MD02G1011800.v1.1

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007219
Physical Location & Seq
Reverse (-)
753513 .. 755926
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1011800.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGCGTCTTCTTCTTCTTGTTCTGTTGGGTCACCGAGTTCTCTGACTCAGCTCCACCGTCCTCCTCCGAGTCTGAGTCCGAGTTGTGTGAGGTACGCAGTGCTCGGGGCTGGCTTCGCCGGCCTCTCCGTGGCGTGGCACTTGCTAAAGCAGAGTCCGAAAGACTCCAACATCCGAGTCGACATATACGATGAAGCTGGAATCGGCGGCGGCGCGTCCGGGGTTTCGGGTGGGCTTCTTCACCCTTATTCCCCTAAAGCCAAGCTTCTATGGCGTGCTGCCGACTGCTGGAGTGAGTCCTTGAACCTTTTGAGCGCTGCTGAAGCCGCCGTGGCTTCCGCTGCTCAAACTTCTGACGGCCCAACGCCAATTGTTAGAAGAAGGGGCATCTTGAGACCAGCCCTGAGCCTGAAGAACTTGGTGTTGTTGAAAGACAATGCCCAGAGTTGCCTTGCTAGTTGCCGAATAGAAACCCTTGACAACGATGCTGCCCAAAATCTTGTACCTGGCATATCTCCACCTCTTGATGCCTCCTTTTATATGCCTGAAGCTGTTAATATTCATCCTCAACGCTACTTACAGGCACTGTTCTTAGCATGTCAAAATTTGGTGAAGGAACTTCGTGCTTCTGGCACCGGAGTCAAAGAATTGCATTTGCACAAGAGTTCTGTCCACAAACTCGTAGACCTGGAAGGGGAGTACCAGGCAGTAATAGTATGCCTTGGCGCCAAAGCAGACATGCTTCCTGAACTCTGCGGGAGGCTTCCGTTGAGGACGTGCAGAGGTGTTGTTGCACATCTTCAGCTTCCTGATGATATAGGAGAGGAGTATCCGAGCCTTGGCCCCTCGATATTGTCAGATGCTTGGCTTGCTGTCCAAGGTCCTCGGAGTTTATACATGGGCTCAACATGGGAATGGAAATCAAGAAATTCATCACCGGATGTTTCCGCAGAAGAAGCTGGGGAAGCCCTTAAAGAGCTTCTGCCAAAGGCACTTGCCGTTTATCCTGTCCTAAAAGATTGGAAGTTTGCCGGAGCCAGGGCTGGCCTGAGGGCAATGCCGCCGCTCACTCCTCACGGCTCGCTCCCCCTTTTGGGCTGTGTCGATGACATCATAGGCGAAAACCGCAGCTCCAACTACTGGTTATTTGGAGGGCTTGGTTCACGGGGATTGTTGTACCATGGTTGGCTTGGGAAGTTGATGGCACAGGCTGTGCTTTCATGTAATGAAGAATTAATCCCGCATGAATTAACCTCTTGGAAGAAATTAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

426

Amino Acids

45.57

Weight (kDa)

7.57

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 32 - 403 3.9e-43 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 632, 725
AccB7I CCANNNNNTGG 1 cut(s) 1140
AccBSI CCGCTC 1 cut(s) 1066
AccI GTMKAC 2 cut(s) 180, 684
AccII CGCG 1 cut(s) 215
AcsI RAATTY 2 cut(s) 604, 928
AcuI CTGAAG 4 cut(s) 342, 431, 567, 785
AcyI GRCGYC 2 cut(s) 5, 726
AfaI GTAC 4 cut(s) 95, 504, 701, 1178
AfeI AGCGCT 1 cut(s) 316
AfiI CCNNNNNNNGG 9 cut(s) 130, 135, 288, 694, 839, 1038, 1093, 1094, 1140
AgsI TTSAA 2 cut(s) 304, 430
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 4 cut(s) 505, 687, 702, 1037
AluBI AGCT 8 cut(s) 52, 197, 265, 551, 805, 959, 979, 1131
AluI AGCT 8 cut(s) 52, 197, 265, 551, 805, 959, 979, 1131
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 586
Ama87I CYCGRG 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 316
AoxI GGCC 4 cut(s) 121, 358, 841, 1045
ApeKI GCWGC 5 cut(s) 278, 317, 341, 488, 1128
ApoI RAATTY 2 cut(s) 604, 928
AseI ATTAAT 1 cut(s) 1235
AspLEI GCGC 3 cut(s) 215, 317, 728
AspS9I GGNCC 3 cut(s) 359, 842, 881
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 4 cut(s) 24, 233, 622, 927
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 881
AxyI CCTNAGG 1 cut(s) 1049
BanI GGYRCC 2 cut(s) 632, 725
BanII GRGCYC 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 5 cut(s) 265, 304, 328, 475, 1140
BccI CCATC 1 cut(s) 1195
BceAI ACGGC 4 cut(s) 314, 373, 983, 1093
BciT130I CCWGG 4 cut(s) 507, 689, 704, 1039
BciVI GTATCC 1 cut(s) 840
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 1 cut(s) 456
BfoI RGCGCY 2 cut(s) 318, 729
BfuI GTATCC 1 cut(s) 840
BglI GCCNNNNNGGC 1 cut(s) 332
Bme1390I CCNGG 5 cut(s) 220, 507, 689, 704, 1039
Bme18I GGWCC 1 cut(s) 881
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 3 cut(s) 359, 842, 881
BmiI GGNNCC 4 cut(s) 634, 727, 844, 1036
BmrFI CCNGG 5 cut(s) 220, 507, 689, 704, 1039
BmsI GCATC 4 cut(s) 396, 475, 517, 850
BpmI CTGGAG 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 412
BpuMI CCSGG 1 cut(s) 220
BsaHI GRCGYC 2 cut(s) 5, 726
BsaI GGTCTC 1 cut(s) 388
BsaJI CCNNGG 9 cut(s) 129, 219, 330, 721, 838, 877, 884, 1038, 1180
BsaWI WCCGGW 2 cut(s) 635, 937
BsaXI ACNNNNNCTCC 2 cut(s) 1115, 1145
Bsc4I CCNNNNNNNGG 9 cut(s) 130, 135, 288, 694, 839, 1038, 1093, 1094, 1140
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 1145
Bse21I CCTNAGG 1 cut(s) 1049
Bse3DI GCAATG 1 cut(s) 1062
BseBI CCWGG 4 cut(s) 507, 689, 704, 1039
BseDI CCNNGG 9 cut(s) 129, 219, 330, 721, 838, 877, 884, 1038, 1180
BseGI GGATG 3 cut(s) 171, 562, 946
BseLI CCNNNNNNNGG 9 cut(s) 130, 135, 288, 694, 839, 1038, 1093, 1094, 1140
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 4 cut(s) 62, 65, 395, 1040
BseNI ACTGG 1 cut(s) 1145
BseRI GAGGAG 3 cut(s) 54, 839, 1062
BseXI GCAGC 5 cut(s) 265, 304, 328, 475, 1140
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 799
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 4 cut(s) 123, 360, 843, 1047
BshNI GGYRCC 2 cut(s) 632, 725
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 5 cut(s) 120, 219, 636, 938, 1032
BslI CCNNNNNNNGG 9 cut(s) 130, 135, 288, 694, 839, 1038, 1093, 1094, 1140
BsmAI GTCTC 1 cut(s) 388
BsnI GGCC 4 cut(s) 123, 360, 843, 1047
Bso31I GGTCTC 1 cut(s) 388
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 105, 905
Bsp19I CCATGG 1 cut(s) 1180
BspANI GGCC 4 cut(s) 123, 360, 843, 1047
BspCNI CTCAG 4 cut(s) 61, 66, 396, 1041
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 4 cut(s) 634, 727, 844, 1036
BspT107I GGYRCC 2 cut(s) 632, 725
BspTNI GGTCTC 1 cut(s) 388
BsrBI CCGCTC 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 1 cut(s) 1145
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 9 cut(s) 129, 219, 330, 721, 838, 877, 884, 1038, 1180
BssNI GRCGYC 2 cut(s) 5, 726
BssT1I CCWWGG 4 cut(s) 721, 838, 877, 1180
Bst2UI CCWGG 4 cut(s) 507, 689, 704, 1039
Bst4CI ACNGT 2 cut(s) 59, 588
BstACI GRCGYC 2 cut(s) 5, 726
BstC8I GCNNGC 6 cut(s) 112, 121, 276, 870, 1045, 1082
BstDEI CTNAG 5 cut(s) 48, 74, 404, 592, 1049
BstDSI CCRYGG 3 cut(s) 129, 330, 1180
BstEII GGTNACC 1 cut(s) 30
BstF5I GGATG 3 cut(s) 171, 562, 946
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 2 cut(s) 318, 729
BstHHI GCGC 3 cut(s) 215, 317, 728
BstMAI GTCTC 1 cut(s) 388
BstNI CCWGG 4 cut(s) 507, 689, 704, 1039
BstNSI RCATGY 2 cut(s) 600, 742
BstPI GGTNACC 1 cut(s) 30
BstSCI CCNGG 5 cut(s) 218, 505, 687, 702, 1037
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 5 cut(s) 265, 304, 328, 475, 1140
Bsu36I CCTNAGG 1 cut(s) 1049
BsuI GTATCC 1 cut(s) 840
BsuRI GGCC 4 cut(s) 123, 360, 843, 1047
BtgI CCRYGG 3 cut(s) 129, 330, 1180
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 3 cut(s) 171, 562, 946
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 584
Cac8I GCNNGC 6 cut(s) 112, 121, 276, 870, 1045, 1082
CaiI CAGNNNCTG 1 cut(s) 586
CfoI GCGC 3 cut(s) 215, 317, 728
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 359, 842, 881
CseI GACGC 1 cut(s) 204
Csp6I GTAC 4 cut(s) 94, 503, 700, 1177
CviAII CATG 7 cut(s) 597, 739, 898, 909, 1181, 1221, 1244
CviQI GTAC 4 cut(s) 94, 503, 700, 1177
DdeI CTNAG 5 cut(s) 48, 74, 404, 592, 1049
DinI GGCGCC 1 cut(s) 727
Eco130I CCWWGG 4 cut(s) 721, 838, 877, 1180
Eco24I GRGCYC 1 cut(s) 905
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 881
Eco47III AGCGCT 1 cut(s) 316
Eco57I CTGAAG 4 cut(s) 342, 431, 567, 785
Eco81I CCTNAGG 1 cut(s) 1049
Eco88I CYCGRG 1 cut(s) 104
Eco91I GGTNACC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 881
EcoO65I GGTNACC 1 cut(s) 30
EcoRII CCWGG 4 cut(s) 505, 687, 702, 1037
EcoT14I CCWWGG 4 cut(s) 721, 838, 877, 1180
EcoT38I GRGCYC 1 cut(s) 905
EgeI GGCGCC 1 cut(s) 727
EheI GGCGCC 1 cut(s) 727
ErhI CCWWGG 4 cut(s) 721, 838, 877, 1180
FaeI CATG 7 cut(s) 600, 742, 901, 912, 1184, 1224, 1247
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 7 cut(s) 596, 738, 897, 908, 1180, 1220, 1243
FauI CCCGC 2 cut(s) 749, 1248
FblI GTMKAC 2 cut(s) 180, 684
FokI GGATG 3 cut(s) 158, 549, 953
FriOI GRGCYC 1 cut(s) 905
FspBI CTAG 1 cut(s) 456
GlaI GCGC 3 cut(s) 214, 316, 727
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 310
HaeII RGCGCY 2 cut(s) 318, 729
HaeIII GGCC 4 cut(s) 123, 360, 843, 1047
HapII CCGG 5 cut(s) 120, 219, 636, 938, 1032
HgaI GACGC 1 cut(s) 204
HhaI GCGC 3 cut(s) 215, 317, 728
Hin1I GRCGYC 2 cut(s) 5, 726
Hin1II CATG 7 cut(s) 600, 742, 901, 912, 1184, 1224, 1247
Hin6I GCGC 3 cut(s) 213, 315, 726
HinP1I GCGC 3 cut(s) 213, 315, 726
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 9 cut(s) 46, 70, 76, 154, 164, 177, 201, 296, 639
HpaII CCGG 5 cut(s) 120, 219, 636, 938, 1032
HphI GGTGA 4 cut(s) 24, 233, 622, 927
Hpy166II GTNNAC 4 cut(s) 181, 673, 685, 1163
Hpy188III TCNNGA 5 cut(s) 391, 524, 746, 809, 924
Hpy8I GTNNAC 4 cut(s) 181, 673, 685, 1163
HpyAV CCTTC 3 cut(s) 375, 607, 686
HpyCH4III ACNGT 2 cut(s) 59, 588
HpyCH4IV ACGT 1 cut(s) 776
HpyCH4V TGCA 4 cut(s) 652, 658, 780, 794
HpyF3I CTNAG 5 cut(s) 48, 74, 404, 592, 1049
HpySE526I ACGT 1 cut(s) 776
Hsp92I GRCGYC 2 cut(s) 5, 726
Hsp92II CATG 7 cut(s) 600, 742, 901, 912, 1184, 1224, 1247
HspAI GCGC 3 cut(s) 213, 315, 726
KasI GGCGCC 1 cut(s) 725
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
LmnI GCTCC 4 cut(s) 57, 1034, 1089, 1136
Lsp1109I GCAGC 5 cut(s) 265, 304, 328, 475, 1140
LweI GCATC 4 cut(s) 396, 475, 517, 850
MaeI CTAG 1 cut(s) 456
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 1 cut(s) 30
MbiI CCGCTC 1 cut(s) 1066
MboII GAAGA 9 cut(s) 3, 6, 230, 390, 424, 791, 965, 1241, 1273
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 105, 905
MluCI AATT 7 cut(s) 369, 604, 647, 928, 1232, 1247, 1265
Mly113I GGCGCC 1 cut(s) 726
MlyI GAGTC 8 cut(s) 40, 79, 85, 158, 163, 186, 305, 648
MmeI TCCRAC 2 cut(s) 192, 1158
MroNI GCCGGC 1 cut(s) 119
MseI TTAA 5 cut(s) 555, 972, 1235, 1250, 1268
MslI CAYNNNNRTG 1 cut(s) 913
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 5 cut(s) 120, 219, 636, 938, 1032
MspR9I CCNGG 5 cut(s) 220, 507, 689, 704, 1039
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 4 cut(s) 507, 689, 704, 1039
MvnI CGCG 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 726
NciI CCSGG 1 cut(s) 220
NcoI CCATGG 1 cut(s) 1180
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 7 cut(s) 600, 742, 901, 912, 1184, 1224, 1247
NlaIV GGNNCC 4 cut(s) 634, 727, 844, 1036
NmuCI GTSAC 1 cut(s) 30
NspI RCATGY 2 cut(s) 600, 742
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 121
PfeI GAWTC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 1140
PleI GAGTC 8 cut(s) 40, 78, 84, 158, 162, 185, 304, 647
PluTI GGCGCC 1 cut(s) 729
PpsI GAGTC 8 cut(s) 40, 78, 84, 158, 162, 185, 304, 647
PpuMI RGGWCCY 1 cut(s) 881
PshBI ATTAAT 1 cut(s) 1235
Psp5II RGGWCCY 1 cut(s) 881
Psp6I CCWGG 4 cut(s) 505, 687, 702, 1037
PspEI GGTNACC 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 4 cut(s) 505, 687, 702, 1037
PspN4I GGNNCC 4 cut(s) 634, 727, 844, 1036
PspPI GGNCC 3 cut(s) 359, 842, 881
PspPPI RGGWCCY 1 cut(s) 881
PstNI CAGNNNCTG 1 cut(s) 586
RsaI GTAC 4 cut(s) 95, 504, 701, 1178
RsaNI GTAC 4 cut(s) 94, 503, 700, 1177
RseI CAYNNNNRTG 1 cut(s) 913
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 5 cut(s) 555, 972, 1235, 1250, 1268
Sau96I GGNCC 3 cut(s) 359, 842, 881
SchI GAGTC 8 cut(s) 40, 79, 85, 158, 163, 186, 305, 648
ScrFI CCNGG 5 cut(s) 220, 507, 689, 704, 1039
SduI GDGCHC 2 cut(s) 105, 905
SfaNI GCATC 4 cut(s) 396, 475, 517, 850
SfoI GGCGCC 1 cut(s) 727
SinI GGWCC 1 cut(s) 881
SmiMI CAYNNNNRTG 1 cut(s) 913
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 7 cut(s) 369, 604, 647, 928, 1232, 1247, 1265
SspDI GGCGCC 1 cut(s) 725
SspI AATATT 1 cut(s) 559
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 5 cut(s) 218, 505, 687, 702, 1037
StyI CCWWGG 4 cut(s) 721, 838, 877, 1180
TaaI ACNGT 2 cut(s) 59, 588
TaiI ACGT 1 cut(s) 779
TaqI TCGA 3 cut(s) 180, 848, 1104
TasI AATT 7 cut(s) 369, 604, 647, 928, 1232, 1247, 1265
TauI GCSGC 5 cut(s) 210, 213, 329, 1063, 1066
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 5 cut(s) 555, 972, 1235, 1250, 1268
Tru9I TTAA 5 cut(s) 555, 972, 1235, 1250, 1268
TscAI CASTG 2 cut(s) 105, 591
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 5 cut(s) 278, 317, 341, 488, 1128
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 6 cut(s) 207, 551, 921, 1209, 1242, 1260
TspGWI ACGGA 2 cut(s) 118, 756
TspRI CASTG 2 cut(s) 105, 591
Van91I CCANNNNNTGG 1 cut(s) 1140
VpaK11BI GGWCC 1 cut(s) 881
VspI ATTAAT 1 cut(s) 1235
XapI RAATTY 2 cut(s) 604, 928
XceI RCATGY 2 cut(s) 600, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 1187
XmiI GTMKAC 2 cut(s) 180, 684
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.