MD10G1065400.v1.1

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010962
Physical Location & Seq
Reverse (-)
8936010 .. 8938323
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1065400.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCGGCGGCAGCAGCAGCAGTAGGAGCTCCAACCTGTCACCGGCTCCACCTCCTCCTCCACCGCCCACCACCACCATCATCTTCTTCCTTCTCCCTTTTCCTCAAACCCCTTCTCACCTCCACCACCACCGTCACCACCACCACCACCACCATCACCATTCCATTCTCGCACCTCCCACGCCGCTCACTTATCTCCCATTCCCTTCCTTCCTTCTCCTCTCGACCTCTCTTCTTCTGCCACTCTCCCACCGCTACCCACTCTCCTCCTCCCCCCAAAGCCTCTTTACAAGAAGAAGAAAAAACCCAACAACTCGAAATTCCAGAAGATAATGATAATGGTGAAGAAATTGAGATCGAAATGGAGAACTCGGATAATAAAGAGGATAGCCAAGTGAGTTCGGAGTCTTCACCTGCTCTAAAGAGAGATGGGGTGAAGCCGCCGACGCTGACGGTGAAAGAAAAGAAGGAACTTGCGTCTTATGCTCACAGCTTGGGGAAGAAGCTCAAGTCCCAGTTGGTGGGGAAGTCCGGCGTCACAGCTAGCGTCGCCGCTTCTTTTATAGAGAACCTTGAATCCAATGAGCTTCTCAAGGTGAAAATACACGGGACATGTCCTGGTGAGCTAGAGGATGCCGTTACACAATTAGAGGAATCAACTGGTTCAGTTGTTGTTGGCCAAATTGGTAGAACAGTGATTCTTTACAGGCCCAGCATCACCAAGTTGAAAGCAGAGGAGAAAAGGCAGCAGTCGCGAAAGATATTCATGAGACGAAAGACATATTCGAGACCCACAGCAATGGAATTTCAGAAAAAGGGAGAAAGACCAAGAATGAATGGGCGTGACAGCCGAGGGAGAACCAGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

290

Amino Acids

31.82

Weight (kDa)

9.47

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 150 - 236 2.3e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 421
Acc36I ACCTGC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 520
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 754
AciI CCGC 7 cut(s) 5, 8, 64, 184, 252, 440, 552
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 2 cut(s) 318, 803
AcyI GRCGYC 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 42, 520
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 2 cut(s) 575, 727
AjnI CCWGG 2 cut(s) 616, 860
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 6 cut(s) 29, 492, 505, 542, 586, 625
AluI AGCT 6 cut(s) 29, 492, 505, 542, 586, 625
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 2 cut(s) 763, 781
AoxI GGCC 2 cut(s) 676, 707
ApeKI GCWGC 4 cut(s) 11, 14, 17, 745
ApoI RAATTY 2 cut(s) 318, 803
AspS9I GGNCC 1 cut(s) 708
AsuNHI GCTAGC 1 cut(s) 542
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 4 cut(s) 23, 26, 29, 757
BccI CCATC 3 cut(s) 85, 161, 422
BceAI ACGGC 1 cut(s) 620
BciT130I CCWGG 2 cut(s) 618, 862
BcoDI GTCTC 2 cut(s) 763, 781
BfaI CTAG 2 cut(s) 543, 626
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 9 cut(s) 6, 9, 12, 15, 18, 184, 440, 552, 746
BlsI GCNGC 9 cut(s) 7, 10, 13, 16, 19, 185, 441, 553, 747
Bme1390I CCNGG 2 cut(s) 618, 862
BmgT120I GGNCC 1 cut(s) 708
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 2 cut(s) 618, 862
BmrI ACTGGG 1 cut(s) 508
BmsI GCATC 2 cut(s) 622, 723
BmtI GCTAGC 1 cut(s) 546
BmuI ACTGGG 1 cut(s) 508
BpiI GAAGAC 1 cut(s) 399
BpuEI CTTGAG 2 cut(s) 491, 575
BsaHI GRCGYC 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 2 cut(s) 850, 861
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 520
Bse118I RCCGGY 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 514, 664
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 2 cut(s) 618, 862
BseDI CCNNGG 2 cut(s) 850, 861
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 2 cut(s) 42, 520
BseMI GCAATG 1 cut(s) 804
BseNI ACTGG 2 cut(s) 514, 664
BseRI GAGGAG 6 cut(s) 44, 47, 208, 255, 258, 749
BseXI GCAGC 4 cut(s) 23, 26, 29, 757
BseYI CCCAGC 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 2 cut(s) 678, 709
BsiHKAI GWGCWC 1 cut(s) 31
BsiSI CCGG 2 cut(s) 43, 531
BslFI GGGAC 2 cut(s) 496, 622
BslI CCNNNNNNNGG 2 cut(s) 42, 520
BsmAI GTCTC 2 cut(s) 763, 781
BsmBI CGTCTC 1 cut(s) 763
BsmFI GGGAC 2 cut(s) 496, 622
BsnI GGCC 2 cut(s) 678, 709
Bso31I GGTCTC 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 354
Bsp68I TCGCGA 1 cut(s) 754
BspACI CCGC 7 cut(s) 5, 8, 64, 184, 252, 440, 552
BspANI GGCC 2 cut(s) 678, 709
BspFNI CGCG 1 cut(s) 754
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 1 cut(s) 47
BspMI ACCTGC 1 cut(s) 421
BspOI GCTAGC 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 781
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 42
BsrI ACTGG 2 cut(s) 514, 664
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 2 cut(s) 850, 861
BssMI GATC 1 cut(s) 354
BssNI GRCGYC 1 cut(s) 534
Bst2UI CCWGG 2 cut(s) 618, 862
Bst4CI ACNGT 3 cut(s) 133, 454, 694
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 544
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 1 cut(s) 754
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 2 cut(s) 763, 781
BstMBI GATC 1 cut(s) 354
BstMWI GCNNNNNNNGC 8 cut(s) 11, 14, 17, 26, 445, 482, 548, 751
BstNI CCWGG 2 cut(s) 618, 862
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 2 cut(s) 616, 860
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 4 cut(s) 23, 26, 29, 757
BstV2I GAAGAC 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 799
BsuRI GGCC 2 cut(s) 678, 709
BtsCI GGATG 1 cut(s) 637
BtsIMutI CAGTG 1 cut(s) 699
BtuMI TCGCGA 1 cut(s) 754
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 544
CciI TCATGA 1 cut(s) 765
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 708
CseI GACGC 4 cut(s) 454, 465, 523, 535
CviAII CATG 2 cut(s) 612, 766
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 781
Eco53kI GAGCTC 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 616, 860
EcoT38I GRGCYC 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 763
FaeI CATG 2 cut(s) 615, 769
FaiI YATR 5 cut(s) 483, 563, 613, 767, 781
FaqI GGGAC 2 cut(s) 496, 622
FatI CATG 2 cut(s) 611, 765
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 15, 18, 184, 440, 552, 746
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 15, 18, 184, 440, 552, 746
FspBI CTAG 2 cut(s) 543, 626
GluI GCNGC 9 cut(s) 6, 9, 12, 15, 18, 184, 440, 552, 746
GsaI CCCAGC 1 cut(s) 714
HaeIII GGCC 2 cut(s) 678, 709
HapII CCGG 2 cut(s) 43, 531
HgaI GACGC 4 cut(s) 454, 465, 523, 535
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 2 cut(s) 615, 769
HinfI GANTC 4 cut(s) 404, 575, 653, 697
HpaII CCGG 2 cut(s) 43, 531
Hpy188I TCNGA 3 cut(s) 373, 403, 810
Hpy188III TCNNGA 5 cut(s) 222, 323, 753, 766, 786
Hpy99I CGWCG 2 cut(s) 448, 551
HpyAV CCTTC 6 cut(s) 100, 122, 215, 219, 223, 460
HpyCH4III ACNGT 3 cut(s) 133, 454, 694
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 14, 17, 26, 445, 482, 548, 751
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 2 cut(s) 615, 769
Kzo9I GATC 1 cut(s) 354
LmnI GCTCC 3 cut(s) 26, 34, 51
Lsp1109I GCAGC 4 cut(s) 23, 26, 29, 757
LweI GCATC 2 cut(s) 622, 723
MaeI CTAG 2 cut(s) 543, 626
MaeIII GTNAC 5 cut(s) 38, 133, 535, 637, 842
MalI GATC 1 cut(s) 356
MbiI CCGCTC 1 cut(s) 186
MboI GATC 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 5 cut(s) 318, 348, 644, 681, 803
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 56
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 797
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 2 cut(s) 43, 531
MspR9I CCNGG 2 cut(s) 618, 862
MvaI CCWGG 2 cut(s) 618, 862
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 8 cut(s) 11, 14, 17, 26, 445, 482, 548, 751
NdeII GATC 1 cut(s) 354
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 2 cut(s) 615, 769
NlaIV GGNNCC 1 cut(s) 47
NmuCI GTSAC 4 cut(s) 38, 133, 535, 842
NruI TCGCGA 1 cut(s) 754
NspI RCATGY 1 cut(s) 615
PagI TCATGA 1 cut(s) 765
PaqCI CACCTGC 1 cut(s) 421
PciI ACATGT 1 cut(s) 611
PfeI GAWTC 3 cut(s) 575, 653, 697
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 9 cut(s) 7, 10, 13, 16, 19, 185, 441, 553, 747
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PscI ACATGT 1 cut(s) 611
Psp124BI GAGCTC 1 cut(s) 31
Psp6I CCWGG 2 cut(s) 616, 860
PspFI CCCAGC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 616, 860
PspN4I GGNNCC 1 cut(s) 47
PspPI GGNCC 1 cut(s) 708
RruI TCGCGA 1 cut(s) 754
RseI CAYNNNNRTG 1 cut(s) 797
SacI GAGCTC 1 cut(s) 31
SatI GCNGC 9 cut(s) 6, 9, 12, 15, 18, 184, 440, 552, 746
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 1 cut(s) 708
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 618, 862
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 2 cut(s) 622, 723
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 2 cut(s) 506, 590
SmoI CTYRAG 2 cut(s) 506, 590
Sse9I AATT 5 cut(s) 318, 348, 644, 681, 803
SsiI CCGC 7 cut(s) 5, 8, 64, 184, 252, 440, 552
SspMI CTAG 2 cut(s) 543, 626
SstI GAGCTC 1 cut(s) 31
StyD4I CCNGG 2 cut(s) 616, 860
TaaI ACNGT 3 cut(s) 133, 454, 694
TaqI TCGA 4 cut(s) 223, 315, 357, 785
TasI AATT 5 cut(s) 318, 348, 644, 681, 803
TauI GCSGC 5 cut(s) 8, 11, 186, 442, 554
TfiI GAWTC 3 cut(s) 575, 653, 697
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 4 cut(s) 38, 133, 535, 842
TseI GCWGC 4 cut(s) 11, 14, 17, 745
Tsp45I GTSAC 4 cut(s) 38, 133, 535, 842
TspDTI ATGAA 2 cut(s) 754, 848
TspRI CASTG 1 cut(s) 699
Van91I CCANNNNNTGG 1 cut(s) 520
XapI RAATTY 2 cut(s) 318, 803
XceI RCATGY 1 cut(s) 615
XspI CTAG 2 cut(s) 543, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.