Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010697

Physical Location & Seq

Genomic Coordinates Forward (+)

30938814 .. 30948462

Gene Structure 9649 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1242100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGGCACCTCAAGATCCAAACGCCCCAAGCGGAAGCGGCTCCGGCAAGCGCAATGAGGATACCGTTAAAGTCCCCGCAACGGACCCCAAGAAGAAGGACGATAAGAAGGATGAGGATTTGTCTGAGGAGGATTTGGAGTTGAAGAACCAATTAGAGTTGTACGTCGAGAGGGTTCAGGATACTGATCCTGGTGTGCAGAAGAATGCTCTAGAGAGTATGAGGCAGGAAATCCGTACCTCAACTAGCTCCATGACTTCAGTTCCAAAGCCATTAAAGTATCTTCGCCCACATTATGGAACTCTAAAAGCTTACTATGAAACAATGGCAGAATCAGAGTTGAAGAAATACCTAGCAGATATATTGTCTGTTTTGGCACTGACCATGTCAGCTGAGGGAGAACGGGAAAGCTTGAAGTACAGGTTGTTGGGTTCGGATGGTGATATTGGTTCATGGGGCCATGAATATGTCAGGAACTTAGCTGGAGAAATTGCTCAGGAATTTGCTAAGCGACAGAGTGAAGAGGCCCCAATTGAAGAATTAATGGAATTAGTGCAGCAAATTGTTGCTTTTCACATGAAGCACAATGCTGAACCTGAAGCTGTCGACCTTTTAATGGAGGTTGAAGACCTTGATCTATTAATTGAGCATGTTGATAAAACAAATTTTAAGAGGACTTGTCTCTACCTCACCAGTTCAGCAAGATACCTTCCTGGACCAGATGATGTACTAGTTTTGGATATTGCATATACTATATATATGAAATTTGAAGAATATCCAAATGCGCTTCAGATTGCACTGTTCCTTGATAACATGCAGAATATAAAGAACGTCTTTACATCATGTGATGATCTGCTTCGTAAAAAGCAATTTTGTTACATCCTTGCTCGACATGGTATTAGTTTTGAGCTTGATGATGAGATGGTTGCGGAGGATGATGACAGAGAAGCATTGCAGGATATTATCAACAATACTAAGCTAAGTGAAGGTTATCTTACTCTTGCTCGTGACATTGAGGTCATGGAGGCTAAAACTCCAGAGGACATTTACAAGGCACACTTACTTGATGGCCGGGCAAGTGCTGGTGCTAGTGTTGATTCAGCAAGACAGAACTTGGCTGCAACATTTGTTAACGCTTTTGTAAATGCTGGATTTGGTCAGGATAAGTTGATGACAGTCCCATCAGATGCCTCAAGTGGAGGCTCTTCAGGAAACTGGCTTTTCAAGAATAAAGAGCATGGGAAGACCAGTGCTGCTGCAAGTTTGGGTATGATTTTATTGTGGGATGTTGACTCTGGACTTGCTCAAATTGACAAGTACTTCCACAGCAAGGATACCCATGTTATTGCTGGTGCATTGTTAGGGGTTGGGATTGTCAATTGCAGTATCAAGAATGATTGTGATCCTGCGCTGGCACTTCTTGGTGAGTACATTGATAAAGAGGATCCATTTATCCGGATTGGTGCTATAATGGGCCTTGGGATTGCATACGCTGGTGCTCAGAATGAGCAGATACGTAGTAAGCTGACTCCAATACTTAATGATGCAAAAGCCCCCCTTGACGTGGTCGCATTTACTGCAATCTCATTGGGGTTGGTATACGTTGGTTCCTGCAATGAAGAGGTTGCTCAGGCAATCATATTTGCACTAATGGACCGAAGTGACTCGGAGTTGGGGGAACCCCTTGCTCGGTTGATTCCCCTCAGCCTTGGACTTCTGTACCTTGGGAAGCAGGAAAGCGTGGAGGCCACTGCCGAAGTTTCAAAGACATTTAATGAAAAAATAAGAAAGCATTGCGATATGACTTTGCTTTCTTGTGCTTATGCAGGAACAGGCAATGTTCTTAAGGTCCAAAACCTTCTCGGTCATTGTTCACAACATCTTGAGAAAAATGAAACTCACCAGGGACCCGCTGTTCTTGGAATTGCTATGATATCAATGGCTGAAGAATTGGGAGTTGAAATGGCGATTCGTTCGTTAGAGCATCTTTTACAATACGGAGAACAAAATATCCGTCGGGCAGTTCCGTTGGCACTTGGTCTCCTTTGCATGTCAAATCCAAAGGTCAATGTTATGGACACATTGAGCAGGCTTAGCCACGATACAGATTCAGAAGTAGCAATGGCTGCAGTGATTTCATTAGGTTTGCTAGGCGCGGGAACCAACAACGCTAGAATAGCTGGCATGCTTCGCAATCTTTCAAGTTATTACTACAAAGATGCCAGCCTTCTTTTCTGTGTGCGAATTGCTCAAGGTCTTGTACATTTGGGAAAGGGTCTGTTAACTCTAAATCCATACCATTCTGATCGCTTCTTGCTATCACCAACGGCACTTGCTGGAATCATTACCTTGCTGCATGCATGTCTTGATATGAAAGCCATCATTCTTGGGAAATATCATTACATTCTCTACTTTATTGTTTTGGCAATGCAGCCGAGAATGTTGTTGACCGTGGATGAGAACCTCAAACCACTGTCCATCCCTGTTAGAGTGGGACAAGCTGTTGATGTGGTTGGCCAGGCTGGTCGTCCCAAAACAATCACTGGTTTCCAGACCCACTCAACACCGGTTCTTCTTGCTGCGGGTGATAGAGCGGAGCTGGCTACTGAAAAGTACATCCCCCTGTCCCCGATCTTGGAAGGGTTCGTGATCCTGAAAGAGAATCCAGACTACAGGGAAGATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0001101 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010498 GO:0014070 GO:0016020 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030163 GO:0031597 GO:0032182 GO:0032991 GO:0034515 GO:0042221 GO:0042493 GO:0043130 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051726 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PC_rep

Protein Analysis

895

Amino Acids

98.28

Weight (kDa)

5.05

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPN1_RPN2_N	PF17781	51 - 353	6.3e-128	RPN1 N-terminal domain
RPN1_C	PF18051	837 - 890	2.3e-31	26S proteasome non-ATPase regulatory subunit RPN1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20580 AT4G28470
fragaria_vesca	FvH4_4g14560
malus_domestica	MD09G1242100.v1.1 MD17G1234500.v1.1
prunus_persica	Prupe.3G134800_v2.0.a1
pyrus_communis	pycom09g16070
rosa_chinensis	RchiOBHm_Chr4g0418261
rosa_laevigata	RLG00000007882 RLG00000014797
rosa_multiflora	Rmu_co8313613.1_g000001 Rmu_sc0000032.1_g000014
rosa_roxburghii	Rroxscaffold_5G00361020 Rroxscaffold_5G00361440
rosa_rugosa	Rorug04G0151500
rosa_samantha	Rh4BG217500 Rh4CG224800 Rh4DG211700
rosa_wichuraiana	Rw4G018100 Rw6G006980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1013
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	293
AccBSI	CCGCTC	1 cut(s)	2591
AccI	GTMKAC	2 cut(s)	603, 1596
AccII	CGCG	1 cut(s)	2153
AccIII	TCCGGA	1 cut(s)	1452
AciI	CCGC	8 cut(s)	30, 36, 75, 926, 1908, 2153, 2579, 2591
AclWI	GGATC	6 cut(s)	8, 179, 1394, 1436, 1449, 2641
AcoI	YGGCCR	2 cut(s)	1066, 2512
AcsI	RAATTY	3 cut(s)	497, 661, 761
AcuI	CTGAAG	5 cut(s)	240, 615, 770, 1188, 1962
AfaI	GTAC	9 cut(s)	161, 235, 416, 726, 1316, 1427, 1718, 2259, 2612
AfiI	CCNNNNNNNGG	7 cut(s)	79, 293, 613, 1327, 1561, 1686, 2632
AflII	CTTAAG	1 cut(s)	1841
AgeI	ACCGGT	1 cut(s)	2563
AhlI	ACTAGT	1 cut(s)	727
AjiI	CACGTC	1 cut(s)	1561
AjnI	CCWGG	4 cut(s)	187, 709, 1899, 2514
AloI	GAACNNNNNNTCC	4 cut(s)	1896, 1928, 1992, 2024
Alw21I	GWGCWC	1 cut(s)	1498
Alw26I	GTCTC	2 cut(s)	683, 2042
AlwI	GGATC	6 cut(s)	8, 179, 1394, 1436, 1449, 2641
Aor13HI	TCCGGA	1 cut(s)	1452
AoxI	GGCC	6 cut(s)	454, 522, 1066, 1471, 1743, 2512
ApeKI	GCWGC	8 cut(s)	553, 1115, 1250, 1253, 2123, 2350, 2428, 2576
ApoI	RAATTY	3 cut(s)	497, 661, 761
ArsI	GACNNNNNNTTYG	2 cut(s)	1996, 2028
AseI	ATTAAT	2 cut(s)	539, 638
AsiGI	ACCGGT	1 cut(s)	2563
AspLEI	GCGC	4 cut(s)	51, 784, 1408, 2153
AspS9I	GGNCC	8 cut(s)	82, 454, 523, 713, 1471, 1651, 1846, 1904
AsuC2I	CCSGG	1 cut(s)	1070
AsuHPI	GGTGA	6 cut(s)	449, 679, 1433, 1889, 2310, 2594
AvaII	GGWCC	5 cut(s)	82, 713, 1651, 1846, 1904
BaeI	ACNNNNGTAYC	4 cut(s)	1323, 1356, 1700, 1733
BalI	TGGCCA	1 cut(s)	2514
BamHI	GGATCC	1 cut(s)	1441
BanI	GGYRCC	1 cut(s)	4
BauI	CACGAG	1 cut(s)	1002
BbsI	GAAGAC	2 cut(s)	630, 1247
Bbv12I	GWGCWC	1 cut(s)	1498
BbvCI	CCTCAGC	2 cut(s)	390, 1700
BbvI	GCAGC	8 cut(s)	565, 1102, 1237, 1240, 2110, 2337, 2440, 2563
BccI	CCATC	6 cut(s)	428, 913, 1058, 1186, 2384, 2483
BceAI	ACGGC	1 cut(s)	2340
BciT130I	CCWGG	4 cut(s)	189, 711, 1901, 2516
BciVI	GTATCC	3 cut(s)	52, 172, 1324
BcnI	CCSGG	1 cut(s)	1070
BcoDI	GTCTC	2 cut(s)	683, 2042
BcuI	ACTAGT	1 cut(s)	727
BfaI	CTAG	8 cut(s)	209, 243, 350, 728, 1086, 2147, 2169, 2683
BfmI	CTRYAG	2 cut(s)	2124, 2668
BfrI	CTTAAG	1 cut(s)	1841
BfuI	GTATCC	3 cut(s)	52, 172, 1324
BisI	GCNGC	9 cut(s)	37, 554, 1116, 1251, 1254, 2124, 2351, 2429, 2577
BlpI	GCTNAGC	2 cut(s)	504, 2090
BlsI	GCNGC	9 cut(s)	38, 555, 1117, 1252, 1255, 2125, 2352, 2430, 2578
BmcAI	AGTACT	1 cut(s)	1316
Bme1390I	CCNGG	5 cut(s)	189, 711, 1070, 1901, 2516
Bme18I	GGWCC	5 cut(s)	82, 713, 1651, 1846, 1904
BmgBI	CACGTC	1 cut(s)	1561
BmgT120I	GGNCC	8 cut(s)	82, 454, 523, 713, 1471, 1651, 1846, 1904
BmrFI	CCNGG	5 cut(s)	189, 711, 1070, 1901, 2516
BmsI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
BpiI	GAAGAC	2 cut(s)	630, 1247
BpmI	CTGGAG	2 cut(s)	501, 1017
Bpu10I	CCTNAGC	4 cut(s)	390, 492, 1626, 1700
Bpu1102I	GCTNAGC	2 cut(s)	504, 2090
BpuEI	CTTGAG	3 cut(s)	1174, 1901, 2232
BpuMI	CCSGG	1 cut(s)	1070
BsaAI	YACGTR	1 cut(s)	1514
BsaBI	GATNNNNATC	2 cut(s)	183, 1398
BsaI	GGTCTC	1 cut(s)	2042
BsaJI	CCNNGG	5 cut(s)	1474, 1705, 1720, 1900, 2448
BsaWI	WCCGGW	2 cut(s)	1452, 2563
BsaXI	ACNNNNNCTCC	2 cut(s)	2022, 2052
Bsc4I	CCNNNNNNNGG	7 cut(s)	79, 293, 613, 1327, 1561, 1686, 2632
Bse118I	RCCGGY	1 cut(s)	2563
Bse1I	ACTGG	4 cut(s)	690, 1217, 1245, 2545
Bse3DI	GCAATG	7 cut(s)	58, 947, 1618, 1789, 1840, 2124, 2430
Bse8I	GATNNNNATC	2 cut(s)	183, 1398
BseAI	TCCGGA	1 cut(s)	1452
BseBI	CCWGG	4 cut(s)	189, 711, 1901, 2516
BseDI	CCNNGG	5 cut(s)	1474, 1705, 1720, 1900, 2448
BseGI	GGATG	8 cut(s)	115, 439, 876, 937, 1288, 2458, 2475, 2613
BseJI	GATNNNNATC	2 cut(s)	183, 1398
BseLI	CCNNNNNNNGG	7 cut(s)	79, 293, 613, 1327, 1561, 1686, 2632
BseMI	GCAATG	7 cut(s)	58, 947, 1618, 1789, 1840, 2124, 2430
BseMII	CTCAG	6 cut(s)	114, 381, 506, 1511, 1640, 1714
BseNI	ACTGG	4 cut(s)	690, 1217, 1245, 2545
BseRI	GAGGAG	1 cut(s)	140
BseXI	GCAGC	8 cut(s)	565, 1102, 1237, 1240, 2110, 2337, 2440, 2563
BsgI	GTGCAG	2 cut(s)	215, 572
Bsh1236I	CGCG	1 cut(s)	2153
BshFI	GGCC	6 cut(s)	456, 524, 1068, 1473, 1745, 2514
BshNI	GGYRCC	1 cut(s)	4
BshTI	ACCGGT	1 cut(s)	2563
BsiHKAI	GWGCWC	1 cut(s)	1498
BsiSI	CCGG	4 cut(s)	42, 1069, 1453, 2564
BslFI	GGGAC	6 cut(s)	56, 1160, 1917, 2505, 2511, 2608
BslI	CCNNNNNNNGG	7 cut(s)	79, 293, 613, 1327, 1561, 1686, 2632
BsmAI	GTCTC	2 cut(s)	683, 2042
BsmFI	GGGAC	6 cut(s)	56, 1160, 1917, 2505, 2511, 2608
BsmI	GAATGC	1 cut(s)	208
BsnI	GGCC	6 cut(s)	456, 524, 1068, 1473, 1745, 2514
Bso31I	GGTCTC	1 cut(s)	2042
Bsp1286I	GDGCHC	1 cut(s)	1498
Bsp13I	TCCGGA	1 cut(s)	1452
Bsp1407I	TGTACA	1 cut(s)	2257
Bsp143I	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
Bsp1720I	GCTNAGC	2 cut(s)	504, 2090
BspACI	CCGC	8 cut(s)	30, 36, 75, 926, 1908, 2153, 2579, 2591
BspANI	GGCC	6 cut(s)	456, 524, 1068, 1473, 1745, 2514
BspCNI	CTCAG	6 cut(s)	115, 382, 505, 1510, 1639, 1713
BspEI	TCCGGA	1 cut(s)	1452
BspFNI	CGCG	1 cut(s)	2153
BspMAI	CTGCAG	1 cut(s)	2128
BspPI	GGATC	6 cut(s)	8, 179, 1394, 1436, 1449, 2641
BspQI	GCTCTTC	1 cut(s)	1207
BspT107I	GGYRCC	1 cut(s)	4
BspTI	CTTAAG	1 cut(s)	1841
BspTNI	GGTCTC	1 cut(s)	2042
BsrBI	CCGCTC	1 cut(s)	2591
BsrDI	GCAATG	7 cut(s)	58, 947, 1618, 1789, 1840, 2124, 2430
BsrFI	RCCGGY	1 cut(s)	2563
BsrGI	TGTACA	1 cut(s)	2257
BsrI	ACTGG	4 cut(s)	690, 1217, 1245, 2545
BssAI	RCCGGY	1 cut(s)	2563
BssECI	CCNNGG	5 cut(s)	1474, 1705, 1720, 1900, 2448
BssMI	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
BssNAI	GTATAC	1 cut(s)	1597
BssSI	CACGAG	1 cut(s)	1002
BssT1I	CCWWGG	3 cut(s)	1474, 1705, 1720
Bst1107I	GTATAC	1 cut(s)	1597
Bst2BI	CACGAG	1 cut(s)	1002
Bst2UI	CCWGG	4 cut(s)	189, 711, 1901, 2516
Bst4CI	ACNGT	5 cut(s)	64, 798, 1174, 2449, 2472
Bst6I	CTCTTC	3 cut(s)	513, 1207, 1611
BstAFI	CTTAAG	1 cut(s)	1841
BstAPI	GCANNNNNTGC	2 cut(s)	1574, 2123
BstAUI	TGTACA	1 cut(s)	2257
BstBAI	YACGTR	1 cut(s)	1514
BstC8I	GCNNGC	8 cut(s)	47, 1410, 2087, 2179, 2183, 2221, 2355, 2598
BstDSI	CCRYGG	1 cut(s)	2448
BstF5I	GGATG	8 cut(s)	115, 439, 876, 937, 1288, 2458, 2475, 2613
BstFNI	CGCG	1 cut(s)	2153
BstHHI	GCGC	4 cut(s)	51, 784, 1408, 2153
BstKTI	GATC	9 cut(s)	16, 187, 634, 850, 1402, 1444, 2305, 2631, 2649
BstMAI	GTCTC	2 cut(s)	683, 2042
BstMBI	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
BstMWI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
BstNI	CCWGG	4 cut(s)	189, 711, 1901, 2516
BstNSI	RCATGY	6 cut(s)	650, 814, 2050, 2185, 2357, 2361
BstSCI	CCNGG	5 cut(s)	187, 709, 1068, 1899, 2514
BstSFI	CTRYAG	2 cut(s)	2124, 2668
BstSNI	TACGTA	1 cut(s)	1514
BstUI	CGCG	1 cut(s)	2153
BstV1I	GCAGC	8 cut(s)	565, 1102, 1237, 1240, 2110, 2337, 2440, 2563
BstV2I	GAAGAC	2 cut(s)	630, 1247
BstX2I	RGATCY	2 cut(s)	13, 1441
BstYI	RGATCY	2 cut(s)	13, 1441
BstZ17I	GTATAC	1 cut(s)	1597
BsuI	GTATCC	3 cut(s)	52, 172, 1324
BsuRI	GGCC	6 cut(s)	456, 524, 1068, 1473, 1745, 2514
BtgI	CCRYGG	1 cut(s)	2448
BtrI	CACGTC	1 cut(s)	1561
BtsCI	GGATG	8 cut(s)	115, 439, 876, 937, 1288, 2458, 2475, 2613
BtsI	GCAGTG	2 cut(s)	1746, 2133
BtsIMutI	CAGTG	7 cut(s)	374, 794, 1252, 1746, 2133, 2468, 2538
Cac8I	GCNNGC	8 cut(s)	47, 1410, 2087, 2179, 2183, 2221, 2355, 2598
CfoI	GCGC	4 cut(s)	51, 784, 1408, 2153
Cfr10I	RCCGGY	1 cut(s)	2563
Cfr13I	GGNCC	8 cut(s)	82, 454, 523, 713, 1471, 1651, 1846, 1904
Csp6I	GTAC	9 cut(s)	160, 234, 415, 725, 1315, 1426, 1717, 2258, 2611
CspAI	ACCGGT	1 cut(s)	2563
CviQI	GTAC	9 cut(s)	160, 234, 415, 725, 1315, 1426, 1717, 2258, 2611
DpnI	GATC	9 cut(s)	15, 186, 633, 849, 1401, 1443, 2304, 2630, 2648
DpnII	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
DrdI	GACNNNNNNGTC	1 cut(s)	1013
DseDI	GACNNNNNNGTC	1 cut(s)	1013
EaeI	YGGCCR	2 cut(s)	1066, 2512
Eam1104I	CTCTTC	3 cut(s)	513, 1207, 1611
EarI	CTCTTC	3 cut(s)	513, 1207, 1611
Eco105I	TACGTA	1 cut(s)	1514
Eco130I	CCWWGG	3 cut(s)	1474, 1705, 1720
Eco31I	GGTCTC	1 cut(s)	2042
Eco32I	GATATC	1 cut(s)	1932
Eco47I	GGWCC	5 cut(s)	82, 713, 1651, 1846, 1904
Eco57I	CTGAAG	5 cut(s)	240, 615, 770, 1188, 1962
EcoO109I	RGGNCCY	2 cut(s)	523, 1904
EcoRII	CCWGG	4 cut(s)	187, 709, 1899, 2514
EcoRV	GATATC	1 cut(s)	1932
EcoT14I	CCWWGG	3 cut(s)	1474, 1705, 1720
EcoT22I	ATGCAT	1 cut(s)	2359
ErhI	CCWWGG	3 cut(s)	1474, 1705, 1720
FalI	AAGNNNNNCTT	8 cut(s)	815, 847, 975, 1007, 1040, 1072, 1539, 1571
FaqI	GGGAC	6 cut(s)	56, 1160, 1917, 2505, 2511, 2608
FauI	CCCGC	4 cut(s)	82, 1915, 2146, 2572
FblI	GTMKAC	2 cut(s)	603, 1596
Fnu4HI	GCNGC	9 cut(s)	37, 554, 1116, 1251, 1254, 2124, 2351, 2429, 2577
FokI	GGATG	8 cut(s)	122, 446, 863, 944, 1295, 2462, 2465, 2600
Fsp4HI	GCNGC	9 cut(s)	37, 554, 1116, 1251, 1254, 2124, 2351, 2429, 2577
FspBI	CTAG	8 cut(s)	209, 243, 350, 728, 1086, 2147, 2169, 2683
GlaI	GCGC	4 cut(s)	50, 783, 1407, 2152
GluI	GCNGC	9 cut(s)	37, 554, 1116, 1251, 1254, 2124, 2351, 2429, 2577
GsuI	CTGGAG	2 cut(s)	501, 1017
HaeIII	GGCC	6 cut(s)	456, 524, 1068, 1473, 1745, 2514
HapII	CCGG	4 cut(s)	42, 1069, 1453, 2564
HhaI	GCGC	4 cut(s)	51, 784, 1408, 2153
Hin6I	GCGC	4 cut(s)	49, 782, 1406, 2151
HinP1I	GCGC	4 cut(s)	49, 782, 1406, 2151
HincII	GTYRAC	5 cut(s)	604, 1129, 1288, 2280, 2445
HindII	GTYRAC	5 cut(s)	604, 1129, 1288, 2280, 2445
HindIII	AAGCTT	2 cut(s)	306, 406
HpaI	GTTAAC	2 cut(s)	1129, 2280
HpaII	CCGG	4 cut(s)	42, 1069, 1453, 2564
HphI	GGTGA	6 cut(s)	449, 679, 1433, 1889, 2310, 2594
Hpy166II	GTNNAC	7 cut(s)	604, 1129, 1288, 1597, 1871, 2280, 2445
Hpy188I	TCNGA	9 cut(s)	124, 334, 433, 789, 1183, 1500, 1666, 2110, 2302
Hpy8I	GTNNAC	7 cut(s)	604, 1129, 1288, 1597, 1871, 2280, 2445
Hpy99I	CGWCG	2 cut(s)	167, 2016
HpyAV	CCTTC	7 cut(s)	88, 100, 716, 977, 1865, 2234, 2630
HpyCH4III	ACNGT	5 cut(s)	64, 798, 1174, 2449, 2472
HpyCH4IV	ACGT	5 cut(s)	162, 828, 1513, 1560, 1599
HpyF10VI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
HpySE526I	ACGT	5 cut(s)	162, 828, 1513, 1560, 1599
HspAI	GCGC	4 cut(s)	49, 782, 1406, 2151
KflI	GGGWCCC	1 cut(s)	1904
Kpn2I	TCCGGA	1 cut(s)	1452
KspAI	GTTAAC	2 cut(s)	1129, 2280
Kzo9I	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
LguI	GCTCTTC	1 cut(s)	1207
LmnI	GCTCC	3 cut(s)	44, 251, 2593
Lsp1109I	GCAGC	8 cut(s)	565, 1102, 1237, 1240, 2110, 2337, 2440, 2563
LweI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
MaeI	CTAG	8 cut(s)	209, 243, 350, 728, 1086, 2147, 2169, 2683
MaeII	ACGT	5 cut(s)	162, 828, 1513, 1560, 1599
MaeIII	GTNAC	3 cut(s)	872, 1004, 1658
MalI	GATC	9 cut(s)	15, 186, 633, 849, 1401, 1443, 2304, 2630, 2648
MbiI	CCGCTC	1 cut(s)	2591
MboI	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
MfeI	CAATTG	2 cut(s)	528, 1375
MflI	RGATCY	2 cut(s)	13, 1441
MhlI	GDGCHC	1 cut(s)	1498
MlsI	TGGCCA	1 cut(s)	2514
MluNI	TGGCCA	1 cut(s)	2514
MlyI	GAGTC	3 cut(s)	1283, 1519, 1655
Mox20I	TGGCCA	1 cut(s)	2514
Mph1103I	ATGCAT	1 cut(s)	2359
MroI	TCCGGA	1 cut(s)	1452
MscI	TGGCCA	1 cut(s)	2514
MslI	CAYNNNNRTG	1 cut(s)	462
Msp20I	TGGCCA	1 cut(s)	2514
MspA1I	CMGCKG	2 cut(s)	389, 1910
MspCI	CTTAAG	1 cut(s)	1841
MspI	CCGG	4 cut(s)	42, 1069, 1453, 2564
MspR9I	CCNGG	5 cut(s)	189, 711, 1070, 1901, 2516
MunI	CAATTG	2 cut(s)	528, 1375
Mva1269I	GAATGC	1 cut(s)	208
MvaI	CCWGG	4 cut(s)	189, 711, 1901, 2516
MvnI	CGCG	1 cut(s)	2153
MwoI	GCNNNNNNNGC	8 cut(s)	36, 42, 1574, 2091, 2123, 2174, 2187, 2597
NciI	CCSGG	1 cut(s)	1070
NdeII	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
NmeAIII	GCCGAG	1 cut(s)	2457
NmuCI	GTSAC	2 cut(s)	1004, 1658
NsiI	ATGCAT	1 cut(s)	2359
NspI	RCATGY	6 cut(s)	650, 814, 2050, 2185, 2357, 2361
PaeI	GCATGC	2 cut(s)	2185, 2357
PciSI	GCTCTTC	1 cut(s)	1207
PctI	GAATGC	1 cut(s)	208
PfeI	GAWTC	7 cut(s)	329, 1094, 1693, 1966, 2105, 2337, 2659
PflFI	GACNNNGTC	2 cut(s)	382, 1562
PflMI	CCANNNNNTGG	1 cut(s)	293
PfoI	TCCNGGA	1 cut(s)	709
PinAI	ACCGGT	1 cut(s)	2563
PkrI	GCNGC	9 cut(s)	38, 555, 1117, 1252, 1255, 2125, 2352, 2430, 2578
PleI	GAGTC	3 cut(s)	1283, 1519, 1655
PpsI	GAGTC	3 cut(s)	1283, 1519, 1655
Ppu21I	YACGTR	1 cut(s)	1514
PpuMI	RGGWCCY	1 cut(s)	1904
PshBI	ATTAAT	2 cut(s)	539, 638
Psp5II	RGGWCCY	1 cut(s)	1904
Psp6I	CCWGG	4 cut(s)	187, 709, 1899, 2514
PspGI	CCWGG	4 cut(s)	187, 709, 1899, 2514
PspPI	GGNCC	8 cut(s)	82, 454, 523, 713, 1471, 1651, 1846, 1904
PspPPI	RGGWCCY	1 cut(s)	1904
PstI	CTGCAG	1 cut(s)	2128
PsuI	RGATCY	2 cut(s)	13, 1441
PsyI	GACNNNGTC	2 cut(s)	382, 1562
PvuII	CAGCTG	1 cut(s)	389
RsaI	GTAC	9 cut(s)	161, 235, 416, 726, 1316, 1427, 1718, 2259, 2612
RsaNI	GTAC	9 cut(s)	160, 234, 415, 725, 1315, 1426, 1717, 2258, 2611
RseI	CAYNNNNRTG	1 cut(s)	462
SalI	GTCGAC	1 cut(s)	602
SapI	GCTCTTC	1 cut(s)	1207
SatI	GCNGC	9 cut(s)	37, 554, 1116, 1251, 1254, 2124, 2351, 2429, 2577
Sau3AI	GATC	9 cut(s)	13, 184, 631, 847, 1399, 1441, 2302, 2628, 2646
Sau96I	GGNCC	8 cut(s)	82, 454, 523, 713, 1471, 1651, 1846, 1904
ScaI	AGTACT	1 cut(s)	1316
SchI	GAGTC	3 cut(s)	1283, 1519, 1655
ScrFI	CCNGG	5 cut(s)	189, 711, 1070, 1901, 2516
SduI	GDGCHC	1 cut(s)	1498
SfaNI	GCATC	4 cut(s)	1174, 1531, 1990, 2206
SfcI	CTRYAG	2 cut(s)	2124, 2668
SinI	GGWCC	5 cut(s)	82, 713, 1651, 1846, 1904
SmiMI	CAYNNNNRTG	1 cut(s)	462
SmlI	CTYRAG	5 cut(s)	9, 1189, 1841, 1880, 2247
SmoI	CTYRAG	5 cut(s)	9, 1189, 1841, 1880, 2247
SnaBI	TACGTA	1 cut(s)	1514
SpeI	ACTAGT	1 cut(s)	727
SphI	GCATGC	2 cut(s)	2185, 2357
SsiI	CCGC	8 cut(s)	30, 36, 75, 926, 1908, 2153, 2579, 2591
SspMI	CTAG	8 cut(s)	209, 243, 350, 728, 1086, 2147, 2169, 2683
StyD4I	CCNGG	5 cut(s)	187, 709, 1068, 1899, 2514
StyI	CCWWGG	3 cut(s)	1474, 1705, 1720
TaaI	ACNGT	5 cut(s)	64, 798, 1174, 2449, 2472
TaiI	ACGT	5 cut(s)	165, 831, 1516, 1563, 1602
TaqI	TCGA	3 cut(s)	165, 603, 886
TaqII	GACCGA	2 cut(s)	1668, 1850
TatI	WGTACW	6 cut(s)	414, 724, 1314, 1425, 2257, 2610
TauI	GCSGC	1 cut(s)	39
TfiI	GAWTC	7 cut(s)	329, 1094, 1693, 1966, 2105, 2337, 2659
TscAI	CASTG	7 cut(s)	381, 801, 1252, 1753, 2133, 2475, 2545
TseFI	GTSAC	2 cut(s)	1004, 1658
TseI	GCWGC	8 cut(s)	553, 1115, 1250, 1253, 2123, 2350, 2428, 2576
Tsp45I	GTSAC	2 cut(s)	1004, 1658
TspGWI	ACGGA	5 cut(s)	95, 221, 2000, 2010, 2013
TspRI	CASTG	7 cut(s)	381, 801, 1252, 1753, 2133, 2475, 2545
Tth111I	GACNNNGTC	2 cut(s)	382, 1562
Van91I	CCANNNNNTGG	1 cut(s)	293
Vha464I	CTTAAG	1 cut(s)	1841
VpaK11BI	GGWCC	5 cut(s)	82, 713, 1651, 1846, 1904
VspI	ATTAAT	2 cut(s)	539, 638
XapI	RAATTY	3 cut(s)	497, 661, 761
XbaI	TCTAGA	1 cut(s)	208
XceI	RCATGY	6 cut(s)	650, 814, 2050, 2185, 2357, 2361
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1343
XmiI	GTMKAC	2 cut(s)	603, 1596
XspI	CTAG	8 cut(s)	209, 243, 350, 728, 1086, 2147, 2169, 2683
ZrmI	AGTACT	1 cut(s)	1316
Zsp2I	ATGCAT	1 cut(s)	2359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.