RLG00000014044

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010962
Physical Location & Seq
Reverse (-)
46124046 .. 46126252
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014044

Sequence Viewer

Length: 864 bp
ATGGCGGCAGTAGCAGTAGCAGCAGCGGTAGTTGGAGCTCCAACCCGGCTCCACCGTCTCCTCCACCACCCACCACCCTCCTTTTTCCTCAAACCCCTTCTCATCTCCACCACCGCCGTATCCAAACAACCCCAAATGCGACGCCCCAAACTTATCTCCACTTGTCTTCCTTTCATCCCCTCACGCCTCTTCTGCCACTCCCTTCCCGACGTCAAAGCCTCTCTGCAAGCAACCCAAGAACTCGATGAGGAAGCTGAGAATGAAGATGAGAACTTGGACTCTTCTTCTTCTTCTTCTGTACTCGGAAGTCTAGACGATGAAAATGGTGGGGAAGGTGAGACTGAAGCTGGGAAATTGGATACCAACGACGCTGCCCGAATGAATTTGGACCCTTCTACCGTTTTGAAGAGGAACCGGGTCGACCCGGTTGACCTTCCCACGCTTACGGTGAGAGAAAAGAAGGAATTGGCGTCGTATGCTAACAGCTTGGGGAAGAAGCTCAAGTCCCAGTTGGTGGGCAAGTCTGGAGTTACGGCCAATGTTGCTGCTTCTTTTGTAGAGAATCTTGAATCTAATGAGCTTCTCAAGGTTAAAATACATGGGACATGTCCAGAGGAGCTAGATGATACGGTGAAGCAATTGGAGGCTGCAACTGGTTCAGTTGTTGTCGGTCGGATTGGTAGAACAGTGATTCTTTACAGGCCAAGTCTCACCAAGCTGAAAGCTGAGGAGAAAAAAGAGCAGATGCGGAAGATTTTTTTAAAGCGAAAGCAGAGATATTCACGACCATCATCTATGGAATTTCAGAAAAAAGGAAGACCAAGGATGTTTGTGCGTGACAGCCGTGGAAGACCCAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

288

Amino Acids

31.64

Weight (kDa)

9.49

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 148 - 234 2.9e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 514
AccI GTMKAC 1 cut(s) 420
AciI CCGC 4 cut(s) 5, 26, 114, 748
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 382, 800
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 3 cut(s) 142, 210, 470
AfaI GTAC 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 2 cut(s) 406, 569
AhdI GACNNNNNGTC 1 cut(s) 857
AluBI AGCT 9 cut(s) 38, 254, 347, 486, 499, 580, 619, 718, 725
AluI AGCT 9 cut(s) 38, 254, 347, 486, 499, 580, 619, 718, 725
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 3 cut(s) 62, 332, 713
AoxI GGCC 2 cut(s) 534, 701
ApeKI GCWGC 5 cut(s) 20, 23, 371, 545, 647
ApoI RAATTY 2 cut(s) 382, 800
AspS9I GGNCC 1 cut(s) 388
AsuC2I CCSGG 3 cut(s) 46, 416, 425
AsuHPI GGTGA 4 cut(s) 347, 460, 643, 703
AvaII GGWCC 1 cut(s) 388
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BanII GRGCYC 1 cut(s) 40
BbsI GAAGAC 3 cut(s) 158, 823, 856
Bbv12I GWGCWC 1 cut(s) 40
BbvCI CCTCAGC 1 cut(s) 726
BbvI GCAGC 5 cut(s) 32, 35, 358, 532, 634
BccI CCATC 1 cut(s) 796
BceAI ACGGC 3 cut(s) 101, 549, 828
BciVI GTATCC 2 cut(s) 130, 352
BcnI CCSGG 3 cut(s) 46, 416, 425
BcoDI GTCTC 3 cut(s) 62, 332, 713
BfaI CTAG 3 cut(s) 311, 620, 862
BfuI GTATCC 2 cut(s) 130, 352
BisI GCNGC 6 cut(s) 6, 21, 24, 372, 546, 648
BlsI GCNGC 6 cut(s) 7, 22, 25, 373, 547, 649
Bme1390I CCNGG 3 cut(s) 46, 416, 425
Bme18I GGWCC 1 cut(s) 388
BmeRI GACNNNNNGTC 1 cut(s) 857
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 3 cut(s) 50, 390, 413
BmrFI CCNGG 3 cut(s) 46, 416, 425
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 1 cut(s) 735
BmuI ACTGGG 1 cut(s) 502
BpiI GAAGAC 3 cut(s) 158, 823, 856
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 726
BpuEI CTTGAG 2 cut(s) 485, 569
BpuMI CCSGG 3 cut(s) 46, 416, 425
BsaHI GRCGYC 3 cut(s) 142, 210, 470
BsaJI CCNNGG 3 cut(s) 821, 844, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse1I ACTGG 2 cut(s) 508, 658
BseDI CCNNGG 3 cut(s) 821, 844, 855
BseGI GGATG 2 cut(s) 174, 831
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMII CTCAG 2 cut(s) 246, 717
BseNI ACTGG 2 cut(s) 508, 658
BseRI GAGGAG 3 cut(s) 50, 629, 743
BseXI GCAGC 5 cut(s) 32, 35, 358, 532, 634
BseYI CCCAGC 1 cut(s) 347
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 2 cut(s) 536, 703
BsiEI CGRYCG 1 cut(s) 673
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 3 cut(s) 46, 415, 425
BslFI GGGAC 2 cut(s) 490, 616
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 3 cut(s) 62, 332, 713
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 490, 616
BsnI GGCC 2 cut(s) 536, 703
Bsp1286I GDGCHC 1 cut(s) 40
BspACI CCGC 4 cut(s) 5, 26, 114, 748
BspANI GGCC 2 cut(s) 536, 703
BspCNI CTCAG 2 cut(s) 247, 718
BspLI GGNNCC 3 cut(s) 50, 390, 413
BsrI ACTGG 2 cut(s) 508, 658
BssECI CCNNGG 3 cut(s) 821, 844, 855
BssNI GRCGYC 3 cut(s) 142, 210, 470
BssT1I CCWWGG 2 cut(s) 821, 855
Bst4CI ACNGT 5 cut(s) 56, 400, 448, 631, 688
Bst6I CTCTTC 3 cut(s) 194, 286, 401
BstACI GRCGYC 3 cut(s) 142, 210, 470
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 255, 726
BstDSI CCRYGG 1 cut(s) 844
BstF5I GGATG 2 cut(s) 174, 831
BstMAI GTCTC 3 cut(s) 62, 332, 713
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 192, 476, 542
BstNSI RCATGY 1 cut(s) 609
BstSCI CCNGG 3 cut(s) 44, 414, 423
BstV1I GCAGC 5 cut(s) 32, 35, 358, 532, 634
BstV2I GAAGAC 3 cut(s) 158, 823, 856
BsuI GTATCC 2 cut(s) 130, 352
BsuRI GGCC 2 cut(s) 536, 703
BtgI CCRYGG 1 cut(s) 844
BtsCI GGATG 2 cut(s) 174, 831
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 388
CseI GACGC 3 cut(s) 150, 377, 459
Csp6I GTAC 1 cut(s) 299
CviAII CATG 2 cut(s) 599, 606
CviQI GTAC 1 cut(s) 299
DdeI CTNAG 2 cut(s) 255, 726
DraI TTTAAA 1 cut(s) 762
DriI GACNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 3 cut(s) 194, 286, 401
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 3 cut(s) 194, 286, 401
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 821, 855
Eco24I GRGCYC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 821, 855
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 2 cut(s) 821, 855
Esp3I CGTCTC 1 cut(s) 62
FaeI CATG 2 cut(s) 602, 609
FaiI YATR 4 cut(s) 477, 600, 607, 797
FaqI GGGAC 2 cut(s) 490, 616
FatI CATG 2 cut(s) 598, 605
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 6 cut(s) 6, 21, 24, 372, 546, 648
FokI GGATG 2 cut(s) 161, 838
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 6 cut(s) 6, 21, 24, 372, 546, 648
FspBI CTAG 3 cut(s) 311, 620, 862
GluI GCNGC 6 cut(s) 6, 21, 24, 372, 546, 648
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 536, 703
HapII CCGG 3 cut(s) 46, 415, 425
HgaI GACGC 3 cut(s) 150, 377, 459
Hin1I GRCGYC 3 cut(s) 142, 210, 470
Hin1II CATG 2 cut(s) 602, 609
HincII GTYRAC 2 cut(s) 421, 430
HindII GTYRAC 2 cut(s) 421, 430
HinfI GANTC 4 cut(s) 278, 562, 569, 691
HpaII CCGG 3 cut(s) 46, 415, 425
HphI GGTGA 4 cut(s) 347, 460, 643, 703
Hpy166II GTNNAC 2 cut(s) 421, 430
Hpy188I TCNGA 3 cut(s) 305, 675, 807
Hpy188III TCNNGA 6 cut(s) 206, 311, 525, 566, 611, 783
Hpy8I GTNNAC 2 cut(s) 421, 430
Hpy99I CGWCG 4 cut(s) 144, 212, 371, 475
HpyAV CCTTC 6 cut(s) 107, 212, 326, 402, 443, 454
HpyCH4III ACNGT 5 cut(s) 56, 400, 448, 631, 688
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 226, 650
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 192, 476, 542
HpyF3I CTNAG 2 cut(s) 255, 726
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 3 cut(s) 142, 210, 470
Hsp92II CATG 2 cut(s) 602, 609
LmnI GCTCC 4 cut(s) 35, 43, 54, 616
LpnPI CCDG 9 cut(s) 59, 333, 428, 438, 510, 521, 624, 639, 685
Lsp1109I GCAGC 5 cut(s) 32, 35, 358, 532, 634
LweI GCATC 1 cut(s) 735
MaeI CTAG 3 cut(s) 311, 620, 862
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 529, 836
MfeI CAATTG 1 cut(s) 638
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 5 cut(s) 353, 382, 464, 638, 800
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 3 cut(s) 13, 65, 653
MseI TTAA 2 cut(s) 591, 761
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 3 cut(s) 46, 415, 425
MspR9I CCNGG 3 cut(s) 46, 416, 425
MunI CAATTG 1 cut(s) 638
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 192, 476, 542
NciI CCSGG 3 cut(s) 46, 416, 425
NlaIII CATG 2 cut(s) 602, 609
NlaIV GGNNCC 3 cut(s) 50, 390, 413
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 1 cut(s) 609
PciI ACATGT 1 cut(s) 605
PfeI GAWTC 3 cut(s) 562, 569, 691
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 6 cut(s) 7, 22, 25, 373, 547, 649
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 3 cut(s) 50, 390, 413
PspPI GGNCC 1 cut(s) 388
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
SacI GAGCTC 1 cut(s) 40
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 591, 761
SatI GCNGC 6 cut(s) 6, 21, 24, 372, 546, 648
Sau96I GGNCC 1 cut(s) 388
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 46, 416, 425
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 1 cut(s) 735
SinI GGWCC 1 cut(s) 388
SmlI CTYRAG 2 cut(s) 500, 584
SmoI CTYRAG 2 cut(s) 500, 584
Sse9I AATT 5 cut(s) 353, 382, 464, 638, 800
SsiI CCGC 4 cut(s) 5, 26, 114, 748
SspMI CTAG 3 cut(s) 311, 620, 862
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 3 cut(s) 44, 414, 423
StyI CCWWGG 2 cut(s) 821, 855
TaaI ACNGT 5 cut(s) 56, 400, 448, 631, 688
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 243, 420
TaqII GACCGA 1 cut(s) 659
TasI AATT 5 cut(s) 353, 382, 464, 638, 800
TatI WGTACW 1 cut(s) 298
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 562, 569, 691
Tru1I TTAA 2 cut(s) 591, 761
Tru9I TTAA 2 cut(s) 591, 761
TscAI CASTG 1 cut(s) 693
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 5 cut(s) 20, 23, 371, 545, 647
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 4 cut(s) 163, 276, 333, 395
TspRI CASTG 1 cut(s) 693
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 2 cut(s) 382, 800
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 3 cut(s) 311, 620, 862
ZraI GACGTC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.