287, 1824
BslFI	GGGAC	1 cut(s)	62
BslI	CCNNNNNNNGG	5 cut(s)	290, 610, 916, 1969, 2413
BsmAI	GTCTC	5 cut(s)	1277, 1472, 1524, 2036, 2273
BsmFI	GGGAC	1 cut(s)	62
BsmI	GAATGC	1 cut(s)	1132
BsnI	GGCC	3 cut(s)	1022, 1152, 2517
Bso31I	GGTCTC	4 cut(s)	1277, 1472, 2036, 2273
BsoBI	CYCGRG	1 cut(s)	1469
Bsp1286I	GDGCHC	3 cut(s)	156, 1348, 2400
Bsp13I	TCCGGA	1 cut(s)	13
BspACI	CCGC	7 cut(s)	33, 46, 280, 1484, 2319, 2479, 2492
BspANI	GGCC	3 cut(s)	1022, 1152, 2517
BspCNI	CTCAG	4 cut(s)	178, 1106, 1870, 1927
BspEI	TCCGGA	1 cut(s)	13
BspFNI	CGCG	2 cut(s)	779, 1977
BspLI	GGNNCC	9 cut(s)	6, 18, 177, 1489, 1822, 1899, 2152, 2472, 2540
BspMAI	CTGCAG	1 cut(s)	1747
BspOI	GCTAGC	1 cut(s)	1074
BspQI	GCTCTTC	1 cut(s)	1217
BspT107I	GGYRCC	1 cut(s)	2470
BspTI	CTTAAG	1 cut(s)	1835
BspTNI	GGTCTC	4 cut(s)	1277, 1472, 2036, 2273
BsrDI	GCAATG	1 cut(s)	2118
BsrI	ACTGG	7 cut(s)	8, 1188, 1211, 1239, 2382, 2474, 2539
BssECI	CCNNGG	4 cut(s)	1714, 1894, 2131, 2426
BssNAI	GTATAC	1 cut(s)	498
BssNI	GRCGYC	1 cut(s)	1028
BssSI	CACGAG	2 cut(s)	455, 685
BssT1I	CCWWGG	3 cut(s)	1714, 2131, 2426
Bst1107I	GTATAC	1 cut(s)	498
Bst2BI	CACGAG	2 cut(s)	455, 685
Bst2UI	CCWGG	4 cut(s)	368, 708, 1154, 1895
Bst4CI	ACNGT	6 cut(s)	26, 864, 1171, 1986, 2062, 2443
Bst6I	CTCTTC	5 cut(s)	102, 117, 510, 1217, 1605
BstACI	GRCGYC	1 cut(s)	1028
BstAFI	CTTAAG	1 cut(s)	1835
BstAPI	GCANNNNNTGC	1 cut(s)	1568
BstC8I	GCNNGC	8 cut(s)	996, 1072, 2173, 2177, 2215, 2349, 2353, 2515
BstF5I	GGATG	5 cut(s)	619, 1282, 1590, 2215, 2436
BstFNI	CGCG	2 cut(s)	779, 1977
BstH2I	RGCGCY	2 cut(s)	947, 2148
BstHHI	GCGC	5 cut(s)	781, 946, 1977, 2147, 2235
BstMAI	GTCTC	5 cut(s)	1277, 1472, 1524, 2036, 2273
BstMWI	GCNNNNNNNGC	8 cut(s)	139, 320, 1568, 1925, 2085, 2117, 2168, 2591
BstNI	CCWGG	4 cut(s)	368, 708, 1154, 1895
BstNSI	RCATGY	6 cut(s)	647, 724, 811, 2179, 2351, 2355
BstSCI	CCNGG	5 cut(s)	366, 706, 1152, 1823, 1893
BstSFI	CTRYAG	5 cut(s)	412, 947, 1743, 1982, 2662
BstUI	CGCG	2 cut(s)	779, 1977
BstV1I	GCAGC	7 cut(s)	562, 704, 768, 1555, 2129, 2434, 2557
BstV2I	GAAGAC	3 cut(s)	537, 939, 1041
BstX2I	RGATCY	5 cut(s)	16, 112, 541, 622, 1959
BstXI	CCANNNNNNTGG	1 cut(s)	2377
BstYI	RGATCY	5 cut(s)	16, 112, 541, 622, 1959
BstZ17I	GTATAC	1 cut(s)	498
BsuRI	GGCC	3 cut(s)	1022, 1152, 2517
BtgZI	GCGATG	2 cut(s)	2221, 2432
BtrI	CACGTC	3 cut(s)	672, 884, 2009
BtsCI	GGATG	5 cut(s)	619, 1282, 1590, 2215, 2436
BtsI	GCAGTG	1 cut(s)	2019
BtsIMutI	CAGTG	8 cut(s)	31, 371, 1195, 1246, 1865, 2019, 2481, 2532
Cac8I	GCNNGC	8 cut(s)	996, 1072, 2173, 2177, 2215, 2349, 2353, 2515
CaiI	CAGNNNCTG	3 cut(s)	148, 329, 2455
CfoI	GCGC	5 cut(s)	781, 946, 1977, 2147, 2235
Cfr13I	GGNCC	5 cut(s)	175, 710, 1079, 1840, 1898
CseI	GACGC	2 cut(s)	1000, 1036
Csp6I	GTAC	5 cut(s)	840, 1172, 1711, 1979, 2605
CviQI	GTAC	5 cut(s)	840, 1172, 1711, 1979, 2605
DraIII	CACNNNGTG	1 cut(s)	2395
EaeI	YGGCCR	1 cut(s)	1150
Eam1104I	CTCTTC	5 cut(s)	102, 117, 510, 1217, 1605
EarI	CTCTTC	5 cut(s)	102, 117, 510, 1217, 1605
EciI	GGCGGA	1 cut(s)	269
Ecl136II	GAGCTC	2 cut(s)	154, 1346
Eco130I	CCWWGG	3 cut(s)	1714, 2131, 2426
Eco24I	GRGCYC	2 cut(s)	156, 1348
Eco31I	GGTCTC	4 cut(s)	1277, 1472, 2036, 2273
Eco32I	GATATC	2 cut(s)	986, 1888
Eco47I	GGWCC	5 cut(s)	175, 710, 1079, 1840, 1898
Eco47III	AGCGCT	1 cut(s)	945
Eco53kI	GAGCTC	2 cut(s)	154, 1346
Eco57I	CTGAAG	4 cut(s)	141, 408, 1053, 2477
Eco88I	CYCGRG	1 cut(s)	1469
EcoICRI	GAGCTC	2 cut(s)	154, 1346
EcoO109I	RGGNCCY	1 cut(s)	175
EcoRII	CCWGG	4 cut(s)	366, 706, 1152, 1893
EcoRV	GATATC	2 cut(s)	986, 1888
EcoT14I	CCWWGG	3 cut(s)	1714, 2131, 2426
EcoT22I	ATGCAT	2 cut(s)	1132, 2353
EcoT38I	GRGCYC	2 cut(s)	156, 1348
ErhI	CCWWGG	3 cut(s)	1714, 2131, 2426
FalI	AAGNNNNNCTT	4 cut(s)	1037, 1069, 2255, 2287
FaqI	GGGAC	1 cut(s)	62
FauI	CCCGC	3 cut(s)	39, 1477, 2472
FauNDI	CATATG	3 cut(s)	1480, 1813, 2256
FbaI	TGATCA	1 cut(s)	1498
FblI	GTMKAC	1 cut(s)	497
Fnu4HI	GCNGC	7 cut(s)	551, 693, 782, 1569, 2118, 2423, 2571
FokI	GGATG	5 cut(s)	626, 1289, 1597, 2222, 2443
FriOI	GRGCYC	2 cut(s)	156, 1348
Fsp4HI	GCNGC	7 cut(s)	551, 693, 782, 1569, 2118, 2423, 2571
FspAI	RTGCGCAY	1 cut(s)	2234
FspBI	CTAG	6 cut(s)	731, 999, 1071, 1098, 1251, 1292
FspI	TGCGCA	1 cut(s)	2234
GlaI	GCGC	5 cut(s)	780, 945, 1976, 2146, 2234
GluI	GCNGC	7 cut(s)	551, 693, 782, 1569, 2118, 2423, 2571
GsaI	CCCAGC	3 cut(s)	1355, 1667, 2577
GsuI	CTGGAG	4 cut(s)	170, 498, 1362, 2176
HaeII	RGCGCY	2 cut(s)	947, 2148
HaeIII	GGCC	3 cut(s)	1022, 1152, 2517
HapII	CCGG	3 cut(s)	14, 1287, 1824
HgaI	GACGC	2 cut(s)	1000, 1036
HhaI	GCGC	5 cut(s)	781, 946, 1977, 2147, 2235
Hin1I	GRCGYC	1 cut(s)	1028
Hin6I	GCGC	5 cut(s)	779, 944, 1975, 2145, 2233
HinP1I	GCGC	5 cut(s)	779, 944, 1975, 2145, 2233
HincII	GTYRAC	3 cut(s)	601, 649, 961
HindII	GTYRAC	3 cut(s)	601, 649, 961
HindIII	AAGCTT	1 cut(s)	403
HpaI	GTTAAC	1 cut(s)	961
HpaII	CCGG	3 cut(s)	14, 1287, 1824
HphI	GGTGA	6 cut(s)	404, 524, 1679, 1888, 1895, 2068
Hpy166II	GTNNAC	5 cut(s)	498, 601, 649, 961, 1090
Hpy8I	GTNNAC	5 cut(s)	498, 601, 649, 961, 1090
Hpy99I	CGWCG	3 cut(s)	64, 1337, 2010
HpyAV	CCTTC	9 cut(s)	81, 214, 383, 974, 1418, 1859, 2228, 2624, 2641
HpyCH4III	ACNGT	6 cut(s)	26, 864, 1171, 1986, 2062, 2443
HpyCH4IV	ACGT	5 cut(s)	505, 671, 883, 1760, 2008
HpyF10VI	GCNNNNNNNGC	8 cut(s)	139, 320, 1568, 1925, 2085, 2117, 2168, 2591
HpySE526I	ACGT	5 cut(s)	505, 671, 883, 1760, 2008
Hsp92I	GRCGYC	1 cut(s)	1028
HspAI	GCGC	5 cut(s)	779, 944, 1975, 2145, 2233
Kpn2I	TCCGGA	1 cut(s)	13
Ksp22I	TGATCA	1 cut(s)	1498
KspAI	GTTAAC	1 cut(s)	961
LguI	GCTCTTC	1 cut(s)	1217
LmnI	GCTCC	6 cut(s)	10, 151, 248, 1343, 1650, 1972
Lsp1109I	GCAGC	7 cut(s)	562, 704, 768, 1555, 2129, 2434, 2557
LweI	GCATC	6 cut(s)	1525, 1879, 2200, 2244, 2409, 2424
MaeI	CTAG	6 cut(s)	731, 999, 1071, 1098, 1251, 1292
MaeII	ACGT	5 cut(s)	505, 671, 883, 1760, 2008
MaeIII	GTNAC	9 cut(s)	189, 230, 434, 452, 1319, 1391, 1604, 2056, 2335
MfeI	CAATTG	1 cut(s)	1268
MflI	RGATCY	5 cut(s)	16, 112, 541, 622, 1959
MhlI	GDGCHC	3 cut(s)	156, 1348, 2400
MlyI	GAGTC	5 cut(s)	227, 335, 527, 1085, 1175
MmeI	TCCRAC	1 cut(s)	2485
Mph1103I	ATGCAT	2 cut(s)	1132, 2353
MroI	TCCGGA	1 cut(s)	13
MroXI	GAANNNNTTC	1 cut(s)	863
MslI	CAYNNNNRTG	2 cut(s)	459, 2468
MspA1I	CMGCKG	1 cut(s)	1904
MspCI	CTTAAG	1 cut(s)	1835
MspI	CCGG	3 cut(s)	14, 1287, 1824
MspR9I	CCNGG	5 cut(s)	368, 708, 1154, 1825, 1895
MunI	CAATTG	1 cut(s)	1268
Mva1269I	GAATGC	1 cut(s)	1132
MvaI	CCWGG	4 cut(s)	368, 708, 1154, 1895
MvnI	CGCG	2 cut(s)	779, 1977
MwoI	GCNNNNNNNGC	8 cut(s)	139, 320, 1568, 1925, 2085, 2117, 2168, 2591
NciI	CCSGG	1 cut(s)	1825
NdeI	CATATG	3 cut(s)	1480, 1813, 2256
NheI	GCTAGC	1 cut(s)	1070
NlaIV	GGNNCC	9 cut(s)	6, 18, 177, 1489, 1822, 1899, 2152, 2472, 2540
NmuCI	GTSAC	6 cut(s)	230, 434, 452, 1391, 2056, 2335
NsbI	TGCGCA	1 cut(s)	2234
NsiI	ATGCAT	2 cut(s)	1132, 2353
NspI	RCATGY	6 cut(s)	647, 724, 811, 2179, 2351, 2355
PaeI	GCATGC	3 cut(s)	2179, 2351, 2355
PciI	ACATGT	1 cut(s)	720
PciSI	GCTCTTC	1 cut(s)	1217
PcsI	WCGNNNNNNNCGW	2 cut(s)	1329, 1548
PctI	GAATGC	1 cut(s)	1132
PdmI	GAANNNNTTC	1 cut(s)	863
PfeI	GAWTC	7 cut(s)	67, 80, 145, 1146, 1264, 1436, 2099
PflFI	GACNNNGTC	3 cut(s)	379, 1293, 1701
PflMI	CCANNNNNTGG	1 cut(s)	916
PfoI	TCCNGGA	1 cut(s)	706
PkrI	GCNGC	7 cut(s)	552, 694, 783, 1570, 2119, 2424, 2572
PleI	GAGTC	5 cut(s)	227, 334, 526, 1085, 1175
PpsI	GAGTC	5 cut(s)	227, 334, 526, 1085, 1175
PpuMI	RGGWCCY	1 cut(s)	175
PscI	ACATGT	1 cut(s)	720
PshBI	ATTAAT	1 cut(s)	1163
Psp124BI	GAGCTC	2 cut(s)	156, 1348
Psp5II	RGGWCCY	1 cut(s)	175
Psp6I	CCWGG	4 cut(s)	366, 706, 1152, 1893
PspFI	CCCAGC	3 cut(s)	1351, 1663, 2573
PspGI	CCWGG	4 cut(s)	366, 706, 1152, 1893
PspN4I	GGNNCC	9 cut(s)	6, 18, 177, 1489, 1822, 1899, 2152, 2472, 2540
PspPI	GGNCC	5 cut(s)	175, 710, 1079, 1840, 1898
PspPPI	RGGWCCY	1 cut(s)	175
PstI	CTGCAG	1 cut(s)	1747
PstNI	CAGNNNCTG	3 cut(s)	148, 329, 2455
PsuI	RGATCY	5 cut(s)	16, 112, 541, 622, 1959
PsyI	GACNNNGTC	3 cut(s)	379, 1293, 1701
PvuII	CAGCTG	1 cut(s)	1904
RsaI	GTAC	5 cut(s)	841, 1173, 1712, 1980, 2606
RsaNI	GTAC	5 cut(s)	840, 1172, 1711, 1979, 2605
RseI	CAYNNNNRTG	2 cut(s)	459, 2468
SacI	GAGCTC	2 cut(s)	156, 1348
SapI	GCTCTTC	1 cut(s)	1217
SatI	GCNGC	7 cut(s)	551, 693, 782, 1569, 2118, 2423, 2571
Sau96I	GGNCC	5 cut(s)	175, 710, 1079, 1840, 1898
SchI	GAGTC	5 cut(s)	227, 335, 527, 1085, 1175
ScrFI	CCNGG	5 cut(s)	368, 708, 1154, 1825, 1895
SduI	GDGCHC	3 cut(s)	156, 1348, 2400
SfaNI	GCATC	6 cut(s)	1525, 1879, 2200, 2244, 2409, 2424
SfcI	CTRYAG	5 cut(s)	412, 947, 1743, 1982, 2662
SinI	GGWCC	5 cut(s)	175, 710, 1079, 1840, 1898
SmiMI	CAYNNNNRTG	2 cut(s)	459, 2468
SmlI	CTYRAG	4 cut(s)	269, 638, 1835, 2241
SmoI	CTYRAG	4 cut(s)	269, 638, 1835, 2241
SphI	GCATGC	3 cut(s)	2179, 2351, 2355
SsiI	CCGC	7 cut(s)	33, 46, 280, 1484, 2319, 2479, 2492
SspI	AATATT	1 cut(s)	1310
SspMI	CTAG	6 cut(s)	731, 999, 1071, 1098, 1251, 1292
SstI	GAGCTC	2 cut(s)	156, 1348
StyD4I	CCNGG	5 cut(s)	366, 706, 1152, 1823, 1893
StyI	CCWWGG	3 cut(s)	1714, 2131, 2426
TaaI	ACNGT	6 cut(s)	26, 864, 1171, 1986, 2062, 2443
TaiI	ACGT	5 cut(s)	508, 674, 886, 1763, 2011
TaqI	TCGA	3 cut(s)	1302, 1551, 2005
TatI	WGTACW	1 cut(s)	2604
TfiI	GAWTC	7 cut(s)	67, 80, 145, 1146, 1264, 1436, 2099
TscAI	CASTG	8 cut(s)	31, 378, 1195, 1246, 1865, 2019, 2481, 2539
TseFI	GTSAC	6 cut(s)	230, 434, 452, 1391, 2056, 2335
TseI	GCWGC	7 cut(s)	550, 692, 781, 1568, 2117, 2422, 2570
Tsp45I	GTSAC	6 cut(s)	230, 434, 452, 1391, 2056, 2335
TspDTI	ATGAA	5 cut(s)	299, 587, 1428, 1794, 2378
TspGWI	ACGGA	2 cut(s)	1952, 2601
TspRI	CASTG	8 cut(s)	31, 378, 1195, 1246, 1865, 2019, 2481, 2539
Tth111I	GACNNNGTC	3 cut(s)	379, 1293, 1701
Van91I	CCANNNNNTGG	1 cut(s)	916
Vha464I	CTTAAG	1 cut(s)	1835
VpaK11BI	GGWCC	5 cut(s)	175, 710, 1079, 1840, 1898
VspI	ATTAAT	1 cut(s)	1163
XapI	RAATTY	4 cut(s)	225, 305, 469, 658
XbaI	TCTAGA	1 cut(s)	730
XceI	RCATGY	6 cut(s)	647, 724, 811, 2179, 2351, 2355
XmiI	GTMKAC	1 cut(s)	497
XmnI	GAANNNNTTC	1 cut(s)	863
XspI	CTAG	6 cut(s)	731, 999, 1071, 1098, 1251, 1292
Zsp2I	ATGCAT	2 cut(s)	1132, 2353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.