Rh6DG135400

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010962
Physical Location & Seq
Forward (+)
19370851 .. 19373470
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG135400.1

Sequence Viewer

Length: 867 bp
ATGGCGGCAGTAGCAGCAGCGGTAGTTGGAGCTCCAACCCGGCTCCACCGCCTCCTCCACCACCCACCACCCTCCTTTTTCCTCAAACCCCTTCTCATCTCCACCACCGCCGTATCCAAACAACCCCAAATGCGACGCCCCAAGCTTATCTCCACTTGTCTTCCTTTCATCCCCTCACGCCTCTTCCGCCACTCCCTTCCCGCCATCAAAGCCTCTCTGCAAGTAGAACAACCAACCCAAGAACTCGATGAGGAAGCTGAGAATGAAGATGAGAACTTGGACTCTTCTTCTTCTTCTTCTGTACTCGGAAGTCTAGACGATGAAAATGGTGAGGAAGGTGAGACTGAAGCTGGGAAATTGGATACCAACGACGCTGCCCGAATGAATTTGGACCCTTCTACCGTTTTGAAGAGGAACCGGGTCGACCCGGTCGACCTTCCGACGCTTACGATGAGAGAAAAGAAGGAATTGGCGTCGTATGCCAACAGCTTGGGGAAGAAGCTCAAGTCCCAGTTGGTGGGCAAGTCTGGAGTTACGGCCAATGTTGCTGCTTCTTTTGTAGAGAATCTTGAATCTAATGAGCTTCTCAAGGTTAAAATACATGGGACATGTCCAGAGGAGCTAGATGATACAGTGAAGCAATTGGAGGCTGCAACTGGTTCAGTTGTTGTCGGTCGGATTGGTAGAACAGTGATTCTTTACAGGCCAAGTCTCACCAAGCTGAAAGCTGAGGAGAAAAAAGAGCAGATGCGGAAGATTTTTTTGAAGCGAAAGCAGAGATATTCACGACCATCATCTATGGAATTTCAGAAAAAAGGAAGACCGAGGATGTTTGTGCGTGACAGCCGTGGAAGACCCAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

288

Amino Acids

31.98

Weight (kDa)

9.55

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 149 - 235 2.6e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21350 AT4G39040 AT4G39040
fragaria_vesca FvH4_2g10110
malus_domestica MD05G1057700.v1.1 MD10G1065400.v1.1
prunus_persica Prupe.8G087400_v2.0.a1
pyrus_communis pycom05g04880
rosa_chinensis RchiOBHm_Chr6g0267071
rosa_laevigata RLG00000014044
rosa_multiflora Rmu_co8165610.1_g000001 Rmu_sc0002938.1_g000055 Rmu_ssc0000388.1_g000005
rosa_roxburghii Rroxscaffold_7G00201170
rosa_rugosa Rorug06G0028900
rosa_samantha Rh6AG150500 Rh6BG151300 Rh6CG146700 Rh6DG135400
rosa_wichuraiana Rw6G013040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 517
AccI GTMKAC 2 cut(s) 423, 432
AciI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 751
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 385, 803
AcuI CTGAAG 1 cut(s) 366
AcyI GRCGYC 2 cut(s) 136, 473
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 608
AgsI TTSAA 3 cut(s) 409, 572, 766
AhdI GACNNNNNGTC 1 cut(s) 860
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 2 cut(s) 335, 716
AoxI GGCC 2 cut(s) 537, 704
ApeKI GCWGC 5 cut(s) 14, 17, 374, 548, 650
ApoI RAATTY 2 cut(s) 385, 803
AspS9I GGNCC 1 cut(s) 391
AsuC2I CCSGG 3 cut(s) 40, 419, 428
AsuHPI GGTGA 3 cut(s) 341, 350, 706
AvaII GGWCC 1 cut(s) 391
BaeI ACNNNNGTAYC 2 cut(s) 96, 129
BanII GRGCYC 1 cut(s) 34
BbsI GAAGAC 3 cut(s) 152, 826, 859
Bbv12I GWGCWC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 5 cut(s) 26, 29, 361, 535, 637
BccI CCATC 2 cut(s) 212, 799
BceAI ACGGC 3 cut(s) 95, 552, 831
BciVI GTATCC 2 cut(s) 124, 355
BcnI CCSGG 3 cut(s) 40, 419, 428
BcoDI GTCTC 2 cut(s) 335, 716
BfaI CTAG 3 cut(s) 314, 623, 865
BfuI GTATCC 2 cut(s) 124, 355
BisI GCNGC 6 cut(s) 6, 15, 18, 375, 549, 651
BlsI GCNGC 6 cut(s) 7, 16, 19, 376, 550, 652
Bme1390I CCNGG 3 cut(s) 40, 419, 428
Bme18I GGWCC 1 cut(s) 391
BmeRI GACNNNNNGTC 1 cut(s) 860
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 3 cut(s) 44, 393, 416
BmrFI CCNGG 3 cut(s) 40, 419, 428
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 738
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 3 cut(s) 152, 826, 859
BpmI CTGGAG 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 488, 572
BpuMI CCSGG 3 cut(s) 40, 419, 428
BsaHI GRCGYC 2 cut(s) 136, 473
BsaJI CCNNGG 3 cut(s) 824, 847, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse1I ACTGG 2 cut(s) 511, 661
BseDI CCNNGG 3 cut(s) 824, 847, 858
BseGI GGATG 2 cut(s) 168, 834
BseLI CCNNNNNNNGG 1 cut(s) 517
BseMII CTCAG 2 cut(s) 249, 720
BseNI ACTGG 2 cut(s) 511, 661
BseRI GAGGAG 3 cut(s) 44, 632, 746
BseXI GCAGC 5 cut(s) 26, 29, 361, 535, 637
BseYI CCCAGC 1 cut(s) 350
Bsh1285I CGRYCG 2 cut(s) 432, 676
BshFI GGCC 2 cut(s) 539, 706
BsiEI CGRYCG 2 cut(s) 432, 676
BsiHKAI GWGCWC 1 cut(s) 34
BsiSI CCGG 3 cut(s) 40, 418, 428
BslFI GGGAC 2 cut(s) 493, 619
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 2 cut(s) 335, 716
BsmFI GGGAC 2 cut(s) 493, 619
BsnI GGCC 2 cut(s) 539, 706
Bsp1286I GDGCHC 1 cut(s) 34
BspACI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 751
BspANI GGCC 2 cut(s) 539, 706
BspCNI CTCAG 2 cut(s) 250, 721
BspLI GGNNCC 3 cut(s) 44, 393, 416
BsrI ACTGG 2 cut(s) 511, 661
BssECI CCNNGG 3 cut(s) 824, 847, 858
BssNI GRCGYC 2 cut(s) 136, 473
BssT1I CCWWGG 1 cut(s) 858
Bst4CI ACNGT 3 cut(s) 403, 634, 691
Bst6I CTCTTC 3 cut(s) 188, 289, 404
BstACI GRCGYC 2 cut(s) 136, 473
BstDEI CTNAG 2 cut(s) 258, 729
BstDSI CCRYGG 1 cut(s) 847
BstF5I GGATG 2 cut(s) 168, 834
BstMAI GTCTC 2 cut(s) 335, 716
BstMCI CGRYCG 2 cut(s) 432, 676
BstMWI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 479, 545
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 3 cut(s) 38, 417, 426
BstV1I GCAGC 5 cut(s) 26, 29, 361, 535, 637
BstV2I GAAGAC 3 cut(s) 152, 826, 859
BstXI CCANNNNNNTGG 1 cut(s) 490
BsuI GTATCC 2 cut(s) 124, 355
BsuRI GGCC 2 cut(s) 539, 706
BtgI CCRYGG 1 cut(s) 847
BtsCI GGATG 2 cut(s) 168, 834
BtsIMutI CAGTG 2 cut(s) 639, 696
Cfr13I GGNCC 1 cut(s) 391
CseI GACGC 4 cut(s) 144, 380, 451, 462
Csp6I GTAC 1 cut(s) 302
CviAII CATG 2 cut(s) 602, 609
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 2 cut(s) 258, 729
DriI GACNNNNNGTC 1 cut(s) 860
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 3 cut(s) 188, 289, 404
Eam1105I GACNNNNNGTC 1 cut(s) 860
EarI CTCTTC 3 cut(s) 188, 289, 404
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 858
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 858
FaeI CATG 2 cut(s) 605, 612
FaiI YATR 4 cut(s) 480, 603, 610, 800
FaqI GGGAC 2 cut(s) 493, 619
FatI CATG 2 cut(s) 601, 608
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 2 cut(s) 423, 432
Fnu4HI GCNGC 6 cut(s) 6, 15, 18, 375, 549, 651
FokI GGATG 2 cut(s) 155, 841
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 6 cut(s) 6, 15, 18, 375, 549, 651
FspBI CTAG 3 cut(s) 314, 623, 865
GluI GCNGC 6 cut(s) 6, 15, 18, 375, 549, 651
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 2 cut(s) 539, 706
HapII CCGG 3 cut(s) 40, 418, 428
HgaI GACGC 4 cut(s) 144, 380, 451, 462
Hin1I GRCGYC 2 cut(s) 136, 473
Hin1II CATG 2 cut(s) 605, 612
HincII GTYRAC 2 cut(s) 424, 433
HindII GTYRAC 2 cut(s) 424, 433
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 281, 565, 572, 694
HpaII CCGG 3 cut(s) 40, 418, 428
HphI GGTGA 3 cut(s) 341, 350, 706
Hpy166II GTNNAC 2 cut(s) 424, 433
Hpy188I TCNGA 4 cut(s) 308, 441, 678, 810
Hpy188III TCNNGA 5 cut(s) 314, 528, 569, 614, 786
Hpy8I GTNNAC 2 cut(s) 424, 433
Hpy99I CGWCG 4 cut(s) 138, 374, 445, 478
HpyAV CCTTC 6 cut(s) 101, 206, 329, 405, 446, 457
HpyCH4III ACNGT 3 cut(s) 403, 634, 691
HpyCH4V TGCA 2 cut(s) 220, 653
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 479, 545
HpyF3I CTNAG 2 cut(s) 258, 729
Hsp92I GRCGYC 2 cut(s) 136, 473
Hsp92II CATG 2 cut(s) 605, 612
LmnI GCTCC 4 cut(s) 29, 37, 48, 619
LpnPI CCDG 9 cut(s) 53, 336, 431, 441, 513, 524, 627, 642, 688
Lsp1109I GCAGC 5 cut(s) 26, 29, 361, 535, 637
LweI GCATC 1 cut(s) 738
MaeI CTAG 3 cut(s) 314, 623, 865
MaeIII GTNAC 2 cut(s) 532, 839
MfeI CAATTG 1 cut(s) 641
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 5 cut(s) 356, 385, 467, 641, 803
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 4 cut(s) 7, 59, 464, 656
MseI TTAA 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 3 cut(s) 40, 418, 428
MspR9I CCNGG 3 cut(s) 40, 419, 428
MunI CAATTG 1 cut(s) 641
MwoI GCNNNNNNNGC 6 cut(s) 11, 14, 186, 209, 479, 545
NciI CCSGG 3 cut(s) 40, 419, 428
NlaIII CATG 2 cut(s) 605, 612
NlaIV GGNNCC 3 cut(s) 44, 393, 416
NmuCI GTSAC 1 cut(s) 839
NspI RCATGY 1 cut(s) 612
PciI ACATGT 1 cut(s) 608
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PfeI GAWTC 3 cut(s) 565, 572, 694
PflFI GACNNNGTC 1 cut(s) 428
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 6 cut(s) 7, 16, 19, 376, 550, 652
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 44, 393, 416
PspPI GGNCC 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 428
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
SacI GAGCTC 1 cut(s) 34
SalI GTCGAC 2 cut(s) 422, 431
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 6 cut(s) 6, 15, 18, 375, 549, 651
Sau96I GGNCC 1 cut(s) 391
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 40, 419, 428
SduI GDGCHC 1 cut(s) 34
SfaNI GCATC 1 cut(s) 738
SinI GGWCC 1 cut(s) 391
SmlI CTYRAG 2 cut(s) 503, 587
SmoI CTYRAG 2 cut(s) 503, 587
Sse9I AATT 5 cut(s) 356, 385, 467, 641, 803
SsiI CCGC 7 cut(s) 5, 20, 49, 108, 187, 201, 751
SspMI CTAG 3 cut(s) 314, 623, 865
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 3 cut(s) 38, 417, 426
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 3 cut(s) 403, 634, 691
TaqI TCGA 3 cut(s) 246, 423, 432
TaqII GACCGA 2 cut(s) 662, 838
TasI AATT 5 cut(s) 356, 385, 467, 641, 803
TatI WGTACW 1 cut(s) 301
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 565, 572, 694
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 2 cut(s) 639, 696
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 5 cut(s) 14, 17, 374, 548, 650
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 4 cut(s) 157, 279, 336, 398
TspRI CASTG 2 cut(s) 639, 696
Tth111I GACNNNGTC 1 cut(s) 428
Van91I CCANNNNNTGG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 2 cut(s) 385, 803
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 612
XmiI GTMKAC 2 cut(s) 423, 432
XspI CTAG 3 cut(s) 314, 623, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.