AT2G22650

FAD-dependent oxidoreductase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007219
Physical Location & Seq
Reverse (-)
9624505 .. 9627072
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22650.3

Sequence Viewer

Length: 1263 bp
ATGATCACTGCTGTTCTTCACCCTTGTTCTTCGGCGTCTCTCTCTCTTCCCAACCGTCCTCCTCTTAGGTACGCCATCCTTGGTGCAGGTTTTGCTGGAATCTCTGTTGCCTGGCACCTGCTTAAGGAGAGTCCAAAAGAGCTGAGATTAAGTGTTGACGTATATGATGAAGTTGGCATAGGAGGTGGTGCTTCTGGTGTTTCTGGAGGACTCCTCCATCCTTATTCTCCTAAAGGAAAGCTACTTTGGCATGGTGCTGAGTGTTGGAGAGAATGTTTAGAGCTCTTAAATGTGGCTGAAACAGCTGCTTCTTCTAGTTACCAATCTACGGAGAACAGAGACTTTAGCGAGAGTTTCGGAAATTTTATGGTTCGAAGAAGGGGAATCTTGAGACCAGCAACAAATGCCAAGACTTTAGATTTAATGAGTGATAATGCTCGAAACTGTCTTGCTAGTTGTGTAGTAGAGACTATTGACAAAGATGGTGCACAAAATCTCGTCCCCAATCTATACCTGCCTTTGAACTGCGCCTTCTATTTTCCAGGAGCTATGAATGTTAATCCGCACCGATATCTCCAGGCACTCTTTCTAGCATGCCAGAGCTCAGCAAAAACATCACTGGGAAGGAAAAACATAACTTTGGTGAACAAGTCTATAACTGATGTGCTTGAACTTGAAGGAGGATATGATGCAGTAGTAATATGTCTTGGATCCAAGGTAGACTTTCTTCCAGGGCTTACTGGAAAGCTCCCTTTAAGGACATGCCGAGGTGTCATTACTCATATGCAGTTACACGAGAGTGCCAGAGGAAGTTACCCGGACGATGGACCTTCGATCTTGTCAGATGCGTGGCTCGCGGTTCAGGGACCCCGTAATCTGCACATGGGATCCACATGGGAATGGCAATCAAGAAATTATTCATCGGATGTCCCAGCAGAGGAAGCTTCAAGAGCTCTCAACGAGCTTCTTCCGAAAGCAAGTGCAGTTTATCCAGACATAGAAAAGTGGGAATTTGCGGGAGCAAGGGCGGGCTTGAGAGCAATGCCACCTGTCACAAGCCATGGATCACTGCCGCTCTTGGGATGTGTTAATCAGCTCATAGGTGCTGGTCAGTTTTGCAACTTCTGGGTATTTTCAGGGCTCGGATCAAGGGGACTACTCTATCATGCTTGGCTTGGGAAACTCATGTCTAAAGCTGTTTTAAGTTGCAATGAAGAATTACTTCCTTCTGAACTAACTTCTTGGAAGAAAAACAGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

420

Amino Acids

45.68

Weight (kDa)

7.94

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 275 2e-26 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 126
Acc36I ACCTGC 3 cut(s) 77, 126, 522
AccB1I GGYRCC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 1075
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 857
AciI CCGC 5 cut(s) 563, 857, 1016, 1028, 1073
AclWI GGATC 6 cut(s) 705, 718, 882, 895, 1072, 1153
AcsI RAATTY 2 cut(s) 361, 1010
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 5 cut(s) 124, 328, 824, 937, 1079
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 4 cut(s) 523, 671, 677, 948
AjnI CCWGG 4 cut(s) 110, 541, 576, 730
AjuI GAANNNNNNNTTGG 2 cut(s) 229, 261
AleI CACNNNNGTG 1 cut(s) 798
Alw21I GWGCWC 4 cut(s) 285, 490, 605, 955
Alw26I GTCTC 4 cut(s) 42, 333, 385, 461
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 6 cut(s) 705, 718, 882, 895, 1072, 1153
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 2 cut(s) 361, 1010
Asp700I GAANNNNTTC 2 cut(s) 916, 1221
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 2 cut(s) 827, 866
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 2 cut(s) 11, 655
AsuII TTCGAA 1 cut(s) 373
AvaII GGWCC 2 cut(s) 827, 866
BaeGI GKGCMC 1 cut(s) 490
BamHI GGATCC 2 cut(s) 710, 887
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 4 cut(s) 285, 605, 955, 1143
BarI GAAGNNNNNNTAC 2 cut(s) 711, 743
BauI CACGAG 1 cut(s) 794
Bbv12I GWGCWC 4 cut(s) 285, 490, 605, 955
BbvI GCAGC 1 cut(s) 292
BccI CCATC 4 cut(s) 83, 225, 476, 818
BciT130I CCWGG 4 cut(s) 112, 543, 578, 732
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 4 cut(s) 42, 333, 385, 461
BfaI CTAG 3 cut(s) 315, 453, 590
BfrI CTTAAG 1 cut(s) 122
BfuAI ACCTGC 3 cut(s) 77, 126, 522
BisI GCNGC 2 cut(s) 306, 1073
BlpI GCTNAGC 1 cut(s) 604
BlsI GCNGC 2 cut(s) 307, 1074
Bme1390I CCNGG 5 cut(s) 112, 543, 578, 732, 818
Bme18I GGWCC 2 cut(s) 827, 866
BmgT120I GGNCC 2 cut(s) 827, 866
BmiI GGNNCC 5 cut(s) 116, 712, 867, 868, 889
BmrFI CCNGG 5 cut(s) 112, 543, 578, 732, 818
BmrI ACTGGG 1 cut(s) 629
BmsI GCATC 2 cut(s) 679, 835
BmuI ACTGGG 1 cut(s) 629
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 2 cut(s) 225, 560
Bpu1102I GCTNAGC 1 cut(s) 604
Bpu14I TTCGAA 1 cut(s) 373
BpuEI CTTGAG 2 cut(s) 409, 1054
BpuMI CCSGG 1 cut(s) 818
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 385
BsaJI CCNNGG 5 cut(s) 79, 714, 731, 766, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 328, 824, 937, 1079
Bse1I ACTGG 2 cut(s) 624, 745
Bse3DI GCAATG 2 cut(s) 1047, 1216
BseBI CCWGG 4 cut(s) 112, 543, 578, 732
BseDI CCNNGG 5 cut(s) 79, 714, 731, 766, 1060
BseGI GGATG 4 cut(s) 75, 217, 931, 1088
BseLI CCNNNNNNNGG 5 cut(s) 124, 328, 824, 937, 1079
BseMI GCAATG 2 cut(s) 1047, 1216
BseMII CTCAG 3 cut(s) 134, 249, 618
BseNI ACTGG 2 cut(s) 624, 745
BseRI GAGGAG 2 cut(s) 51, 203
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 931
BsgI GTGCAG 3 cut(s) 105, 863, 1002
Bsh1236I CGCG 1 cut(s) 857
BshNI GGYRCC 1 cut(s) 114
BsiHKAI GWGCWC 4 cut(s) 285, 490, 605, 955
BsiSI CCGG 1 cut(s) 818
BslFI GGGAC 4 cut(s) 485, 879, 914, 1167
BslI CCNNNNNNNGG 5 cut(s) 124, 328, 824, 937, 1079
BsmAI GTCTC 4 cut(s) 42, 333, 385, 461
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 485, 879, 914, 1167
Bso31I GGTCTC 1 cut(s) 385
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 5 cut(s) 285, 490, 605, 955, 1143
Bsp143I GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
Bsp1720I GCTNAGC 1 cut(s) 604
Bsp19I CCATGG 1 cut(s) 1060
BspACI CCGC 5 cut(s) 563, 857, 1016, 1028, 1073
BspCNI CTCAG 3 cut(s) 135, 250, 617
BspFNI CGCG 1 cut(s) 857
BspLI GGNNCC 5 cut(s) 116, 712, 867, 868, 889
BspMI ACCTGC 3 cut(s) 77, 126, 522
BspPI GGATC 6 cut(s) 705, 718, 882, 895, 1072, 1153
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 114
BspTI CTTAAG 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 385
BsrBI CCGCTC 1 cut(s) 1075
BsrDI GCAATG 2 cut(s) 1047, 1216
BsrI ACTGG 2 cut(s) 624, 745
BssECI CCNNGG 5 cut(s) 79, 714, 731, 766, 1060
BssMI GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
BssNI GRCGYC 1 cut(s) 35
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 3 cut(s) 79, 714, 1060
Bst2BI CACGAG 1 cut(s) 794
Bst2UI CCWGG 4 cut(s) 112, 543, 578, 732
Bst4CI ACNGT 2 cut(s) 56, 446
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 122
BstAPI GCANNNNNTGC 2 cut(s) 92, 404
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 3 cut(s) 595, 855, 1030
BstDEI CTNAG 4 cut(s) 65, 143, 258, 604
BstDSI CCRYGG 1 cut(s) 1060
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 4 cut(s) 75, 217, 931, 1088
BstFNI CGCG 1 cut(s) 857
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 6 cut(s) 6, 713, 837, 890, 1067, 1148
BstMAI GTCTC 4 cut(s) 42, 333, 385, 461
BstMBI GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
BstMWI GCNNNNNNNGC 7 cut(s) 92, 247, 302, 404, 854, 941, 950
BstNI CCWGG 4 cut(s) 112, 543, 578, 732
BstNSI RCATGY 2 cut(s) 597, 765
BstSCI CCNGG 5 cut(s) 110, 541, 576, 730, 816
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 1 cut(s) 857
BstV1I GCAGC 1 cut(s) 292
BstX2I RGATCY 2 cut(s) 710, 887
BstYI RGATCY 2 cut(s) 710, 887
BtgI CCRYGG 1 cut(s) 1060
BtsCI GGATG 4 cut(s) 75, 217, 931, 1088
BtsI GCAGTG 2 cut(s) 6, 1067
BtsIMutI CAGTG 3 cut(s) 6, 617, 1067
BveI ACCTGC 3 cut(s) 77, 126, 522
Cac8I GCNNGC 3 cut(s) 595, 855, 1030
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 827, 866
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 70
CviAII CATG 8 cut(s) 251, 594, 762, 883, 894, 1061, 1166, 1186
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 4 cut(s) 65, 143, 258, 604
DpnI GATC 6 cut(s) 5, 712, 836, 889, 1066, 1147
DpnII GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 3 cut(s) 283, 603, 953
Eco130I CCWWGG 3 cut(s) 79, 714, 1060
Eco24I GRGCYC 4 cut(s) 285, 605, 955, 1143
Eco31I GGTCTC 1 cut(s) 385
Eco32I GATATC 1 cut(s) 572
Eco47I GGWCC 2 cut(s) 827, 866
Eco53kI GAGCTC 3 cut(s) 283, 603, 953
EcoICRI GAGCTC 3 cut(s) 283, 603, 953
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 866
EcoRII CCWGG 4 cut(s) 110, 541, 576, 730
EcoRV GATATC 1 cut(s) 572
EcoT14I CCWWGG 3 cut(s) 79, 714, 1060
EcoT38I GRGCYC 4 cut(s) 285, 605, 955, 1143
ErhI CCWWGG 3 cut(s) 79, 714, 1060
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 8 cut(s) 254, 597, 765, 886, 897, 1064, 1169, 1189
FalI AAGNNNNNCTT 4 cut(s) 707, 739, 1206, 1238
FaqI GGGAC 4 cut(s) 485, 879, 914, 1167
FatI CATG 8 cut(s) 250, 593, 761, 882, 893, 1060, 1165, 1185
FauI CCCGC 2 cut(s) 1009, 1021
FauNDI CATATG 1 cut(s) 783
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 2 cut(s) 306, 1073
FokI GGATG 4 cut(s) 62, 204, 938, 1095
FriOI GRGCYC 4 cut(s) 285, 605, 955, 1143
Fsp4HI GCNGC 2 cut(s) 306, 1073
FspBI CTAG 3 cut(s) 315, 453, 590
GlaI GCGC 1 cut(s) 529
GluI GCNGC 2 cut(s) 306, 1073
GsaI CCCAGC 1 cut(s) 935
GsuI CTGGAG 2 cut(s) 225, 560
HapII CCGG 1 cut(s) 818
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 530
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 8 cut(s) 254, 597, 765, 886, 897, 1064, 1169, 1189
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 4 cut(s) 99, 130, 210, 384
HpaII CCGG 1 cut(s) 818
HphI GGTGA 2 cut(s) 11, 655
Hpy166II GTNNAC 4 cut(s) 157, 488, 646, 721
Hpy188I TCNGA 6 cut(s) 359, 844, 925, 972, 1145, 1231
Hpy188III TCNNGA 5 cut(s) 204, 388, 909, 948, 992
Hpy8I GTNNAC 4 cut(s) 157, 488, 646, 721
HpyAV CCTTC 6 cut(s) 372, 541, 618, 671, 840, 1236
HpyCH4III ACNGT 2 cut(s) 56, 446
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 8 cut(s) 86, 488, 692, 787, 880, 983, 1119, 1209
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 247, 302, 404, 854, 941, 950
HpyF3I CTNAG 4 cut(s) 65, 143, 258, 604
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 8 cut(s) 254, 597, 765, 886, 897, 1064, 1169, 1189
HspAI GCGC 1 cut(s) 528
KflI GGGWCCC 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
LmnI GCTCC 3 cut(s) 545, 753, 1019
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 2 cut(s) 679, 835
MaeI CTAG 3 cut(s) 315, 453, 590
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 4 cut(s) 317, 789, 812, 1051
MalI GATC 6 cut(s) 5, 712, 836, 889, 1066, 1147
MbiI CCGCTC 1 cut(s) 1075
MboI GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
MboII GAAGA 9 cut(s) 8, 21, 38, 303, 387, 719, 959, 1226, 1258
MflI RGATCY 2 cut(s) 710, 887
MhlI GDGCHC 5 cut(s) 285, 490, 605, 955, 1143
MluCI AATT 4 cut(s) 361, 913, 1010, 1217
MlyI GAGTC 2 cut(s) 139, 204
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 9 cut(s) 69, 72, 176, 200, 224, 674, 761, 800, 931
MroXI GAANNNNTTC 2 cut(s) 916, 1221
MseI TTAA 8 cut(s) 123, 149, 287, 422, 558, 755, 1089, 1202
MslI CAYNNNNRTG 2 cut(s) 798, 898
MspA1I CMGCKG 1 cut(s) 305
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 5 cut(s) 112, 543, 578, 732, 818
MvaI CCWGG 4 cut(s) 112, 543, 578, 732
MvnI CGCG 1 cut(s) 857
MwoI GCNNNNNNNGC 7 cut(s) 92, 247, 302, 404, 854, 941, 950
NciI CCSGG 1 cut(s) 818
NcoI CCATGG 1 cut(s) 1060
NdeI CATATG 1 cut(s) 783
NdeII GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
NlaIII CATG 8 cut(s) 254, 597, 765, 886, 897, 1064, 1169, 1189
NlaIV GGNNCC 5 cut(s) 116, 712, 867, 868, 889
NmeAIII GCCGAG 1 cut(s) 791
NmuCI GTSAC 1 cut(s) 1051
NspI RCATGY 2 cut(s) 597, 765
NspV TTCGAA 1 cut(s) 373
OliI CACNNNNGTG 1 cut(s) 798
PaeI GCATGC 1 cut(s) 597
PaqCI CACCTGC 1 cut(s) 126
PdmI GAANNNNTTC 2 cut(s) 916, 1221
PfeI GAWTC 2 cut(s) 99, 384
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 2 cut(s) 307, 1074
PleI GAGTC 2 cut(s) 138, 204
PpsI GAGTC 2 cut(s) 138, 204
PpuMI RGGWCCY 1 cut(s) 866
Psp124BI GAGCTC 3 cut(s) 285, 605, 955
Psp5II RGGWCCY 1 cut(s) 866
Psp6I CCWGG 4 cut(s) 110, 541, 576, 730
PspFI CCCAGC 1 cut(s) 931
PspGI CCWGG 4 cut(s) 110, 541, 576, 730
PspN4I GGNNCC 5 cut(s) 116, 712, 867, 868, 889
PspPI GGNCC 2 cut(s) 827, 866
PspPPI RGGWCCY 1 cut(s) 866
PsuI RGATCY 2 cut(s) 710, 887
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 2 cut(s) 798, 898
SacI GAGCTC 3 cut(s) 285, 605, 955
SaqAI TTAA 8 cut(s) 123, 149, 287, 422, 558, 755, 1089, 1202
SatI GCNGC 2 cut(s) 306, 1073
Sau3AI GATC 6 cut(s) 3, 710, 834, 887, 1064, 1145
Sau96I GGNCC 2 cut(s) 827, 866
SchI GAGTC 2 cut(s) 139, 204
ScrFI CCNGG 5 cut(s) 112, 543, 578, 732, 818
SduI GDGCHC 5 cut(s) 285, 490, 605, 955, 1143
SfaNI GCATC 2 cut(s) 679, 835
SfuI TTCGAA 1 cut(s) 373
SinI GGWCC 2 cut(s) 827, 866
SmiMI CAYNNNNRTG 2 cut(s) 798, 898
SmlI CTYRAG 3 cut(s) 122, 388, 1033
SmoI CTYRAG 3 cut(s) 122, 388, 1033
SphI GCATGC 1 cut(s) 597
Sse9I AATT 4 cut(s) 361, 913, 1010, 1217
SsiI CCGC 5 cut(s) 563, 857, 1016, 1028, 1073
SspMI CTAG 3 cut(s) 315, 453, 590
SstI GAGCTC 3 cut(s) 285, 605, 955
StyD4I CCNGG 5 cut(s) 110, 541, 576, 730, 816
StyI CCWWGG 3 cut(s) 79, 714, 1060
TaaI ACNGT 2 cut(s) 56, 446
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 373, 439, 833
TasI AATT 4 cut(s) 361, 913, 1010, 1217
TauI GCSGC 1 cut(s) 1075
TfiI GAWTC 2 cut(s) 99, 384
Tru1I TTAA 8 cut(s) 123, 149, 287, 422, 558, 755, 1089, 1202
Tru9I TTAA 8 cut(s) 123, 149, 287, 422, 558, 755, 1089, 1202
TscAI CASTG 3 cut(s) 13, 624, 1074
TseFI GTSAC 1 cut(s) 1051
TseI GCWGC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 1051
TspDTI ATGAA 4 cut(s) 183, 566, 909, 1227
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 3 cut(s) 13, 624, 1074
Vha464I CTTAAG 1 cut(s) 122
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 827, 866
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 2 cut(s) 361, 1010
XceI RCATGY 2 cut(s) 597, 765
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 916, 1221
XspI CTAG 3 cut(s) 315, 453, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.