AT4G39040

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010962
Physical Location & Seq
Reverse (-)
18189012 .. 18190608
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39040.2

Sequence Viewer

Length: 891 bp
ATGGAGAGAGTGACAGCGTCAGCGTCTTGTTCATCGACTCACCTTATCCACAATCTTCTCCGACATCCCAAACCACCGTCTCCGGTCTGTCTCTTCCTCAGACCCTTTTGCTTCTCCGCTTCAGTCTCTCAATCCAATCACCGTAACAAGCCTCGATTTCAATCATTCTCCTCAAAGCCGCTTCCTTGTCATTCTGCTTCACTCGTAGTGAAATCCTTCTCTTCCATCGATGAACCAGATCTCGAAGAAGACGAAGAGAGCGAGGATGAAGATTTCAGCGCAGAAGAATACGAATACGAAGACGAAGAAGAAGAAGATGAGCAGGACAGTGGAGTGGTGGTTTCCGAGAGAGGAATTGAAGATTCGGAAGCATCTGAGGAGGTTTCGGAAATAGGAGATAAAGAGGAGAAAACAGAGAATACGAAGAAGAAGAAGAGTCGAGGCTCTGCTTTGAAACTGTCGATAAAGGAGAAAAAGGAATTGGCTTCTTACGCGCATAGTTTGGGTGATAAATTGAAATGTCAACTCGTCGGCAAATCTGGTGTTACTGATTCTGTCGTTTTCTCATTCCTCGAGACTCTCGAGAAGAACGAGCTCTTAAAGGTGAAAATACGCAAGACATCGCCCGATGAGCTAGAGGATGCGGTGCAGCATTTGGAGGAAGCGACTGGTTCCGTAGCAGTAGGGCAAATAGGGCGGACTGTGATATTGTATAGGCCCAGTCCCACCAAAATGAAAGCAGAGGCGAAGAAGAAGGAAGTTGAGAGAATGTCAATTACTAGAAGGCAGAAATACGCAAACACTAGACCCACTAAACCCTTTAGAAGAGAGTATTCAGAGAGACCTGATGGACGTGGACGCAGAGGCGGAAGTAGAGTTCCAACGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

296

Amino Acids

33.27

Weight (kDa)

5.82

Isoelectric Point (pI)

62.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 153 - 239 5.8e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 494
AciI CCGC 5 cut(s) 117, 179, 644, 697, 867
AcuI CTGAAG 1 cut(s) 105
AgsI TTSAA 4 cut(s) 161, 359, 454, 517
AjiI CACGTC 1 cut(s) 854
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AloI GAACNNNNNNTCC 1 cut(s) 861
AluBI AGCT 2 cut(s) 595, 634
AluI AGCT 2 cut(s) 595, 634
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 5 cut(s) 84, 95, 130, 569, 835
Ama87I CYCGRG 2 cut(s) 572, 581
AoxI GGCC 1 cut(s) 716
ApeKI GCWGC 1 cut(s) 649
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 281, 496
AspS9I GGNCC 1 cut(s) 717
AsuHPI GGTGA 4 cut(s) 32, 131, 518, 616
AvaI CYCGRG 2 cut(s) 572, 581
BanII GRGCYC 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 255, 306
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 661
BccI CCATC 2 cut(s) 233, 842
BcoDI GTCTC 5 cut(s) 84, 95, 130, 569, 835
BfaI CTAG 3 cut(s) 635, 780, 804
BglII AGATCT 1 cut(s) 238
BisI GCNGC 2 cut(s) 179, 650
BlsI GCNGC 2 cut(s) 180, 651
BmeT110I CYCGRG 2 cut(s) 572, 581
BmgBI CACGTC 1 cut(s) 854
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 673
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 2 cut(s) 380, 631
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 2 cut(s) 255, 306
Bsa29I ATCGAT 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 835
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 2 cut(s) 673, 720
Bse8I GATNNNNATC 1 cut(s) 160
BseCI ATCGAT 1 cut(s) 228
BseGI GGATG 3 cut(s) 64, 271, 646
BseJI GATNNNNATC 1 cut(s) 160
BseMII CTCAG 2 cut(s) 112, 366
BseNI ACTGG 2 cut(s) 673, 720
BseRI GAGGAG 3 cut(s) 160, 392, 419
BseXI GCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 1 cut(s) 718
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 2 cut(s) 572, 581
BsiSI CCGG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 708
BsmAI GTCTC 5 cut(s) 84, 95, 130, 569, 835
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 708
BsnI GGCC 1 cut(s) 718
Bso31I GGTCTC 1 cut(s) 835
BsoBI CYCGRG 2 cut(s) 572, 581
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 5 cut(s) 117, 179, 644, 697, 867
BspANI GGCC 1 cut(s) 718
BspCNI CTCAG 2 cut(s) 111, 367
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 1 cut(s) 673
BspTNI GGTCTC 1 cut(s) 835
BsrI ACTGG 2 cut(s) 673, 720
BssMI GATC 1 cut(s) 238
Bst4CI ACNGT 5 cut(s) 78, 143, 329, 459, 703
Bst6I CTCTTC 5 cut(s) 98, 226, 249, 428, 820
BstDEI CTNAG 2 cut(s) 98, 375
BstF5I GGATG 3 cut(s) 64, 271, 646
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 2 cut(s) 281, 496
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 5 cut(s) 84, 95, 130, 569, 835
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 491, 631, 694
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 661
BstV2I GAAGAC 2 cut(s) 255, 306
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 1 cut(s) 718
BsuTUI ATCGAT 1 cut(s) 228
BtgZI GCGATG 1 cut(s) 606
BtrI CACGTC 1 cut(s) 854
BtsCI GGATG 3 cut(s) 64, 271, 646
BtsIMutI CAGTG 1 cut(s) 334
CfoI GCGC 2 cut(s) 281, 496
Cfr13I GGNCC 1 cut(s) 717
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 3 cut(s) 6, 12, 867
CviJI RGCY 8 cut(s) 151, 178, 444, 485, 595, 634, 718, 887
CviKI_1 RGCY 8 cut(s) 151, 178, 444, 485, 595, 634, 718, 887
DdeI CTNAG 2 cut(s) 98, 375
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Eam1104I CTCTTC 5 cut(s) 98, 226, 249, 428, 820
EarI CTCTTC 5 cut(s) 98, 226, 249, 428, 820
EciI GGCGGA 2 cut(s) 712, 882
Ecl136II GAGCTC 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 1 cut(s) 835
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 2 cut(s) 572, 581
EcoICRI GAGCTC 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 597
Esp3I CGTCTC 1 cut(s) 84
FaiI YATR 2 cut(s) 498, 714
FaqI GGGAC 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 179, 650
FokI GGATG 3 cut(s) 51, 278, 653
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 2 cut(s) 179, 650
FspBI CTAG 3 cut(s) 635, 780, 804
GlaI GCGC 2 cut(s) 280, 495
GluI GCNGC 2 cut(s) 179, 650
HaeIII GGCC 1 cut(s) 718
HapII CCGG 1 cut(s) 83
HgaI GACGC 3 cut(s) 6, 12, 867
HhaI GCGC 2 cut(s) 281, 496
Hin6I GCGC 2 cut(s) 279, 494
HinP1I GCGC 2 cut(s) 279, 494
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HinfI GANTC 5 cut(s) 37, 362, 436, 551, 577
HpaII CCGG 1 cut(s) 83
HphI GGTGA 4 cut(s) 32, 131, 518, 616
Hpy166II GTNNAC 2 cut(s) 524, 857
Hpy188I TCNGA 7 cut(s) 62, 101, 346, 367, 376, 388, 838
Hpy188III TCNNGA 4 cut(s) 242, 574, 581, 583
Hpy8I GTNNAC 2 cut(s) 524, 857
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 3 cut(s) 226, 748, 777
HpyCH4III ACNGT 5 cut(s) 78, 143, 329, 459, 703
HpyCH4IV ACGT 1 cut(s) 853
HpyCH4V TGCA 1 cut(s) 649
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 631, 694
HpyF3I CTNAG 2 cut(s) 98, 375
HpySE526I ACGT 1 cut(s) 853
HspAI GCGC 2 cut(s) 279, 494
Kzo9I GATC 1 cut(s) 238
LpnPI CCDG 7 cut(s) 96, 249, 308, 525, 654, 733, 858
Lsp1109I GCAGC 1 cut(s) 661
LweI GCATC 2 cut(s) 380, 631
MaeI CTAG 3 cut(s) 635, 780, 804
MaeII ACGT 1 cut(s) 853
MaeIII GTNAC 3 cut(s) 10, 143, 544
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 597
MluCI AATT 4 cut(s) 354, 479, 512, 774
MlyI GAGTC 3 cut(s) 31, 445, 571
MmeI TCCRAC 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 2 cut(s) 599, 889
MslI CAYNNNNRTG 1 cut(s) 731
MspI CCGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 491, 631, 694
NdeII GATC 1 cut(s) 238
NlaIV GGNNCC 1 cut(s) 673
NmuCI GTSAC 1 cut(s) 10
PaeR7I CTCGAG 2 cut(s) 572, 581
PcsI WCGNNNNNNNCGW 4 cut(s) 249, 258, 579, 588
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 362, 551
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 2 cut(s) 180, 651
PleI GAGTC 3 cut(s) 31, 444, 571
PpsI GAGTC 3 cut(s) 31, 444, 571
Psp124BI GAGCTC 1 cut(s) 597
PspN4I GGNNCC 1 cut(s) 673
PspPI GGNCC 1 cut(s) 717
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 2 cut(s) 599, 889
SatI GCNGC 2 cut(s) 179, 650
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 717
SchI GAGTC 3 cut(s) 31, 445, 571
SduI GDGCHC 1 cut(s) 597
SetI ASST 7 cut(s) 45, 384, 597, 606, 636, 847, 856
SfaNI GCATC 2 cut(s) 380, 631
Sfr274I CTCGAG 2 cut(s) 572, 581
SlaI CTCGAG 2 cut(s) 572, 581
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 2 cut(s) 572, 581
SmoI CTYRAG 2 cut(s) 572, 581
Sse9I AATT 4 cut(s) 354, 479, 512, 774
SsiI CCGC 5 cut(s) 117, 179, 644, 697, 867
SspMI CTAG 3 cut(s) 635, 780, 804
SstI GAGCTC 1 cut(s) 597
TaaI ACNGT 5 cut(s) 78, 143, 329, 459, 703
TaiI ACGT 1 cut(s) 856
TaqI TCGA 8 cut(s) 35, 154, 228, 243, 439, 461, 573, 582
TasI AATT 4 cut(s) 354, 479, 512, 774
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 2 cut(s) 362, 551
Tru1I TTAA 2 cut(s) 599, 889
Tru9I TTAA 2 cut(s) 599, 889
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 649
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 21, 246, 282, 749
TspGWI ACGGA 1 cut(s) 664
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 16
XhoI CTCGAG 2 cut(s) 572, 581
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 3 cut(s) 635, 780, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.