Rw6G013040

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010962
Physical Location & Seq
Forward (+)
24020146 .. 24022355
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013040.1

Sequence Viewer

Length: 879 bp
ATGGCGGCAGTAGCAGTAGCAGCAGCGGTAGTTGGAGCTCCAACCCGGCTCCACCGCCTCCTCCACCACCCACCACCCTCCTTTTTCCTCAAACCCCTTCTCATCTCCACCACCGCCGTATCCAAACAACCCCAAATGCGACGCCCCAAGCTTATCTCCACTTGTCTTCCTTTCATCCCCTCACGCCTCTTCCGCCACTCCCTTCCCGCCATCAAAGCCTCTCTGCAAGTAGAACAACCAACCCAAGAACTCGATGAGGAAGCTGAGAATGAAGATGAGAACTTGGACTCTTCTTCTTCTTCTTCTTCTTCTGTGCTCGGAAGTCTAGACGATGAAAATGGTGGGGAAGGTGAGACTGAAGCTGGGAAATTGGATACCAACGACGCTGCCCGAATGAATTTGGACCCGTCTACCGTTTTGAAGAGGAACCGGGTCGACCCGGTCGACCTTCCCACGCTTACGGTGAGAGAAAAGAAGGAATTGGCGTCGTATGCTAACAGCTTGGGGAAGAAGCTCAAGTCCCAGTTGGTGGGCAAGTCTGGAGTTACGGCCAATGTGGCTGCTTCTTTTGTAGAGAATCTTGAATCTAATGAGCTTCTCAAGGTTAAAATACATGGGACATGTCCAGAGGAGCTAGATGATACGGTGAAGCAATTGGAGGCTGCAACTGGTTCAGTTGTTGTCGGTCGGATTGGTAGAACAGTGATTCTTTACAGGCCAAGTCTCACCAAGCTAAAAGCTGAGGAGAAAAAAGAGCAGATGCGGAAGATTTTTTTGAAGCGGAAGCAGAGATATTCACGACCATCATCTATGGAATTTCAGAAAAAAGGAAGACCGAGGATGTTTGTGCGTGACAGCCGTGGAAGACCCAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

292

Amino Acids

32.22

Weight (kDa)

9.62

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 153 - 239 3e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 529
AccI GTMKAC 3 cut(s) 410, 435, 444
AciI CCGC 8 cut(s) 5, 26, 55, 114, 193, 207, 763, 781
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 2 cut(s) 397, 815
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 2 cut(s) 142, 485
AfiI CCNNNNNNNGG 1 cut(s) 529
AflIII ACRYGT 1 cut(s) 620
AgsI TTSAA 3 cut(s) 421, 584, 778
AhdI GACNNNNNGTC 1 cut(s) 872
Alw21I GWGCWC 2 cut(s) 40, 318
Alw26I GTCTC 2 cut(s) 347, 728
AoxI GGCC 2 cut(s) 549, 716
ApeKI GCWGC 5 cut(s) 20, 23, 386, 560, 662
ApoI RAATTY 2 cut(s) 397, 815
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 3 cut(s) 46, 431, 440
AsuHPI GGTGA 4 cut(s) 362, 475, 658, 718
AvaII GGWCC 1 cut(s) 403
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BanII GRGCYC 1 cut(s) 40
BbsI GAAGAC 3 cut(s) 158, 838, 871
Bbv12I GWGCWC 2 cut(s) 40, 318
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 5 cut(s) 32, 35, 373, 547, 649
BccI CCATC 2 cut(s) 218, 811
BceAI ACGGC 3 cut(s) 101, 564, 843
BciVI GTATCC 2 cut(s) 130, 367
BcnI CCSGG 3 cut(s) 46, 431, 440
BcoDI GTCTC 2 cut(s) 347, 728
BfaI CTAG 3 cut(s) 326, 635, 877
BfuI GTATCC 2 cut(s) 130, 367
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 6 cut(s) 6, 21, 24, 387, 561, 663
BlsI GCNGC 6 cut(s) 7, 22, 25, 388, 562, 664
Bme1390I CCNGG 3 cut(s) 46, 431, 440
Bme18I GGWCC 1 cut(s) 403
BmeRI GACNNNNNGTC 1 cut(s) 872
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 3 cut(s) 50, 405, 428
BmrFI CCNGG 3 cut(s) 46, 431, 440
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 1 cut(s) 750
BmuI ACTGGG 1 cut(s) 517
BpiI GAAGAC 3 cut(s) 158, 838, 871
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 500, 584
BpuMI CCSGG 3 cut(s) 46, 431, 440
BsaHI GRCGYC 2 cut(s) 142, 485
BsaJI CCNNGG 3 cut(s) 836, 859, 870
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 2 cut(s) 523, 673
BseDI CCNNGG 3 cut(s) 836, 859, 870
BseGI GGATG 2 cut(s) 174, 846
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 255, 732
BseNI ACTGG 2 cut(s) 523, 673
BseRI GAGGAG 3 cut(s) 50, 644, 758
BseXI GCAGC 5 cut(s) 32, 35, 373, 547, 649
BseYI CCCAGC 1 cut(s) 362
Bsh1285I CGRYCG 2 cut(s) 444, 688
BshFI GGCC 2 cut(s) 551, 718
BsiEI CGRYCG 2 cut(s) 444, 688
BsiHKAI GWGCWC 2 cut(s) 40, 318
BsiSI CCGG 3 cut(s) 46, 430, 440
BslFI GGGAC 2 cut(s) 505, 631
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 2 cut(s) 347, 728
BsmFI GGGAC 2 cut(s) 505, 631
BsnI GGCC 2 cut(s) 551, 718
Bsp1286I GDGCHC 2 cut(s) 40, 318
BspACI CCGC 8 cut(s) 5, 26, 55, 114, 193, 207, 763, 781
BspANI GGCC 2 cut(s) 551, 718
BspCNI CTCAG 2 cut(s) 256, 733
BspLI GGNNCC 3 cut(s) 50, 405, 428
BsrI ACTGG 2 cut(s) 523, 673
BssECI CCNNGG 3 cut(s) 836, 859, 870
BssNI GRCGYC 2 cut(s) 142, 485
BssT1I CCWWGG 1 cut(s) 870
Bst4CI ACNGT 4 cut(s) 415, 463, 646, 703
Bst6I CTCTTC 3 cut(s) 194, 295, 416
BstACI GRCGYC 2 cut(s) 142, 485
BstDEI CTNAG 2 cut(s) 264, 741
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 2 cut(s) 174, 846
BstMAI GTCTC 2 cut(s) 347, 728
BstMCI CGRYCG 2 cut(s) 444, 688
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 192, 215, 491, 557
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 3 cut(s) 44, 429, 438
BstV1I GCAGC 5 cut(s) 32, 35, 373, 547, 649
BstV2I GAAGAC 3 cut(s) 158, 838, 871
BsuI GTATCC 2 cut(s) 130, 367
BsuRI GGCC 2 cut(s) 551, 718
BtgI CCRYGG 1 cut(s) 859
BtsCI GGATG 2 cut(s) 174, 846
BtsIMutI CAGTG 1 cut(s) 708
Cfr13I GGNCC 1 cut(s) 403
CseI GACGC 3 cut(s) 150, 392, 474
CviAII CATG 2 cut(s) 614, 621
DdeI CTNAG 2 cut(s) 264, 741
DriI GACNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 3 cut(s) 194, 295, 416
Eam1105I GACNNNNNGTC 1 cut(s) 872
EarI CTCTTC 3 cut(s) 194, 295, 416
EciI GGCGGA 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 870
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 2 cut(s) 617, 624
FaiI YATR 4 cut(s) 492, 615, 622, 812
FaqI GGGAC 2 cut(s) 505, 631
FatI CATG 2 cut(s) 613, 620
FauI CCCGC 1 cut(s) 214
FblI GTMKAC 3 cut(s) 410, 435, 444
Fnu4HI GCNGC 6 cut(s) 6, 21, 24, 387, 561, 663
FokI GGATG 2 cut(s) 161, 853
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 6 cut(s) 6, 21, 24, 387, 561, 663
FspBI CTAG 3 cut(s) 326, 635, 877
GluI GCNGC 6 cut(s) 6, 21, 24, 387, 561, 663
GsaI CCCAGC 1 cut(s) 366
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 2 cut(s) 551, 718
HapII CCGG 3 cut(s) 46, 430, 440
HgaI GACGC 3 cut(s) 150, 392, 474
Hin1I GRCGYC 2 cut(s) 142, 485
Hin1II CATG 2 cut(s) 617, 624
HincII GTYRAC 2 cut(s) 436, 445
HindII GTYRAC 2 cut(s) 436, 445
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 287, 577, 584, 706
HpaII CCGG 3 cut(s) 46, 430, 440
HphI GGTGA 4 cut(s) 362, 475, 658, 718
Hpy166II GTNNAC 3 cut(s) 411, 436, 445
Hpy188I TCNGA 3 cut(s) 320, 690, 822
Hpy188III TCNNGA 5 cut(s) 326, 540, 581, 626, 798
Hpy8I GTNNAC 3 cut(s) 411, 436, 445
Hpy99I CGWCG 3 cut(s) 144, 386, 490
HpyAV CCTTC 5 cut(s) 107, 212, 341, 458, 469
HpyCH4III ACNGT 4 cut(s) 415, 463, 646, 703
HpyCH4V TGCA 2 cut(s) 226, 665
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 192, 215, 491, 557
HpyF3I CTNAG 2 cut(s) 264, 741
Hsp92I GRCGYC 2 cut(s) 142, 485
Hsp92II CATG 2 cut(s) 617, 624
LmnI GCTCC 4 cut(s) 35, 43, 54, 631
LpnPI CCDG 9 cut(s) 59, 348, 443, 453, 525, 536, 639, 654, 700
Lsp1109I GCAGC 5 cut(s) 32, 35, 373, 547, 649
LweI GCATC 1 cut(s) 750
MaeI CTAG 3 cut(s) 326, 635, 877
MaeIII GTNAC 2 cut(s) 544, 851
MfeI CAATTG 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 40, 318
MluCI AATT 5 cut(s) 368, 397, 479, 653, 815
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 3 cut(s) 13, 65, 668
MseI TTAA 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 3 cut(s) 46, 430, 440
MspR9I CCNGG 3 cut(s) 46, 431, 440
MunI CAATTG 1 cut(s) 653
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 192, 215, 491, 557
NciI CCSGG 3 cut(s) 46, 431, 440
NlaIII CATG 2 cut(s) 617, 624
NlaIV GGNNCC 3 cut(s) 50, 405, 428
NmuCI GTSAC 1 cut(s) 851
NspI RCATGY 1 cut(s) 624
PciI ACATGT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 3 cut(s) 577, 584, 706
PflFI GACNNNGTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 529
PkrI GCNGC 6 cut(s) 7, 22, 25, 388, 562, 664
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PscI ACATGT 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 3 cut(s) 50, 405, 428
PspPI GGNCC 1 cut(s) 403
PsyI GACNNNGTC 1 cut(s) 440
SacI GAGCTC 1 cut(s) 40
SalI GTCGAC 2 cut(s) 434, 443
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 6 cut(s) 6, 21, 24, 387, 561, 663
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 46, 431, 440
SduI GDGCHC 2 cut(s) 40, 318
SfaNI GCATC 1 cut(s) 750
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 2 cut(s) 515, 599
SmoI CTYRAG 2 cut(s) 515, 599
Sse9I AATT 5 cut(s) 368, 397, 479, 653, 815
SsiI CCGC 8 cut(s) 5, 26, 55, 114, 193, 207, 763, 781
SspMI CTAG 3 cut(s) 326, 635, 877
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 3 cut(s) 44, 429, 438
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 4 cut(s) 415, 463, 646, 703
TaqI TCGA 3 cut(s) 252, 435, 444
TaqII GACCGA 2 cut(s) 674, 850
TasI AATT 5 cut(s) 368, 397, 479, 653, 815
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 577, 584, 706
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 1 cut(s) 708
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 5 cut(s) 20, 23, 386, 560, 662
Tsp45I GTSAC 1 cut(s) 851
TspDTI ATGAA 4 cut(s) 163, 285, 348, 410
TspRI CASTG 1 cut(s) 708
Tth111I GACNNNGTC 1 cut(s) 440
Van91I CCANNNNNTGG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 2 cut(s) 397, 815
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 624
XmiI GTMKAC 3 cut(s) 410, 435, 444
XspI CTAG 3 cut(s) 326, 635, 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.