FvH4_2g10110

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010962
Physical Location & Seq
Forward (+)
8939236 .. 8941387
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10110.t1

Sequence Viewer

Length: 774 bp
ATGGCGGCAGCAGTAGCAGCAGCAGCTGTTGGAGTCCCAACCCTGCTCCACCGCCTCGTCCACCACCCACCACCCCGTTCCTTTTTCCTCATCTCCAGCACCGCCATATCCAAACAACCACAACTACGACGCCTCTTGTGCCACTCCCTACCTTCCACCAAAGCCTCTTTGCAACAACAACAACCAACCCAGCAACTTGATGAGAATGCCCAGATGGGTTTGGACTCTGAAAATGGTGAGGAAGGTGAGACTGAAGCTGGGAAACTGGATACTAACGACGCTGCACGAATGAACTTAGACCCTTCTACGGTTTTGAAGAGAAACCGGGTTGACCCGGTGGACCTTCCGACACTTACGGTGAGAGAAAAGAAGGAGTTGGCGTCGTATGCTAACAGCTTGGGGAAGAAGCTCAAGTCTCAGTTGGTGGGCAAGTCTGGTGTTACGCCCAACGTTGCTGCTGCTTTTGTTGAGAATCTTGAATCTAATGAGCTTCTTAAGGTTAAAATACACGGGACATGTCCAGAGGAGCTAGATGATACAGTGAAAGAACTGGAGGCAGCAACTGGTTCAGTTGTTGTTGGTCGGATTGGTAGAACAGTGATTCTTTACAGGCCAAGTCTCACCAAGCTGAAAGCAGAGGAGAAAAAAGAGCAGATGCGTAAGATTTTTTTAAAACGAAAGCAGAGATATTCAAGACCAACATCTATGGAATTTCAGAAAAAAGGAAGACCGAGGATGTTGGTGCGTGATAGTCGTGGAAGACCCAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

258

Amino Acids

28.46

Weight (kDa)

9.87

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 118 - 204 6.5e-20 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 52, 102
AclI AACGTT 1 cut(s) 450
AcsI RAATTY 1 cut(s) 710
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 2 cut(s) 130, 380
AfiI CCNNNNNNNGG 1 cut(s) 307
AflII CTTAAG 1 cut(s) 494
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 3 cut(s) 316, 479, 693
AhdI GACNNNNNGTC 1 cut(s) 767
AluBI AGCT 7 cut(s) 26, 257, 396, 409, 490, 529, 628
AluI AGCT 7 cut(s) 26, 257, 396, 409, 490, 529, 628
Alw26I GTCTC 3 cut(s) 242, 420, 623
AlwNI CAGNNNCTG 2 cut(s) 26, 563
AoxI GGCC 1 cut(s) 611
ApeKI GCWGC 8 cut(s) 8, 17, 20, 23, 281, 455, 458, 557
ApoI RAATTY 1 cut(s) 710
AspS9I GGNCC 1 cut(s) 340
AsuC2I CCSGG 2 cut(s) 326, 335
AsuHPI GGTGA 4 cut(s) 248, 257, 370, 613
AvaII GGWCC 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 733, 766
BbvI GCAGC 8 cut(s) 20, 29, 32, 35, 268, 442, 445, 569
BccI CCATC 1 cut(s) 208
BciVI GTATCC 1 cut(s) 262
BcnI CCSGG 2 cut(s) 326, 335
BcoDI GTCTC 3 cut(s) 242, 420, 623
BfaI CTAG 2 cut(s) 530, 772
BfrI CTTAAG 1 cut(s) 494
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 9 cut(s) 6, 9, 18, 21, 24, 282, 456, 459, 558
BlsI GCNGC 9 cut(s) 7, 10, 19, 22, 25, 283, 457, 460, 559
Bme1390I CCNGG 2 cut(s) 326, 335
Bme18I GGWCC 1 cut(s) 340
BmeRI GACNNNNNGTC 1 cut(s) 767
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 326, 335
BmsI GCATC 1 cut(s) 645
BpiI GAAGAC 2 cut(s) 733, 766
BpmI CTGGAG 2 cut(s) 79, 572
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 326, 335
BsaHI GRCGYC 2 cut(s) 130, 380
BsaJI CCNNGG 2 cut(s) 731, 765
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 3 cut(s) 270, 555, 568
BseDI CCNNGG 2 cut(s) 731, 765
BseGI GGATG 1 cut(s) 741
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 3 cut(s) 270, 555, 568
BseRI GAGGAG 2 cut(s) 539, 653
BseXI GCAGC 8 cut(s) 20, 29, 32, 35, 268, 442, 445, 569
BseYI CCCAGC 2 cut(s) 189, 257
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 1 cut(s) 613
BsiSI CCGG 2 cut(s) 325, 335
BslFI GGGAC 2 cut(s) 20, 526
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 3 cut(s) 242, 420, 623
BsmFI GGGAC 2 cut(s) 20, 526
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 1 cut(s) 613
BspACI CCGC 3 cut(s) 5, 52, 102
BspANI GGCC 1 cut(s) 613
BspCNI CTCAG 1 cut(s) 430
BspTI CTTAAG 1 cut(s) 494
BsrI ACTGG 3 cut(s) 270, 555, 568
BssECI CCNNGG 2 cut(s) 731, 765
BssNI GRCGYC 2 cut(s) 130, 380
BssT1I CCWWGG 1 cut(s) 765
Bst4CI ACNGT 4 cut(s) 310, 358, 541, 598
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 130, 380
BstAFI CTTAAG 1 cut(s) 494
BstDEI CTNAG 2 cut(s) 295, 417
BstF5I GGATG 1 cut(s) 741
BstMAI GTCTC 3 cut(s) 242, 420, 623
BstMWI GCNNNNNNNGC 5 cut(s) 14, 17, 23, 138, 386
BstNSI RCATGY 1 cut(s) 519
BstSCI CCNGG 2 cut(s) 324, 333
BstV1I GCAGC 8 cut(s) 20, 29, 32, 35, 268, 442, 445, 569
BstV2I GAAGAC 2 cut(s) 733, 766
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 613
BtsCI GGATG 1 cut(s) 741
BtsIMutI CAGTG 2 cut(s) 546, 603
CaiI CAGNNNCTG 2 cut(s) 26, 563
Cfr13I GGNCC 1 cut(s) 340
CseI GACGC 3 cut(s) 138, 287, 369
CviAII CATG 1 cut(s) 516
CviJI RGCY 9 cut(s) 26, 164, 257, 396, 409, 490, 529, 613, 628
CviKI_1 RGCY 9 cut(s) 26, 164, 257, 396, 409, 490, 529, 613, 628
DdeI CTNAG 2 cut(s) 295, 417
DraI TTTAAA 1 cut(s) 672
DriI GACNNNNNGTC 1 cut(s) 767
Eam1104I CTCTTC 1 cut(s) 311
Eam1105I GACNNNNNGTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 1 cut(s) 519
FaiI YATR 4 cut(s) 107, 387, 517, 707
FaqI GGGAC 2 cut(s) 20, 526
FatI CATG 1 cut(s) 515
Fnu4HI GCNGC 9 cut(s) 6, 9, 18, 21, 24, 282, 456, 459, 558
FokI GGATG 1 cut(s) 748
Fsp4HI GCNGC 9 cut(s) 6, 9, 18, 21, 24, 282, 456, 459, 558
FspBI CTAG 2 cut(s) 530, 772
GluI GCNGC 9 cut(s) 6, 9, 18, 21, 24, 282, 456, 459, 558
GsaI CCCAGC 2 cut(s) 193, 261
GsuI CTGGAG 2 cut(s) 79, 572
HaeIII GGCC 1 cut(s) 613
HapII CCGG 2 cut(s) 325, 335
HgaI GACGC 3 cut(s) 138, 287, 369
Hin1I GRCGYC 2 cut(s) 130, 380
Hin1II CATG 1 cut(s) 519
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 5 cut(s) 33, 224, 472, 479, 601
HpaII CCGG 2 cut(s) 325, 335
HphI GGTGA 4 cut(s) 248, 257, 370, 613
Hpy166II GTNNAC 3 cut(s) 61, 331, 340
Hpy188I TCNGA 4 cut(s) 229, 348, 585, 717
Hpy188III TCNNGA 3 cut(s) 476, 521, 693
Hpy8I GTNNAC 3 cut(s) 61, 331, 340
Hpy99I CGWCG 3 cut(s) 132, 281, 385
HpyAV CCTTC 5 cut(s) 162, 236, 312, 353, 364
HpyCH4III ACNGT 4 cut(s) 310, 358, 541, 598
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 2 cut(s) 172, 284
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 17, 23, 138, 386
HpyF3I CTNAG 2 cut(s) 295, 417
HpySE526I ACGT 1 cut(s) 450
Hsp92I GRCGYC 2 cut(s) 130, 380
Hsp92II CATG 1 cut(s) 519
LmnI GCTCC 2 cut(s) 51, 526
Lsp1109I GCAGC 8 cut(s) 20, 29, 32, 35, 268, 442, 445, 569
LweI GCATC 1 cut(s) 645
MaeI CTAG 2 cut(s) 530, 772
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 439
MboII GAAGA 4 cut(s) 328, 415, 738, 771
MluCI AATT 1 cut(s) 710
MlyI GAGTC 2 cut(s) 42, 218
MmeI TCCRAC 3 cut(s) 10, 371, 563
MnlI CCTC 9 cut(s) 65, 98, 143, 175, 232, 517, 547, 631, 726
MseI TTAA 3 cut(s) 495, 501, 671
MspA1I CMGCKG 1 cut(s) 26
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 325, 335
MspR9I CCNGG 2 cut(s) 326, 335
Mva1269I GAATGC 1 cut(s) 211
MwoI GCNNNNNNNGC 5 cut(s) 14, 17, 23, 138, 386
NciI CCSGG 2 cut(s) 326, 335
NlaIII CATG 1 cut(s) 519
NspI RCATGY 1 cut(s) 519
PciI ACATGT 1 cut(s) 515
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 3 cut(s) 472, 479, 601
PkrI GCNGC 9 cut(s) 7, 10, 19, 22, 25, 283, 457, 460, 559
PleI GAGTC 2 cut(s) 41, 218
PpsI GAGTC 2 cut(s) 41, 218
PscI ACATGT 1 cut(s) 515
Psp1406I AACGTT 1 cut(s) 450
PspFI CCCAGC 2 cut(s) 189, 257
PspPI GGNCC 1 cut(s) 340
PstNI CAGNNNCTG 2 cut(s) 26, 563
PvuII CAGCTG 1 cut(s) 26
SaqAI TTAA 3 cut(s) 495, 501, 671
SatI GCNGC 9 cut(s) 6, 9, 18, 21, 24, 282, 456, 459, 558
Sau96I GGNCC 1 cut(s) 340
SchI GAGTC 2 cut(s) 42, 218
ScrFI CCNGG 2 cut(s) 326, 335
SfaNI GCATC 1 cut(s) 645
SinI GGWCC 1 cut(s) 340
SmlI CTYRAG 2 cut(s) 410, 494
SmoI CTYRAG 2 cut(s) 410, 494
Sse9I AATT 1 cut(s) 710
SsiI CCGC 3 cut(s) 5, 52, 102
SspMI CTAG 2 cut(s) 530, 772
StyD4I CCNGG 2 cut(s) 324, 333
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 4 cut(s) 310, 358, 541, 598
TaiI ACGT 1 cut(s) 453
TaqII GACCGA 1 cut(s) 745
TasI AATT 1 cut(s) 710
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 472, 479, 601
Tru1I TTAA 3 cut(s) 495, 501, 671
Tru9I TTAA 3 cut(s) 495, 501, 671
TscAI CASTG 2 cut(s) 546, 603
TseI GCWGC 8 cut(s) 8, 17, 20, 23, 281, 455, 458, 557
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 2 cut(s) 546, 603
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 1 cut(s) 710
XceI RCATGY 1 cut(s) 519
XspI CTAG 2 cut(s) 530, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.