Rmu_ssc0000388.1_g000005

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000388.1
OG0010962
Physical Location & Seq
Forward (+)
13391 .. 15592
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000388.1_g000005.1.cds

Sequence Viewer

Length: 870 bp
atggcggcagtagcagcagcggtagttggagctccaacccggctccaccgcctcctccaccacccaccaccctcctttttcctcaaaccccttctcatctccaccaccgccgtatccaaacaaccccaaatgcgacgccccaagcttatctccacttgtcttcctttcatcccctcacgcctcttccgccactcccttcccgccatcaaagcctctctgcaagtagaacaaccaacccaagaactcgatgaggaagctgagaatgaagatgagaacttggactcttcttcttcttcttcttctgtgctcggaagtctagacgatgaaaatggtggggaaggtgagactgaagctgggaaattggataccaacgacgctgcccgaatgaatttggacccgtctaccgttttgaagaggaaccgggtcgacccggtcgaccttccgacgcttacggtgagagaaaagaaggaattggcgtcgtatgctaacagcttggggaagaagctcaagtcccagttggtgggcaagtctggagttacggccaatgtggctgcttcttttgtagagaatcttgaatctaatgagcttctcaaggttaaaatacatgggacatgtccagaggagctagatgatacggtgaagcaattggaggctgcaactggttcagttgttgtcggtcggattggtagaacagtgattctttacaggccaagtctcaccaagctaaaagctgaggagaaaaaagagcagatgcggaagatttttttgaagcgaaagcagagatattcacgaccatcatctatggaatttcagaaaaaaggaagaccgaggatgtttgtgcgtgacagccgtggaagacccaaggtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

289

Amino Acids

31.96

Weight (kDa)

9.62

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 520
AccI GTMKAC 3 cut(s) 401, 426, 435
AciI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 754
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 2 cut(s) 388, 806
AcuI CTGAAG 1 cut(s) 369
AcyI GRCGYC 2 cut(s) 136, 476
AfiI CCNNNNNNNGG 1 cut(s) 520
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 3 cut(s) 412, 575, 769
AhdI GACNNNNNGTC 1 cut(s) 863
Alw21I GWGCWC 2 cut(s) 34, 309
Alw26I GTCTC 2 cut(s) 338, 719
AoxI GGCC 2 cut(s) 540, 707
ApeKI GCWGC 5 cut(s) 14, 17, 377, 551, 653
ApoI RAATTY 2 cut(s) 388, 806
AspS9I GGNCC 1 cut(s) 394
AsuC2I CCSGG 3 cut(s) 40, 422, 431
AsuHPI GGTGA 4 cut(s) 353, 466, 649, 709
AvaII GGWCC 1 cut(s) 394
BaeI ACNNNNGTAYC 2 cut(s) 96, 129
BanII GRGCYC 1 cut(s) 34
BbsI GAAGAC 3 cut(s) 152, 829, 862
Bbv12I GWGCWC 2 cut(s) 34, 309
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 5 cut(s) 26, 29, 364, 538, 640
BccI CCATC 2 cut(s) 212, 802
BceAI ACGGC 3 cut(s) 95, 555, 834
BciVI GTATCC 2 cut(s) 124, 358
BcnI CCSGG 3 cut(s) 40, 422, 431
BcoDI GTCTC 2 cut(s) 338, 719
BfaI CTAG 3 cut(s) 317, 626, 868
BfuI GTATCC 2 cut(s) 124, 358
BglI GCCNNNNNGGC 1 cut(s) 548
BisI GCNGC 6 cut(s) 6, 15, 18, 378, 552, 654
BlsI GCNGC 6 cut(s) 7, 16, 19, 379, 553, 655
Bme1390I CCNGG 3 cut(s) 40, 422, 431
Bme18I GGWCC 1 cut(s) 394
BmeRI GACNNNNNGTC 1 cut(s) 863
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 3 cut(s) 44, 396, 419
BmrFI CCNGG 3 cut(s) 40, 422, 431
BmrI ACTGGG 1 cut(s) 508
BmsI GCATC 1 cut(s) 741
BmuI ACTGGG 1 cut(s) 508
BpiI GAAGAC 3 cut(s) 152, 829, 862
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 2 cut(s) 491, 575
BpuMI CCSGG 3 cut(s) 40, 422, 431
BsaHI GRCGYC 2 cut(s) 136, 476
BsaJI CCNNGG 3 cut(s) 827, 850, 861
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse1I ACTGG 2 cut(s) 514, 664
BseDI CCNNGG 3 cut(s) 827, 850, 861
BseGI GGATG 2 cut(s) 168, 837
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMII CTCAG 2 cut(s) 249, 723
BseNI ACTGG 2 cut(s) 514, 664
BseRI GAGGAG 3 cut(s) 44, 635, 749
BseXI GCAGC 5 cut(s) 26, 29, 364, 538, 640
BseYI CCCAGC 1 cut(s) 353
Bsh1285I CGRYCG 2 cut(s) 435, 679
BshFI GGCC 2 cut(s) 542, 709
BsiEI CGRYCG 2 cut(s) 435, 679
BsiHKAI GWGCWC 2 cut(s) 34, 309
BsiSI CCGG 3 cut(s) 40, 421, 431
BslFI GGGAC 2 cut(s) 496, 622
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 2 cut(s) 338, 719
BsmFI GGGAC 2 cut(s) 496, 622
BsnI GGCC 2 cut(s) 542, 709
Bsp1286I GDGCHC 2 cut(s) 34, 309
BspACI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 754
BspANI GGCC 2 cut(s) 542, 709
BspCNI CTCAG 2 cut(s) 250, 724
BspLI GGNNCC 3 cut(s) 44, 396, 419
BsrI ACTGG 2 cut(s) 514, 664
BssECI CCNNGG 3 cut(s) 827, 850, 861
BssNI GRCGYC 2 cut(s) 136, 476
BssT1I CCWWGG 1 cut(s) 861
Bst4CI ACNGT 4 cut(s) 406, 454, 637, 694
Bst6I CTCTTC 3 cut(s) 188, 289, 407
BstACI GRCGYC 2 cut(s) 136, 476
BstDEI CTNAG 2 cut(s) 258, 732
BstDSI CCRYGG 1 cut(s) 850
BstF5I GGATG 2 cut(s) 168, 837
BstMAI GTCTC 2 cut(s) 338, 719
BstMCI CGRYCG 2 cut(s) 435, 679
BstMWI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 482, 548
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 3 cut(s) 38, 420, 429
BstV1I GCAGC 5 cut(s) 26, 29, 364, 538, 640
BstV2I GAAGAC 3 cut(s) 152, 829, 862
BsuI GTATCC 2 cut(s) 124, 358
BsuRI GGCC 2 cut(s) 542, 709
BtgI CCRYGG 1 cut(s) 850
BtsCI GGATG 2 cut(s) 168, 837
BtsIMutI CAGTG 1 cut(s) 699
Cfr13I GGNCC 1 cut(s) 394
CseI GACGC 4 cut(s) 144, 383, 454, 465
CviAII CATG 2 cut(s) 605, 612
DdeI CTNAG 2 cut(s) 258, 732
DriI GACNNNNNGTC 1 cut(s) 863
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 3 cut(s) 188, 289, 407
Eam1105I GACNNNNNGTC 1 cut(s) 863
EarI CTCTTC 3 cut(s) 188, 289, 407
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 861
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 2 cut(s) 608, 615
FaiI YATR 4 cut(s) 483, 606, 613, 803
FaqI GGGAC 2 cut(s) 496, 622
FatI CATG 2 cut(s) 604, 611
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 3 cut(s) 401, 426, 435
Fnu4HI GCNGC 6 cut(s) 6, 15, 18, 378, 552, 654
FokI GGATG 2 cut(s) 155, 844
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 6 cut(s) 6, 15, 18, 378, 552, 654
FspBI CTAG 3 cut(s) 317, 626, 868
GluI GCNGC 6 cut(s) 6, 15, 18, 378, 552, 654
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 2 cut(s) 542, 709
HapII CCGG 3 cut(s) 40, 421, 431
HgaI GACGC 4 cut(s) 144, 383, 454, 465
Hin1I GRCGYC 2 cut(s) 136, 476
Hin1II CATG 2 cut(s) 608, 615
HincII GTYRAC 2 cut(s) 427, 436
HindII GTYRAC 2 cut(s) 427, 436
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 281, 568, 575, 697
HpaII CCGG 3 cut(s) 40, 421, 431
HphI GGTGA 4 cut(s) 353, 466, 649, 709
Hpy166II GTNNAC 3 cut(s) 402, 427, 436
Hpy188I TCNGA 4 cut(s) 311, 444, 681, 813
Hpy188III TCNNGA 5 cut(s) 317, 531, 572, 617, 789
Hpy8I GTNNAC 3 cut(s) 402, 427, 436
Hpy99I CGWCG 4 cut(s) 138, 377, 448, 481
HpyAV CCTTC 5 cut(s) 101, 206, 332, 449, 460
HpyCH4III ACNGT 4 cut(s) 406, 454, 637, 694
HpyCH4V TGCA 2 cut(s) 220, 656
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 482, 548
HpyF3I CTNAG 2 cut(s) 258, 732
Hsp92I GRCGYC 2 cut(s) 136, 476
Hsp92II CATG 2 cut(s) 608, 615
LmnI GCTCC 4 cut(s) 29, 37, 48, 622
LpnPI CCDG 9 cut(s) 53, 339, 434, 444, 516, 527, 630, 645, 691
Lsp1109I GCAGC 5 cut(s) 26, 29, 364, 538, 640
LweI GCATC 1 cut(s) 741
MaeI CTAG 3 cut(s) 317, 626, 868
MaeIII GTNAC 2 cut(s) 535, 842
MfeI CAATTG 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 34, 309
MluCI AATT 5 cut(s) 359, 388, 470, 644, 806
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 4 cut(s) 7, 59, 467, 659
MseI TTAA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 3 cut(s) 40, 421, 431
MspR9I CCNGG 3 cut(s) 40, 422, 431
MunI CAATTG 1 cut(s) 644
MwoI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 482, 548
NciI CCSGG 3 cut(s) 40, 422, 431
NlaIII CATG 2 cut(s) 608, 615
NlaIV GGNNCC 3 cut(s) 44, 396, 419
NmuCI GTSAC 1 cut(s) 842
NspI RCATGY 1 cut(s) 615
PciI ACATGT 1 cut(s) 611
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PfeI GAWTC 3 cut(s) 568, 575, 697
PflFI GACNNNGTC 1 cut(s) 431
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 6 cut(s) 7, 16, 19, 379, 553, 655
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PscI ACATGT 1 cut(s) 611
Psp124BI GAGCTC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 44, 396, 419
PspPI GGNCC 1 cut(s) 394
PsyI GACNNNGTC 1 cut(s) 431
SacI GAGCTC 1 cut(s) 34
SalI GTCGAC 2 cut(s) 425, 434
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 6 cut(s) 6, 15, 18, 378, 552, 654
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 40, 422, 431
SduI GDGCHC 2 cut(s) 34, 309
SfaNI GCATC 1 cut(s) 741
SinI GGWCC 1 cut(s) 394
SmlI CTYRAG 2 cut(s) 506, 590
SmoI CTYRAG 2 cut(s) 506, 590
Sse9I AATT 5 cut(s) 359, 388, 470, 644, 806
SsiI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 754
SspMI CTAG 3 cut(s) 317, 626, 868
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 3 cut(s) 38, 420, 429
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 4 cut(s) 406, 454, 637, 694
TaqI TCGA 3 cut(s) 246, 426, 435
TaqII GACCGA 2 cut(s) 665, 841
TasI AATT 5 cut(s) 359, 388, 470, 644, 806
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 568, 575, 697
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 5 cut(s) 14, 17, 377, 551, 653
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 4 cut(s) 157, 279, 339, 401
TspRI CASTG 1 cut(s) 699
Tth111I GACNNNGTC 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 2 cut(s) 388, 806
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 1 cut(s) 615
XmiI GTMKAC 3 cut(s) 401, 426, 435
XspI CTAG 3 cut(s) 317, 626, 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.