AT2G21350

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010962
Physical Location & Seq
Reverse (-)
9136203 .. 9137284
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21350.1

Sequence Viewer

Length: 669 bp
ATGGCGACTGCAAAATCATCGACTTTGACTAACTTGATCCACCATCTTCTCCGACATCGCAAACCAACATCTCCTGTCTGTCTGTTCCTCAGACCCTTTTCCGTGTCTCTATTAGGCCGTAAATCCAGTCATCATGCGTGGATCTCCTCTTCTTTACCTCTTCCACGTCCTTATATCAGCTTCTCTCCTCTCCTCGTACCTAAATCCTTCTCTTCTGTTGATGAATCAGAACAAGACGAAGACAACGTGTCCTTAGTAGCCTCTCTTAACGAGATTAACGATGATGGCCAAGAAGATGGGTCGGAACTTACAATGGTGTCCATGAGAGAAAACCGAAGAAGCTCGGCTTTGGAGTTGTCTGCGAAAGAGAAGAGAAAGCTTGCTTCTTACGCACATCATTTGGGTGACAAACTGAAATCTCAACTTGTAGGCAAGTCTGGCGTTACGGATTCAGTCGTCTTGTCCTTCGTAGAGACGCTGGAGAAGAACGAGCTCTTAAAGGTGAAAATACACAGAACATGCCCTGGAGAGCTAGAGGACATGATTCTACGTTTGGAGGAAGCCACTGGTTCAGTATCAGTCGGGCAAATTGCGCGGACCGTGATACTTTATCGTCCTAGTCCCACCAAATTGAAAGCCGACGAGGACAAAAAACGGAATGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

222

Amino Acids

24.71

Weight (kDa)

7.82

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 119 - 205 2.1e-18 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 595
AciI CCGC 1 cut(s) 595
AclWI GGATC 2 cut(s) 31, 149
AcoI YGGCCR 1 cut(s) 286
AfaI GTAC 1 cut(s) 198
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 1 cut(s) 634
AhdI GACNNNNNGTC 1 cut(s) 247
AjiI CACGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 523
AluBI AGCT 5 cut(s) 180, 342, 379, 493, 532
AluI AGCT 5 cut(s) 180, 342, 379, 493, 532
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 2 cut(s) 111, 467
AlwI GGATC 2 cut(s) 31, 149
AoxI GGCC 2 cut(s) 115, 286
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 2 cut(s) 416, 514
AvaII GGWCC 1 cut(s) 597
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 495
BccI CCATC 3 cut(s) 51, 278, 290
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 111, 467
BfaI CTAG 2 cut(s) 533, 618
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 1 cut(s) 597
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 597
BmrFI CCNGG 1 cut(s) 525
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 2 cut(s) 500, 546
BsaJI CCNNGG 1 cut(s) 523
Bse1I ACTGG 2 cut(s) 126, 571
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 523
BseMII CTCAG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 126, 571
BseRI GAGGAG 3 cut(s) 136, 177, 182
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 2 cut(s) 117, 288
BsiHKAI GWGCWC 1 cut(s) 495
BslFI GGGAC 1 cut(s) 606
BsmAI GTCTC 2 cut(s) 111, 467
BsmBI CGTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 2 cut(s) 117, 288
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 2 cut(s) 36, 141
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 117, 288
BspCNI CTCAG 1 cut(s) 102
BspFNI CGCG 1 cut(s) 595
BspPI GGATC 2 cut(s) 31, 149
BsrI ACTGG 2 cut(s) 126, 571
BssECI CCNNGG 1 cut(s) 523
BssMI GATC 2 cut(s) 36, 141
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 5 cut(s) 154, 165, 217, 365, 657
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 2 cut(s) 89, 253
BstFNI CGCG 1 cut(s) 595
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 2 cut(s) 39, 144
BstMAI GTCTC 2 cut(s) 111, 467
BstMBI GATC 2 cut(s) 36, 141
BstMWI GCNNNNNNNGC 3 cut(s) 389, 438, 592
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 1 cut(s) 522
BstSCI CCNGG 1 cut(s) 523
BstUI CGCG 1 cut(s) 595
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 141
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 141
BsuRI GGCC 2 cut(s) 117, 288
BtgZI GCGATG 1 cut(s) 41
BtrI CACGTC 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 597
CpoI CGGWCCG 1 cut(s) 597
CseI GACGC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 197
CspI CGGWCCG 1 cut(s) 597
CviAII CATG 4 cut(s) 134, 322, 519, 541
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 2 cut(s) 89, 253
DpnI GATC 2 cut(s) 38, 143
DpnII GATC 2 cut(s) 36, 141
DriI GACNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 5 cut(s) 154, 165, 217, 365, 657
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 5 cut(s) 154, 165, 217, 365, 657
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 597
Eco53kI GAGCTC 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 4 cut(s) 137, 325, 522, 544
FaiI YATR 5 cut(s) 135, 174, 323, 520, 542
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FaqI GGGAC 1 cut(s) 606
FatI CATG 4 cut(s) 133, 321, 518, 540
FriOI GRGCYC 1 cut(s) 495
FspBI CTAG 2 cut(s) 533, 618
GlaI GCGC 1 cut(s) 594
GsuI CTGGAG 2 cut(s) 500, 546
HaeIII GGCC 2 cut(s) 117, 288
HgaI GACGC 1 cut(s) 484
HhaI GCGC 1 cut(s) 595
Hin1II CATG 4 cut(s) 137, 325, 522, 544
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 224, 449, 544
HphI GGTGA 2 cut(s) 416, 514
Hpy188I TCNGA 4 cut(s) 53, 92, 229, 304
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 2 cut(s) 217, 475
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 3 cut(s) 166, 246, 550
HpyCH4V TGCA 1 cut(s) 11
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 438, 592
HpyF3I CTNAG 2 cut(s) 89, 253
HpySE526I ACGT 3 cut(s) 166, 246, 550
Hsp92II CATG 4 cut(s) 137, 325, 522, 544
HspAI GCGC 1 cut(s) 593
Kzo9I GATC 2 cut(s) 36, 141
LpnPI CCDG 7 cut(s) 87, 139, 423, 464, 510, 537, 552
MaeI CTAG 2 cut(s) 533, 618
MaeII ACGT 3 cut(s) 166, 246, 550
MaeIII GTNAC 2 cut(s) 404, 442
MalI GATC 2 cut(s) 38, 143
MboI GATC 2 cut(s) 36, 141
MboII GAAGA 9 cut(s) 38, 141, 152, 204, 251, 305, 348, 382, 496
MflI RGATCY 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 495
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 2 cut(s) 588, 629
MluNI TGGCCA 1 cut(s) 288
MmeI TCCRAC 2 cut(s) 76, 282
MnlI CCTC 9 cut(s) 98, 157, 168, 198, 203, 271, 529, 550, 637
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 3 cut(s) 267, 276, 497
MslI CAYNNNNRTG 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 3 cut(s) 389, 438, 592
NdeII GATC 2 cut(s) 36, 141
NlaIII CATG 4 cut(s) 137, 325, 522, 544
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 522
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 276
PfeI GAWTC 3 cut(s) 224, 449, 544
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspPI GGNCC 1 cut(s) 597
PsuI RGATCY 1 cut(s) 141
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 402
Rsr2I CGGWCCG 1 cut(s) 597
RsrII CGGWCCG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 3 cut(s) 267, 276, 497
Sau3AI GATC 2 cut(s) 36, 141
Sau96I GGNCC 1 cut(s) 597
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 495
SinI GGWCC 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 588, 629
SsiI CCGC 1 cut(s) 595
SspMI CTAG 2 cut(s) 533, 618
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 523
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 3 cut(s) 169, 249, 553
TaqI TCGA 1 cut(s) 20
TasI AATT 2 cut(s) 588, 629
TfiI GAWTC 3 cut(s) 224, 449, 544
Tru1I TTAA 3 cut(s) 267, 276, 497
Tru9I TTAA 3 cut(s) 267, 276, 497
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 2 cut(s) 91, 461
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 597
XceI RCATGY 1 cut(s) 522
XspI CTAG 2 cut(s) 533, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.