FvH4_1g01491

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007219
Physical Location & Seq
Forward (+)
794064 .. 796344
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01491.t1

Sequence Viewer

Length: 1254 bp
ATGCGGGCGCCTTCACCGCTTGAAGTGAGCTCAGCTTCTTCTTCTTCTCCTTTCCTCTTCTCTCAGCCTCAGATTCCTCCTCGTCTTCGCTACGCCGTCCTCGGCGCTGGCTTCGCCGGCCTCTCGGTCGCTTACAACTTGCTGAAGCAGAGTCCCAAGGACTCGAATCTCCGCATCGACGTTTTCGATGAGGTCGGAATCGGCGGCGGCGCTTCCGGCGTCTCCGGCGGCCTTCTTCATCCTTACTCTCCCAAAGCCAAGCTCTTATGGCGCGCCGCCGAGTGCTGGGACGAGGCTCTGCGGCTCTTGAACGTCGCCGAGGCTGCCGTGCCTGATGCTGCTGCGATTGTTAGAAGAAGGGGAATCTTACGACCAGCTTTGAGCATGAAGAACATGATGGTGCTGGAAGATAATGCCCATAATGGCCTCGCCGGTTGCAGAATTGAAACCATTGACGAGGATGCTGCACAAAATCTTGTACCCGGCATCTGTTCACCTTTTAATACTTCTTTCTATATGCCTCAAGCTGTTAATGTCCATCCTCAGCGCTATCTCGAGGCGCTCTTCTTAGCATGTCAAACTTTGGTAAAAGAACTTTGTGCTTCTGGCTTGGGGGTAAAAGAATTGCATTTGCACAAGGTCTCCATTCAAAAACTGCTTGAATTGGAAGGGGAATACGAGGCAGTAATTGTGTGTCTAGGAGCCAAAGCAGATCTGCTTCCTGAGCTCTCTGGGAGGCTTCCATTGAGGACATGCAGAGGTGTCGTTGCTCATCTGCAGCTTCCTGATAATATGGGAGGGGAGTATCCAGGCTTTGGCCCCTCAATATTGTCAGATGCTTGGCTTGCTGTTCAAGGACCTCGTAGTTTGTATATGGGCTCAACATGGGAATGGAAATCAAGAAATTCATCACCAAATGTATCTGCAGATGAAGCAGCAGAAGCCCTTAAAGAGCTTCTACCAAAGGCATGCCGCATTTATCCAGTTGTAAGAGATTGGACCTTTATCGGAGCCAGAGCTGGCCTTAGAGCAATGCCACCACTCACTCCTCATGGGTCACTTCCTCTGTTGGGTTGTGTCAATAACATTATAGGTCTGAATCAAACTTGCAAGTACTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTTCCATGGTTGGCTAGGGAAGTTGATGGCACAAGCTGTGCTTTCGTGTAACGAAGATTTGATTCCATATGAACTAACCTCATGGAAAAGAACAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

418

Amino Acids

45.25

Weight (kDa)

8.24

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 31 - 395 2.1e-40 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 815
AccII CGCG 1 cut(s) 273
AciI CCGC 9 cut(s) 4, 17, 172, 204, 207, 228, 276, 301, 973
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 2 cut(s) 8, 219
AfaI GTAC 2 cut(s) 480, 1115
AfeI AGCGCT 1 cut(s) 548
AfiI CCNNNNNNNGG 3 cut(s) 285, 815, 1070
AgsI TTSAA 7 cut(s) 23, 310, 446, 650, 662, 854, 1140
AjnI CCWGG 1 cut(s) 808
Alw21I GWGCWC 2 cut(s) 32, 729
Alw26I GTCTC 2 cut(s) 226, 646
Ama87I CYCGRG 1 cut(s) 554
Aor51HI AGCGCT 1 cut(s) 548
AoxI GGCC 5 cut(s) 118, 229, 424, 817, 1021
ApeKI GCWGC 6 cut(s) 323, 338, 341, 464, 778, 935
ApoI RAATTY 1 cut(s) 904
AscI GGCGCGCC 1 cut(s) 271
AspLEI GCGC 7 cut(s) 10, 107, 212, 273, 275, 549, 562
AspS9I GGNCC 3 cut(s) 818, 857, 999
AsuC2I CCSGG 1 cut(s) 483
AsuHPI GGTGA 3 cut(s) 6, 486, 903
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 2 cut(s) 857, 999
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 3 cut(s) 32, 729, 881
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 32, 729
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 6 cut(s) 310, 325, 328, 451, 790, 947
BccI CCATC 3 cut(s) 391, 546, 1171
BceAI ACGGC 2 cut(s) 80, 311
BcgI CGANNNNNNTGC 4 cut(s) 446, 480, 745, 779
BciT130I CCWGG 1 cut(s) 810
BciVI GTATCC 1 cut(s) 816
BcnI CCSGG 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 226, 646
BfaI CTAG 2 cut(s) 698, 1166
BfmI CTRYAG 2 cut(s) 776, 924
BfoI RGCGCY 5 cut(s) 11, 108, 213, 550, 563
BfuI GTATCC 1 cut(s) 816
BglII AGATCT 1 cut(s) 712
BlpI GCTNAGC 1 cut(s) 31
BmcAI AGTACT 1 cut(s) 1115
Bme1390I CCNGG 2 cut(s) 483, 810
Bme18I GGWCC 2 cut(s) 857, 999
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 3 cut(s) 818, 857, 999
BmiI GGNNCC 4 cut(s) 9, 703, 820, 1012
BmrFI CCNGG 2 cut(s) 483, 810
BmsI GCATC 5 cut(s) 183, 325, 451, 495, 826
BpiI GAAGAC 1 cut(s) 77
Bpu10I CCTNAGC 2 cut(s) 543, 723
Bpu1102I GCTNAGC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 507
BpuMI CCSGG 1 cut(s) 483
BsaHI GRCGYC 2 cut(s) 8, 219
BsaI GGTCTC 1 cut(s) 646
BsaJI CCNNGG 4 cut(s) 100, 156, 318, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 815, 1070
Bse118I RCCGGY 2 cut(s) 116, 431
Bse1I ACTGG 2 cut(s) 983, 1121
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 4 cut(s) 100, 156, 318, 1156
BseGI GGATG 3 cut(s) 238, 466, 538
BseLI CCNNNNNNNGG 3 cut(s) 285, 815, 1070
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 5 cut(s) 45, 77, 83, 557, 714
BseNI ACTGG 2 cut(s) 983, 1121
BsePI GCGCGC 1 cut(s) 271
BseRI GAGGAG 2 cut(s) 69, 1038
BseXI GCAGC 6 cut(s) 310, 325, 328, 451, 790, 947
BseYI CCCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 5 cut(s) 120, 231, 426, 819, 1023
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 32, 729
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 5 cut(s) 117, 216, 225, 432, 483
BslFI GGGAC 2 cut(s) 138, 302
BslI CCNNNNNNNGG 3 cut(s) 285, 815, 1070
BsmAI GTCTC 2 cut(s) 226, 646
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 2 cut(s) 138, 302
BsnI GGCC 5 cut(s) 120, 231, 426, 819, 1023
Bso31I GGTCTC 1 cut(s) 646
BsoBI CYCGRG 1 cut(s) 554
Bsp1286I GDGCHC 3 cut(s) 32, 729, 881
Bsp143I GATC 1 cut(s) 712
Bsp1720I GCTNAGC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 1156
BspACI CCGC 9 cut(s) 4, 17, 172, 204, 207, 228, 276, 301, 973
BspANI GGCC 5 cut(s) 120, 231, 426, 819, 1023
BspCNI CTCAG 5 cut(s) 44, 76, 82, 556, 715
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 4 cut(s) 9, 703, 820, 1012
BspMAI CTGCAG 2 cut(s) 780, 928
BspQI GCTCTTC 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 2 cut(s) 116, 431
BsrI ACTGG 2 cut(s) 983, 1121
BssAI RCCGGY 2 cut(s) 116, 431
BssECI CCNNGG 4 cut(s) 100, 156, 318, 1156
BssHII GCGCGC 1 cut(s) 271
BssMI GATC 1 cut(s) 712
BssNI GRCGYC 2 cut(s) 8, 219
BssT1I CCWWGG 2 cut(s) 156, 1156
Bst2UI CCWGG 1 cut(s) 810
Bst6I CTCTTC 2 cut(s) 62, 569
BstACI GRCGYC 2 cut(s) 8, 219
BstC8I GCNNGC 7 cut(s) 6, 109, 118, 273, 846, 970, 1021
BstDEI CTNAG 7 cut(s) 31, 63, 69, 543, 568, 723, 1025
BstDSI CCRYGG 1 cut(s) 1156
BstF5I GGATG 3 cut(s) 238, 466, 538
BstFNI CGCG 1 cut(s) 273
BstH2I RGCGCY 5 cut(s) 11, 108, 213, 550, 563
BstHHI GCGC 7 cut(s) 10, 107, 212, 273, 275, 549, 562
BstKTI GATC 1 cut(s) 715
BstMAI GTCTC 2 cut(s) 226, 646
BstMBI GATC 1 cut(s) 712
BstMCI CGRYCG 1 cut(s) 129
BstNI CCWGG 1 cut(s) 810
BstNSI RCATGY 3 cut(s) 576, 756, 972
BstSCI CCNGG 2 cut(s) 481, 808
BstSFI CTRYAG 2 cut(s) 776, 924
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 6 cut(s) 310, 325, 328, 451, 790, 947
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BsuI GTATCC 1 cut(s) 816
BsuRI GGCC 5 cut(s) 120, 231, 426, 819, 1023
BtgI CCRYGG 1 cut(s) 1156
BtsCI GGATG 3 cut(s) 238, 466, 538
Cac8I GCNNGC 7 cut(s) 6, 109, 118, 273, 846, 970, 1021
CfoI GCGC 7 cut(s) 10, 107, 212, 273, 275, 549, 562
Cfr10I RCCGGY 2 cut(s) 116, 431
Cfr13I GGNCC 3 cut(s) 818, 857, 999
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 479, 1114
CviAII CATG 9 cut(s) 385, 394, 573, 753, 885, 969, 1052, 1157, 1233
CviQI GTAC 2 cut(s) 479, 1114
DdeI CTNAG 7 cut(s) 31, 63, 69, 543, 568, 723, 1025
DinI GGCGCC 1 cut(s) 9
DpnI GATC 1 cut(s) 714
DpnII GATC 1 cut(s) 712
Eam1104I CTCTTC 2 cut(s) 62, 569
EarI CTCTTC 2 cut(s) 62, 569
Ecl136II GAGCTC 2 cut(s) 30, 727
Eco130I CCWWGG 2 cut(s) 156, 1156
Eco24I GRGCYC 3 cut(s) 32, 729, 881
Eco31I GGTCTC 1 cut(s) 646
Eco47I GGWCC 2 cut(s) 857, 999
Eco47III AGCGCT 1 cut(s) 548
Eco53kI GAGCTC 2 cut(s) 30, 727
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 554
EcoICRI GAGCTC 2 cut(s) 30, 727
EcoO109I RGGNCCY 1 cut(s) 857
EcoRII CCWGG 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 156, 1156
EcoT38I GRGCYC 3 cut(s) 32, 729, 881
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 2 cut(s) 156, 1156
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 9 cut(s) 388, 397, 576, 756, 888, 972, 1055, 1160, 1236
FalI AAGNNNNNCTT 2 cut(s) 1176, 1208
FaqI GGGAC 2 cut(s) 138, 302
FatI CATG 9 cut(s) 384, 393, 572, 752, 884, 968, 1051, 1156, 1232
FauNDI CATATG 1 cut(s) 1219
FokI GGATG 3 cut(s) 225, 473, 525
FriOI GRGCYC 3 cut(s) 32, 729, 881
FspBI CTAG 2 cut(s) 698, 1166
GlaI GCGC 7 cut(s) 9, 106, 211, 272, 274, 548, 561
GsaI CCCAGC 1 cut(s) 289
HaeII RGCGCY 5 cut(s) 11, 108, 213, 550, 563
HaeIII GGCC 5 cut(s) 120, 231, 426, 819, 1023
HapII CCGG 5 cut(s) 117, 216, 225, 432, 483
HgaI GACGC 1 cut(s) 208
HhaI GCGC 7 cut(s) 10, 107, 212, 273, 275, 549, 562
Hin1I GRCGYC 2 cut(s) 8, 219
Hin1II CATG 9 cut(s) 388, 397, 576, 756, 888, 972, 1055, 1160, 1236
Hin6I GCGC 7 cut(s) 8, 105, 210, 271, 273, 547, 560
HinP1I GCGC 7 cut(s) 8, 105, 210, 271, 273, 547, 560
HinfI GANTC 8 cut(s) 73, 151, 161, 166, 198, 363, 1099, 1213
HpaII CCGG 5 cut(s) 117, 216, 225, 432, 483
HphI GGTGA 3 cut(s) 6, 486, 903
Hpy166II GTNNAC 1 cut(s) 494
Hpy188I TCNGA 5 cut(s) 72, 197, 835, 1010, 1098
Hpy188III TCNNGA 5 cut(s) 307, 554, 722, 785, 900
Hpy8I GTNNAC 1 cut(s) 494
Hpy99I CGWCG 2 cut(s) 182, 317
HpyAV CCTTC 4 cut(s) 21, 242, 351, 662
HpyCH4IV ACGT 2 cut(s) 180, 312
HpyCH4V TGCA 8 cut(s) 438, 467, 628, 634, 756, 778, 926, 1110
HpyF3I CTNAG 7 cut(s) 31, 63, 69, 543, 568, 723, 1025
HpySE526I ACGT 2 cut(s) 180, 312
Hsp92I GRCGYC 2 cut(s) 8, 219
Hsp92II CATG 9 cut(s) 388, 397, 576, 756, 888, 972, 1055, 1160, 1236
HspAI GCGC 7 cut(s) 8, 105, 210, 271, 273, 547, 560
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 712
LguI GCTCTTC 1 cut(s) 569
LmnI GCTCC 2 cut(s) 701, 1010
Lsp1109I GCAGC 6 cut(s) 310, 325, 328, 451, 790, 947
LweI GCATC 5 cut(s) 183, 325, 451, 495, 826
MaeI CTAG 2 cut(s) 698, 1166
MaeII ACGT 2 cut(s) 180, 312
MaeIII GTNAC 2 cut(s) 1056, 1199
MalI GATC 1 cut(s) 714
MboI GATC 1 cut(s) 712
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 3 cut(s) 32, 729, 881
MluCI AATT 5 cut(s) 441, 623, 662, 687, 904
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 2 cut(s) 155, 160
MmeI TCCRAC 1 cut(s) 175
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 3 cut(s) 501, 531, 948
MslI CAYNNNNRTG 2 cut(s) 398, 889
MspI CCGG 5 cut(s) 117, 216, 225, 432, 483
MspR9I CCNGG 2 cut(s) 483, 810
MvaI CCWGG 1 cut(s) 810
MvnI CGCG 1 cut(s) 273
NaeI GCCGGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 1 cut(s) 483
NcoI CCATGG 1 cut(s) 1156
NdeI CATATG 1 cut(s) 1219
NdeII GATC 1 cut(s) 712
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 9 cut(s) 388, 397, 576, 756, 888, 972, 1055, 1160, 1236
NlaIV GGNNCC 4 cut(s) 9, 703, 820, 1012
NmeAIII GCCGAG 3 cut(s) 81, 304, 343
NmuCI GTSAC 1 cut(s) 1056
NspI RCATGY 3 cut(s) 576, 756, 972
PaeI GCATGC 1 cut(s) 972
PaeR7I CTCGAG 1 cut(s) 554
PalAI GGCGCGCC 1 cut(s) 271
PauI GCGCGC 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 569
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 6 cut(s) 73, 166, 198, 363, 1099, 1213
PflMI CCANNNNNTGG 1 cut(s) 815
PleI GAGTC 2 cut(s) 155, 159
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 2 cut(s) 155, 159
PpuMI RGGWCCY 1 cut(s) 857
Psp124BI GAGCTC 2 cut(s) 32, 729
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 808
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 4 cut(s) 9, 703, 820, 1012
PspPI GGNCC 3 cut(s) 818, 857, 999
PspPPI RGGWCCY 1 cut(s) 857
PstI CTGCAG 2 cut(s) 780, 928
PsuI RGATCY 1 cut(s) 712
PteI GCGCGC 1 cut(s) 271
RsaI GTAC 2 cut(s) 480, 1115
RsaNI GTAC 2 cut(s) 479, 1114
RseI CAYNNNNRTG 2 cut(s) 398, 889
SacI GAGCTC 2 cut(s) 32, 729
SapI GCTCTTC 1 cut(s) 569
SaqAI TTAA 3 cut(s) 501, 531, 948
Sau3AI GATC 1 cut(s) 712
Sau96I GGNCC 3 cut(s) 818, 857, 999
ScaI AGTACT 1 cut(s) 1115
SchI GAGTC 2 cut(s) 155, 160
ScrFI CCNGG 2 cut(s) 483, 810
SduI GDGCHC 3 cut(s) 32, 729, 881
SfaNI GCATC 5 cut(s) 183, 325, 451, 495, 826
SfcI CTRYAG 2 cut(s) 776, 924
SfoI GGCGCC 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 554
SgsI GGCGCGCC 1 cut(s) 271
SinI GGWCC 2 cut(s) 857, 999
SlaI CTCGAG 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 398, 889
SmlI CTYRAG 2 cut(s) 522, 554
SmoI CTYRAG 2 cut(s) 522, 554
SphI GCATGC 1 cut(s) 972
Sse9I AATT 5 cut(s) 441, 623, 662, 687, 904
SsiI CCGC 9 cut(s) 4, 17, 172, 204, 207, 228, 276, 301, 973
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 828
SspMI CTAG 2 cut(s) 698, 1166
SstI GAGCTC 2 cut(s) 32, 729
StyD4I CCNGG 2 cut(s) 481, 808
StyI CCWWGG 2 cut(s) 156, 1156
TaiI ACGT 2 cut(s) 183, 315
TaqI TCGA 4 cut(s) 164, 177, 186, 555
TaqII GACCGA 1 cut(s) 115
TasI AATT 5 cut(s) 441, 623, 662, 687, 904
TatI WGTACW 1 cut(s) 1113
TauI GCSGC 6 cut(s) 207, 210, 231, 278, 304, 975
TfiI GAWTC 6 cut(s) 73, 166, 198, 363, 1099, 1213
Tru1I TTAA 3 cut(s) 501, 531, 948
Tru9I TTAA 3 cut(s) 501, 531, 948
TseFI GTSAC 1 cut(s) 1056
TseI GCWGC 6 cut(s) 323, 338, 341, 464, 778, 935
Tsp45I GTSAC 1 cut(s) 1056
TspDTI ATGAA 5 cut(s) 227, 401, 897, 945, 1236
Van91I CCANNNNNTGG 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 857, 999
XapI RAATTY 1 cut(s) 904
XceI RCATGY 3 cut(s) 576, 756, 972
XhoI CTCGAG 1 cut(s) 554
XspI CTAG 2 cut(s) 698, 1166
ZrmI AGTACT 1 cut(s) 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.