FvH4_5g35120

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007219
Physical Location & Seq
Reverse (-)
25680274 .. 25682969
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35120.t2

Sequence Viewer

Length: 870 bp
ATGGAGAACATGATGGTGTTGAAAGATAATGCGCAGAATGGCCTGGCCGGTTGCAAAATTGAAACCATTGACGAGGATGCTGCCCAAAATCTTGTACCGGGCATCTGTTCACCTTTTAATACTTCTTTTTATATGCCTCAAGCTGTTAATGTCCATCCTCAGCGCTACCTCGAGGCACTCTTCTTAGCATGTCAAACTTTGGTGAAAGTACTTTGTGCTTCTGGCATGGGGGTAAAGGAACTGCATTTGCGCAAGGACTCCGTTCAAAACCTGCTAGAGTTGGACGGGGAATACCAGGCAGTAATTGTGTGTCTAGGAGCCAAAGCAGATCTGCTTCCTGAGCTCTCTGGGAGGCTTCCATTGAGGAAATGCAGAGGTGTTGTTGCTCATCTGCAGCTTCCTGATAATATGGGAGGGGAGTATCCAGGCTTTGGCCCCTCAATATTGTCAGATGCTTGGCTTGCTGTCCAAGGACCTCGTAGTTTGTATATGGGCTCAACATGGGAATGGAAATCAAGAAATTCATCACCAAATGTATCTGCAGATGAAGCTGCAGAAGCCCCTAAAGAGCTTCTACCAAAAGCATGCGGCATTTATCCGGTTGTTAAAGATTGGACCTTTATTGAAGCCAGGGCTGGTGTTAGGGCAATGCCACCACTCACTCCTCATGGATCACTTCCTCTCTTGGGTTGTGTCAATAACATCATAGGTCAAAATCAAACTTGCATGTACTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTACCATGGTTGGCTTGGGAAGTTGATGGCACAAGCTGTGCTTTCATGTAACGAAGAATTAATTCCATATGAACTAACCTCATGGAAAAGAACAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

290

Amino Acids

31.68

Weight (kDa)

6.22

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 14 - 264 1.1e-23 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 170
Acc16I TGCGCA 2 cut(s) 33, 251
Acc36I ACCTGC 1 cut(s) 279
AccB7I CCANNNNNTGG 1 cut(s) 431
AciI CCGC 1 cut(s) 588
AclWI GGATC 1 cut(s) 679
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 4 cut(s) 96, 210, 731, 770
AfeI AGCGCT 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 431, 686
AgsI TTSAA 5 cut(s) 22, 62, 266, 626, 756
AjnI CCWGG 4 cut(s) 42, 294, 424, 629
AluBI AGCT 6 cut(s) 143, 343, 397, 551, 571, 803
AluI AGCT 6 cut(s) 143, 343, 397, 551, 571, 803
Alw21I GWGCWC 1 cut(s) 345
AlwI GGATC 1 cut(s) 679
Ama87I CYCGRG 1 cut(s) 170
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 3 cut(s) 40, 45, 433
ApeKI GCWGC 3 cut(s) 80, 394, 551
ApoI RAATTY 1 cut(s) 520
AseI ATTAAT 1 cut(s) 827
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 3 cut(s) 34, 165, 252
AspS9I GGNCC 3 cut(s) 434, 473, 615
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 3 cut(s) 102, 214, 519
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 473, 615
BanII GRGCYC 2 cut(s) 345, 497
Bbv12I GWGCWC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 67, 406, 538
BccI CCATC 3 cut(s) 7, 162, 787
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 4 cut(s) 44, 296, 426, 631
BciVI GTATCC 1 cut(s) 432
BcnI CCSGG 1 cut(s) 99
BfaI CTAG 2 cut(s) 275, 314
BfmI CTRYAG 3 cut(s) 392, 540, 552
BfoI RGCGCY 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 279
BfuI GTATCC 1 cut(s) 432
BglII AGATCT 1 cut(s) 328
BisI GCNGC 4 cut(s) 81, 395, 552, 589
BlsI GCNGC 4 cut(s) 82, 396, 553, 590
BmcAI AGTACT 1 cut(s) 210
Bme1390I CCNGG 5 cut(s) 44, 99, 296, 426, 631
Bme18I GGWCC 2 cut(s) 473, 615
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 3 cut(s) 434, 473, 615
BmiI GGNNCC 2 cut(s) 319, 436
BmrFI CCNGG 5 cut(s) 44, 99, 296, 426, 631
BmsI GCATC 3 cut(s) 67, 111, 442
Bpu10I CCTNAGC 2 cut(s) 159, 339
BpuEI CTTGAG 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 469, 630, 772
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 2 cut(s) 431, 686
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 737
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 4 cut(s) 44, 296, 426, 631
BseDI CCNNGG 3 cut(s) 469, 630, 772
BseGI GGATG 2 cut(s) 82, 154
BseLI CCNNNNNNNGG 2 cut(s) 431, 686
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 173, 330
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 1 cut(s) 654
BseXI GCAGC 3 cut(s) 67, 406, 538
BshFI GGCC 3 cut(s) 42, 47, 435
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 3 cut(s) 48, 98, 599
BslI CCNNNNNNNGG 2 cut(s) 431, 686
BsnI GGCC 3 cut(s) 42, 47, 435
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 345, 497
Bsp143I GATC 2 cut(s) 328, 671
Bsp19I CCATGG 1 cut(s) 772
BspACI CCGC 1 cut(s) 588
BspANI GGCC 3 cut(s) 42, 47, 435
BspCNI CTCAG 2 cut(s) 172, 331
BspLI GGNNCC 2 cut(s) 319, 436
BspMAI CTGCAG 3 cut(s) 396, 544, 556
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 1 cut(s) 679
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 737
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 469, 630, 772
BssMI GATC 2 cut(s) 328, 671
BssT1I CCWWGG 2 cut(s) 469, 772
Bst2UI CCWGG 4 cut(s) 44, 296, 426, 631
Bst6I CTCTTC 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 462, 586
BstDEI CTNAG 3 cut(s) 159, 184, 339
BstDSI CCRYGG 1 cut(s) 772
BstF5I GGATG 2 cut(s) 82, 154
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 3 cut(s) 34, 165, 252
BstKTI GATC 2 cut(s) 331, 674
BstMBI GATC 2 cut(s) 328, 671
BstMWI GCNNNNNNNGC 4 cut(s) 340, 461, 548, 557
BstNI CCWGG 4 cut(s) 44, 296, 426, 631
BstNSI RCATGY 3 cut(s) 192, 588, 730
BstSCI CCNGG 5 cut(s) 42, 97, 294, 424, 629
BstSFI CTRYAG 3 cut(s) 392, 540, 552
BstV1I GCAGC 3 cut(s) 67, 406, 538
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 3 cut(s) 42, 47, 435
BtgI CCRYGG 1 cut(s) 772
BtsCI GGATG 2 cut(s) 82, 154
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 2 cut(s) 462, 586
CfoI GCGC 3 cut(s) 34, 165, 252
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 3 cut(s) 434, 473, 615
Csp6I GTAC 4 cut(s) 95, 209, 730, 769
CviQI GTAC 4 cut(s) 95, 209, 730, 769
DdeI CTNAG 3 cut(s) 159, 184, 339
DpnI GATC 2 cut(s) 330, 673
DpnII GATC 2 cut(s) 328, 671
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 469, 772
Eco24I GRGCYC 2 cut(s) 345, 497
Eco47I GGWCC 2 cut(s) 473, 615
Eco47III AGCGCT 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 473
EcoRII CCWGG 4 cut(s) 42, 294, 424, 629
EcoT14I CCWWGG 2 cut(s) 469, 772
EcoT38I GRGCYC 2 cut(s) 345, 497
ErhI CCWWGG 2 cut(s) 469, 772
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 4 cut(s) 81, 395, 552, 589
FokI GGATG 2 cut(s) 89, 141
FriOI GRGCYC 2 cut(s) 345, 497
Fsp4HI GCNGC 4 cut(s) 81, 395, 552, 589
FspBI CTAG 2 cut(s) 275, 314
FspI TGCGCA 2 cut(s) 33, 251
GlaI GCGC 3 cut(s) 33, 164, 251
GluI GCNGC 4 cut(s) 81, 395, 552, 589
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 3 cut(s) 42, 47, 435
HapII CCGG 3 cut(s) 48, 98, 599
HhaI GCGC 3 cut(s) 34, 165, 252
Hin6I GCGC 3 cut(s) 32, 163, 250
HinP1I GCGC 3 cut(s) 32, 163, 250
HinfI GANTC 1 cut(s) 257
HpaII CCGG 3 cut(s) 48, 98, 599
HphI GGTGA 3 cut(s) 102, 214, 519
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 3 cut(s) 338, 401, 516
Hpy8I GTNNAC 1 cut(s) 110
HpyCH4V TGCA 7 cut(s) 54, 244, 372, 394, 542, 554, 726
HpyF10VI GCNNNNNNNGC 4 cut(s) 340, 461, 548, 557
HpyF3I CTNAG 3 cut(s) 159, 184, 339
HspAI GCGC 3 cut(s) 32, 163, 250
Kzo9I GATC 2 cut(s) 328, 671
LmnI GCTCC 1 cut(s) 317
Lsp1109I GCAGC 3 cut(s) 67, 406, 538
LweI GCATC 3 cut(s) 67, 111, 442
MaeI CTAG 2 cut(s) 275, 314
MaeIII GTNAC 1 cut(s) 815
MalI GATC 2 cut(s) 330, 673
MboI GATC 2 cut(s) 328, 671
MboII GAAGA 2 cut(s) 172, 833
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 345, 497
MluCI AATT 5 cut(s) 57, 303, 520, 824, 828
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 4 cut(s) 117, 147, 606, 827
MslI CAYNNNNRTG 2 cut(s) 14, 505
MspI CCGG 3 cut(s) 48, 98, 599
MspR9I CCNGG 5 cut(s) 44, 99, 296, 426, 631
MvaI CCWGG 4 cut(s) 44, 296, 426, 631
MwoI GCNNNNNNNGC 4 cut(s) 340, 461, 548, 557
NciI CCSGG 1 cut(s) 99
NcoI CCATGG 1 cut(s) 772
NdeI CATATG 1 cut(s) 835
NdeII GATC 2 cut(s) 328, 671
NlaIV GGNNCC 2 cut(s) 319, 436
NsbI TGCGCA 2 cut(s) 33, 251
NspI RCATGY 3 cut(s) 192, 588, 730
PaeI GCATGC 1 cut(s) 588
PaeR7I CTCGAG 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 828
PflMI CCANNNNNTGG 1 cut(s) 431
PkrI GCNGC 4 cut(s) 82, 396, 553, 590
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PpuMI RGGWCCY 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 827
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 473
Psp6I CCWGG 4 cut(s) 42, 294, 424, 629
PspGI CCWGG 4 cut(s) 42, 294, 424, 629
PspN4I GGNNCC 2 cut(s) 319, 436
PspPI GGNCC 3 cut(s) 434, 473, 615
PspPPI RGGWCCY 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 170
PstI CTGCAG 3 cut(s) 396, 544, 556
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 4 cut(s) 96, 210, 731, 770
RsaNI GTAC 4 cut(s) 95, 209, 730, 769
RseI CAYNNNNRTG 2 cut(s) 14, 505
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 4 cut(s) 117, 147, 606, 827
SatI GCNGC 4 cut(s) 81, 395, 552, 589
Sau3AI GATC 2 cut(s) 328, 671
Sau96I GGNCC 3 cut(s) 434, 473, 615
ScaI AGTACT 1 cut(s) 210
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 5 cut(s) 44, 99, 296, 426, 631
SduI GDGCHC 2 cut(s) 345, 497
SfaNI GCATC 3 cut(s) 67, 111, 442
SfcI CTRYAG 3 cut(s) 392, 540, 552
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 2 cut(s) 473, 615
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 2 cut(s) 14, 505
SmlI CTYRAG 2 cut(s) 138, 170
SmoI CTYRAG 2 cut(s) 138, 170
SphI GCATGC 1 cut(s) 588
Sse9I AATT 5 cut(s) 57, 303, 520, 824, 828
SsiI CCGC 1 cut(s) 588
SspI AATATT 1 cut(s) 444
SspMI CTAG 2 cut(s) 275, 314
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 5 cut(s) 42, 97, 294, 424, 629
StyI CCWWGG 2 cut(s) 469, 772
TaqI TCGA 1 cut(s) 171
TasI AATT 5 cut(s) 57, 303, 520, 824, 828
TatI WGTACW 2 cut(s) 208, 729
TauI GCSGC 1 cut(s) 591
Tru1I TTAA 4 cut(s) 117, 147, 606, 827
Tru9I TTAA 4 cut(s) 117, 147, 606, 827
TseI GCWGC 3 cut(s) 80, 394, 551
TspDTI ATGAA 4 cut(s) 513, 561, 801, 852
TspGWI ACGGA 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 431
VpaK11BI GGWCC 2 cut(s) 473, 615
VspI ATTAAT 1 cut(s) 827
XapI RAATTY 1 cut(s) 520
XceI RCATGY 3 cut(s) 192, 588, 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 2 cut(s) 275, 314
ZrmI AGTACT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.